Burkholderiales bacterium JOSHI_001

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; unclassified Burkholderiales

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4891 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H5WRA7|H5WRA7_9BURK Ribosome-associated heat shock protein implicated in recycling of 50S subunit OS=Burkholderiales bacterium JOSHI_001 OX=864051 GN=BurJ1DRAFT_2905 PE=3 SV=1
MM1 pKa = 7.94DD2 pKa = 3.57VFKK5 pKa = 10.78TPGAFSWCEE14 pKa = 3.7LNVADD19 pKa = 4.62PAKK22 pKa = 10.47AAEE25 pKa = 4.42FYY27 pKa = 11.05GGLFGWTFDD36 pKa = 4.04TMDD39 pKa = 4.18MGQGPYY45 pKa = 10.06RR46 pKa = 11.84VIKK49 pKa = 10.3MGDD52 pKa = 3.32EE53 pKa = 3.96ALGGIMAMAPDD64 pKa = 5.23GPPVPMWGCYY74 pKa = 7.43ITVADD79 pKa = 4.44CDD81 pKa = 3.72KK82 pKa = 10.51TAAAATAAGGTVCAPPFDD100 pKa = 3.91IPGVGRR106 pKa = 11.84MAVLQDD112 pKa = 3.37PQGAVFNVIAYY123 pKa = 9.22SMPGG127 pKa = 2.98

Molecular weight: 13.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H5WMQ3|H5WMQ3_9BURK Ni Fe-hydrogenase I large subunit OS=Burkholderiales bacterium JOSHI_001 OX=864051 GN=BurJ1DRAFT_1609 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.07IRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSQVV45 pKa = 2.54

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4891

0

4891

1693128

30

6969

346.2

37.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.139 ± 0.056

0.904 ± 0.014

5.353 ± 0.029

4.723 ± 0.038

3.24 ± 0.02

8.574 ± 0.042

2.273 ± 0.024

3.424 ± 0.027

2.916 ± 0.038

11.282 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.256 ± 0.021

2.524 ± 0.035

5.69 ± 0.038

4.25 ± 0.025

7.149 ± 0.053

5.11 ± 0.034

5.082 ± 0.054

7.682 ± 0.029

1.55 ± 0.016

1.878 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski