Aquareovirus G (isolate American grass carp/USA/PB01-155/-) (AQRV-G)

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Aquareovirus; Aquareovirus G; American grass carp reovirus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|B2BNE5|VNS5_AQRVG Non-structural protein 5 OS=Aquareovirus G (isolate American grass carp/USA/PB01-155/-) OX=648234 GN=S7 PE=3 SV=1
MM1 pKa = 7.55GNVQTSTNVYY11 pKa = 10.5NIDD14 pKa = 3.52GNGNTFAPSSQMASTASPAIDD35 pKa = 4.29LKK37 pKa = 11.1PGVLNPTGKK46 pKa = 10.19LWQTMGTGAPSADD59 pKa = 3.4SLVLVVDD66 pKa = 3.85NKK68 pKa = 11.33GEE70 pKa = 3.97YY71 pKa = 9.6TYY73 pKa = 11.03LSEE76 pKa = 5.31NMRR79 pKa = 11.84EE80 pKa = 4.21TLNKK84 pKa = 10.32AVTDD88 pKa = 3.74VNMWQPLFQATKK100 pKa = 8.9SGCGPVVLANFTTISTGYY118 pKa = 10.29VGATADD124 pKa = 3.69DD125 pKa = 4.2AFSNGLVSNGPFLATMHH142 pKa = 6.35IMEE145 pKa = 4.61LQKK148 pKa = 10.04TIAARR153 pKa = 11.84MRR155 pKa = 11.84DD156 pKa = 3.39VAIWQKK162 pKa = 10.95HH163 pKa = 5.88LDD165 pKa = 3.52TAMTLMTPDD174 pKa = 3.43VSAGDD179 pKa = 3.97VTCKK183 pKa = 9.32WRR185 pKa = 11.84SLLEE189 pKa = 3.92FAQDD193 pKa = 4.42ILPLDD198 pKa = 4.03NLCRR202 pKa = 11.84SYY204 pKa = 11.29PNEE207 pKa = 4.2FYY209 pKa = 10.56TVAAQRR215 pKa = 11.84YY216 pKa = 5.68PAIRR220 pKa = 11.84PGQPDD225 pKa = 3.68TQVALPQPHH234 pKa = 7.45PLGEE238 pKa = 4.25VAGSFNAPTSEE249 pKa = 3.94VGSLVGAGAALSDD262 pKa = 5.11AISTLASKK270 pKa = 10.92DD271 pKa = 3.48LDD273 pKa = 3.73LVEE276 pKa = 5.92ADD278 pKa = 3.57TPLPVSVFTPSLAPRR293 pKa = 11.84TYY295 pKa = 10.02RR296 pKa = 11.84PAFIDD301 pKa = 3.88PQDD304 pKa = 4.07AAWIAQWNGDD314 pKa = 3.42ANIRR318 pKa = 11.84IITTYY323 pKa = 10.6QSTDD327 pKa = 3.17YY328 pKa = 10.24TVQLGPGPTRR338 pKa = 11.84VIDD341 pKa = 3.64MNAMIDD347 pKa = 3.49AKK349 pKa = 10.34LTLDD353 pKa = 3.39VSGTILPFQEE363 pKa = 4.58NNDD366 pKa = 3.65LSSAIPAFVLIQTKK380 pKa = 9.77VPLHH384 pKa = 6.41SVTQASDD391 pKa = 3.46VEE393 pKa = 4.8GITVVSAAEE402 pKa = 3.86SSAINLSVNVRR413 pKa = 11.84GDD415 pKa = 3.17PRR417 pKa = 11.84FDD419 pKa = 3.45MLHH422 pKa = 5.57LHH424 pKa = 7.01AMFEE428 pKa = 4.4RR429 pKa = 11.84EE430 pKa = 4.26TIAGIPYY437 pKa = 9.5IYY439 pKa = 10.63GIGTFLIPSITSSSSFCNPTLMDD462 pKa = 4.05GEE464 pKa = 4.58LTVTPLLLRR473 pKa = 11.84EE474 pKa = 4.17TTYY477 pKa = 11.06KK478 pKa = 10.62GAVVDD483 pKa = 4.02TVTPSEE489 pKa = 4.17VMANQTSEE497 pKa = 4.21EE498 pKa = 4.25VASALANDD506 pKa = 3.73AVLLVSGQLEE516 pKa = 3.94RR517 pKa = 11.84LATVVGDD524 pKa = 4.16VIPIASGEE532 pKa = 3.98DD533 pKa = 3.41DD534 pKa = 3.99AATSAIVGRR543 pKa = 11.84LAIEE547 pKa = 3.63ATMRR551 pKa = 11.84ARR553 pKa = 11.84HH554 pKa = 5.81GGDD557 pKa = 2.91TRR559 pKa = 11.84ALPNFGQLWKK569 pKa = 9.72RR570 pKa = 11.84AKK572 pKa = 10.02RR573 pKa = 11.84AASMFASNPALALQVGVPVLADD595 pKa = 3.52SGILSALTSGVSTAIRR611 pKa = 11.84TGSLGKK617 pKa = 10.43GVSDD621 pKa = 5.09ASSKK625 pKa = 11.0LNARR629 pKa = 11.84QSLTLARR636 pKa = 11.84KK637 pKa = 7.16TFFKK641 pKa = 10.52KK642 pKa = 10.69VEE644 pKa = 4.23EE645 pKa = 4.27LWPSQQ650 pKa = 3.26

Molecular weight: 68.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|B2BNE8|VNS2_AQRVG Non-structural protein 2 OS=Aquareovirus G (isolate American grass carp/USA/PB01-155/-) OX=648234 GN=S9 PE=3 SV=1
MM1 pKa = 7.71ALRR4 pKa = 11.84RR5 pKa = 11.84FLRR8 pKa = 11.84TSPITSTAQPAPLYY22 pKa = 10.43DD23 pKa = 3.64NQDD26 pKa = 2.96IQDD29 pKa = 3.33IVGAYY34 pKa = 8.68ARR36 pKa = 11.84PWQSRR41 pKa = 11.84FGDD44 pKa = 3.34ITITTTSAPVWSGRR58 pKa = 11.84YY59 pKa = 8.71PSVAARR65 pKa = 11.84NIIVNTILGAHH76 pKa = 6.8LNAFSGGVIAQYY88 pKa = 10.75RR89 pKa = 11.84GLTWRR94 pKa = 11.84DD95 pKa = 3.22NIMSSLAPPSQNPPPPAWVPAEE117 pKa = 3.85NVQLDD122 pKa = 3.8SDD124 pKa = 4.5NYY126 pKa = 8.95PQYY129 pKa = 11.75ALNLSKK135 pKa = 9.71MWSVNLDD142 pKa = 3.31VHH144 pKa = 8.13IMTMWALSDD153 pKa = 3.77YY154 pKa = 10.64GPLYY158 pKa = 10.01EE159 pKa = 4.96ISVPTAPMPAMTTAALMAYY178 pKa = 8.74IGCSITQLAMTAYY191 pKa = 9.79QYY193 pKa = 11.46AGQLPQTAAATMTTTLRR210 pKa = 11.84WLAAIWFGSLCGVVHH225 pKa = 7.32RR226 pKa = 11.84NHH228 pKa = 6.37TVNGFYY234 pKa = 10.55FDD236 pKa = 3.67FGKK239 pKa = 10.0PGFNPDD245 pKa = 3.12HH246 pKa = 7.19AVLKK250 pKa = 10.27WNDD253 pKa = 3.38GNRR256 pKa = 11.84AAPPAAARR264 pKa = 11.84FRR266 pKa = 11.84VYY268 pKa = 10.33RR269 pKa = 11.84VRR271 pKa = 11.84SPHH274 pKa = 4.7WQQMTSEE281 pKa = 4.32VAGAILAQSVTAVAGLTAMFNNRR304 pKa = 11.84GLPVWAQNIPHH315 pKa = 6.1FTGAAAGTRR324 pKa = 11.84VSRR327 pKa = 11.84TYY329 pKa = 11.41NPVTMAAARR338 pKa = 11.84HH339 pKa = 5.28QNWQAAGLITAVKK352 pKa = 9.91QAEE355 pKa = 4.36LDD357 pKa = 3.61QQYY360 pKa = 10.17TDD362 pKa = 3.45YY363 pKa = 11.24AQAIEE368 pKa = 4.17VHH370 pKa = 6.01LTAQLAANPVANGRR384 pKa = 11.84MPIQPFLPADD394 pKa = 4.26FAAAGGTNQVVADD407 pKa = 4.26ARR409 pKa = 11.84LMFPP413 pKa = 4.57

Molecular weight: 44.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

7577

141

1298

631.4

69.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.703 ± 0.556

1.28 ± 0.175

5.147 ± 0.148

3.405 ± 0.396

3.062 ± 0.269

4.896 ± 0.335

2.732 ± 0.334

5.16 ± 0.222

1.98 ± 0.399

9.74 ± 0.382

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.679 ± 0.175

4.078 ± 0.434

7.958 ± 0.306

4.078 ± 0.154

5.728 ± 0.32

7.444 ± 0.553

8.42 ± 0.35

7.008 ± 0.314

1.412 ± 0.224

3.088 ± 0.39
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski