Streptococcus satellite phage Javan297

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZKJ9|A0A4D5ZKJ9_9VIRU Site-specific recombinase OS=Streptococcus satellite phage Javan297 OX=2558616 GN=JavanS297_0005 PE=3 SV=1
MM1 pKa = 7.61NNTQVYY7 pKa = 8.58QAQTTDD13 pKa = 2.95HH14 pKa = 6.68SIARR18 pKa = 11.84ALGEE22 pKa = 4.75EE23 pKa = 3.69IRR25 pKa = 11.84MITDD29 pKa = 3.35VEE31 pKa = 4.09EE32 pKa = 4.08TTYY35 pKa = 11.07NGHH38 pKa = 6.47GEE40 pKa = 3.97ITVEE44 pKa = 4.03YY45 pKa = 8.99TNDD48 pKa = 3.08VALTEE53 pKa = 4.07LFDD56 pKa = 5.14RR57 pKa = 11.84IKK59 pKa = 10.86SLYY62 pKa = 9.62EE63 pKa = 3.72KK64 pKa = 10.85GEE66 pKa = 4.17SYY68 pKa = 7.74EE69 pKa = 4.29TKK71 pKa = 10.16PFLHH75 pKa = 6.55FKK77 pKa = 10.57VLEE80 pKa = 4.74DD81 pKa = 4.36DD82 pKa = 4.8VEE84 pKa = 4.48PNPNGVFTAKK94 pKa = 10.16EE95 pKa = 4.05VVDD98 pKa = 4.16FLGII102 pKa = 3.62

Molecular weight: 11.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZNR9|A0A4D5ZNR9_9VIRU Virulence-associated protein E OS=Streptococcus satellite phage Javan297 OX=2558616 GN=JavanS297_0006 PE=4 SV=1
MM1 pKa = 7.92KK2 pKa = 9.25ITEE5 pKa = 4.18YY6 pKa = 10.54KK7 pKa = 10.41KK8 pKa = 10.18KK9 pKa = 10.4DD10 pKa = 3.2GSVVYY15 pKa = 10.34RR16 pKa = 11.84SSVYY20 pKa = 10.71LGIDD24 pKa = 3.15TVTGKK29 pKa = 10.24KK30 pKa = 10.26VKK32 pKa = 8.24TTISDD37 pKa = 3.44RR38 pKa = 11.84TKK40 pKa = 11.03NRR42 pKa = 11.84LKK44 pKa = 10.91SKK46 pKa = 10.43AIQAKK51 pKa = 9.85VEE53 pKa = 4.13FEE55 pKa = 4.56KK56 pKa = 11.26NGSTVTKK63 pKa = 8.73TVNVTTYY70 pKa = 10.95QEE72 pKa = 4.26LTNLWLEE79 pKa = 4.53NYY81 pKa = 9.36CHH83 pKa = 5.63TVKK86 pKa = 10.77HH87 pKa = 5.55STLIGAKK94 pKa = 10.27NNIKK98 pKa = 10.42NISYY102 pKa = 10.46QPLEE106 pKa = 4.2TTNN109 pKa = 3.29

Molecular weight: 12.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21

0

21

2987

49

392

142.2

16.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.055 ± 0.442

0.636 ± 0.088

6.227 ± 0.468

8.939 ± 0.609

4.553 ± 0.44

4.988 ± 0.375

1.875 ± 0.221

6.729 ± 0.696

10.211 ± 0.519

9.943 ± 0.678

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.544 ± 0.304

5.189 ± 0.384

2.343 ± 0.215

3.85 ± 0.355

5.658 ± 0.465

4.888 ± 0.419

6.093 ± 0.58

4.821 ± 0.611

0.703 ± 0.135

4.754 ± 0.393
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski