Amino acid dipepetide frequency for Clostridium phage phiCPV4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.0AlaAla: 0.0 ± 0.0
0.553AlaCys: 0.553 ± 0.332
1.658AlaAsp: 1.658 ± 0.519
2.395AlaGlu: 2.395 ± 0.669
2.395AlaPhe: 2.395 ± 0.564
1.658AlaGly: 1.658 ± 0.574
0.921AlaHis: 0.921 ± 0.464
3.131AlaIle: 3.131 ± 0.993
3.131AlaLys: 3.131 ± 0.774
3.131AlaLeu: 3.131 ± 0.806
1.105AlaMet: 1.105 ± 0.729
2.21AlaAsn: 2.21 ± 0.566
1.842AlaPro: 1.842 ± 0.677
1.474AlaGln: 1.474 ± 0.804
1.658AlaArg: 1.658 ± 0.528
2.21AlaSer: 2.21 ± 0.861
2.579AlaThr: 2.579 ± 0.729
2.21AlaVal: 2.21 ± 0.787
0.737AlaTrp: 0.737 ± 0.314
2.026AlaTyr: 2.026 ± 0.587
0.0AlaXaa: 0.0 ± 0.0
Cys
0.553CysAla: 0.553 ± 0.262
0.368CysCys: 0.368 ± 0.216
1.105CysAsp: 1.105 ± 0.492
0.921CysGlu: 0.921 ± 0.39
0.921CysPhe: 0.921 ± 0.421
1.658CysGly: 1.658 ± 0.52
0.184CysHis: 0.184 ± 0.192
0.737CysIle: 0.737 ± 0.324
2.21CysLys: 2.21 ± 0.907
2.763CysLeu: 2.763 ± 0.537
0.184CysMet: 0.184 ± 0.2
0.921CysAsn: 0.921 ± 0.399
0.737CysPro: 0.737 ± 0.424
0.553CysGln: 0.553 ± 0.363
0.368CysArg: 0.368 ± 0.263
0.184CysSer: 0.184 ± 0.156
0.553CysThr: 0.553 ± 0.34
0.921CysVal: 0.921 ± 0.375
0.0CysTrp: 0.0 ± 0.0
0.737CysTyr: 0.737 ± 0.336
0.0CysXaa: 0.0 ± 0.0
Asp
2.026AspAla: 2.026 ± 0.727
1.289AspCys: 1.289 ± 0.588
2.947AspAsp: 2.947 ± 0.85
5.526AspGlu: 5.526 ± 1.067
2.763AspPhe: 2.763 ± 0.676
4.605AspGly: 4.605 ± 1.3
1.105AspHis: 1.105 ± 0.35
4.973AspIle: 4.973 ± 0.664
6.815AspLys: 6.815 ± 1.153
4.421AspLeu: 4.421 ± 1.202
2.026AspMet: 2.026 ± 0.617
4.052AspAsn: 4.052 ± 0.971
2.21AspPro: 2.21 ± 0.575
0.553AspGln: 0.553 ± 0.306
3.131AspArg: 3.131 ± 0.902
2.026AspSer: 2.026 ± 0.604
3.5AspThr: 3.5 ± 0.822
5.526AspVal: 5.526 ± 1.169
0.921AspTrp: 0.921 ± 0.429
2.947AspTyr: 2.947 ± 0.753
0.0AspXaa: 0.0 ± 0.0
Glu
0.184GluAla: 0.184 ± 0.181
0.921GluCys: 0.921 ± 0.423
2.947GluAsp: 2.947 ± 0.781
6.078GluGlu: 6.078 ± 1.277
3.131GluPhe: 3.131 ± 0.803
6.447GluGly: 6.447 ± 1.49
1.658GluHis: 1.658 ± 0.568
4.973GluIle: 4.973 ± 1.06
7.368GluLys: 7.368 ± 1.256
5.71GluLeu: 5.71 ± 1.234
3.868GluMet: 3.868 ± 1.1
6.447GluAsn: 6.447 ± 1.001
2.21GluPro: 2.21 ± 0.505
1.842GluGln: 1.842 ± 0.449
3.316GluArg: 3.316 ± 0.947
2.579GluSer: 2.579 ± 0.808
3.316GluThr: 3.316 ± 1.025
6.999GluVal: 6.999 ± 1.025
0.921GluTrp: 0.921 ± 0.47
3.684GluTyr: 3.684 ± 0.97
0.0GluXaa: 0.0 ± 0.0
Phe
2.026PheAla: 2.026 ± 0.505
0.737PheCys: 0.737 ± 0.302
4.052PheAsp: 4.052 ± 0.853
4.052PheGlu: 4.052 ± 0.769
2.763PhePhe: 2.763 ± 0.675
2.395PheGly: 2.395 ± 0.738
0.368PheHis: 0.368 ± 0.311
3.5PheIle: 3.5 ± 1.138
3.316PheLys: 3.316 ± 0.675
3.131PheLeu: 3.131 ± 0.548
1.658PheMet: 1.658 ± 0.71
3.5PheAsn: 3.5 ± 0.859
1.658PhePro: 1.658 ± 0.578
1.105PheGln: 1.105 ± 0.349
0.737PheArg: 0.737 ± 0.464
2.21PheSer: 2.21 ± 0.55
1.289PheThr: 1.289 ± 0.612
2.763PheVal: 2.763 ± 0.64
0.553PheTrp: 0.553 ± 0.338
1.658PheTyr: 1.658 ± 0.721
0.0PheXaa: 0.0 ± 0.0
Gly
4.789GlyAla: 4.789 ± 1.232
1.105GlyCys: 1.105 ± 0.477
5.71GlyAsp: 5.71 ± 1.379
3.5GlyGlu: 3.5 ± 0.882
4.237GlyPhe: 4.237 ± 0.873
4.052GlyGly: 4.052 ± 1.152
1.105GlyHis: 1.105 ± 0.525
5.526GlyIle: 5.526 ± 0.965
7.552GlyLys: 7.552 ± 1.218
6.078GlyLeu: 6.078 ± 1.143
2.579GlyMet: 2.579 ± 0.823
5.71GlyAsn: 5.71 ± 1.426
0.0GlyPro: 0.0 ± 0.0
2.395GlyGln: 2.395 ± 0.641
2.026GlyArg: 2.026 ± 0.966
2.763GlySer: 2.763 ± 0.65
3.868GlyThr: 3.868 ± 1.352
5.71GlyVal: 5.71 ± 1.419
1.105GlyTrp: 1.105 ± 0.483
4.052GlyTyr: 4.052 ± 1.019
0.0GlyXaa: 0.0 ± 0.0
His
0.737HisAla: 0.737 ± 0.293
0.368HisCys: 0.368 ± 0.265
0.921HisAsp: 0.921 ± 0.46
1.105HisGlu: 1.105 ± 0.475
1.105HisPhe: 1.105 ± 0.372
1.105HisGly: 1.105 ± 0.364
0.368HisHis: 0.368 ± 0.27
1.289HisIle: 1.289 ± 0.503
1.842HisLys: 1.842 ± 0.596
0.921HisLeu: 0.921 ± 0.474
1.105HisMet: 1.105 ± 0.426
1.105HisAsn: 1.105 ± 0.48
0.368HisPro: 0.368 ± 0.246
0.0HisGln: 0.0 ± 0.0
0.184HisArg: 0.184 ± 0.194
1.289HisSer: 1.289 ± 0.49
0.368HisThr: 0.368 ± 0.311
1.289HisVal: 1.289 ± 0.613
0.184HisTrp: 0.184 ± 0.191
0.737HisTyr: 0.737 ± 0.377
0.0HisXaa: 0.0 ± 0.0
Ile
2.947IleAla: 2.947 ± 0.717
0.737IleCys: 0.737 ± 0.36
6.263IleAsp: 6.263 ± 1.369
7.184IleGlu: 7.184 ± 1.166
2.21IlePhe: 2.21 ± 0.491
6.263IleGly: 6.263 ± 0.917
0.737IleHis: 0.737 ± 0.298
5.71IleIle: 5.71 ± 1.03
6.263IleLys: 6.263 ± 0.906
4.605IleLeu: 4.605 ± 0.796
2.947IleMet: 2.947 ± 0.653
7.552IleAsn: 7.552 ± 1.891
2.763IlePro: 2.763 ± 0.846
2.21IleGln: 2.21 ± 0.824
1.658IleArg: 1.658 ± 0.527
2.579IleSer: 2.579 ± 0.674
5.71IleThr: 5.71 ± 0.906
3.131IleVal: 3.131 ± 0.862
0.184IleTrp: 0.184 ± 0.2
2.947IleTyr: 2.947 ± 0.774
0.0IleXaa: 0.0 ± 0.0
Lys
4.237LysAla: 4.237 ± 0.626
1.289LysCys: 1.289 ± 0.444
6.815LysAsp: 6.815 ± 0.926
7.368LysGlu: 7.368 ± 1.109
3.131LysPhe: 3.131 ± 0.742
6.999LysGly: 6.999 ± 1.052
1.474LysHis: 1.474 ± 0.601
4.973LysIle: 4.973 ± 1.236
6.447LysLys: 6.447 ± 1.718
6.078LysLeu: 6.078 ± 0.955
3.5LysMet: 3.5 ± 0.651
6.263LysAsn: 6.263 ± 1.282
2.395LysPro: 2.395 ± 0.605
4.052LysGln: 4.052 ± 1.036
2.763LysArg: 2.763 ± 0.758
3.131LysSer: 3.131 ± 0.744
4.605LysThr: 4.605 ± 1.38
4.052LysVal: 4.052 ± 0.865
1.105LysTrp: 1.105 ± 0.556
6.078LysTyr: 6.078 ± 1.05
0.0LysXaa: 0.0 ± 0.0
Leu
2.579LeuAla: 2.579 ± 0.845
1.658LeuCys: 1.658 ± 0.354
5.894LeuAsp: 5.894 ± 1.037
5.157LeuGlu: 5.157 ± 0.877
2.395LeuPhe: 2.395 ± 0.579
4.789LeuGly: 4.789 ± 0.904
1.105LeuHis: 1.105 ± 0.556
5.71LeuIle: 5.71 ± 1.074
6.263LeuLys: 6.263 ± 0.89
6.078LeuLeu: 6.078 ± 1.001
2.21LeuMet: 2.21 ± 0.884
5.894LeuAsn: 5.894 ± 0.876
1.289LeuPro: 1.289 ± 0.449
3.684LeuGln: 3.684 ± 1.322
3.131LeuArg: 3.131 ± 0.745
2.395LeuSer: 2.395 ± 0.588
3.5LeuThr: 3.5 ± 0.694
4.605LeuVal: 4.605 ± 1.023
0.921LeuTrp: 0.921 ± 0.427
2.763LeuTyr: 2.763 ± 0.509
0.0LeuXaa: 0.0 ± 0.0
Met
2.026MetAla: 2.026 ± 0.889
0.368MetCys: 0.368 ± 0.229
2.579MetAsp: 2.579 ± 0.708
2.763MetGlu: 2.763 ± 0.791
1.289MetPhe: 1.289 ± 0.347
1.474MetGly: 1.474 ± 0.524
0.0MetHis: 0.0 ± 0.0
2.763MetIle: 2.763 ± 0.647
2.947MetLys: 2.947 ± 0.865
2.21MetLeu: 2.21 ± 0.82
0.184MetMet: 0.184 ± 0.181
2.947MetAsn: 2.947 ± 0.643
1.289MetPro: 1.289 ± 0.484
1.474MetGln: 1.474 ± 0.736
0.737MetArg: 0.737 ± 0.352
2.026MetSer: 2.026 ± 0.708
1.842MetThr: 1.842 ± 0.534
1.289MetVal: 1.289 ± 0.503
0.184MetTrp: 0.184 ± 0.192
2.395MetTyr: 2.395 ± 0.806
0.0MetXaa: 0.0 ± 0.0
Asn
2.947AsnAla: 2.947 ± 0.501
1.105AsnCys: 1.105 ± 0.46
3.131AsnAsp: 3.131 ± 0.862
6.078AsnGlu: 6.078 ± 1.187
1.658AsnPhe: 1.658 ± 0.64
6.631AsnGly: 6.631 ± 1.093
1.474AsnHis: 1.474 ± 0.503
5.894AsnIle: 5.894 ± 1.13
6.447AsnLys: 6.447 ± 0.816
5.526AsnLeu: 5.526 ± 1.477
3.316AsnMet: 3.316 ± 0.68
5.157AsnAsn: 5.157 ± 0.995
1.842AsnPro: 1.842 ± 0.707
1.842AsnGln: 1.842 ± 0.577
3.684AsnArg: 3.684 ± 0.738
4.237AsnSer: 4.237 ± 0.853
5.526AsnThr: 5.526 ± 0.974
3.316AsnVal: 3.316 ± 0.667
1.105AsnTrp: 1.105 ± 0.415
4.605AsnTyr: 4.605 ± 1.352
0.0AsnXaa: 0.0 ± 0.0
Pro
0.737ProAla: 0.737 ± 0.571
0.368ProCys: 0.368 ± 0.259
0.368ProAsp: 0.368 ± 0.254
0.737ProGlu: 0.737 ± 0.325
0.921ProPhe: 0.921 ± 0.423
2.947ProGly: 2.947 ± 1.284
0.368ProHis: 0.368 ± 0.271
2.21ProIle: 2.21 ± 0.5
2.395ProLys: 2.395 ± 0.787
1.842ProLeu: 1.842 ± 0.41
0.553ProMet: 0.553 ± 0.283
1.658ProAsn: 1.658 ± 0.616
0.737ProPro: 0.737 ± 0.295
1.105ProGln: 1.105 ± 0.464
0.368ProArg: 0.368 ± 0.317
1.842ProSer: 1.842 ± 0.499
1.842ProThr: 1.842 ± 0.614
2.947ProVal: 2.947 ± 0.705
0.0ProTrp: 0.0 ± 0.0
2.947ProTyr: 2.947 ± 0.728
0.0ProXaa: 0.0 ± 0.0
Gln
2.579GlnAla: 2.579 ± 1.013
0.737GlnCys: 0.737 ± 0.299
0.921GlnAsp: 0.921 ± 0.44
1.289GlnGlu: 1.289 ± 0.399
1.474GlnPhe: 1.474 ± 0.472
3.316GlnGly: 3.316 ± 0.746
0.368GlnHis: 0.368 ± 0.273
2.026GlnIle: 2.026 ± 0.884
2.579GlnLys: 2.579 ± 0.981
1.842GlnLeu: 1.842 ± 0.72
1.105GlnMet: 1.105 ± 0.428
2.579GlnAsn: 2.579 ± 0.584
0.184GlnPro: 0.184 ± 0.17
1.105GlnGln: 1.105 ± 0.552
1.474GlnArg: 1.474 ± 0.501
2.026GlnSer: 2.026 ± 0.546
2.21GlnThr: 2.21 ± 0.597
1.289GlnVal: 1.289 ± 0.55
0.184GlnTrp: 0.184 ± 0.198
2.026GlnTyr: 2.026 ± 0.631
0.0GlnXaa: 0.0 ± 0.0
Arg
0.737ArgAla: 0.737 ± 0.284
1.105ArgCys: 1.105 ± 0.43
1.842ArgAsp: 1.842 ± 0.661
4.237ArgGlu: 4.237 ± 0.589
1.658ArgPhe: 1.658 ± 0.633
3.131ArgGly: 3.131 ± 0.834
0.553ArgHis: 0.553 ± 0.417
3.5ArgIle: 3.5 ± 0.888
3.316ArgLys: 3.316 ± 0.956
2.947ArgLeu: 2.947 ± 0.727
0.737ArgMet: 0.737 ± 0.356
2.21ArgAsn: 2.21 ± 0.671
0.921ArgPro: 0.921 ± 0.405
0.737ArgGln: 0.737 ± 0.403
2.947ArgArg: 2.947 ± 1.021
1.105ArgSer: 1.105 ± 0.45
2.026ArgThr: 2.026 ± 0.648
2.395ArgVal: 2.395 ± 0.605
1.105ArgTrp: 1.105 ± 0.39
2.026ArgTyr: 2.026 ± 0.458
0.0ArgXaa: 0.0 ± 0.0
Ser
3.131SerAla: 3.131 ± 0.987
0.737SerCys: 0.737 ± 0.299
1.842SerAsp: 1.842 ± 0.629
2.026SerGlu: 2.026 ± 0.782
2.21SerPhe: 2.21 ± 0.623
2.947SerGly: 2.947 ± 1.015
0.921SerHis: 0.921 ± 0.425
3.5SerIle: 3.5 ± 0.752
4.052SerLys: 4.052 ± 0.792
2.947SerLeu: 2.947 ± 0.811
1.474SerMet: 1.474 ± 0.779
3.316SerAsn: 3.316 ± 0.702
1.842SerPro: 1.842 ± 0.567
2.395SerGln: 2.395 ± 0.541
1.658SerArg: 1.658 ± 0.497
4.973SerSer: 4.973 ± 1.458
2.763SerThr: 2.763 ± 0.793
3.316SerVal: 3.316 ± 0.768
0.553SerTrp: 0.553 ± 0.286
2.21SerTyr: 2.21 ± 0.593
0.0SerXaa: 0.0 ± 0.0
Thr
1.289ThrAla: 1.289 ± 0.395
1.105ThrCys: 1.105 ± 0.423
4.789ThrAsp: 4.789 ± 0.82
3.5ThrGlu: 3.5 ± 0.72
1.842ThrPhe: 1.842 ± 0.539
5.71ThrGly: 5.71 ± 1.013
1.105ThrHis: 1.105 ± 0.481
5.157ThrIle: 5.157 ± 0.852
4.605ThrLys: 4.605 ± 0.881
2.395ThrLeu: 2.395 ± 0.661
1.474ThrMet: 1.474 ± 0.91
3.316ThrAsn: 3.316 ± 0.721
1.658ThrPro: 1.658 ± 0.709
2.026ThrGln: 2.026 ± 0.641
1.658ThrArg: 1.658 ± 0.483
3.5ThrSer: 3.5 ± 0.862
3.316ThrThr: 3.316 ± 1.021
4.237ThrVal: 4.237 ± 0.976
0.921ThrTrp: 0.921 ± 0.464
2.947ThrTyr: 2.947 ± 1.006
0.0ThrXaa: 0.0 ± 0.0
Val
1.658ValAla: 1.658 ± 0.5
0.921ValCys: 0.921 ± 0.364
5.342ValAsp: 5.342 ± 0.976
6.078ValGlu: 6.078 ± 1.131
2.947ValPhe: 2.947 ± 0.795
3.131ValGly: 3.131 ± 1.036
0.921ValHis: 0.921 ± 0.383
4.237ValIle: 4.237 ± 1.007
4.052ValLys: 4.052 ± 0.75
3.684ValLeu: 3.684 ± 0.59
1.105ValMet: 1.105 ± 0.416
6.631ValAsn: 6.631 ± 1.065
1.105ValPro: 1.105 ± 0.5
1.289ValGln: 1.289 ± 0.395
3.684ValArg: 3.684 ± 0.937
4.421ValSer: 4.421 ± 1.001
3.868ValThr: 3.868 ± 0.743
2.579ValVal: 2.579 ± 0.64
0.921ValTrp: 0.921 ± 0.467
4.237ValTyr: 4.237 ± 0.823
0.0ValXaa: 0.0 ± 0.0
Trp
0.184TrpAla: 0.184 ± 0.181
0.184TrpCys: 0.184 ± 0.192
0.737TrpAsp: 0.737 ± 0.391
1.289TrpGlu: 1.289 ± 0.378
0.553TrpPhe: 0.553 ± 0.35
0.737TrpGly: 0.737 ± 0.361
0.368TrpHis: 0.368 ± 0.274
1.289TrpIle: 1.289 ± 0.408
0.921TrpLys: 0.921 ± 0.468
1.658TrpLeu: 1.658 ± 0.706
0.184TrpMet: 0.184 ± 0.197
1.105TrpAsn: 1.105 ± 0.401
0.0TrpPro: 0.0 ± 0.0
0.368TrpGln: 0.368 ± 0.241
0.737TrpArg: 0.737 ± 0.308
0.368TrpSer: 0.368 ± 0.285
0.553TrpThr: 0.553 ± 0.291
0.368TrpVal: 0.368 ± 0.27
0.0TrpTrp: 0.0 ± 0.0
0.553TrpTyr: 0.553 ± 0.296
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.658TyrAla: 1.658 ± 0.531
0.921TyrCys: 0.921 ± 0.451
3.5TyrAsp: 3.5 ± 0.922
3.5TyrGlu: 3.5 ± 0.625
3.868TyrPhe: 3.868 ± 0.571
3.868TyrGly: 3.868 ± 1.002
1.289TyrHis: 1.289 ± 0.392
3.5TyrIle: 3.5 ± 0.86
4.237TyrLys: 4.237 ± 1.018
4.052TyrLeu: 4.052 ± 0.673
1.289TyrMet: 1.289 ± 0.499
2.947TyrAsn: 2.947 ± 0.532
1.842TyrPro: 1.842 ± 0.804
1.105TyrGln: 1.105 ± 0.419
3.316TyrArg: 3.316 ± 0.859
2.947TyrSer: 2.947 ± 0.894
3.316TyrThr: 3.316 ± 0.765
3.868TyrVal: 3.868 ± 0.86
0.553TyrTrp: 0.553 ± 0.325
3.684TyrTyr: 3.684 ± 0.944
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 28 proteins (5430 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski