Vibrio galatheae
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3604 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F4NJC6|A0A0F4NJC6_9VIBR Terminase_6C domain-containing protein OS=Vibrio galatheae OX=579748 GN=TW81_09850 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 8.8 KK3 pKa = 8.26 TLVALSVLMAATSAQAIEE21 pKa = 5.48 LYY23 pKa = 10.36 NQDD26 pKa = 3.15 GVTVNLSGDD35 pKa = 3.42 VEE37 pKa = 3.96 IRR39 pKa = 11.84 YY40 pKa = 9.83 LKK42 pKa = 10.62 DD43 pKa = 2.85 IGVDD47 pKa = 3.3 QITKK51 pKa = 10.1 QEE53 pKa = 3.85 IDD55 pKa = 3.58 DD56 pKa = 4.31 ADD58 pKa = 3.86 FGFDD62 pKa = 2.66 TRR64 pKa = 11.84 YY65 pKa = 10.5 AVNDD69 pKa = 3.81 DD70 pKa = 4.22 FQVGAYY76 pKa = 10.1 FEE78 pKa = 4.78 FSGDD82 pKa = 3.56 DD83 pKa = 3.31 GDD85 pKa = 3.86 RR86 pKa = 11.84 AVGNTSVGNVYY97 pKa = 10.55 FGFYY101 pKa = 10.05 HH102 pKa = 6.36 NAYY105 pKa = 7.61 GTLKK109 pKa = 10.52 VGKK112 pKa = 9.77 LDD114 pKa = 4.86 TILDD118 pKa = 3.95 DD119 pKa = 4.58 AGIGADD125 pKa = 3.76 YY126 pKa = 10.88 IFGVNSFFTDD136 pKa = 2.83 AGFGGDD142 pKa = 3.03 EE143 pKa = 4.16 AVRR146 pKa = 11.84 YY147 pKa = 10.01 DD148 pKa = 3.76 YY149 pKa = 11.74 DD150 pKa = 3.46 NGSFYY155 pKa = 11.28 AGLGLIQDD163 pKa = 3.78 KK164 pKa = 10.94 HH165 pKa = 7.81 DD166 pKa = 4.74 DD167 pKa = 3.64 GAIGVDD173 pKa = 3.21 GHH175 pKa = 6.27 YY176 pKa = 10.97 VDD178 pKa = 5.49 AKK180 pKa = 9.66 VGYY183 pKa = 9.24 RR184 pKa = 11.84 VAGFDD189 pKa = 3.46 FTVLYY194 pKa = 10.84 GDD196 pKa = 4.21 TEE198 pKa = 4.5 LKK200 pKa = 10.97 GVDD203 pKa = 3.59 SDD205 pKa = 4.92 SNALNKK211 pKa = 10.22 EE212 pKa = 3.99 DD213 pKa = 3.77 TLIAGEE219 pKa = 3.97 VRR221 pKa = 11.84 YY222 pKa = 10.22 AGIEE226 pKa = 3.98 NLNLEE231 pKa = 4.42 LGYY234 pKa = 11.23 YY235 pKa = 10.13 NVDD238 pKa = 3.35 SKK240 pKa = 11.52 PFGGSSTDD248 pKa = 3.36 VNTLAVAADD257 pKa = 3.83 YY258 pKa = 8.47 TWNKK262 pKa = 6.81 VTFAGGFSTTDD273 pKa = 3.2 YY274 pKa = 9.92 STAAAGTDD282 pKa = 3.73 FDD284 pKa = 4.27 QWFLNAGYY292 pKa = 10.42 GIAPNTTVYY301 pKa = 10.99 VEE303 pKa = 4.47 IGDD306 pKa = 3.81 NDD308 pKa = 3.82 KK309 pKa = 11.74 ANTDD313 pKa = 3.17 LGYY316 pKa = 11.05 GVGIKK321 pKa = 10.55 ASFF324 pKa = 3.21
Molecular weight: 34.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.846
IPC_protein 3.897
Toseland 3.656
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.859
Rodwell 3.706
Grimsley 3.554
Solomon 3.897
Lehninger 3.859
Nozaki 4.012
DTASelect 4.317
Thurlkill 3.719
EMBOSS 3.872
Sillero 4.012
Patrickios 1.354
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.89
Protein with the highest isoelectric point:
>tr|A0A0F4NHY2|A0A0F4NHY2_9VIBR Uncharacterized protein OS=Vibrio galatheae OX=579748 GN=TW81_17355 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.5 RR3 pKa = 11.84 TFQPTVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 7.65 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.44 NGRR28 pKa = 11.84 ATINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.74 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSKK44 pKa = 10.84
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3604
0
3604
1164118
31
3040
323.0
35.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.733 ± 0.046
1.017 ± 0.013
5.495 ± 0.036
6.463 ± 0.038
4.061 ± 0.027
6.773 ± 0.034
2.242 ± 0.022
6.322 ± 0.033
5.202 ± 0.035
10.436 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.675 ± 0.022
4.102 ± 0.025
3.842 ± 0.021
4.825 ± 0.036
4.546 ± 0.033
6.621 ± 0.04
5.25 ± 0.024
7.195 ± 0.028
1.226 ± 0.017
2.976 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here