Clostridium phage phiCPV4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Guelinviridae; Brucesealvirus; Clostridium virus phiCPV4

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I3PV42|I3PV42_9CAUD Uncharacterized protein OS=Clostridium phage phiCPV4 OX=1162305 GN=phiCPV4_0012 PE=4 SV=1
MM1 pKa = 7.8IDD3 pKa = 3.12LGKK6 pKa = 10.95VKK8 pKa = 10.99GEE10 pKa = 4.02DD11 pKa = 3.01AVVNSNHH18 pKa = 6.17NGLLGMVTLNDD29 pKa = 3.73KK30 pKa = 9.67TCLQWGYY37 pKa = 7.81FTITKK42 pKa = 9.84GNTWEE47 pKa = 3.96RR48 pKa = 11.84VNINVPYY55 pKa = 10.6KK56 pKa = 10.42DD57 pKa = 4.05DD58 pKa = 3.75NYY60 pKa = 10.59NIQVTEE66 pKa = 4.27YY67 pKa = 10.36YY68 pKa = 10.42VDD70 pKa = 3.91GYY72 pKa = 10.88KK73 pKa = 9.99PVYY76 pKa = 9.47IGNIDD81 pKa = 3.31KK82 pKa = 11.17AGFSVSTDD90 pKa = 3.02GDD92 pKa = 2.99IDD94 pKa = 4.24APGGKK99 pKa = 9.44QNIYY103 pKa = 8.45WFCIGVVEE111 pKa = 4.5

Molecular weight: 12.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3PV56|I3PV56_9CAUD Uncharacterized protein OS=Clostridium phage phiCPV4 OX=1162305 GN=phiCPV4_0026 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.22EE3 pKa = 3.79VIIVNKK9 pKa = 10.01VNGEE13 pKa = 3.88INRR16 pKa = 11.84VGLYY20 pKa = 10.29VVDD23 pKa = 3.75TNRR26 pKa = 11.84RR27 pKa = 11.84MKK29 pKa = 8.4WVKK32 pKa = 8.4WLRR35 pKa = 11.84KK36 pKa = 9.08KK37 pKa = 10.63DD38 pKa = 3.5YY39 pKa = 10.81RR40 pKa = 11.84EE41 pKa = 3.5YY42 pKa = 11.39ALINKK47 pKa = 8.73VEE49 pKa = 4.12ILEE52 pKa = 4.07QEE54 pKa = 3.98II55 pKa = 3.84

Molecular weight: 6.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

28

0

28

5430

46

727

193.9

22.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.702 ± 0.29

1.657 ± 0.221

6.335 ± 0.297

7.477 ± 0.416

4.346 ± 0.4

7.385 ± 0.62

1.639 ± 0.224

7.459 ± 0.389

8.674 ± 0.704

7.201 ± 0.496

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.278 ± 0.375

7.551 ± 0.545

2.689 ± 0.267

3.131 ± 0.328

3.923 ± 0.341

4.88 ± 0.402

5.654 ± 0.419

6.28 ± 0.406

1.215 ± 0.133

5.525 ± 0.436
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski