Amino acid dipepetide frequency for Paenibacillus sp. 32O-W

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.274AlaAla: 12.274 ± 0.151	0.836AlaCys: 0.836 ± 0.025	5.491AlaAsp: 5.491 ± 0.076	7.046AlaGlu: 7.046 ± 0.082	3.662AlaPhe: 3.662 ± 0.058	8.577AlaGly: 8.577 ± 0.097	1.581AlaHis: 1.581 ± 0.039	5.463AlaIle: 5.463 ± 0.062	4.178AlaLys: 4.178 ± 0.063	9.605AlaLeu: 9.605 ± 0.108	2.509AlaMet: 2.509 ± 0.04	2.676AlaAsn: 2.676 ± 0.045	3.231AlaPro: 3.231 ± 0.053	2.63AlaGln: 2.63 ± 0.048	4.92AlaArg: 4.92 ± 0.069	5.194AlaSer: 5.194 ± 0.065	3.601AlaThr: 3.601 ± 0.053	7.83AlaVal: 7.83 ± 0.078	1.088AlaTrp: 1.088 ± 0.03	2.847AlaTyr: 2.847 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	0.704CysAla: 0.704 ± 0.023	0.124CysCys: 0.124 ± 0.01	0.4CysAsp: 0.4 ± 0.016	0.472CysGlu: 0.472 ± 0.021	0.303CysPhe: 0.303 ± 0.015	0.918CysGly: 0.918 ± 0.026	0.179CysHis: 0.179 ± 0.013	0.424CysIle: 0.424 ± 0.016	0.277CysLys: 0.277 ± 0.014	0.68CysLeu: 0.68 ± 0.023	0.196CysMet: 0.196 ± 0.011	0.221CysAsn: 0.221 ± 0.013	0.378CysPro: 0.378 ± 0.016	0.166CysGln: 0.166 ± 0.012	0.586CysArg: 0.586 ± 0.022	0.522CysSer: 0.522 ± 0.017	0.378CysThr: 0.378 ± 0.017	0.488CysVal: 0.488 ± 0.022	0.087CysTrp: 0.087 ± 0.008	0.251CysTyr: 0.251 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	4.771AspAla: 4.771 ± 0.066	0.381AspCys: 0.381 ± 0.017	2.69AspAsp: 2.69 ± 0.05	4.037AspGlu: 4.037 ± 0.056	1.915AspPhe: 1.915 ± 0.036	4.893AspGly: 4.893 ± 0.086	0.99AspHis: 0.99 ± 0.024	3.416AspIle: 3.416 ± 0.046	2.447AspLys: 2.447 ± 0.041	4.607AspLeu: 4.607 ± 0.053	1.376AspMet: 1.376 ± 0.029	1.606AspAsn: 1.606 ± 0.033	2.683AspPro: 2.683 ± 0.048	1.461AspGln: 1.461 ± 0.03	3.765AspArg: 3.765 ± 0.057	2.502AspSer: 2.502 ± 0.045	2.345AspThr: 2.345 ± 0.038	3.656AspVal: 3.656 ± 0.054	0.932AspTrp: 0.932 ± 0.025	2.025AspTyr: 2.025 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	7.279GluAla: 7.279 ± 0.083	0.396GluCys: 0.396 ± 0.017	2.887GluAsp: 2.887 ± 0.048	5.429GluGlu: 5.429 ± 0.075	1.942GluPhe: 1.942 ± 0.042	4.532GluGly: 4.532 ± 0.054	1.338GluHis: 1.338 ± 0.029	3.939GluIle: 3.939 ± 0.054	3.608GluLys: 3.608 ± 0.055	7.307GluLeu: 7.307 ± 0.089	2.003GluMet: 2.003 ± 0.036	2.129GluAsn: 2.129 ± 0.042	2.796GluPro: 2.796 ± 0.046	3.229GluGln: 3.229 ± 0.05	5.748GluArg: 5.748 ± 0.069	3.262GluSer: 3.262 ± 0.047	3.816GluThr: 3.816 ± 0.051	4.007GluVal: 4.007 ± 0.055	1.138GluTrp: 1.138 ± 0.029	1.935GluTyr: 1.935 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	3.772PheAla: 3.772 ± 0.056	0.319PheCys: 0.319 ± 0.015	2.335PheAsp: 2.335 ± 0.039	2.387PheGlu: 2.387 ± 0.036	1.802PhePhe: 1.802 ± 0.048	3.481PheGly: 3.481 ± 0.057	0.883PheHis: 0.883 ± 0.03	2.476PheIle: 2.476 ± 0.04	1.653PheLys: 1.653 ± 0.035	3.737PheLeu: 3.737 ± 0.067	1.083PheMet: 1.083 ± 0.029	1.327PheAsn: 1.327 ± 0.033	1.691PhePro: 1.691 ± 0.038	1.237PheGln: 1.237 ± 0.032	2.692PheArg: 2.692 ± 0.044	2.347PheSer: 2.347 ± 0.043	2.063PheThr: 2.063 ± 0.039	3.231PheVal: 3.231 ± 0.055	0.58PheTrp: 0.58 ± 0.021	1.43PheTyr: 1.43 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	6.733GlyAla: 6.733 ± 0.088	0.801GlyCys: 0.801 ± 0.026	3.883GlyAsp: 3.883 ± 0.055	5.215GlyGlu: 5.215 ± 0.068	3.303GlyPhe: 3.303 ± 0.045	6.428GlyGly: 6.428 ± 0.077	1.514GlyHis: 1.514 ± 0.031	5.534GlyIle: 5.534 ± 0.075	4.424GlyLys: 4.424 ± 0.056	7.583GlyLeu: 7.583 ± 0.077	2.523GlyMet: 2.523 ± 0.04	2.505GlyAsn: 2.505 ± 0.046	2.388GlyPro: 2.388 ± 0.043	2.593GlyGln: 2.593 ± 0.047	4.95GlyArg: 4.95 ± 0.072	4.589GlySer: 4.589 ± 0.059	4.474GlyThr: 4.474 ± 0.06	5.374GlyVal: 5.374 ± 0.072	1.272GlyTrp: 1.272 ± 0.031	2.959GlyTyr: 2.959 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	1.702HisAla: 1.702 ± 0.037	0.197HisCys: 0.197 ± 0.011	1.061HisAsp: 1.061 ± 0.027	1.19HisGlu: 1.19 ± 0.029	0.952HisPhe: 0.952 ± 0.022	1.476HisGly: 1.476 ± 0.035	0.557HisHis: 0.557 ± 0.02	1.257HisIle: 1.257 ± 0.027	0.676HisLys: 0.676 ± 0.018	1.988HisLeu: 1.988 ± 0.037	0.494HisMet: 0.494 ± 0.017	0.614HisAsn: 0.614 ± 0.028	1.36HisPro: 1.36 ± 0.032	0.615HisGln: 0.615 ± 0.019	1.299HisArg: 1.299 ± 0.032	0.983HisSer: 0.983 ± 0.025	0.985HisThr: 0.985 ± 0.027	1.362HisVal: 1.362 ± 0.029	0.308HisTrp: 0.308 ± 0.014	0.874HisTyr: 0.874 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	6.385IleAla: 6.385 ± 0.066	0.555IleCys: 0.555 ± 0.021	3.708IleAsp: 3.708 ± 0.057	4.105IleGlu: 4.105 ± 0.054	2.117IlePhe: 2.117 ± 0.039	5.602IleGly: 5.602 ± 0.074	1.192IleHis: 1.192 ± 0.027	3.345IleIle: 3.345 ± 0.051	2.232IleLys: 2.232 ± 0.044	4.754IleLeu: 4.754 ± 0.059	1.458IleMet: 1.458 ± 0.03	1.804IleAsn: 1.804 ± 0.035	3.001IlePro: 3.001 ± 0.047	1.861IleGln: 1.861 ± 0.035	4.774IleArg: 4.774 ± 0.088	3.369IleSer: 3.369 ± 0.056	2.905IleThr: 2.905 ± 0.048	5.524IleVal: 5.524 ± 0.055	0.761IleTrp: 0.761 ± 0.025	1.881IleTyr: 1.881 ± 0.037	0.0IleXaa: 0.0 ± 0.0
Lys	4.224LysAla: 4.224 ± 0.064	0.217LysCys: 0.217 ± 0.014	2.344LysAsp: 2.344 ± 0.046	3.779LysGlu: 3.779 ± 0.056	1.34LysPhe: 1.34 ± 0.03	3.286LysGly: 3.286 ± 0.049	0.967LysHis: 0.967 ± 0.025	2.632LysIle: 2.632 ± 0.046	2.482LysLys: 2.482 ± 0.049	4.966LysLeu: 4.966 ± 0.072	1.329LysMet: 1.329 ± 0.033	1.534LysAsn: 1.534 ± 0.035	2.345LysPro: 2.345 ± 0.037	1.868LysGln: 1.868 ± 0.033	3.364LysArg: 3.364 ± 0.046	2.467LysSer: 2.467 ± 0.045	2.65LysThr: 2.65 ± 0.041	3.062LysVal: 3.062 ± 0.049	0.651LysTrp: 0.651 ± 0.024	1.542LysTyr: 1.542 ± 0.03	0.0LysXaa: 0.0 ± 0.0
Leu	9.872LeuAla: 9.872 ± 0.113	0.765LeuCys: 0.765 ± 0.023	5.147LeuAsp: 5.147 ± 0.062	6.234LeuGlu: 6.234 ± 0.066	4.635LeuPhe: 4.635 ± 0.073	6.73LeuGly: 6.73 ± 0.073	2.025LeuHis: 2.025 ± 0.04	5.882LeuIle: 5.882 ± 0.075	4.8LeuLys: 4.8 ± 0.059	11.141LeuLeu: 11.141 ± 0.14	2.488LeuMet: 2.488 ± 0.043	3.387LeuAsn: 3.387 ± 0.041	4.859LeuPro: 4.859 ± 0.061	3.65LeuGln: 3.65 ± 0.053	6.117LeuArg: 6.117 ± 0.067	6.158LeuSer: 6.158 ± 0.068	5.341LeuThr: 5.341 ± 0.061	6.375LeuVal: 6.375 ± 0.08	1.133LeuTrp: 1.133 ± 0.029	3.212LeuTyr: 3.212 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	2.571MetAla: 2.571 ± 0.041	0.152MetCys: 0.152 ± 0.01	1.322MetAsp: 1.322 ± 0.033	1.812MetGlu: 1.812 ± 0.034	0.994MetPhe: 0.994 ± 0.028	1.598MetGly: 1.598 ± 0.033	0.406MetHis: 0.406 ± 0.015	1.783MetIle: 1.783 ± 0.034	1.877MetLys: 1.877 ± 0.039	2.948MetLeu: 2.948 ± 0.047	0.869MetMet: 0.869 ± 0.028	1.315MetAsn: 1.315 ± 0.033	1.294MetPro: 1.294 ± 0.027	0.862MetGln: 0.862 ± 0.025	1.677MetArg: 1.677 ± 0.032	1.715MetSer: 1.715 ± 0.035	1.704MetThr: 1.704 ± 0.033	1.55MetVal: 1.55 ± 0.035	0.266MetTrp: 0.266 ± 0.014	0.712MetTyr: 0.712 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	2.871AsnAla: 2.871 ± 0.049	0.238AsnCys: 0.238 ± 0.011	1.637AsnAsp: 1.637 ± 0.038	2.254AsnGlu: 2.254 ± 0.044	1.159AsnPhe: 1.159 ± 0.027	3.198AsnGly: 3.198 ± 0.052	0.666AsnHis: 0.666 ± 0.018	2.004AsnIle: 2.004 ± 0.043	1.507AsnLys: 1.507 ± 0.039	2.755AsnLeu: 2.755 ± 0.044	0.909AsnMet: 0.909 ± 0.028	1.09AsnAsn: 1.09 ± 0.03	1.929AsnPro: 1.929 ± 0.036	1.003AsnGln: 1.003 ± 0.028	2.388AsnArg: 2.388 ± 0.034	1.543AsnSer: 1.543 ± 0.036	1.603AsnThr: 1.603 ± 0.036	2.511AsnVal: 2.511 ± 0.043	0.465AsnTrp: 0.465 ± 0.022	1.139AsnTyr: 1.139 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	4.269ProAla: 4.269 ± 0.059	0.288ProCys: 0.288 ± 0.014	3.294ProAsp: 3.294 ± 0.048	3.926ProGlu: 3.926 ± 0.052	2.106ProPhe: 2.106 ± 0.037	3.648ProGly: 3.648 ± 0.053	1.049ProHis: 1.049 ± 0.025	2.418ProIle: 2.418 ± 0.037	1.752ProLys: 1.752 ± 0.032	4.39ProLeu: 4.39 ± 0.057	0.961ProMet: 0.961 ± 0.026	1.507ProAsn: 1.507 ± 0.035	1.72ProPro: 1.72 ± 0.041	1.37ProGln: 1.37 ± 0.034	1.864ProArg: 1.864 ± 0.036	2.492ProSer: 2.492 ± 0.043	1.758ProThr: 1.758 ± 0.034	3.631ProVal: 3.631 ± 0.046	0.612ProTrp: 0.612 ± 0.022	1.687ProTyr: 1.687 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	3.249GlnAla: 3.249 ± 0.046	0.194GlnCys: 0.194 ± 0.011	1.447GlnAsp: 1.447 ± 0.027	2.098GlnGlu: 2.098 ± 0.044	1.368GlnPhe: 1.368 ± 0.033	2.246GlnGly: 2.246 ± 0.038	0.672GlnHis: 0.672 ± 0.019	2.122GlnIle: 2.122 ± 0.042	1.532GlnLys: 1.532 ± 0.031	3.598GlnLeu: 3.598 ± 0.057	0.989GlnMet: 0.989 ± 0.025	1.143GlnAsn: 1.143 ± 0.029	1.534GlnPro: 1.534 ± 0.03	1.501GlnGln: 1.501 ± 0.035	2.013GlnArg: 2.013 ± 0.046	1.917GlnSer: 1.917 ± 0.04	1.794GlnThr: 1.794 ± 0.036	2.081GlnVal: 2.081 ± 0.038	0.556GlnTrp: 0.556 ± 0.018	1.203GlnTyr: 1.203 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	4.487ArgAla: 4.487 ± 0.068	0.518ArgCys: 0.518 ± 0.02	3.15ArgAsp: 3.15 ± 0.052	4.957ArgGlu: 4.957 ± 0.062	2.841ArgPhe: 2.841 ± 0.046	3.67ArgGly: 3.67 ± 0.047	1.535ArgHis: 1.535 ± 0.032	4.485ArgIle: 4.485 ± 0.063	3.572ArgLys: 3.572 ± 0.046	7.162ArgLeu: 7.162 ± 0.089	2.097ArgMet: 2.097 ± 0.038	2.35ArgAsn: 2.35 ± 0.036	2.684ArgPro: 2.684 ± 0.049	2.632ArgGln: 2.632 ± 0.046	4.385ArgArg: 4.385 ± 0.069	3.752ArgSer: 3.752 ± 0.053	3.432ArgThr: 3.432 ± 0.047	3.637ArgVal: 3.637 ± 0.069	0.946ArgTrp: 0.946 ± 0.027	2.404ArgTyr: 2.404 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	5.168SerAla: 5.168 ± 0.062	0.424SerCys: 0.424 ± 0.017	2.822SerAsp: 2.822 ± 0.045	3.239SerGlu: 3.239 ± 0.051	2.769SerPhe: 2.769 ± 0.055	5.639SerGly: 5.639 ± 0.074	1.066SerHis: 1.066 ± 0.025	3.382SerIle: 3.382 ± 0.053	2.348SerLys: 2.348 ± 0.042	5.583SerLeu: 5.583 ± 0.076	1.504SerMet: 1.504 ± 0.031	1.642SerAsn: 1.642 ± 0.035	2.563SerPro: 2.563 ± 0.042	1.52SerGln: 1.52 ± 0.032	3.481SerArg: 3.481 ± 0.051	3.187SerSer: 3.187 ± 0.056	2.304SerThr: 2.304 ± 0.037	4.267SerVal: 4.267 ± 0.065	0.767SerTrp: 0.767 ± 0.021	1.84SerTyr: 1.84 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	4.938ThrAla: 4.938 ± 0.061	0.328ThrCys: 0.328 ± 0.017	2.784ThrAsp: 2.784 ± 0.042	3.025ThrGlu: 3.025 ± 0.054	2.233ThrPhe: 2.233 ± 0.042	4.644ThrGly: 4.644 ± 0.05	0.911ThrHis: 0.911 ± 0.033	3.389ThrIle: 3.389 ± 0.049	1.97ThrLys: 1.97 ± 0.041	5.055ThrLeu: 5.055 ± 0.056	1.335ThrMet: 1.335 ± 0.029	1.631ThrAsn: 1.631 ± 0.037	2.459ThrPro: 2.459 ± 0.04	1.2ThrGln: 1.2 ± 0.029	2.277ThrArg: 2.277 ± 0.036	2.563ThrSer: 2.563 ± 0.04	2.329ThrThr: 2.329 ± 0.034	4.629ThrVal: 4.629 ± 0.056	0.613ThrTrp: 0.613 ± 0.023	1.665ThrTyr: 1.665 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	5.996ValAla: 5.996 ± 0.075	0.652ValCys: 0.652 ± 0.019	3.484ValAsp: 3.484 ± 0.058	4.358ValGlu: 4.358 ± 0.058	2.917ValPhe: 2.917 ± 0.05	4.762ValGly: 4.762 ± 0.086	1.43ValHis: 1.43 ± 0.03	4.568ValIle: 4.568 ± 0.062	3.603ValLys: 3.603 ± 0.05	7.262ValLeu: 7.262 ± 0.071	1.958ValMet: 1.958 ± 0.037	2.475ValAsn: 2.475 ± 0.044	3.678ValPro: 3.678 ± 0.05	2.31ValGln: 2.31 ± 0.041	4.938ValArg: 4.938 ± 0.065	4.4ValSer: 4.4 ± 0.068	4.087ValThr: 4.087 ± 0.065	5.285ValVal: 5.285 ± 0.066	0.982ValTrp: 0.982 ± 0.027	2.535ValTyr: 2.535 ± 0.046	0.0ValXaa: 0.0 ± 0.0
Trp	0.967TrpAla: 0.967 ± 0.026	0.099TrpCys: 0.099 ± 0.008	0.737TrpAsp: 0.737 ± 0.023	0.816TrpGlu: 0.816 ± 0.022	0.624TrpPhe: 0.624 ± 0.021	0.868TrpGly: 0.868 ± 0.026	0.312TrpHis: 0.312 ± 0.016	0.927TrpIle: 0.927 ± 0.024	0.748TrpLys: 0.748 ± 0.022	1.618TrpLeu: 1.618 ± 0.038	0.468TrpMet: 0.468 ± 0.02	0.674TrpAsn: 0.674 ± 0.022	0.505TrpPro: 0.505 ± 0.019	0.538TrpGln: 0.538 ± 0.02	0.874TrpArg: 0.874 ± 0.025	0.798TrpSer: 0.798 ± 0.023	0.801TrpThr: 0.801 ± 0.028	0.84TrpVal: 0.84 ± 0.023	0.216TrpTrp: 0.216 ± 0.014	0.449TrpTyr: 0.449 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.006TyrAla: 3.006 ± 0.048	0.272TyrCys: 0.272 ± 0.013	1.877TyrAsp: 1.877 ± 0.039	2.341TyrGlu: 2.341 ± 0.045	1.545TyrPhe: 1.545 ± 0.034	2.848TyrGly: 2.848 ± 0.049	0.678TyrHis: 0.678 ± 0.022	1.886TyrIle: 1.886 ± 0.035	1.364TyrLys: 1.364 ± 0.031	3.21TyrLeu: 3.21 ± 0.057	0.879TyrMet: 0.879 ± 0.024	1.242TyrAsn: 1.242 ± 0.031	1.643TyrPro: 1.643 ± 0.032	1.011TyrGln: 1.011 ± 0.026	2.584TyrArg: 2.584 ± 0.045	1.756TyrSer: 1.756 ± 0.036	1.607TyrThr: 1.607 ± 0.034	2.339TyrVal: 2.339 ± 0.04	0.503TyrTrp: 0.503 ± 0.018	1.3TyrTyr: 1.3 ± 0.031	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4738 proteins (1543623 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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