Pigeon adenovirus 2
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 35 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D8QM97|A0A1D8QM97_9ADEN Protein ORF13 OS=Pigeon adenovirus 2 OX=1907767 GN=ORF13 PE=4 SV=1
MM1 pKa = 7.65 LKK3 pKa = 10.1 RR4 pKa = 11.84 RR5 pKa = 11.84 NEE7 pKa = 3.83 LSDD10 pKa = 3.14 DD11 pKa = 4.19 RR12 pKa = 11.84 KK13 pKa = 10.18 RR14 pKa = 11.84 KK15 pKa = 9.36 RR16 pKa = 11.84 PRR18 pKa = 11.84 AVGSSDD24 pKa = 4.68 SINLNYY30 pKa = 9.41 PFWYY34 pKa = 9.82 SAEE37 pKa = 4.14 EE38 pKa = 3.89 NTEE41 pKa = 3.79 IVPPFIDD48 pKa = 4.02 PSGPLYY54 pKa = 11.08 DD55 pKa = 4.43 QDD57 pKa = 3.35 GKK59 pKa = 11.68 LNIRR63 pKa = 11.84 LTSPIALISNGVGLGYY79 pKa = 10.57 DD80 pKa = 3.24 SSLEE84 pKa = 4.05 NVNNRR89 pKa = 11.84 LGVRR93 pKa = 11.84 IDD95 pKa = 3.22 SDD97 pKa = 3.74 GALEE101 pKa = 4.04 VTPDD105 pKa = 3.61 GLNVRR110 pKa = 11.84 VDD112 pKa = 3.86 DD113 pKa = 4.13 EE114 pKa = 4.92 TVGINPSWEE123 pKa = 4.11 LAVQFADD130 pKa = 3.62 EE131 pKa = 4.65 QPFSTSTAGIGLNVDD146 pKa = 3.52 DD147 pKa = 4.99 TLLISSSDD155 pKa = 3.56 SDD157 pKa = 3.71 PSHH160 pKa = 6.89 YY161 pKa = 10.61 EE162 pKa = 3.7 LGVHH166 pKa = 6.93 LNTEE170 pKa = 4.43 GPITADD176 pKa = 3.27 EE177 pKa = 4.35 NGIDD181 pKa = 5.11 LDD183 pKa = 4.42 FNPHH187 pKa = 5.43 TLTVQTNEE195 pKa = 3.79 QNQGILSVFLKK206 pKa = 11.14 ANGGLGATEE215 pKa = 4.03 SGIGVNYY222 pKa = 10.22 DD223 pKa = 3.59 EE224 pKa = 5.45 QSLHH228 pKa = 6.86 LDD230 pKa = 3.38 SGNDD234 pKa = 3.37 LAVRR238 pKa = 11.84 FAPEE242 pKa = 3.6 QPFTVTPQGITLNIDD257 pKa = 3.28 PSSLQVQNGTLSALTSGLGLDD278 pKa = 3.99 IDD280 pKa = 4.54 NNTLQVDD287 pKa = 4.1 TVNNKK292 pKa = 6.99 EE293 pKa = 4.27 TLSVRR298 pKa = 11.84 IAPDD302 pKa = 3.29 NPVEE306 pKa = 4.46 ADD308 pKa = 3.14 SSGIKK313 pKa = 10.23 LLYY316 pKa = 8.76 NTGDD320 pKa = 3.49 FFDD323 pKa = 4.64 NGQSGLSAVTPISYY337 pKa = 10.42 LSPYY341 pKa = 9.7 CYY343 pKa = 10.64 YY344 pKa = 10.63 EE345 pKa = 5.86 AGDD348 pKa = 4.19 SSLTDD353 pKa = 3.16 YY354 pKa = 11.34 SSTVRR359 pKa = 11.84 SSGNTNWPIAAYY371 pKa = 10.26 VKK373 pKa = 10.21 VANCSGVCNGVLNFVLNRR391 pKa = 11.84 NNITFLGTTNDD402 pKa = 3.39 SNNNYY407 pKa = 10.0 VKK409 pKa = 10.57 FCFVLNPGGNLDD421 pKa = 4.61 GIHH424 pKa = 6.73 SYY426 pKa = 8.73 VHH428 pKa = 5.79 NNIYY432 pKa = 10.29 FPAGANTPTFMAPTRR447 pKa = 11.84 TPPPNQTIPANWANWFVAKK466 pKa = 9.62 WVGVRR471 pKa = 11.84 VRR473 pKa = 11.84 FYY475 pKa = 10.93 ATDD478 pKa = 3.19 SSGNQAIEE486 pKa = 4.66 SYY488 pKa = 8.98 VHH490 pKa = 6.43 YY491 pKa = 10.79 YY492 pKa = 8.77 PALVNGSSGYY502 pKa = 9.52 PVIFFVFEE510 pKa = 3.55 IRR512 pKa = 11.84 LPSGQNWYY520 pKa = 8.53 TGSGLSTDD528 pKa = 3.95 YY529 pKa = 11.1 EE530 pKa = 4.32 ILNSGPIPFQYY541 pKa = 10.88 LATKK545 pKa = 10.08 PSYY548 pKa = 9.65 TAVSQQ553 pKa = 3.51
Molecular weight: 59.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.059
IPC2_protein 4.279
IPC_protein 4.279
Toseland 4.075
ProMoST 4.418
Dawson 4.253
Bjellqvist 4.406
Wikipedia 4.164
Rodwell 4.101
Grimsley 3.986
Solomon 4.253
Lehninger 4.202
Nozaki 4.355
DTASelect 4.596
Thurlkill 4.101
EMBOSS 4.177
Sillero 4.393
Patrickios 2.473
IPC_peptide 4.253
IPC2_peptide 4.38
IPC2.peptide.svr19 4.301
Protein with the highest isoelectric point:
>tr|A0A1D8QMA3|A0A1D8QMA3_9ADEN Protein ORF0 OS=Pigeon adenovirus 2 OX=1907767 GN=ORF0 PE=4 SV=1
MM1 pKa = 7.74 SILISPSDD9 pKa = 3.46 NRR11 pKa = 11.84 GWGMRR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 TRR20 pKa = 11.84 SSMRR24 pKa = 11.84 GVGVRR29 pKa = 11.84 RR30 pKa = 11.84 PLTLRR35 pKa = 11.84 RR36 pKa = 11.84 LLGLGTTTSRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 NRR50 pKa = 11.84 RR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 AVRR56 pKa = 11.84 GGASRR61 pKa = 11.84 RR62 pKa = 11.84 TSTSTRR68 pKa = 11.84 IIAVRR73 pKa = 11.84 TRR75 pKa = 11.84 TSRR78 pKa = 11.84 RR79 pKa = 11.84 RR80 pKa = 11.84 RR81 pKa = 11.84 RR82 pKa = 3.32
Molecular weight: 9.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.33
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.618
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.325
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.278
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
35
0
35
12993
40
1368
371.2
42.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.757 ± 0.309
1.793 ± 0.217
5.257 ± 0.22
5.749 ± 0.559
4.526 ± 0.265
5.711 ± 0.331
2.601 ± 0.242
4.418 ± 0.171
3.91 ± 0.331
9.405 ± 0.406
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.517 ± 0.188
4.726 ± 0.468
6.026 ± 0.223
3.702 ± 0.228
7.25 ± 0.474
7.543 ± 0.261
6.011 ± 0.275
6.434 ± 0.233
1.408 ± 0.157
4.256 ± 0.31
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here