Paenibacillus sp. 32O-W

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4738 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U2WLG5|A0A0U2WLG5_9BACL Biotin carboxyl carrier protein of acetyl-CoA carboxylase OS=Paenibacillus sp. 32O-W OX=1695218 GN=IJ21_30090 PE=4 SV=1
MM1 pKa = 7.49AFVITEE7 pKa = 3.76ACIDD11 pKa = 3.94EE12 pKa = 4.45KK13 pKa = 11.3AADD16 pKa = 4.28CVDD19 pKa = 3.73VCPVNCIEE27 pKa = 4.92EE28 pKa = 5.26GPDD31 pKa = 3.15QYY33 pKa = 11.35FIDD36 pKa = 4.46AEE38 pKa = 4.34VCIDD42 pKa = 4.49CGACEE47 pKa = 3.98AACPVSAISWEE58 pKa = 4.04DD59 pKa = 4.1DD60 pKa = 3.42LRR62 pKa = 11.84DD63 pKa = 3.67DD64 pKa = 4.41QKK66 pKa = 11.6IFIEE70 pKa = 4.29KK71 pKa = 9.88AAEE74 pKa = 3.93FFKK77 pKa = 11.26NRR79 pKa = 3.66

Molecular weight: 8.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U2LA52|A0A0U2LA52_9BACL Aminopeptidase OS=Paenibacillus sp. 32O-W OX=1695218 GN=IJ21_20450 PE=3 SV=1
MM1 pKa = 7.42RR2 pKa = 11.84TPGALGARR10 pKa = 11.84QRR12 pKa = 11.84RR13 pKa = 11.84GGGAFAHH20 pKa = 6.14LRR22 pKa = 11.84GARR25 pKa = 11.84GPTAAQGRR33 pKa = 11.84GVRR36 pKa = 11.84APQVRR41 pKa = 11.84YY42 pKa = 9.7GLARR46 pKa = 11.84VRR48 pKa = 11.84GRR50 pKa = 11.84AFAHH54 pKa = 5.98PRR56 pKa = 11.84GARR59 pKa = 11.84GPAAARR65 pKa = 11.84GRR67 pKa = 11.84GVCAPQVRR75 pKa = 11.84YY76 pKa = 10.09GLARR80 pKa = 11.84GAGGGRR86 pKa = 11.84LRR88 pKa = 11.84TPGALGAWQRR98 pKa = 11.84RR99 pKa = 11.84RR100 pKa = 11.84GRR102 pKa = 11.84AFAHH106 pKa = 6.17LRR108 pKa = 11.84GAMGSPASGGGRR120 pKa = 11.84SRR122 pKa = 11.84TSGALGARR130 pKa = 11.84QRR132 pKa = 11.84RR133 pKa = 11.84RR134 pKa = 11.84GRR136 pKa = 11.84AFAHH140 pKa = 6.06PRR142 pKa = 11.84GARR145 pKa = 11.84GPAAAQGAGVRR156 pKa = 11.84APQGRR161 pKa = 3.49

Molecular weight: 16.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4738

0

4738

1543623

29

2771

325.8

36.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.524 ± 0.049

0.776 ± 0.012

5.25 ± 0.026

6.872 ± 0.04

4.053 ± 0.025

7.905 ± 0.03

1.995 ± 0.017

6.133 ± 0.031

4.705 ± 0.031

9.995 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.743 ± 0.015

3.287 ± 0.023

4.474 ± 0.022

3.326 ± 0.022

6.47 ± 0.043

5.552 ± 0.026

4.998 ± 0.023

7.168 ± 0.033

1.388 ± 0.016

3.389 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski