Sphingomonas panacisoli

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3225 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8LE43|A0A5B8LE43_9SPHN Glutathione S-transferase family protein OS=Sphingomonas panacisoli OX=1813879 GN=FPZ24_01650 PE=4 SV=1
MM1 pKa = 7.29TVPTEE6 pKa = 3.99PVTPEE11 pKa = 3.29MLLRR15 pKa = 11.84RR16 pKa = 11.84FLYY19 pKa = 10.23GVNEE23 pKa = 4.28TPDD26 pKa = 3.79LLTDD30 pKa = 4.15RR31 pKa = 11.84LRR33 pKa = 11.84DD34 pKa = 3.61LAPTASISIGEE45 pKa = 4.38INSDD49 pKa = 3.82TYY51 pKa = 10.38LASYY55 pKa = 9.75GRR57 pKa = 11.84YY58 pKa = 9.12INAAYY63 pKa = 9.93FPFVQDD69 pKa = 3.9FFTGAKK75 pKa = 7.66TLSVGTHH82 pKa = 5.52TVAQLQSTYY91 pKa = 10.62GASEE95 pKa = 3.91FLYY98 pKa = 10.38TLSNANTDD106 pKa = 3.3VGSSDD111 pKa = 3.32FFLRR115 pKa = 11.84AFMFGHH121 pKa = 7.43ADD123 pKa = 3.43FHH125 pKa = 7.01LSPDD129 pKa = 3.49TQFVVDD135 pKa = 4.07TNGDD139 pKa = 3.5LHH141 pKa = 8.22IEE143 pKa = 4.02NMRR146 pKa = 11.84YY147 pKa = 9.49IPDD150 pKa = 3.25VDD152 pKa = 4.52NFDD155 pKa = 4.31FDD157 pKa = 4.11STGPTAIYY165 pKa = 10.16DD166 pKa = 3.71AVFAKK171 pKa = 10.67PEE173 pKa = 4.17IDD175 pKa = 3.4PSSIGRR181 pKa = 11.84QITWTYY187 pKa = 10.02SDD189 pKa = 3.58PTIVHH194 pKa = 6.2NGYY197 pKa = 9.58YY198 pKa = 9.25AANYY202 pKa = 8.0AANVLNNYY210 pKa = 9.82NITSNSIYY218 pKa = 10.42AGAIILSQSFGYY230 pKa = 9.66FNQWKK235 pKa = 10.38SEE237 pKa = 3.61GVYY240 pKa = 10.95EE241 pKa = 4.43NFRR244 pKa = 11.84DD245 pKa = 3.89NKK247 pKa = 9.5TIIYY251 pKa = 9.81DD252 pKa = 3.7GLGTNVVVADD262 pKa = 3.49ITSLHH267 pKa = 6.03GSILIGGDD275 pKa = 3.78GNDD278 pKa = 3.63QLIGAHH284 pKa = 6.27YY285 pKa = 10.97DD286 pKa = 3.37DD287 pKa = 4.71VFYY290 pKa = 11.2GGDD293 pKa = 3.59GNDD296 pKa = 3.77TIKK299 pKa = 10.78PGLGSSEE306 pKa = 3.82LWGGDD311 pKa = 3.43EE312 pKa = 4.11EE313 pKa = 5.28SKK315 pKa = 10.99PEE317 pKa = 4.3YY318 pKa = 10.3EE319 pKa = 4.6DD320 pKa = 3.98TDD322 pKa = 3.73TADD325 pKa = 3.71YY326 pKa = 9.26TGHH329 pKa = 6.07SAIDD333 pKa = 3.27ITFDD337 pKa = 3.25NSGEE341 pKa = 4.07KK342 pKa = 10.53LSLKK346 pKa = 9.44VAHH349 pKa = 7.21DD350 pKa = 3.87GANDD354 pKa = 3.75TLHH357 pKa = 7.12SIEE360 pKa = 4.62VIKK363 pKa = 9.32GTPATDD369 pKa = 3.11HH370 pKa = 6.13VKK372 pKa = 10.46IIGSIPTATMLTIDD386 pKa = 3.69AAGGQGPNPLDD397 pKa = 3.94TINLAKK403 pKa = 10.13ADD405 pKa = 3.92NGIHH409 pKa = 6.42VSIANDD415 pKa = 3.56GTGYY419 pKa = 9.9IHH421 pKa = 8.11DD422 pKa = 4.49KK423 pKa = 8.03TTEE426 pKa = 3.81GDD428 pKa = 3.14ILLAGFNTQIIGSAFDD444 pKa = 5.37DD445 pKa = 4.18VFDD448 pKa = 5.91DD449 pKa = 4.21DD450 pKa = 5.35AVGAKK455 pKa = 10.11HH456 pKa = 6.95IDD458 pKa = 3.19GGYY461 pKa = 10.75GNDD464 pKa = 3.85TISITGASDD473 pKa = 4.05DD474 pKa = 3.92SLLLGGAGNDD484 pKa = 4.14TITGGDD490 pKa = 3.77GNDD493 pKa = 3.46VLVGGAYY500 pKa = 9.99AYY502 pKa = 9.97GAYY505 pKa = 10.24SDD507 pKa = 4.06TLNGGGGNDD516 pKa = 3.48MLIGSGANDD525 pKa = 3.44IFDD528 pKa = 4.29GGDD531 pKa = 3.28GYY533 pKa = 11.47DD534 pKa = 3.89YY535 pKa = 10.8IRR537 pKa = 11.84PTAIPGTTFGLATITGGAGDD557 pKa = 4.16DD558 pKa = 3.98VINLRR563 pKa = 11.84EE564 pKa = 4.44GGDD567 pKa = 3.76HH568 pKa = 6.08YY569 pKa = 11.72NGMYY573 pKa = 10.06HH574 pKa = 6.63YY575 pKa = 10.76GAAPTLNFGANDD587 pKa = 3.43GHH589 pKa = 6.44DD590 pKa = 3.83TVLTASGGDD599 pKa = 3.35VFTAYY604 pKa = 10.76DD605 pKa = 3.53NGVLNDD611 pKa = 4.15HH612 pKa = 6.54GADD615 pKa = 4.18GIVVKK620 pKa = 10.91LDD622 pKa = 3.11GLYY625 pKa = 10.85ASDD628 pKa = 5.37IDD630 pKa = 4.68IIWDD634 pKa = 3.42VTPQPEE640 pKa = 4.39PFFGLGFNGNGDD652 pKa = 3.7LAIVVKK658 pKa = 9.88STGASIFIEE667 pKa = 4.42DD668 pKa = 3.88VNGSATFGNQSSSSNHH684 pKa = 5.9FGVTILDD691 pKa = 3.84GAGNEE696 pKa = 3.43IWIPSFPVQYY706 pKa = 11.03GDD708 pKa = 3.26LSSYY712 pKa = 10.71RR713 pKa = 11.84GGEE716 pKa = 3.53AAYY719 pKa = 10.17FDD721 pKa = 4.23AAALSSSEE729 pKa = 4.17STGTSGDD736 pKa = 3.5DD737 pKa = 3.38VMSGGRR743 pKa = 11.84GDD745 pKa = 5.3DD746 pKa = 4.01DD747 pKa = 5.91LSGGDD752 pKa = 3.66GNDD755 pKa = 3.22VFVSSPGNDD764 pKa = 3.32VIDD767 pKa = 4.78GGDD770 pKa = 3.63GSDD773 pKa = 3.26TLQLLGARR781 pKa = 11.84GDD783 pKa = 3.89YY784 pKa = 10.57TITASGAATLVEE796 pKa = 4.24GNAYY800 pKa = 10.12DD801 pKa = 4.12VGTLTLTSIEE811 pKa = 4.29NIRR814 pKa = 11.84FEE816 pKa = 5.03SDD818 pKa = 2.84DD819 pKa = 3.58ATYY822 pKa = 10.27TSQDD826 pKa = 2.86LFGYY830 pKa = 10.21YY831 pKa = 8.41GTAGDD836 pKa = 5.91DD837 pKa = 3.66VLLGNALGTNFYY849 pKa = 11.18ALAGDD854 pKa = 4.21DD855 pKa = 4.13QISPGIGNDD864 pKa = 3.38VVDD867 pKa = 4.72GGDD870 pKa = 3.75GEE872 pKa = 5.06DD873 pKa = 3.71EE874 pKa = 4.43VVLAASSANSSVSRR888 pKa = 11.84EE889 pKa = 3.79LDD891 pKa = 3.34GSISVYY897 pKa = 10.9DD898 pKa = 4.13PASGSYY904 pKa = 10.39DD905 pKa = 3.16ILWNVEE911 pKa = 3.8KK912 pKa = 10.59VTFLGDD918 pKa = 3.19STTIDD923 pKa = 3.66VGDD926 pKa = 4.73LPPLGTSGNDD936 pKa = 3.57TITGTARR943 pKa = 11.84GEE945 pKa = 4.08TLYY948 pKa = 11.21GQDD951 pKa = 4.92GDD953 pKa = 3.98DD954 pKa = 3.68QIYY957 pKa = 10.45GLGGNDD963 pKa = 4.02HH964 pKa = 7.11LNGGAGDD971 pKa = 4.34DD972 pKa = 4.39LLDD975 pKa = 4.76GGTGNNGLDD984 pKa = 3.78GGDD987 pKa = 4.22GNDD990 pKa = 3.8TLIGGAGQDD999 pKa = 2.99SMYY1002 pKa = 10.74GGEE1005 pKa = 4.61GSDD1008 pKa = 3.5TYY1010 pKa = 11.07IVGDD1014 pKa = 3.89AYY1016 pKa = 10.78GAFADD1021 pKa = 4.65TEE1023 pKa = 4.17VGDD1026 pKa = 3.83YY1027 pKa = 11.16GDD1029 pKa = 3.76YY1030 pKa = 11.5GNFVDD1035 pKa = 5.83HH1036 pKa = 7.36DD1037 pKa = 4.11TLALGDD1043 pKa = 3.75ATYY1046 pKa = 11.36SFAADD1051 pKa = 3.53GDD1053 pKa = 4.02RR1054 pKa = 11.84VIITNTATLHH1064 pKa = 5.36TVTLANQLSGYY1075 pKa = 8.57GVEE1078 pKa = 4.48SIEE1081 pKa = 4.21IGSNTVLDD1089 pKa = 4.3RR1090 pKa = 11.84DD1091 pKa = 5.18DD1092 pKa = 4.83ILDD1095 pKa = 5.33LIPVNVISGTSGDD1108 pKa = 3.99DD1109 pKa = 3.46QISGTNVNDD1118 pKa = 5.23DD1119 pKa = 3.27IAAGAGNDD1127 pKa = 3.93YY1128 pKa = 10.63IQEE1131 pKa = 4.2STGNDD1136 pKa = 2.99VYY1138 pKa = 10.94RR1139 pKa = 11.84WNVGDD1144 pKa = 3.85GDD1146 pKa = 4.59DD1147 pKa = 4.59YY1148 pKa = 11.62IAGGDD1153 pKa = 3.92SNDD1156 pKa = 3.88GYY1158 pKa = 11.52NSVVLGSGIATSDD1171 pKa = 3.43VTVSFTDD1178 pKa = 3.88FFEE1181 pKa = 4.5QSVLLSFAQGGSITLEE1197 pKa = 4.44NINGTDD1203 pKa = 3.43QNVDD1207 pKa = 3.18EE1208 pKa = 5.79VEE1210 pKa = 4.29FDD1212 pKa = 5.71DD1213 pKa = 3.83NTVWDD1218 pKa = 4.42RR1219 pKa = 11.84STLIGMAMDD1228 pKa = 3.69NSGGGGGGPLGLAANSKK1245 pKa = 8.86TGSFPAPHH1253 pKa = 6.25YY1254 pKa = 10.51RR1255 pKa = 11.84VDD1257 pKa = 3.4HH1258 pKa = 5.89NAVVSRR1264 pKa = 11.84TPVANSDD1271 pKa = 3.53VALDD1275 pKa = 4.02DD1276 pKa = 3.87FDD1278 pKa = 5.77YY1279 pKa = 10.85MGTARR1284 pKa = 11.84WAISKK1289 pKa = 10.6LSGYY1293 pKa = 10.98DD1294 pKa = 3.1EE1295 pKa = 4.12MVARR1299 pKa = 11.84KK1300 pKa = 9.57SRR1302 pKa = 11.84IAAGVSNRR1310 pKa = 11.84PFSGTDD1316 pKa = 2.81IAVSVAGNPSVNRR1329 pKa = 11.84SANLLAEE1336 pKa = 5.35AISTFDD1342 pKa = 3.22PTAAGGFSTSEE1353 pKa = 4.08DD1354 pKa = 3.09KK1355 pKa = 11.15GAPDD1359 pKa = 3.98NFWLFHH1365 pKa = 5.78QNGSLEE1371 pKa = 4.6SKK1373 pKa = 10.62SSLAMIAA1380 pKa = 3.71

Molecular weight: 143.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8LD45|A0A5B8LD45_9SPHN DUF4405 domain-containing protein OS=Sphingomonas panacisoli OX=1813879 GN=FPZ24_00220 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.0KK41 pKa = 10.58LSAA44 pKa = 4.03

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3225

0

3225

991115

29

1932

307.3

33.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.421 ± 0.059

0.729 ± 0.013

6.295 ± 0.034

4.876 ± 0.041

3.55 ± 0.027

8.932 ± 0.045

1.84 ± 0.023

5.107 ± 0.029

3.323 ± 0.035

9.511 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.437 ± 0.022

2.664 ± 0.035

5.403 ± 0.035

2.926 ± 0.023

7.061 ± 0.044

5.103 ± 0.034

5.692 ± 0.036

7.367 ± 0.034

1.487 ± 0.021

2.279 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski