Amino acid dipepetide frequency for Rhodococcus sp. EPR-157

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.487AlaAla: 17.487 ± 0.155
0.872AlaCys: 0.872 ± 0.025
7.872AlaAsp: 7.872 ± 0.073
7.315AlaGlu: 7.315 ± 0.08
3.554AlaPhe: 3.554 ± 0.048
10.659AlaGly: 10.659 ± 0.091
2.487AlaHis: 2.487 ± 0.042
5.383AlaIle: 5.383 ± 0.063
2.84AlaLys: 2.84 ± 0.054
13.06AlaLeu: 13.06 ± 0.115
2.593AlaMet: 2.593 ± 0.042
2.297AlaAsn: 2.297 ± 0.041
5.592AlaPro: 5.592 ± 0.069
3.65AlaGln: 3.65 ± 0.046
8.028AlaArg: 8.028 ± 0.076
7.307AlaSer: 7.307 ± 0.069
7.125AlaThr: 7.125 ± 0.073
11.712AlaVal: 11.712 ± 0.094
1.522AlaTrp: 1.522 ± 0.032
2.162AlaTyr: 2.162 ± 0.033
0.0AlaXaa: 0.0 ± 0.0
Cys
0.958CysAla: 0.958 ± 0.025
0.08CysCys: 0.08 ± 0.007
0.451CysAsp: 0.451 ± 0.017
0.383CysGlu: 0.383 ± 0.015
0.21CysPhe: 0.21 ± 0.01
0.803CysGly: 0.803 ± 0.022
0.134CysHis: 0.134 ± 0.01
0.301CysIle: 0.301 ± 0.014
0.106CysLys: 0.106 ± 0.007
0.562CysLeu: 0.562 ± 0.019
0.125CysMet: 0.125 ± 0.009
0.155CysAsn: 0.155 ± 0.009
0.399CysPro: 0.399 ± 0.018
0.155CysGln: 0.155 ± 0.01
0.495CysArg: 0.495 ± 0.017
0.52CysSer: 0.52 ± 0.018
0.476CysThr: 0.476 ± 0.018
0.605CysVal: 0.605 ± 0.021
0.098CysTrp: 0.098 ± 0.008
0.149CysTyr: 0.149 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
8.064AspAla: 8.064 ± 0.076
0.383AspCys: 0.383 ± 0.016
4.058AspAsp: 4.058 ± 0.059
4.024AspGlu: 4.024 ± 0.057
1.741AspPhe: 1.741 ± 0.034
6.038AspGly: 6.038 ± 0.076
1.351AspHis: 1.351 ± 0.031
2.968AspIle: 2.968 ± 0.043
1.395AspLys: 1.395 ± 0.032
6.269AspLeu: 6.269 ± 0.072
0.989AspMet: 0.989 ± 0.028
1.3AspAsn: 1.3 ± 0.03
4.086AspPro: 4.086 ± 0.046
1.61AspGln: 1.61 ± 0.032
4.658AspArg: 4.658 ± 0.059
3.817AspSer: 3.817 ± 0.051
3.611AspThr: 3.611 ± 0.048
5.695AspVal: 5.695 ± 0.067
0.902AspTrp: 0.902 ± 0.02
1.377AspTyr: 1.377 ± 0.033
0.0AspXaa: 0.0 ± 0.0
Glu
5.795GluAla: 5.795 ± 0.072
0.383GluCys: 0.383 ± 0.016
2.751GluAsp: 2.751 ± 0.043
2.719GluGlu: 2.719 ± 0.049
1.967GluPhe: 1.967 ± 0.034
3.544GluGly: 3.544 ± 0.054
1.56GluHis: 1.56 ± 0.034
2.748GluIle: 2.748 ± 0.04
1.617GluLys: 1.617 ± 0.037
6.696GluLeu: 6.696 ± 0.07
1.105GluMet: 1.105 ± 0.024
1.467GluAsn: 1.467 ± 0.029
2.892GluPro: 2.892 ± 0.044
2.197GluGln: 2.197 ± 0.04
4.328GluArg: 4.328 ± 0.054
3.65GluSer: 3.65 ± 0.049
2.898GluThr: 2.898 ± 0.041
4.408GluVal: 4.408 ± 0.057
0.84GluTrp: 0.84 ± 0.023
1.167GluTyr: 1.167 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
4.109PheAla: 4.109 ± 0.05
0.27PheCys: 0.27 ± 0.014
2.694PheAsp: 2.694 ± 0.05
1.798PheGlu: 1.798 ± 0.037
1.051PhePhe: 1.051 ± 0.029
3.576PheGly: 3.576 ± 0.052
0.656PheHis: 0.656 ± 0.02
1.026PheIle: 1.026 ± 0.031
0.518PheLys: 0.518 ± 0.022
2.65PheLeu: 2.65 ± 0.05
0.503PheMet: 0.503 ± 0.018
0.584PheAsn: 0.584 ± 0.018
1.352PhePro: 1.352 ± 0.034
0.7PheGln: 0.7 ± 0.021
1.684PheArg: 1.684 ± 0.03
1.815PheSer: 1.815 ± 0.03
2.074PheThr: 2.074 ± 0.036
3.0PheVal: 3.0 ± 0.046
0.425PheTrp: 0.425 ± 0.018
0.713PheTyr: 0.713 ± 0.022
0.0PheXaa: 0.0 ± 0.0
Gly
9.503GlyAla: 9.503 ± 0.087
0.736GlyCys: 0.736 ± 0.021
4.837GlyAsp: 4.837 ± 0.061
4.374GlyGlu: 4.374 ± 0.052
3.151GlyPhe: 3.151 ± 0.048
7.494GlyGly: 7.494 ± 0.087
1.969GlyHis: 1.969 ± 0.039
4.649GlyIle: 4.649 ± 0.056
2.463GlyLys: 2.463 ± 0.039
8.545GlyLeu: 8.545 ± 0.075
1.942GlyMet: 1.942 ± 0.034
1.978GlyAsn: 1.978 ± 0.041
4.048GlyPro: 4.048 ± 0.047
2.499GlyGln: 2.499 ± 0.045
5.977GlyArg: 5.977 ± 0.062
5.999GlySer: 5.999 ± 0.065
5.62GlyThr: 5.62 ± 0.054
7.715GlyVal: 7.715 ± 0.068
1.503GlyTrp: 1.503 ± 0.036
2.242GlyTyr: 2.242 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
2.389HisAla: 2.389 ± 0.038
0.193HisCys: 0.193 ± 0.013
1.319HisAsp: 1.319 ± 0.032
1.106HisGlu: 1.106 ± 0.024
0.645HisPhe: 0.645 ± 0.019
2.026HisGly: 2.026 ± 0.04
0.658HisHis: 0.658 ± 0.023
0.879HisIle: 0.879 ± 0.024
0.37HisLys: 0.37 ± 0.014
2.063HisLeu: 2.063 ± 0.042
0.351HisMet: 0.351 ± 0.015
0.428HisAsn: 0.428 ± 0.016
1.482HisPro: 1.482 ± 0.031
0.574HisGln: 0.574 ± 0.019
1.765HisArg: 1.765 ± 0.038
1.285HisSer: 1.285 ± 0.032
1.171HisThr: 1.171 ± 0.025
1.704HisVal: 1.704 ± 0.033
0.298HisTrp: 0.298 ± 0.013
0.463HisTyr: 0.463 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
6.801IleAla: 6.801 ± 0.073
0.36IleCys: 0.36 ± 0.014
3.877IleAsp: 3.877 ± 0.052
2.994IleGlu: 2.994 ± 0.043
1.116IlePhe: 1.116 ± 0.029
4.797IleGly: 4.797 ± 0.056
0.802IleHis: 0.802 ± 0.023
1.565IleIle: 1.565 ± 0.039
0.807IleLys: 0.807 ± 0.024
3.766IleLeu: 3.766 ± 0.052
0.678IleMet: 0.678 ± 0.023
0.974IleAsn: 0.974 ± 0.027
2.478IlePro: 2.478 ± 0.039
0.906IleGln: 0.906 ± 0.027
2.657IleArg: 2.657 ± 0.041
2.651IleSer: 2.651 ± 0.04
2.879IleThr: 2.879 ± 0.044
4.539IleVal: 4.539 ± 0.05
0.466IleTrp: 0.466 ± 0.018
0.784IleTyr: 0.784 ± 0.023
0.0IleXaa: 0.0 ± 0.0
Lys
2.714LysAla: 2.714 ± 0.051
0.108LysCys: 0.108 ± 0.008
1.223LysAsp: 1.223 ± 0.03
1.02LysGlu: 1.02 ± 0.029
0.772LysPhe: 0.772 ± 0.024
1.587LysGly: 1.587 ± 0.033
0.48LysHis: 0.48 ± 0.017
1.256LysIle: 1.256 ± 0.031
0.889LysLys: 0.889 ± 0.034
2.213LysLeu: 2.213 ± 0.037
0.522LysMet: 0.522 ± 0.018
0.624LysAsn: 0.624 ± 0.023
1.373LysPro: 1.373 ± 0.033
0.718LysGln: 0.718 ± 0.019
1.531LysArg: 1.531 ± 0.034
1.506LysSer: 1.506 ± 0.035
1.432LysThr: 1.432 ± 0.032
1.936LysVal: 1.936 ± 0.041
0.329LysTrp: 0.329 ± 0.016
0.589LysTyr: 0.589 ± 0.02
0.0LysXaa: 0.0 ± 0.0
Leu
13.333LeuAla: 13.333 ± 0.094
0.732LeuCys: 0.732 ± 0.022
7.296LeuAsp: 7.296 ± 0.073
4.807LeuGlu: 4.807 ± 0.06
2.912LeuPhe: 2.912 ± 0.046
9.172LeuGly: 9.172 ± 0.085
1.988LeuHis: 1.988 ± 0.037
4.204LeuIle: 4.204 ± 0.063
1.789LeuLys: 1.789 ± 0.037
9.61LeuLeu: 9.61 ± 0.107
1.723LeuMet: 1.723 ± 0.034
1.899LeuAsn: 1.899 ± 0.035
5.363LeuPro: 5.363 ± 0.059
2.4LeuGln: 2.4 ± 0.038
7.067LeuArg: 7.067 ± 0.077
6.138LeuSer: 6.138 ± 0.064
6.351LeuThr: 6.351 ± 0.067
9.119LeuVal: 9.119 ± 0.09
1.159LeuTrp: 1.159 ± 0.027
1.731LeuTyr: 1.731 ± 0.032
0.0LeuXaa: 0.0 ± 0.0
Met
2.245MetAla: 2.245 ± 0.037
0.144MetCys: 0.144 ± 0.01
0.931MetAsp: 0.931 ± 0.022
0.722MetGlu: 0.722 ± 0.021
0.661MetPhe: 0.661 ± 0.021
1.403MetGly: 1.403 ± 0.032
0.433MetHis: 0.433 ± 0.017
1.04MetIle: 1.04 ± 0.027
0.529MetLys: 0.529 ± 0.017
1.994MetLeu: 1.994 ± 0.032
0.405MetMet: 0.405 ± 0.015
0.582MetAsn: 0.582 ± 0.018
1.11MetPro: 1.11 ± 0.027
0.56MetGln: 0.56 ± 0.02
1.353MetArg: 1.353 ± 0.029
1.694MetSer: 1.694 ± 0.035
1.855MetThr: 1.855 ± 0.031
1.567MetVal: 1.567 ± 0.027
0.246MetTrp: 0.246 ± 0.011
0.365MetTyr: 0.365 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
2.545AsnAla: 2.545 ± 0.04
0.154AsnCys: 0.154 ± 0.01
1.27AsnAsp: 1.27 ± 0.03
1.095AsnGlu: 1.095 ± 0.023
0.694AsnPhe: 0.694 ± 0.02
1.983AsnGly: 1.983 ± 0.038
0.431AsnHis: 0.431 ± 0.015
1.03AsnIle: 1.03 ± 0.025
0.513AsnLys: 0.513 ± 0.019
2.075AsnLeu: 2.075 ± 0.036
0.402AsnMet: 0.402 ± 0.015
0.562AsnAsn: 0.562 ± 0.021
1.641AsnPro: 1.641 ± 0.033
0.586AsnGln: 0.586 ± 0.019
1.47AsnArg: 1.47 ± 0.032
1.419AsnSer: 1.419 ± 0.033
1.388AsnThr: 1.388 ± 0.033
1.751AsnVal: 1.751 ± 0.04
0.359AsnTrp: 0.359 ± 0.015
0.587AsnTyr: 0.587 ± 0.02
0.0AsnXaa: 0.0 ± 0.0
Pro
6.089ProAla: 6.089 ± 0.073
0.273ProCys: 0.273 ± 0.015
3.936ProAsp: 3.936 ± 0.053
3.568ProGlu: 3.568 ± 0.054
1.56ProPhe: 1.56 ± 0.029
4.784ProGly: 4.784 ± 0.062
1.087ProHis: 1.087 ± 0.027
2.39ProIle: 2.39 ± 0.039
1.303ProLys: 1.303 ± 0.031
4.607ProLeu: 4.607 ± 0.057
1.048ProMet: 1.048 ± 0.026
1.256ProAsn: 1.256 ± 0.031
2.545ProPro: 2.545 ± 0.053
1.462ProGln: 1.462 ± 0.033
3.04ProArg: 3.04 ± 0.043
3.526ProSer: 3.526 ± 0.056
3.602ProThr: 3.602 ± 0.046
4.724ProVal: 4.724 ± 0.062
0.74ProTrp: 0.74 ± 0.024
1.055ProTyr: 1.055 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
3.042GlnAla: 3.042 ± 0.046
0.183GlnCys: 0.183 ± 0.013
1.269GlnAsp: 1.269 ± 0.029
1.158GlnGlu: 1.158 ± 0.028
0.98GlnPhe: 0.98 ± 0.028
1.923GlnGly: 1.923 ± 0.036
0.621GlnHis: 0.621 ± 0.022
1.519GlnIle: 1.519 ± 0.029
0.679GlnLys: 0.679 ± 0.024
3.236GlnLeu: 3.236 ± 0.051
0.616GlnMet: 0.616 ± 0.021
0.71GlnAsn: 0.71 ± 0.025
1.464GlnPro: 1.464 ± 0.031
1.195GlnGln: 1.195 ± 0.033
2.205GlnArg: 2.205 ± 0.044
1.702GlnSer: 1.702 ± 0.039
1.457GlnThr: 1.457 ± 0.028
2.383GlnVal: 2.383 ± 0.041
0.562GlnTrp: 0.562 ± 0.017
0.644GlnTyr: 0.644 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
7.639ArgAla: 7.639 ± 0.085
0.472ArgCys: 0.472 ± 0.018
3.874ArgAsp: 3.874 ± 0.046
3.923ArgGlu: 3.923 ± 0.054
2.212ArgPhe: 2.212 ± 0.039
4.915ArgGly: 4.915 ± 0.059
1.531ArgHis: 1.531 ± 0.029
3.641ArgIle: 3.641 ± 0.046
1.69ArgLys: 1.69 ± 0.035
6.48ArgLeu: 6.48 ± 0.074
1.709ArgMet: 1.709 ± 0.031
1.605ArgAsn: 1.605 ± 0.028
3.233ArgPro: 3.233 ± 0.046
1.945ArgGln: 1.945 ± 0.041
5.916ArgArg: 5.916 ± 0.075
4.796ArgSer: 4.796 ± 0.066
4.787ArgThr: 4.787 ± 0.054
5.363ArgVal: 5.363 ± 0.056
1.191ArgTrp: 1.191 ± 0.027
1.678ArgTyr: 1.678 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
7.961SerAla: 7.961 ± 0.078
0.411SerCys: 0.411 ± 0.017
4.018SerAsp: 4.018 ± 0.046
3.462SerGlu: 3.462 ± 0.043
1.923SerPhe: 1.923 ± 0.04
6.196SerGly: 6.196 ± 0.066
1.098SerHis: 1.098 ± 0.03
3.157SerIle: 3.157 ± 0.043
1.476SerLys: 1.476 ± 0.031
5.711SerLeu: 5.711 ± 0.064
1.612SerMet: 1.612 ± 0.037
1.452SerAsn: 1.452 ± 0.031
3.212SerPro: 3.212 ± 0.046
1.576SerGln: 1.576 ± 0.031
3.988SerArg: 3.988 ± 0.053
4.341SerSer: 4.341 ± 0.06
4.432SerThr: 4.432 ± 0.053
5.684SerVal: 5.684 ± 0.066
0.957SerTrp: 0.957 ± 0.022
1.379SerTyr: 1.379 ± 0.026
0.0SerXaa: 0.0 ± 0.0
Thr
7.84ThrAla: 7.84 ± 0.075
0.396ThrCys: 0.396 ± 0.015
4.0ThrAsp: 4.0 ± 0.046
3.509ThrGlu: 3.509 ± 0.054
1.959ThrPhe: 1.959 ± 0.036
5.821ThrGly: 5.821 ± 0.059
1.201ThrHis: 1.201 ± 0.025
2.772ThrIle: 2.772 ± 0.042
1.335ThrLys: 1.335 ± 0.032
5.913ThrLeu: 5.913 ± 0.055
1.195ThrMet: 1.195 ± 0.026
1.246ThrAsn: 1.246 ± 0.029
3.77ThrPro: 3.77 ± 0.054
1.506ThrGln: 1.506 ± 0.031
3.733ThrArg: 3.733 ± 0.047
4.08ThrSer: 4.08 ± 0.053
4.31ThrThr: 4.31 ± 0.066
6.501ThrVal: 6.501 ± 0.064
0.848ThrTrp: 0.848 ± 0.027
1.314ThrTyr: 1.314 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
11.244ValAla: 11.244 ± 0.089
0.675ValCys: 0.675 ± 0.02
6.502ValAsp: 6.502 ± 0.067
5.038ValGlu: 5.038 ± 0.055
2.764ValPhe: 2.764 ± 0.042
7.585ValGly: 7.585 ± 0.077
1.984ValHis: 1.984 ± 0.042
3.866ValIle: 3.866 ± 0.055
1.781ValLys: 1.781 ± 0.04
9.617ValLeu: 9.617 ± 0.083
1.662ValMet: 1.662 ± 0.029
1.907ValAsn: 1.907 ± 0.031
4.751ValPro: 4.751 ± 0.061
2.181ValGln: 2.181 ± 0.034
5.954ValArg: 5.954 ± 0.062
5.478ValSer: 5.478 ± 0.057
5.638ValThr: 5.638 ± 0.067
9.261ValVal: 9.261 ± 0.096
1.073ValTrp: 1.073 ± 0.029
1.498ValTyr: 1.498 ± 0.035
0.0ValXaa: 0.0 ± 0.0
Trp
1.495TrpAla: 1.495 ± 0.029
0.144TrpCys: 0.144 ± 0.009
0.731TrpAsp: 0.731 ± 0.024
0.6TrpGlu: 0.6 ± 0.019
0.549TrpPhe: 0.549 ± 0.019
0.984TrpGly: 0.984 ± 0.027
0.324TrpHis: 0.324 ± 0.014
0.747TrpIle: 0.747 ± 0.021
0.368TrpLys: 0.368 ± 0.018
1.524TrpLeu: 1.524 ± 0.035
0.346TrpMet: 0.346 ± 0.013
0.461TrpAsn: 0.461 ± 0.017
0.679TrpPro: 0.679 ± 0.019
0.519TrpGln: 0.519 ± 0.018
1.045TrpArg: 1.045 ± 0.028
0.975TrpSer: 0.975 ± 0.025
0.967TrpThr: 0.967 ± 0.024
1.102TrpVal: 1.102 ± 0.027
0.341TrpTrp: 0.341 ± 0.016
0.288TrpTyr: 0.288 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.265TyrAla: 2.265 ± 0.041
0.197TyrCys: 0.197 ± 0.011
1.428TyrAsp: 1.428 ± 0.031
1.117TyrGlu: 1.117 ± 0.028
0.776TyrPhe: 0.776 ± 0.023
1.953TyrGly: 1.953 ± 0.032
0.375TyrHis: 0.375 ± 0.016
0.756TyrIle: 0.756 ± 0.021
0.354TyrLys: 0.354 ± 0.017
2.203TyrLeu: 2.203 ± 0.038
0.31TyrMet: 0.31 ± 0.014
0.468TyrAsn: 0.468 ± 0.014
1.129TyrPro: 1.129 ± 0.028
0.61TyrGln: 0.61 ± 0.02
1.706TyrArg: 1.706 ± 0.032
1.348TyrSer: 1.348 ± 0.03
1.174TyrThr: 1.174 ± 0.028
1.688TyrVal: 1.688 ± 0.031
0.331TyrTrp: 0.331 ± 0.014
0.462TyrTyr: 0.462 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5176 proteins (1620607 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski