Methanothrix thermoacetophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT) (Methanosaeta thermophila)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanosarcinales; Methanotrichaceae; Methanothrix; Methanothrix thermoacetophila

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1673 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A0B688|PRIS_METTP DNA primase small subunit PriS OS=Methanothrix thermoacetophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT) OX=349307 GN=priS PE=3 SV=1
MM1 pKa = 7.44TIKK4 pKa = 10.49FKK6 pKa = 11.13SSNDD10 pKa = 3.93DD11 pKa = 3.23ISCEE15 pKa = 4.01GGIKK19 pKa = 9.13TRR21 pKa = 11.84ILILMAVLITVSSAVADD38 pKa = 3.84PSYY41 pKa = 10.88GSKK44 pKa = 10.25VLPYY48 pKa = 10.67DD49 pKa = 3.83ADD51 pKa = 3.52EE52 pKa = 4.49SRR54 pKa = 11.84ALSAFSSAPVFAYY67 pKa = 10.38SDD69 pKa = 3.56ANIRR73 pKa = 11.84GIFDD77 pKa = 3.12ISDD80 pKa = 3.5PVYY83 pKa = 10.74IDD85 pKa = 4.0VDD87 pKa = 3.81PSDD90 pKa = 4.4DD91 pKa = 3.62AVNEE95 pKa = 3.83NDD97 pKa = 3.88VRR99 pKa = 11.84LTPFGSYY106 pKa = 9.71PAGSQVRR113 pKa = 11.84LGDD116 pKa = 3.09QDD118 pKa = 3.61YY119 pKa = 10.5GYY121 pKa = 10.97KK122 pKa = 9.25LTPFGVSGMPGAQLVYY138 pKa = 10.38FDD140 pKa = 4.24VDD142 pKa = 2.87GDD144 pKa = 3.77EE145 pKa = 5.21AYY147 pKa = 10.97SIVDD151 pKa = 3.31PVYY154 pKa = 10.85LDD156 pKa = 3.28VGPEE160 pKa = 3.81YY161 pKa = 11.25GRR163 pKa = 11.84IDD165 pKa = 3.69AGDD168 pKa = 3.6VRR170 pKa = 11.84ITGYY174 pKa = 7.6MTLQAGTRR182 pKa = 11.84VRR184 pKa = 11.84DD185 pKa = 3.59SDD187 pKa = 3.99PDD189 pKa = 3.54NDD191 pKa = 3.72KK192 pKa = 10.45PAPVLPAMLCFMNLNGNINSGGWAIYY218 pKa = 9.91DD219 pKa = 3.32QGDD222 pKa = 4.08RR223 pKa = 11.84IYY225 pKa = 11.49VDD227 pKa = 3.29TQYY230 pKa = 11.44PFYY233 pKa = 10.14TVTVNDD239 pKa = 2.78IRR241 pKa = 11.84LFVV244 pKa = 3.49

Molecular weight: 26.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0B9H1|A0B9H1_METTP Formylmethanofuran dehydrogenase subunit F OS=Methanothrix thermoacetophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT) OX=349307 GN=Mthe_1575 PE=4 SV=1
MM1 pKa = 7.19SKK3 pKa = 10.63KK4 pKa = 9.55MKK6 pKa = 9.46PKK8 pKa = 10.44KK9 pKa = 9.68IRR11 pKa = 11.84LAKK14 pKa = 10.35ALRR17 pKa = 11.84QNQRR21 pKa = 11.84VPLWVIMKK29 pKa = 7.07TKK31 pKa = 10.18RR32 pKa = 11.84RR33 pKa = 11.84VVSHH37 pKa = 6.35PKK39 pKa = 8.22RR40 pKa = 11.84RR41 pKa = 11.84HH42 pKa = 3.68WRR44 pKa = 11.84RR45 pKa = 11.84SSLEE49 pKa = 3.69

Molecular weight: 6.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1673

0

1673

508086

41

2713

303.7

33.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.308 ± 0.059

1.338 ± 0.024

5.817 ± 0.039

7.303 ± 0.067

3.286 ± 0.036

7.94 ± 0.055

1.842 ± 0.027

7.317 ± 0.047

4.022 ± 0.049

9.525 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.081 ± 0.032

2.921 ± 0.04

4.468 ± 0.033

2.119 ± 0.027

7.476 ± 0.064

6.702 ± 0.069

4.574 ± 0.064

7.837 ± 0.058

1.038 ± 0.027

3.087 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski