Sinirhodobacter hankyongi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Sinirhodobacter

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3573 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A421BVH7|A0A421BVH7_9RHOB Uncharacterized protein OS=Sinirhodobacter hankyongi OX=2294033 GN=DYS74_02615 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.19KK3 pKa = 10.41VLFATTALVFSAGFAAAEE21 pKa = 4.29VTLSGDD27 pKa = 2.64GRR29 pKa = 11.84MGVTYY34 pKa = 10.67NGSDD38 pKa = 3.05WNFSSRR44 pKa = 11.84ARR46 pKa = 11.84VTFTLSGQTDD56 pKa = 3.27SGLEE60 pKa = 3.86FGGSFRR66 pKa = 11.84ADD68 pKa = 3.14QAGDD72 pKa = 3.76SIANNGAAYY81 pKa = 8.16GTEE84 pKa = 3.68GSVYY88 pKa = 10.43VSGAFGKK95 pKa = 10.88LEE97 pKa = 4.01MGDD100 pKa = 3.46VVSAPEE106 pKa = 4.22ALFGDD111 pKa = 4.52LPEE114 pKa = 4.49VGYY117 pKa = 9.51TDD119 pKa = 5.38LVTSGAGLEE128 pKa = 4.0NDD130 pKa = 3.59IPYY133 pKa = 8.93LTLDD137 pKa = 3.96NNTQSALLYY146 pKa = 9.29TYY148 pKa = 9.09TAGAFSVAASLSDD161 pKa = 3.34GFVDD165 pKa = 4.09QTSVDD170 pKa = 3.32ANQAYY175 pKa = 9.27GVAAAYY181 pKa = 8.36TFGNYY186 pKa = 7.27TVGLGYY192 pKa = 10.76EE193 pKa = 4.22DD194 pKa = 5.47LEE196 pKa = 4.38LAAGGKK202 pKa = 6.62MTQAEE207 pKa = 4.48LAGVATFGDD216 pKa = 4.11TSVKK220 pKa = 10.28AYY222 pKa = 10.51YY223 pKa = 10.55AVGDD227 pKa = 4.31DD228 pKa = 5.23LNPVDD233 pKa = 3.94NSYY236 pKa = 11.34GLGVSSVFGATTVMGYY252 pKa = 7.08VQKK255 pKa = 11.37AEE257 pKa = 4.21FVGGADD263 pKa = 3.6VTWYY267 pKa = 10.93GLGAAYY273 pKa = 10.31DD274 pKa = 3.99LGGGASVVGGIADD287 pKa = 4.44DD288 pKa = 5.05DD289 pKa = 4.23IKK291 pKa = 11.43GSDD294 pKa = 3.52PVADD298 pKa = 4.38LGVKK302 pKa = 10.36FKK304 pKa = 11.05FF305 pKa = 3.81

Molecular weight: 31.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A421BXT2|A0A421BXT2_9RHOB Uncharacterized protein OS=Sinirhodobacter hankyongi OX=2294033 GN=DYS74_01890 PE=4 SV=1
MM1 pKa = 7.86PPRR4 pKa = 11.84TGPRR8 pKa = 11.84PKK10 pKa = 9.63ATRR13 pKa = 11.84KK14 pKa = 4.13TTPMRR19 pKa = 11.84RR20 pKa = 11.84SRR22 pKa = 11.84PRR24 pKa = 11.84SPRR27 pKa = 11.84PTRR30 pKa = 11.84MPRR33 pKa = 11.84SRR35 pKa = 11.84RR36 pKa = 11.84STRR39 pKa = 11.84CPRR42 pKa = 11.84RR43 pKa = 11.84KK44 pKa = 9.75RR45 pKa = 11.84PSLRR49 pKa = 11.84PRR51 pKa = 11.84KK52 pKa = 9.52APPKK56 pKa = 7.56ATRR59 pKa = 11.84WRR61 pKa = 11.84LPRR64 pKa = 11.84PLSPPRR70 pKa = 11.84RR71 pKa = 11.84ARR73 pKa = 11.84SRR75 pKa = 3.33

Molecular weight: 8.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3573

0

3573

1138740

22

1981

318.7

34.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.029 ± 0.075

0.873 ± 0.014

5.366 ± 0.033

5.954 ± 0.039

3.601 ± 0.023

9.043 ± 0.038

1.973 ± 0.02

4.767 ± 0.032

2.828 ± 0.034

10.503 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.02

2.143 ± 0.022

5.545 ± 0.037

2.776 ± 0.02

7.271 ± 0.039

4.595 ± 0.03

5.384 ± 0.025

7.279 ± 0.035

1.416 ± 0.017

2.037 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski