Cohnella sp. CAU 1483

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Cohnella

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4000 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U0FB07|A0A4U0FB07_9BACL Flagellar secretion chaperone FliS OS=Cohnella sp. CAU 1483 OX=2564100 GN=fliS PE=3 SV=1
MM1 pKa = 7.37NIEE4 pKa = 4.09RR5 pKa = 11.84AQQIAQSDD13 pKa = 3.98EE14 pKa = 4.22LVDD17 pKa = 3.96VIYY20 pKa = 11.05YY21 pKa = 8.72GASYY25 pKa = 10.92YY26 pKa = 9.85IDD28 pKa = 3.54QVDD31 pKa = 4.03EE32 pKa = 4.24TSGMARR38 pKa = 11.84IYY40 pKa = 10.84SRR42 pKa = 11.84EE43 pKa = 4.01NPDD46 pKa = 5.15DD47 pKa = 4.02PMQTVPVIALTEE59 pKa = 4.26PDD61 pKa = 3.5SFRR64 pKa = 11.84VV65 pKa = 3.46

Molecular weight: 7.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U0FAM6|A0A4U0FAM6_9BACL DEAD/DEAH box helicase OS=Cohnella sp. CAU 1483 OX=2564100 GN=E5161_13040 PE=4 SV=1
MM1 pKa = 7.61GPTFKK6 pKa = 10.87PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.27NGRR28 pKa = 11.84KK29 pKa = 8.13VLKK32 pKa = 10.34ARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.22GRR39 pKa = 11.84KK40 pKa = 8.88VLTAA44 pKa = 4.27

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4000

0

4000

1262812

11

3273

315.7

35.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.968 ± 0.047

0.743 ± 0.012

5.227 ± 0.027

6.602 ± 0.041

4.085 ± 0.03

7.778 ± 0.04

2.086 ± 0.02

6.112 ± 0.033

5.06 ± 0.033

10.02 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.791 ± 0.021

3.449 ± 0.029

4.379 ± 0.024

3.583 ± 0.021

5.865 ± 0.041

6.034 ± 0.033

5.298 ± 0.032

7.439 ± 0.033

1.325 ± 0.016

3.159 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski