Rhodococcus sp. EPR-157

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus; unclassified Rhodococcus

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5176 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A164QSB1|A0A164QSB1_9NOCA Single-stranded DNA-binding protein OS=Rhodococcus sp. EPR-157 OX=1813677 GN=A2J03_00050 PE=4 SV=1
MM1 pKa = 7.77LSTNLIRR8 pKa = 11.84NSLAVSAAIATIALLAPGAAVAQPSTGSADD38 pKa = 3.68DD39 pKa = 4.43VISGGSGILDD49 pKa = 3.81GGSSVLDD56 pKa = 3.45GDD58 pKa = 4.26IADD61 pKa = 4.21GAAGIVDD68 pKa = 4.03SGSSILNSLPGTGSFAPRR86 pKa = 11.84QLCNQDD92 pKa = 3.12TVAGGPGVTQTNHH105 pKa = 6.65DD106 pKa = 4.34LGRR109 pKa = 11.84PGPNSFVLRR118 pKa = 11.84YY119 pKa = 6.93EE120 pKa = 4.37TINIPDD126 pKa = 4.66IIDD129 pKa = 3.26VFYY132 pKa = 10.87QGALIYY138 pKa = 9.53STGYY142 pKa = 10.52VGDD145 pKa = 4.7NINEE149 pKa = 4.25GTGSVVVNVPPGTDD163 pKa = 2.68TSVLVKK169 pKa = 9.52VTGPGGTDD177 pKa = 2.49WDD179 pKa = 4.35YY180 pKa = 11.28TVGCPIAA187 pKa = 5.64

Molecular weight: 18.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A164QYJ1|A0A164QYJ1_9NOCA Hydroxymethylpyrimidine kinase OS=Rhodococcus sp. EPR-157 OX=1813677 GN=A2J03_20675 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 10.01GRR42 pKa = 11.84KK43 pKa = 8.43EE44 pKa = 3.47LTAA47 pKa = 4.94

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5176

0

5176

1620607

37

5804

313.1

33.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.352 ± 0.043

0.716 ± 0.009

6.434 ± 0.029

5.473 ± 0.028

3.12 ± 0.022

8.724 ± 0.031

2.117 ± 0.016

4.59 ± 0.021

2.282 ± 0.027

9.879 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.984 ± 0.015

2.195 ± 0.019

5.26 ± 0.026

2.755 ± 0.014

6.86 ± 0.033

6.405 ± 0.024

6.325 ± 0.023

9.046 ± 0.033

1.419 ± 0.013

2.065 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski