Ogataea parapolymorpha (strain ATCC 26012 / BCRC 20466 / JCM 22074 / NRRL Y-7560 / DL-1) (Yeast) (Hansenula polymorpha)
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5338 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W1QK75|W1QK75_OGAPD Clathrin coat assembly protein OS=Ogataea parapolymorpha (strain ATCC 26012 / BCRC 20466 / JCM 22074 / NRRL Y-7560 / DL-1) OX=871575 GN=HPODL_05008 PE=4 SV=1
MM1 pKa = 7.39 SLLRR5 pKa = 11.84 NFIDD9 pKa = 3.61 ALSPITAYY17 pKa = 10.48 AEE19 pKa = 4.16 EE20 pKa = 4.39 PAEE23 pKa = 4.13 EE24 pKa = 4.04 PVEE27 pKa = 4.12 EE28 pKa = 4.39 VEE30 pKa = 4.51 EE31 pKa = 4.22 EE32 pKa = 4.06 ASEE35 pKa = 4.27 EE36 pKa = 4.06 PQEE39 pKa = 4.16 EE40 pKa = 4.38 EE41 pKa = 4.88 AGGDD45 pKa = 3.48 DD46 pKa = 4.56 AEE48 pKa = 4.56 EE49 pKa = 4.32 EE50 pKa = 4.39 EE51 pKa = 5.49 EE52 pKa = 5.36 DD53 pKa = 5.66 DD54 pKa = 5.82 EE55 pKa = 7.17 DD56 pKa = 6.64 DD57 pKa = 4.69 EE58 pKa = 7.21 DD59 pKa = 5.7 EE60 pKa = 5.25 DD61 pKa = 6.4 DD62 pKa = 4.16 EE63 pKa = 6.26 DD64 pKa = 4.84 EE65 pKa = 4.41 EE66 pKa = 6.43 AGDD69 pKa = 4.48 PMDD72 pKa = 5.44 ALRR75 pKa = 11.84 KK76 pKa = 8.97 QCSEE80 pKa = 4.19 TKK82 pKa = 9.12 EE83 pKa = 4.07 CAHH86 pKa = 6.11 YY87 pKa = 8.95 VHH89 pKa = 7.39 HH90 pKa = 5.97 YY91 pKa = 7.83 QEE93 pKa = 4.48 CVEE96 pKa = 4.21 RR97 pKa = 11.84 VTKK100 pKa = 10.43 EE101 pKa = 3.85 MEE103 pKa = 3.87 EE104 pKa = 3.85 PGYY107 pKa = 10.3 DD108 pKa = 3.08 EE109 pKa = 5.86 KK110 pKa = 11.14 EE111 pKa = 4.15 YY112 pKa = 11.23 KK113 pKa = 9.72 EE114 pKa = 4.32 DD115 pKa = 3.51 CVEE118 pKa = 4.17 EE119 pKa = 4.55 FFHH122 pKa = 6.74 LQHH125 pKa = 7.19 CINDD129 pKa = 3.77 CVAPKK134 pKa = 10.61 LFYY137 pKa = 10.45 KK138 pKa = 10.84 LKK140 pKa = 10.57
Molecular weight: 16.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.783
IPC2_protein 3.91
IPC_protein 3.859
Toseland 3.694
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.668
Rodwell 3.694
Grimsley 3.605
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.037
Thurlkill 3.706
EMBOSS 3.694
Sillero 3.973
Patrickios 1.85
IPC_peptide 3.808
IPC2_peptide 3.961
IPC2.peptide.svr19 3.859
Protein with the highest isoelectric point:
>tr|W1Q7R0|W1Q7R0_OGAPD Protein JSN1 OS=Ogataea parapolymorpha (strain ATCC 26012 / BCRC 20466 / JCM 22074 / NRRL Y-7560 / DL-1) OX=871575 GN=HPODL_02653 PE=4 SV=1
MM1 pKa = 7.49 LRR3 pKa = 11.84 TIKK6 pKa = 10.35 LARR9 pKa = 11.84 PISTQRR15 pKa = 11.84 EE16 pKa = 3.62 RR17 pKa = 11.84 RR18 pKa = 11.84 FFSTDD23 pKa = 2.86 VVSVFEE29 pKa = 4.43 SGAEE33 pKa = 4.0 LVSAAHH39 pKa = 6.62 AASGLPWWAFIPLITVSVRR58 pKa = 11.84 TCITLPLAIYY68 pKa = 8.65 QRR70 pKa = 11.84 RR71 pKa = 11.84 GLQKK75 pKa = 10.37 QNEE78 pKa = 4.22 LRR80 pKa = 11.84 PIISAMFPIFKK91 pKa = 10.31 LRR93 pKa = 11.84 LAARR97 pKa = 11.84 AQAAQQSARR106 pKa = 11.84 LSKK109 pKa = 10.79 SDD111 pKa = 3.64 VPIKK115 pKa = 10.24 TEE117 pKa = 4.03 TEE119 pKa = 3.67 QLSANKK125 pKa = 10.0 IIVLASKK132 pKa = 10.61 EE133 pKa = 3.83 RR134 pKa = 11.84 MKK136 pKa = 10.57 RR137 pKa = 11.84 QRR139 pKa = 11.84 QIFRR143 pKa = 11.84 DD144 pKa = 3.76 NGCQSWKK151 pKa = 10.45 FLALPALQIPLWITLSQTFRR171 pKa = 11.84 VLTGWTNVRR180 pKa = 11.84 ATVLDD185 pKa = 3.78 PTLSTEE191 pKa = 4.06 GLGYY195 pKa = 8.86 LTNLTLSDD203 pKa = 4.38 PYY205 pKa = 10.85 FILPVVLGVTALTNAEE221 pKa = 4.15 WNFRR225 pKa = 11.84 TADD228 pKa = 3.65 LMKK231 pKa = 9.79 LTTRR235 pKa = 11.84 GVRR238 pKa = 11.84 NSLRR242 pKa = 11.84 PTAFDD247 pKa = 3.4 SVINLTRR254 pKa = 11.84 MSICFLVVLSAQAPAALSIYY274 pKa = 9.04 WISSNIYY281 pKa = 10.41 SLAQNILLNKK291 pKa = 9.34 FMPLRR296 pKa = 11.84 YY297 pKa = 7.83 TPYY300 pKa = 10.67 ARR302 pKa = 11.84 SAPSTKK308 pKa = 10.23 KK309 pKa = 10.45 LAGEE313 pKa = 4.19 SLVEE317 pKa = 4.26 FF318 pKa = 4.95
Molecular weight: 35.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.338
IPC2_protein 9.736
IPC_protein 10.54
Toseland 10.701
ProMoST 10.394
Dawson 10.804
Bjellqvist 10.511
Wikipedia 11.008
Rodwell 11.038
Grimsley 10.847
Solomon 10.906
Lehninger 10.877
Nozaki 10.687
DTASelect 10.511
Thurlkill 10.701
EMBOSS 11.096
Sillero 10.73
Patrickios 10.73
IPC_peptide 10.921
IPC2_peptide 9.487
IPC2.peptide.svr19 8.588
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5338
0
5338
2502102
48
4775
468.7
52.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.533 ± 0.033
1.257 ± 0.01
5.83 ± 0.023
6.872 ± 0.035
4.368 ± 0.018
5.347 ± 0.031
2.122 ± 0.012
5.726 ± 0.023
6.694 ± 0.03
10.158 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.098 ± 0.012
4.644 ± 0.019
4.616 ± 0.025
4.092 ± 0.022
4.932 ± 0.026
8.328 ± 0.051
5.462 ± 0.022
6.366 ± 0.024
1.073 ± 0.009
3.482 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here