Amino acid dipepetide frequency for Methanothrix thermoacetophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT) (Methanosaeta thermophila)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.902AlaAla: 7.902 ± 0.166
1.002AlaCys: 1.002 ± 0.049
4.489AlaAsp: 4.489 ± 0.107
6.278AlaGlu: 6.278 ± 0.128
2.818AlaPhe: 2.818 ± 0.083
7.046AlaGly: 7.046 ± 0.12
1.384AlaHis: 1.384 ± 0.049
5.81AlaIle: 5.81 ± 0.116
3.062AlaLys: 3.062 ± 0.092
8.957AlaLeu: 8.957 ± 0.185
2.722AlaMet: 2.722 ± 0.077
2.086AlaAsn: 2.086 ± 0.075
2.905AlaPro: 2.905 ± 0.077
1.671AlaGln: 1.671 ± 0.062
6.154AlaArg: 6.154 ± 0.124
5.271AlaSer: 5.271 ± 0.12
3.653AlaThr: 3.653 ± 0.114
6.845AlaVal: 6.845 ± 0.13
0.795AlaTrp: 0.795 ± 0.048
2.224AlaTyr: 2.224 ± 0.065
0.0AlaXaa: 0.0 ± 0.0
Cys
1.047CysAla: 1.047 ± 0.048
0.234CysCys: 0.234 ± 0.022
0.803CysAsp: 0.803 ± 0.041
0.842CysGlu: 0.842 ± 0.041
0.419CysPhe: 0.419 ± 0.032
1.533CysGly: 1.533 ± 0.065
0.26CysHis: 0.26 ± 0.025
0.917CysIle: 0.917 ± 0.043
0.382CysLys: 0.382 ± 0.029
0.955CysLeu: 0.955 ± 0.037
0.423CysMet: 0.423 ± 0.029
0.394CysAsn: 0.394 ± 0.034
0.846CysPro: 0.846 ± 0.045
0.234CysGln: 0.234 ± 0.021
1.031CysArg: 1.031 ± 0.052
0.84CysSer: 0.84 ± 0.044
0.748CysThr: 0.748 ± 0.044
0.915CysVal: 0.915 ± 0.037
0.132CysTrp: 0.132 ± 0.016
0.419CysTyr: 0.419 ± 0.028
0.0CysXaa: 0.0 ± 0.0
Asp
5.365AspAla: 5.365 ± 0.113
0.62AspCys: 0.62 ± 0.033
2.85AspAsp: 2.85 ± 0.082
4.283AspGlu: 4.283 ± 0.106
1.756AspPhe: 1.756 ± 0.056
4.773AspGly: 4.773 ± 0.152
1.081AspHis: 1.081 ± 0.046
4.375AspIle: 4.375 ± 0.095
1.803AspLys: 1.803 ± 0.061
6.954AspLeu: 6.954 ± 0.132
1.681AspMet: 1.681 ± 0.06
1.279AspAsn: 1.279 ± 0.057
3.277AspPro: 3.277 ± 0.09
1.218AspGln: 1.218 ± 0.049
4.019AspArg: 4.019 ± 0.085
2.694AspSer: 2.694 ± 0.085
2.303AspThr: 2.303 ± 0.069
5.432AspVal: 5.432 ± 0.113
0.669AspTrp: 0.669 ± 0.04
1.738AspTyr: 1.738 ± 0.07
0.0AspXaa: 0.0 ± 0.0
Glu
5.633GluAla: 5.633 ± 0.139
0.799GluCys: 0.799 ± 0.042
3.883GluAsp: 3.883 ± 0.084
5.615GluGlu: 5.615 ± 0.131
2.212GluPhe: 2.212 ± 0.073
4.55GluGly: 4.55 ± 0.1
1.49GluHis: 1.49 ± 0.058
7.105GluIle: 7.105 ± 0.122
3.47GluLys: 3.47 ± 0.088
7.107GluLeu: 7.107 ± 0.142
2.858GluMet: 2.858 ± 0.094
2.399GluAsn: 2.399 ± 0.08
2.555GluPro: 2.555 ± 0.064
1.592GluGln: 1.592 ± 0.057
6.558GluArg: 6.558 ± 0.143
4.421GluSer: 4.421 ± 0.102
2.858GluThr: 2.858 ± 0.077
5.131GluVal: 5.131 ± 0.111
0.76GluTrp: 0.76 ± 0.041
2.033GluTyr: 2.033 ± 0.065
0.0GluXaa: 0.0 ± 0.0
Phe
2.565PheAla: 2.565 ± 0.066
0.429PheCys: 0.429 ± 0.03
2.261PheAsp: 2.261 ± 0.072
2.279PheGlu: 2.279 ± 0.076
1.203PhePhe: 1.203 ± 0.054
2.911PheGly: 2.911 ± 0.087
0.606PheHis: 0.606 ± 0.033
2.035PheIle: 2.035 ± 0.076
1.096PheLys: 1.096 ± 0.046
2.911PheLeu: 2.911 ± 0.083
0.976PheMet: 0.976 ± 0.04
0.921PheAsn: 0.921 ± 0.045
1.332PhePro: 1.332 ± 0.06
0.783PheGln: 0.783 ± 0.039
2.389PheArg: 2.389 ± 0.062
2.289PheSer: 2.289 ± 0.072
1.582PheThr: 1.582 ± 0.059
2.736PheVal: 2.736 ± 0.07
0.364PheTrp: 0.364 ± 0.031
1.193PheTyr: 1.193 ± 0.05
0.0PheXaa: 0.0 ± 0.0
Gly
6.578GlyAla: 6.578 ± 0.126
1.348GlyCys: 1.348 ± 0.055
4.834GlyAsp: 4.834 ± 0.134
5.369GlyGlu: 5.369 ± 0.104
2.903GlyPhe: 2.903 ± 0.069
5.761GlyGly: 5.761 ± 0.135
1.352GlyHis: 1.352 ± 0.05
6.542GlyIle: 6.542 ± 0.131
3.265GlyLys: 3.265 ± 0.09
6.753GlyLeu: 6.753 ± 0.114
2.828GlyMet: 2.828 ± 0.076
2.163GlyAsn: 2.163 ± 0.086
2.57GlyPro: 2.57 ± 0.073
1.378GlyGln: 1.378 ± 0.055
5.81GlyArg: 5.81 ± 0.116
5.548GlySer: 5.548 ± 0.129
3.952GlyThr: 3.952 ± 0.101
6.493GlyVal: 6.493 ± 0.12
0.972GlyTrp: 0.972 ± 0.05
2.98GlyTyr: 2.98 ± 0.082
0.0GlyXaa: 0.0 ± 0.0
His
1.401HisAla: 1.401 ± 0.049
0.285HisCys: 0.285 ± 0.024
0.996HisAsp: 0.996 ± 0.042
1.047HisGlu: 1.047 ± 0.046
0.596HisPhe: 0.596 ± 0.034
1.657HisGly: 1.657 ± 0.055
0.553HisHis: 0.553 ± 0.032
1.313HisIle: 1.313 ± 0.046
0.565HisLys: 0.565 ± 0.035
1.941HisLeu: 1.941 ± 0.064
0.547HisMet: 0.547 ± 0.031
0.518HisAsn: 0.518 ± 0.028
1.22HisPro: 1.22 ± 0.049
0.421HisGln: 0.421 ± 0.029
1.329HisArg: 1.329 ± 0.052
1.029HisSer: 1.029 ± 0.052
0.913HisThr: 0.913 ± 0.045
1.38HisVal: 1.38 ± 0.05
0.157HisTrp: 0.157 ± 0.019
0.549HisTyr: 0.549 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
6.558IleAla: 6.558 ± 0.133
1.147IleCys: 1.147 ± 0.055
4.491IleAsp: 4.491 ± 0.094
5.533IleGlu: 5.533 ± 0.106
2.675IlePhe: 2.675 ± 0.082
5.432IleGly: 5.432 ± 0.104
1.37IleHis: 1.37 ± 0.055
4.312IleIle: 4.312 ± 0.102
3.139IleLys: 3.139 ± 0.085
6.536IleLeu: 6.536 ± 0.133
1.86IleMet: 1.86 ± 0.059
2.185IleAsn: 2.185 ± 0.065
3.456IlePro: 3.456 ± 0.092
1.687IleGln: 1.687 ± 0.054
4.987IleArg: 4.987 ± 0.103
5.788IleSer: 5.788 ± 0.123
3.682IleThr: 3.682 ± 0.101
5.448IleVal: 5.448 ± 0.098
0.691IleTrp: 0.691 ± 0.034
2.193IleTyr: 2.193 ± 0.081
0.0IleXaa: 0.0 ± 0.0
Lys
3.287LysAla: 3.287 ± 0.092
0.384LysCys: 0.384 ± 0.034
2.411LysAsp: 2.411 ± 0.074
3.318LysGlu: 3.318 ± 0.086
0.955LysPhe: 0.955 ± 0.043
3.143LysGly: 3.143 ± 0.079
0.833LysHis: 0.833 ± 0.041
2.984LysIle: 2.984 ± 0.082
2.183LysLys: 2.183 ± 0.083
3.37LysLeu: 3.37 ± 0.087
1.171LysMet: 1.171 ± 0.048
1.484LysAsn: 1.484 ± 0.062
1.86LysPro: 1.86 ± 0.064
0.907LysGln: 0.907 ± 0.054
3.151LysArg: 3.151 ± 0.097
2.519LysSer: 2.519 ± 0.07
1.923LysThr: 1.923 ± 0.069
2.83LysVal: 2.83 ± 0.079
0.36LysTrp: 0.36 ± 0.028
1.144LysTyr: 1.144 ± 0.052
0.0LysXaa: 0.0 ± 0.0
Leu
7.912LeuAla: 7.912 ± 0.14
1.212LeuCys: 1.212 ± 0.053
5.702LeuAsp: 5.702 ± 0.12
7.385LeuGlu: 7.385 ± 0.121
2.909LeuPhe: 2.909 ± 0.084
7.552LeuGly: 7.552 ± 0.152
1.624LeuHis: 1.624 ± 0.053
6.32LeuIle: 6.32 ± 0.111
4.773LeuLys: 4.773 ± 0.113
9.012LeuLeu: 9.012 ± 0.193
2.755LeuMet: 2.755 ± 0.082
2.937LeuAsn: 2.937 ± 0.083
4.009LeuPro: 4.009 ± 0.087
2.008LeuGln: 2.008 ± 0.061
7.735LeuArg: 7.735 ± 0.142
7.324LeuSer: 7.324 ± 0.148
4.005LeuThr: 4.005 ± 0.098
6.702LeuVal: 6.702 ± 0.115
0.825LeuTrp: 0.825 ± 0.041
2.549LeuTyr: 2.549 ± 0.072
0.0LeuXaa: 0.0 ± 0.0
Met
2.529MetAla: 2.529 ± 0.093
0.358MetCys: 0.358 ± 0.027
1.925MetAsp: 1.925 ± 0.057
2.047MetGlu: 2.047 ± 0.068
0.716MetPhe: 0.716 ± 0.036
2.472MetGly: 2.472 ± 0.088
0.569MetHis: 0.569 ± 0.033
2.37MetIle: 2.37 ± 0.069
1.462MetLys: 1.462 ± 0.058
3.188MetLeu: 3.188 ± 0.076
0.943MetMet: 0.943 ± 0.047
1.134MetAsn: 1.134 ± 0.049
1.417MetPro: 1.417 ± 0.056
0.592MetGln: 0.592 ± 0.034
2.659MetArg: 2.659 ± 0.074
1.907MetSer: 1.907 ± 0.07
1.271MetThr: 1.271 ± 0.053
2.265MetVal: 2.265 ± 0.07
0.24MetTrp: 0.24 ± 0.021
0.74MetTyr: 0.74 ± 0.044
0.0MetXaa: 0.0 ± 0.0
Asn
2.773AsnAla: 2.773 ± 0.072
0.315AsnCys: 0.315 ± 0.036
1.305AsnAsp: 1.305 ± 0.056
1.598AsnGlu: 1.598 ± 0.067
0.856AsnPhe: 0.856 ± 0.044
2.222AsnGly: 2.222 ± 0.083
0.476AsnHis: 0.476 ± 0.03
2.342AsnIle: 2.342 ± 0.083
0.957AsnLys: 0.957 ± 0.048
3.094AsnLeu: 3.094 ± 0.089
0.811AsnMet: 0.811 ± 0.044
0.86AsnAsn: 0.86 ± 0.058
1.838AsnPro: 1.838 ± 0.07
0.703AsnGln: 0.703 ± 0.036
2.049AsnArg: 2.049 ± 0.069
1.59AsnSer: 1.59 ± 0.072
1.645AsnThr: 1.645 ± 0.072
2.543AsnVal: 2.543 ± 0.096
0.293AsnTrp: 0.293 ± 0.026
0.937AsnTyr: 0.937 ± 0.05
0.0AsnXaa: 0.0 ± 0.0
Pro
3.297ProAla: 3.297 ± 0.085
0.543ProCys: 0.543 ± 0.037
3.064ProAsp: 3.064 ± 0.09
4.625ProGlu: 4.625 ± 0.083
1.517ProPhe: 1.517 ± 0.057
4.06ProGly: 4.06 ± 0.096
0.88ProHis: 0.88 ± 0.041
2.319ProIle: 2.319 ± 0.067
1.626ProLys: 1.626 ± 0.05
3.991ProLeu: 3.991 ± 0.074
1.126ProMet: 1.126 ± 0.048
1.157ProAsn: 1.157 ± 0.056
2.086ProPro: 2.086 ± 0.067
1.008ProGln: 1.008 ± 0.059
2.48ProArg: 2.48 ± 0.068
2.805ProSer: 2.805 ± 0.08
1.874ProThr: 1.874 ± 0.071
4.332ProVal: 4.332 ± 0.106
0.504ProTrp: 0.504 ± 0.034
1.39ProTyr: 1.39 ± 0.054
0.0ProXaa: 0.0 ± 0.0
Gln
1.75GlnAla: 1.75 ± 0.064
0.22GlnCys: 0.22 ± 0.023
1.13GlnAsp: 1.13 ± 0.055
1.584GlnGlu: 1.584 ± 0.056
0.646GlnPhe: 0.646 ± 0.039
1.332GlnGly: 1.332 ± 0.047
0.396GlnHis: 0.396 ± 0.027
1.824GlnIle: 1.824 ± 0.063
0.947GlnLys: 0.947 ± 0.045
2.002GlnLeu: 2.002 ± 0.063
0.683GlnMet: 0.683 ± 0.037
0.752GlnAsn: 0.752 ± 0.042
0.886GlnPro: 0.886 ± 0.045
0.555GlnGln: 0.555 ± 0.039
1.769GlnArg: 1.769 ± 0.068
1.39GlnSer: 1.39 ± 0.052
0.97GlnThr: 0.97 ± 0.053
1.502GlnVal: 1.502 ± 0.056
0.222GlnTrp: 0.222 ± 0.029
0.632GlnTyr: 0.632 ± 0.045
0.0GlnXaa: 0.0 ± 0.0
Arg
6.265ArgAla: 6.265 ± 0.149
1.289ArgCys: 1.289 ± 0.063
4.55ArgAsp: 4.55 ± 0.108
5.629ArgGlu: 5.629 ± 0.118
2.769ArgPhe: 2.769 ± 0.078
5.464ArgGly: 5.464 ± 0.106
1.118ArgHis: 1.118 ± 0.052
6.214ArgIle: 6.214 ± 0.119
2.842ArgLys: 2.842 ± 0.071
6.631ArgLeu: 6.631 ± 0.115
2.565ArgMet: 2.565 ± 0.071
2.271ArgAsn: 2.271 ± 0.073
2.868ArgPro: 2.868 ± 0.074
1.236ArgGln: 1.236 ± 0.058
5.991ArgArg: 5.991 ± 0.134
5.454ArgSer: 5.454 ± 0.11
2.787ArgThr: 2.787 ± 0.076
5.267ArgVal: 5.267 ± 0.1
0.842ArgTrp: 0.842 ± 0.043
2.706ArgTyr: 2.706 ± 0.079
0.0ArgXaa: 0.0 ± 0.0
Ser
5.198SerAla: 5.198 ± 0.119
0.96SerCys: 0.96 ± 0.044
3.889SerAsp: 3.889 ± 0.103
4.541SerGlu: 4.541 ± 0.108
2.287SerPhe: 2.287 ± 0.066
6.273SerGly: 6.273 ± 0.159
1.206SerHis: 1.206 ± 0.047
4.865SerIle: 4.865 ± 0.127
2.427SerLys: 2.427 ± 0.068
6.034SerLeu: 6.034 ± 0.124
2.336SerMet: 2.336 ± 0.077
1.756SerAsn: 1.756 ± 0.074
3.181SerPro: 3.181 ± 0.104
1.496SerGln: 1.496 ± 0.052
4.991SerArg: 4.991 ± 0.102
4.724SerSer: 4.724 ± 0.139
3.047SerThr: 3.047 ± 0.084
4.918SerVal: 4.918 ± 0.112
0.791SerTrp: 0.791 ± 0.044
2.1SerTyr: 2.1 ± 0.094
0.0SerXaa: 0.0 ± 0.0
Thr
3.759ThrAla: 3.759 ± 0.11
0.624ThrCys: 0.624 ± 0.036
2.531ThrAsp: 2.531 ± 0.086
2.87ThrGlu: 2.87 ± 0.076
1.462ThrPhe: 1.462 ± 0.05
4.8ThrGly: 4.8 ± 0.139
0.854ThrHis: 0.854 ± 0.04
3.086ThrIle: 3.086 ± 0.089
1.61ThrLys: 1.61 ± 0.054
4.306ThrLeu: 4.306 ± 0.098
1.082ThrMet: 1.082 ± 0.049
1.391ThrAsn: 1.391 ± 0.058
2.657ThrPro: 2.657 ± 0.076
0.947ThrGln: 0.947 ± 0.047
2.622ThrArg: 2.622 ± 0.078
3.088ThrSer: 3.088 ± 0.101
2.431ThrThr: 2.431 ± 0.083
3.73ThrVal: 3.73 ± 0.103
0.496ThrTrp: 0.496 ± 0.047
1.395ThrTyr: 1.395 ± 0.05
0.0ThrXaa: 0.0 ± 0.0
Val
6.328ValAla: 6.328 ± 0.114
1.061ValCys: 1.061 ± 0.047
4.832ValAsp: 4.832 ± 0.088
5.751ValGlu: 5.751 ± 0.116
2.629ValPhe: 2.629 ± 0.074
5.243ValGly: 5.243 ± 0.11
1.443ValHis: 1.443 ± 0.055
5.501ValIle: 5.501 ± 0.098
3.108ValLys: 3.108 ± 0.089
7.514ValLeu: 7.514 ± 0.137
2.2ValMet: 2.2 ± 0.072
2.159ValAsn: 2.159 ± 0.08
3.572ValPro: 3.572 ± 0.097
1.693ValGln: 1.693 ± 0.063
5.686ValArg: 5.686 ± 0.112
5.473ValSer: 5.473 ± 0.107
3.743ValThr: 3.743 ± 0.123
7.168ValVal: 7.168 ± 0.139
0.789ValTrp: 0.789 ± 0.06
2.48ValTyr: 2.48 ± 0.076
0.0ValXaa: 0.0 ± 0.0
Trp
0.695TrpAla: 0.695 ± 0.038
0.13TrpCys: 0.13 ± 0.018
0.567TrpAsp: 0.567 ± 0.036
0.614TrpGlu: 0.614 ± 0.038
0.402TrpPhe: 0.402 ± 0.031
0.677TrpGly: 0.677 ± 0.037
0.22TrpHis: 0.22 ± 0.02
0.825TrpIle: 0.825 ± 0.051
0.455TrpLys: 0.455 ± 0.03
0.99TrpLeu: 0.99 ± 0.043
0.39TrpMet: 0.39 ± 0.026
0.413TrpAsn: 0.413 ± 0.03
0.35TrpPro: 0.35 ± 0.025
0.299TrpGln: 0.299 ± 0.026
0.821TrpArg: 0.821 ± 0.043
0.909TrpSer: 0.909 ± 0.089
0.468TrpThr: 0.468 ± 0.036
0.63TrpVal: 0.63 ± 0.035
0.165TrpTrp: 0.165 ± 0.019
0.364TrpTyr: 0.364 ± 0.038
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.236TyrAla: 2.236 ± 0.067
0.413TyrCys: 0.413 ± 0.029
1.655TyrAsp: 1.655 ± 0.051
1.821TyrGlu: 1.821 ± 0.065
1.132TyrPhe: 1.132 ± 0.052
2.498TyrGly: 2.498 ± 0.078
0.705TyrHis: 0.705 ± 0.038
2.112TyrIle: 2.112 ± 0.07
1.045TyrLys: 1.045 ± 0.068
3.003TyrLeu: 3.003 ± 0.083
0.846TyrMet: 0.846 ± 0.04
0.949TyrAsn: 0.949 ± 0.05
1.797TyrPro: 1.797 ± 0.064
0.764TyrGln: 0.764 ± 0.051
2.525TyrArg: 2.525 ± 0.08
1.956TyrSer: 1.956 ± 0.071
1.886TyrThr: 1.886 ± 0.079
2.108TyrVal: 2.108 ± 0.061
0.315TyrTrp: 0.315 ± 0.026
1.102TyrTyr: 1.102 ± 0.051
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1673 proteins (508086 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski