Trichinella sp. T6
Average proteome isoelectric point is 7.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 18636 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0V0XA74|A0A0V0XA74_9BILA Uncharacterized protein OS=Trichinella sp. T6 OX=92179 GN=T06_11651 PE=4 SV=1
MM1 pKa = 7.78 EE2 pKa = 5.25 DD3 pKa = 2.75 WKK5 pKa = 11.1 IFDD8 pKa = 4.19 FDD10 pKa = 3.79 VDD12 pKa = 3.85 LMLWLVMLAMLLHH25 pKa = 5.43 MAKK28 pKa = 10.42 YY29 pKa = 10.45 LLDD32 pKa = 3.73 YY33 pKa = 11.44 LEE35 pKa = 4.54 TFQEE39 pKa = 4.26 KK40 pKa = 10.83 EE41 pKa = 3.96 EE42 pKa = 4.85 LIQLLNEE49 pKa = 3.77 THH51 pKa = 6.37 YY52 pKa = 11.81 SMEE55 pKa = 3.9 QVEE58 pKa = 4.68 QIVEE62 pKa = 4.22 HH63 pKa = 5.41 QQSVDD68 pKa = 3.12 NKK70 pKa = 10.54 DD71 pKa = 4.01 YY72 pKa = 11.01 IGEE75 pKa = 4.15 PYY77 pKa = 10.21 IFYY80 pKa = 10.94 DD81 pKa = 3.27 PDD83 pKa = 4.24 KK84 pKa = 11.38 DD85 pKa = 4.21 VFTLQIDD92 pKa = 4.34 FNNLSEE98 pKa = 4.58 DD99 pKa = 3.47 NCIKK103 pKa = 10.69 VCLAVWNALVNINSDD118 pKa = 3.45 SDD120 pKa = 4.14 SDD122 pKa = 4.73 DD123 pKa = 4.41 DD124 pKa = 5.71 DD125 pKa = 5.98 SEE127 pKa = 4.73 EE128 pKa = 5.05 SDD130 pKa = 3.58 EE131 pKa = 4.16 QQ132 pKa = 3.8
Molecular weight: 15.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.643
IPC_protein 3.643
Toseland 3.427
ProMoST 3.808
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.579
Rodwell 3.478
Grimsley 3.338
Solomon 3.63
Lehninger 3.579
Nozaki 3.757
DTASelect 3.986
Thurlkill 3.49
EMBOSS 3.592
Sillero 3.77
Patrickios 0.985
IPC_peptide 3.617
IPC2_peptide 3.745
IPC2.peptide.svr19 3.703
Protein with the highest isoelectric point:
>tr|A0A0V0WVB8|A0A0V0WVB8_9BILA Uncharacterized protein OS=Trichinella sp. T6 OX=92179 GN=T06_15559 PE=4 SV=1
MM1 pKa = 7.32 ARR3 pKa = 11.84 SARR6 pKa = 11.84 SSSRR10 pKa = 11.84 SRR12 pKa = 11.84 SRR14 pKa = 11.84 SSSRR18 pKa = 11.84 RR19 pKa = 11.84 SMSSRR24 pKa = 11.84 RR25 pKa = 11.84 GSLTRR30 pKa = 11.84 PRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 PISRR38 pKa = 11.84 IQTPKK43 pKa = 10.33 RR44 pKa = 11.84 STRR47 pKa = 11.84 QILVPSSRR55 pKa = 11.84 LKK57 pKa = 9.77 LQKK60 pKa = 10.27 RR61 pKa = 11.84 PSAVQLDD68 pKa = 4.04 GGEE71 pKa = 4.16 APSEE75 pKa = 4.29 IICPTCLQSLRR86 pKa = 11.84 SRR88 pKa = 11.84 SRR90 pKa = 11.84 SKK92 pKa = 10.85 SITRR96 pKa = 11.84 KK97 pKa = 9.61 KK98 pKa = 10.85 SPGKK102 pKa = 9.58 KK103 pKa = 8.93 VSRR106 pKa = 11.84 KK107 pKa = 7.81 VASRR111 pKa = 11.84 PRR113 pKa = 11.84 SKK115 pKa = 10.92 SRR117 pKa = 11.84 GKK119 pKa = 9.94 SRR121 pKa = 11.84 RR122 pKa = 11.84 NKK124 pKa = 9.1 KK125 pKa = 10.28 RR126 pKa = 11.84 SVKK129 pKa = 10.23 KK130 pKa = 10.29 SNQKK134 pKa = 10.42 AVLIKK139 pKa = 9.59 ATKK142 pKa = 9.47 SRR144 pKa = 11.84 RR145 pKa = 11.84 KK146 pKa = 9.6 RR147 pKa = 11.84 SSLKK151 pKa = 10.25 SIQNRR156 pKa = 11.84 SVVV159 pKa = 3.14
Molecular weight: 18.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.426
IPC2_protein 10.921
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14632
4004
18636
8581469
30
14872
460.5
52.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.408 ± 0.015
2.648 ± 0.017
5.223 ± 0.014
6.294 ± 0.02
4.588 ± 0.013
4.975 ± 0.018
2.463 ± 0.007
5.612 ± 0.014
6.081 ± 0.02
9.634 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.411 ± 0.009
5.017 ± 0.013
4.367 ± 0.015
4.264 ± 0.015
5.626 ± 0.018
8.356 ± 0.021
5.305 ± 0.013
6.419 ± 0.014
1.212 ± 0.007
3.095 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here