Ogataea parapolymorpha (strain ATCC 26012 / BCRC 20466 / JCM 22074 / NRRL Y-7560 / DL-1) (Yeast) (Hansenula polymorpha)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Pichiaceae; Ogataea; Ogataea parapolymorpha

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5338 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|W1QK75|W1QK75_OGAPD Clathrin coat assembly protein OS=Ogataea parapolymorpha (strain ATCC 26012 / BCRC 20466 / JCM 22074 / NRRL Y-7560 / DL-1) OX=871575 GN=HPODL_05008 PE=4 SV=1
MM1 pKa = 7.39SLLRR5 pKa = 11.84NFIDD9 pKa = 3.61ALSPITAYY17 pKa = 10.48AEE19 pKa = 4.16EE20 pKa = 4.39PAEE23 pKa = 4.13EE24 pKa = 4.04PVEE27 pKa = 4.12EE28 pKa = 4.39VEE30 pKa = 4.51EE31 pKa = 4.22EE32 pKa = 4.06ASEE35 pKa = 4.27EE36 pKa = 4.06PQEE39 pKa = 4.16EE40 pKa = 4.38EE41 pKa = 4.88AGGDD45 pKa = 3.48DD46 pKa = 4.56AEE48 pKa = 4.56EE49 pKa = 4.32EE50 pKa = 4.39EE51 pKa = 5.49EE52 pKa = 5.36DD53 pKa = 5.66DD54 pKa = 5.82EE55 pKa = 7.17DD56 pKa = 6.64DD57 pKa = 4.69EE58 pKa = 7.21DD59 pKa = 5.7EE60 pKa = 5.25DD61 pKa = 6.4DD62 pKa = 4.16EE63 pKa = 6.26DD64 pKa = 4.84EE65 pKa = 4.41EE66 pKa = 6.43AGDD69 pKa = 4.48PMDD72 pKa = 5.44ALRR75 pKa = 11.84KK76 pKa = 8.97QCSEE80 pKa = 4.19TKK82 pKa = 9.12EE83 pKa = 4.07CAHH86 pKa = 6.11YY87 pKa = 8.95VHH89 pKa = 7.39HH90 pKa = 5.97YY91 pKa = 7.83QEE93 pKa = 4.48CVEE96 pKa = 4.21RR97 pKa = 11.84VTKK100 pKa = 10.43EE101 pKa = 3.85MEE103 pKa = 3.87EE104 pKa = 3.85PGYY107 pKa = 10.3DD108 pKa = 3.08EE109 pKa = 5.86KK110 pKa = 11.14EE111 pKa = 4.15YY112 pKa = 11.23KK113 pKa = 9.72EE114 pKa = 4.32DD115 pKa = 3.51CVEE118 pKa = 4.17EE119 pKa = 4.55FFHH122 pKa = 6.74LQHH125 pKa = 7.19CINDD129 pKa = 3.77CVAPKK134 pKa = 10.61LFYY137 pKa = 10.45KK138 pKa = 10.84LKK140 pKa = 10.57

Molecular weight:
16.3 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|W1Q7R0|W1Q7R0_OGAPD Protein JSN1 OS=Ogataea parapolymorpha (strain ATCC 26012 / BCRC 20466 / JCM 22074 / NRRL Y-7560 / DL-1) OX=871575 GN=HPODL_02653 PE=4 SV=1
MM1 pKa = 7.49LRR3 pKa = 11.84TIKK6 pKa = 10.35LARR9 pKa = 11.84PISTQRR15 pKa = 11.84EE16 pKa = 3.62RR17 pKa = 11.84RR18 pKa = 11.84FFSTDD23 pKa = 2.86VVSVFEE29 pKa = 4.43SGAEE33 pKa = 4.0LVSAAHH39 pKa = 6.62AASGLPWWAFIPLITVSVRR58 pKa = 11.84TCITLPLAIYY68 pKa = 8.65QRR70 pKa = 11.84RR71 pKa = 11.84GLQKK75 pKa = 10.37QNEE78 pKa = 4.22LRR80 pKa = 11.84PIISAMFPIFKK91 pKa = 10.31LRR93 pKa = 11.84LAARR97 pKa = 11.84AQAAQQSARR106 pKa = 11.84LSKK109 pKa = 10.79SDD111 pKa = 3.64VPIKK115 pKa = 10.24TEE117 pKa = 4.03TEE119 pKa = 3.67QLSANKK125 pKa = 10.0IIVLASKK132 pKa = 10.61EE133 pKa = 3.83RR134 pKa = 11.84MKK136 pKa = 10.57RR137 pKa = 11.84QRR139 pKa = 11.84QIFRR143 pKa = 11.84DD144 pKa = 3.76NGCQSWKK151 pKa = 10.45FLALPALQIPLWITLSQTFRR171 pKa = 11.84VLTGWTNVRR180 pKa = 11.84ATVLDD185 pKa = 3.78PTLSTEE191 pKa = 4.06GLGYY195 pKa = 8.86LTNLTLSDD203 pKa = 4.38PYY205 pKa = 10.85FILPVVLGVTALTNAEE221 pKa = 4.15WNFRR225 pKa = 11.84TADD228 pKa = 3.65LMKK231 pKa = 9.79LTTRR235 pKa = 11.84GVRR238 pKa = 11.84NSLRR242 pKa = 11.84PTAFDD247 pKa = 3.4SVINLTRR254 pKa = 11.84MSICFLVVLSAQAPAALSIYY274 pKa = 9.04WISSNIYY281 pKa = 10.41SLAQNILLNKK291 pKa = 9.34FMPLRR296 pKa = 11.84YY297 pKa = 7.83TPYY300 pKa = 10.67ARR302 pKa = 11.84SAPSTKK308 pKa = 10.23KK309 pKa = 10.45LAGEE313 pKa = 4.19SLVEE317 pKa = 4.26FF318 pKa = 4.95

Molecular weight:
35.68 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5338

0

5338

2502102

48

4775

468.7

52.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.533 ± 0.033

1.257 ± 0.01

5.83 ± 0.023

6.872 ± 0.035

4.368 ± 0.018

5.347 ± 0.031

2.122 ± 0.012

5.726 ± 0.023

6.694 ± 0.03

10.158 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.098 ± 0.012

4.644 ± 0.019

4.616 ± 0.025

4.092 ± 0.022

4.932 ± 0.026

8.328 ± 0.051

5.462 ± 0.022

6.366 ± 0.024

1.073 ± 0.009

3.482 ± 0.019

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski