Tomato spotted wilt virus (strain Brazilian Br-01) (TSWV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Tospoviridae; Orthotospovirus; Tomato spotted wilt orthotospovirus

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>sp|P36292|MVP_TSWV1 Movement protein OS=Tomato spotted wilt virus (strain Brazilian Br-01) OX=36413 GN=NSM PE=1 SV=1
MM1 pKa = 8.07RR2 pKa = 11.84ILKK5 pKa = 10.16LLEE8 pKa = 4.1LVVKK12 pKa = 10.77VSLFTIALSSVLLAFLIFRR31 pKa = 11.84ATDD34 pKa = 3.16AKK36 pKa = 11.21VEE38 pKa = 4.6IIRR41 pKa = 11.84GDD43 pKa = 3.36HH44 pKa = 6.45PEE46 pKa = 4.58IYY48 pKa = 10.19DD49 pKa = 3.8DD50 pKa = 3.73SAEE53 pKa = 4.25NEE55 pKa = 4.32VPTAASIQRR64 pKa = 11.84EE65 pKa = 4.53AILEE69 pKa = 4.19TLTNLMLEE77 pKa = 4.12SRR79 pKa = 11.84TPGTRR84 pKa = 11.84QIRR87 pKa = 11.84EE88 pKa = 4.19EE89 pKa = 4.31KK90 pKa = 9.32STIPISAEE98 pKa = 3.36PTTQKK103 pKa = 9.84TISVLDD109 pKa = 3.98LPNNCLNASSLKK121 pKa = 10.81CEE123 pKa = 3.92IKK125 pKa = 10.82GISTYY130 pKa = 10.21NVYY133 pKa = 10.69YY134 pKa = 10.07QVEE137 pKa = 4.49NNGVIYY143 pKa = 10.8SCVSDD148 pKa = 3.56SAEE151 pKa = 3.94GLEE154 pKa = 4.84KK155 pKa = 10.64CDD157 pKa = 4.77NSLNLPKK164 pKa = 10.19RR165 pKa = 11.84FSKK168 pKa = 11.04VPVIPITKK176 pKa = 9.34LDD178 pKa = 3.67KK179 pKa = 10.45KK180 pKa = 10.44RR181 pKa = 11.84HH182 pKa = 5.08FSVGGKK188 pKa = 9.35FFISEE193 pKa = 4.47SLTQDD198 pKa = 3.27NYY200 pKa = 10.44PITYY204 pKa = 10.25NSYY207 pKa = 8.05PTNGTVSLQTVKK219 pKa = 10.92LSGDD223 pKa = 3.73CKK225 pKa = 8.95ITKK228 pKa = 10.41SNFANPYY235 pKa = 7.71TVSITSPEE243 pKa = 4.14KK244 pKa = 10.56IMGYY248 pKa = 9.72LIKK251 pKa = 10.73KK252 pKa = 8.4PGEE255 pKa = 4.15NVEE258 pKa = 4.25HH259 pKa = 6.5KK260 pKa = 10.57VISFSGSASITFTEE274 pKa = 4.05EE275 pKa = 3.82MLDD278 pKa = 3.8GEE280 pKa = 4.97HH281 pKa = 7.13NLLCGDD287 pKa = 4.49KK288 pKa = 10.39SAKK291 pKa = 9.51IPKK294 pKa = 7.78TNKK297 pKa = 9.47RR298 pKa = 11.84VRR300 pKa = 11.84DD301 pKa = 4.09CIIKK305 pKa = 9.92YY306 pKa = 10.28SKK308 pKa = 10.42SIYY311 pKa = 9.82KK312 pKa = 8.12QTACINFSWIRR323 pKa = 11.84LILIALLIYY332 pKa = 9.71FPIRR336 pKa = 11.84WLVNKK341 pKa = 6.89TTKK344 pKa = 10.24PLFLWYY350 pKa = 10.16DD351 pKa = 3.47LMGLITYY358 pKa = 7.79PVLLLINCLWKK369 pKa = 10.73YY370 pKa = 10.8FPLKK374 pKa = 10.56CSNCGNLCIVTHH386 pKa = 6.83EE387 pKa = 4.23CTKK390 pKa = 10.65VCICNKK396 pKa = 9.76SKK398 pKa = 10.84ASKK401 pKa = 9.28EE402 pKa = 4.09HH403 pKa = 6.89SSEE406 pKa = 4.12CPILSKK412 pKa = 10.84EE413 pKa = 4.31ADD415 pKa = 3.19HH416 pKa = 7.78DD417 pKa = 4.85YY418 pKa = 11.53NKK420 pKa = 10.73HH421 pKa = 5.34KK422 pKa = 7.35WTSMEE427 pKa = 3.86WFHH430 pKa = 7.65LIVNTKK436 pKa = 10.66LSLSLLKK443 pKa = 10.42FVTEE447 pKa = 3.8ILIGLVILSQMPMSMAQTTQCLSGCFYY474 pKa = 11.5VPGCPFLVTSKK485 pKa = 10.89FEE487 pKa = 4.07KK488 pKa = 10.5CSEE491 pKa = 4.27KK492 pKa = 10.76DD493 pKa = 3.04QCYY496 pKa = 10.93CNVKK500 pKa = 9.39EE501 pKa = 4.39DD502 pKa = 4.58KK503 pKa = 10.43IIEE506 pKa = 4.64SIFGTNIVIEE516 pKa = 4.57GPNDD520 pKa = 3.85CIEE523 pKa = 4.21NQNCIARR530 pKa = 11.84PSIDD534 pKa = 3.83NLIKK538 pKa = 10.66CRR540 pKa = 11.84LGCEE544 pKa = 3.97YY545 pKa = 11.34LDD547 pKa = 3.94LFRR550 pKa = 11.84NKK552 pKa = 9.74PLYY555 pKa = 10.78NGFSDD560 pKa = 3.99YY561 pKa = 10.76TGSSLGLTSVGLYY574 pKa = 6.99EE575 pKa = 5.1AKK577 pKa = 10.36RR578 pKa = 11.84LRR580 pKa = 11.84NGIIDD585 pKa = 3.57SYY587 pKa = 11.19NRR589 pKa = 11.84QGKK592 pKa = 9.0ISGMVAGDD600 pKa = 3.75SLNKK604 pKa = 10.31NEE606 pKa = 4.13TSIPEE611 pKa = 4.38NILPRR616 pKa = 11.84QSLIFDD622 pKa = 3.65SVVDD626 pKa = 3.86GKK628 pKa = 10.61YY629 pKa = 10.2RR630 pKa = 11.84YY631 pKa = 9.37MIEE634 pKa = 3.82QSLLGGGGTIFMLNDD649 pKa = 3.37KK650 pKa = 10.19TSEE653 pKa = 4.02TAKK656 pKa = 10.75KK657 pKa = 9.2FVIYY661 pKa = 9.85IKK663 pKa = 10.68SVGIHH668 pKa = 5.25YY669 pKa = 7.95EE670 pKa = 3.82VSEE673 pKa = 4.82KK674 pKa = 10.2YY675 pKa = 6.08TTAPIQSTHH684 pKa = 5.09TDD686 pKa = 4.13FYY688 pKa = 10.5STCTGNCDD696 pKa = 3.29TCRR699 pKa = 11.84KK700 pKa = 8.9NQALTGFQDD709 pKa = 3.98FCVTPTSYY717 pKa = 9.64WGCEE721 pKa = 3.99EE722 pKa = 4.13AWCFAINEE730 pKa = 4.33GATCGFCRR738 pKa = 11.84NIYY741 pKa = 11.16DD742 pKa = 3.19MDD744 pKa = 3.57KK745 pKa = 10.63SYY747 pKa = 11.13RR748 pKa = 11.84IYY750 pKa = 11.05SVLKK754 pKa = 7.88STIVADD760 pKa = 3.74VCISGILGGQCSRR773 pKa = 11.84ITEE776 pKa = 4.1EE777 pKa = 3.99VPYY780 pKa = 10.8EE781 pKa = 4.0NTLFQADD788 pKa = 3.65IQADD792 pKa = 3.72LHH794 pKa = 6.74NDD796 pKa = 4.41GITIGEE802 pKa = 4.79LIAHH806 pKa = 6.91GPDD809 pKa = 2.87SHH811 pKa = 8.27IYY813 pKa = 9.69SGNIANLNDD822 pKa = 3.64PVKK825 pKa = 10.45MFGHH829 pKa = 6.71PQLTHH834 pKa = 6.86DD835 pKa = 4.72GVPIFTKK842 pKa = 9.49KK843 pKa = 8.73TLEE846 pKa = 4.3GDD848 pKa = 3.78DD849 pKa = 4.42MSWDD853 pKa = 3.46CAAIGKK859 pKa = 10.08KK860 pKa = 10.03SVTIKK865 pKa = 9.08TCGYY869 pKa = 7.19DD870 pKa = 3.12TYY872 pKa = 11.1RR873 pKa = 11.84FRR875 pKa = 11.84SGLEE879 pKa = 3.83QISDD883 pKa = 3.53IPVSFKK889 pKa = 11.02DD890 pKa = 4.1FSSFFLAKK898 pKa = 10.23SFSLGKK904 pKa = 10.49LKK906 pKa = 10.28MVVDD910 pKa = 5.16LPSDD914 pKa = 4.11LFKK917 pKa = 11.04VAPKK921 pKa = 10.12KK922 pKa = 10.48PSITSTSLNCNGCLLCGQGLSCLLEE947 pKa = 4.18FFSDD951 pKa = 3.84LTFSTAISIDD961 pKa = 3.38ACSLSTYY968 pKa = 10.34QLAVKK973 pKa = 10.24KK974 pKa = 10.77GSNKK978 pKa = 9.93YY979 pKa = 9.98NITMFCSANPDD990 pKa = 3.51KK991 pKa = 11.19KK992 pKa = 11.22KK993 pKa = 8.28MTLYY997 pKa = 10.5PEE999 pKa = 4.64GNPDD1003 pKa = 2.79ISVEE1007 pKa = 4.08VLVNNVIVEE1016 pKa = 4.15EE1017 pKa = 4.41PEE1019 pKa = 4.43NIIDD1023 pKa = 4.1QNDD1026 pKa = 3.55EE1027 pKa = 4.09YY1028 pKa = 11.62AHH1030 pKa = 7.07EE1031 pKa = 4.17EE1032 pKa = 3.71QQYY1035 pKa = 11.29NSDD1038 pKa = 3.28SSAWGFWDD1046 pKa = 4.25YY1047 pKa = 11.26IKK1049 pKa = 11.24SPFNFIASYY1058 pKa = 10.19FGSFFDD1064 pKa = 4.18TIRR1067 pKa = 11.84VVLLIAFIFLVTYY1080 pKa = 8.97FCSILTSICKK1090 pKa = 10.1GYY1092 pKa = 10.85VKK1094 pKa = 10.71NEE1096 pKa = 4.1SYY1098 pKa = 11.01KK1099 pKa = 10.61SRR1101 pKa = 11.84SKK1103 pKa = 10.74IEE1105 pKa = 5.15DD1106 pKa = 3.66DD1107 pKa = 4.89DD1108 pKa = 4.19EE1109 pKa = 5.95PEE1111 pKa = 4.02IKK1113 pKa = 10.62APMLMKK1119 pKa = 10.14DD1120 pKa = 2.78TMTRR1124 pKa = 11.84RR1125 pKa = 11.84RR1126 pKa = 11.84PPMDD1130 pKa = 3.92FSHH1133 pKa = 6.98LVV1135 pKa = 3.07

Molecular weight:
127.32 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>sp|P26002|NSS_TSWV1 Non-structural protein NS-S OS=Tomato spotted wilt virus (strain Brazilian Br-01) OX=36413 GN=NSS PE=3 SV=1
MM1 pKa = 7.46SKK3 pKa = 10.67VKK5 pKa = 9.03LTKK8 pKa = 10.26EE9 pKa = 4.24SIVALLTQGKK19 pKa = 9.34DD20 pKa = 3.27LEE22 pKa = 4.41FEE24 pKa = 4.0EE25 pKa = 4.91DD26 pKa = 3.4QNLVAFNFKK35 pKa = 9.02TFCLEE40 pKa = 4.82NIDD43 pKa = 4.24QIKK46 pKa = 9.75KK47 pKa = 9.55MSVISCLTFLKK58 pKa = 10.47NRR60 pKa = 11.84QSIMKK65 pKa = 9.42VIKK68 pKa = 10.61QSDD71 pKa = 3.68FTFGKK76 pKa = 8.34ITIKK80 pKa = 9.65KK81 pKa = 7.41TSDD84 pKa = 3.43RR85 pKa = 11.84IGGTDD90 pKa = 3.05MTFRR94 pKa = 11.84RR95 pKa = 11.84LDD97 pKa = 3.1SLIRR101 pKa = 11.84VRR103 pKa = 11.84LVEE106 pKa = 3.92EE107 pKa = 4.2TGNSEE112 pKa = 4.09NLNTIKK118 pKa = 10.74SKK120 pKa = 10.47IASHH124 pKa = 6.64PLIQAYY130 pKa = 9.08GLPLDD135 pKa = 4.27DD136 pKa = 4.82AKK138 pKa = 10.88SVRR141 pKa = 11.84LAIMLGGSLPLIASVDD157 pKa = 3.86SFEE160 pKa = 4.77MISVVLAIYY169 pKa = 10.45QDD171 pKa = 3.56AKK173 pKa = 11.32YY174 pKa = 9.92KK175 pKa = 10.82DD176 pKa = 3.89LGIDD180 pKa = 3.32PKK182 pKa = 11.13KK183 pKa = 10.89YY184 pKa = 8.9DD185 pKa = 3.46TKK187 pKa = 10.41EE188 pKa = 3.85ALGKK192 pKa = 10.3VCTVLKK198 pKa = 10.78SKK200 pKa = 10.62AFEE203 pKa = 4.09MNEE206 pKa = 3.92DD207 pKa = 3.39QVKK210 pKa = 10.35KK211 pKa = 9.85GKK213 pKa = 9.96EE214 pKa = 3.56YY215 pKa = 10.95AAILSSSNPNAKK227 pKa = 9.99GSVAMEE233 pKa = 4.65HH234 pKa = 6.45YY235 pKa = 10.83SEE237 pKa = 4.38TLNKK241 pKa = 9.16FYY243 pKa = 11.18EE244 pKa = 4.16MFGVKK249 pKa = 9.56KK250 pKa = 9.59QAKK253 pKa = 8.86LAEE256 pKa = 4.27LAA258 pKa = 4.02

Molecular weight:
28.84 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

5033

258

2875

1006.6

114.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.093 ± 0.48

2.205 ± 0.597

5.682 ± 0.356

6.994 ± 0.737

4.391 ± 0.263

4.331 ± 0.525

1.669 ± 0.22

8.146 ± 0.257

8.782 ± 0.59

9.974 ± 0.533

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.782 ± 0.343

6.279 ± 0.44

3.04 ± 0.587

2.662 ± 0.359

3.715 ± 0.546

9.338 ± 0.578

5.762 ± 0.392

5.424 ± 0.379

0.854 ± 0.197

3.874 ± 0.356

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski