Tomato spotted wilt virus (strain Brazilian Br-01) (TSWV)
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P36292|MVP_TSWV1 Movement protein OS=Tomato spotted wilt virus (strain Brazilian Br-01) OX=36413 GN=NSM PE=1 SV=1
MM1 pKa = 8.07 RR2 pKa = 11.84 ILKK5 pKa = 10.16 LLEE8 pKa = 4.1 LVVKK12 pKa = 10.77 VSLFTIALSSVLLAFLIFRR31 pKa = 11.84 ATDD34 pKa = 3.16 AKK36 pKa = 11.21 VEE38 pKa = 4.6 IIRR41 pKa = 11.84 GDD43 pKa = 3.36 HH44 pKa = 6.45 PEE46 pKa = 4.58 IYY48 pKa = 10.19 DD49 pKa = 3.8 DD50 pKa = 3.73 SAEE53 pKa = 4.25 NEE55 pKa = 4.32 VPTAASIQRR64 pKa = 11.84 EE65 pKa = 4.53 AILEE69 pKa = 4.19 TLTNLMLEE77 pKa = 4.12 SRR79 pKa = 11.84 TPGTRR84 pKa = 11.84 QIRR87 pKa = 11.84 EE88 pKa = 4.19 EE89 pKa = 4.31 KK90 pKa = 9.32 STIPISAEE98 pKa = 3.36 PTTQKK103 pKa = 9.84 TISVLDD109 pKa = 3.98 LPNNCLNASSLKK121 pKa = 10.81 CEE123 pKa = 3.92 IKK125 pKa = 10.82 GISTYY130 pKa = 10.21 NVYY133 pKa = 10.69 YY134 pKa = 10.07 QVEE137 pKa = 4.49 NNGVIYY143 pKa = 10.8 SCVSDD148 pKa = 3.56 SAEE151 pKa = 3.94 GLEE154 pKa = 4.84 KK155 pKa = 10.64 CDD157 pKa = 4.77 NSLNLPKK164 pKa = 10.19 RR165 pKa = 11.84 FSKK168 pKa = 11.04 VPVIPITKK176 pKa = 9.34 LDD178 pKa = 3.67 KK179 pKa = 10.45 KK180 pKa = 10.44 RR181 pKa = 11.84 HH182 pKa = 5.08 FSVGGKK188 pKa = 9.35 FFISEE193 pKa = 4.47 SLTQDD198 pKa = 3.27 NYY200 pKa = 10.44 PITYY204 pKa = 10.25 NSYY207 pKa = 8.05 PTNGTVSLQTVKK219 pKa = 10.92 LSGDD223 pKa = 3.73 CKK225 pKa = 8.95 ITKK228 pKa = 10.41 SNFANPYY235 pKa = 7.71 TVSITSPEE243 pKa = 4.14 KK244 pKa = 10.56 IMGYY248 pKa = 9.72 LIKK251 pKa = 10.73 KK252 pKa = 8.4 PGEE255 pKa = 4.15 NVEE258 pKa = 4.25 HH259 pKa = 6.5 KK260 pKa = 10.57 VISFSGSASITFTEE274 pKa = 4.05 EE275 pKa = 3.82 MLDD278 pKa = 3.8 GEE280 pKa = 4.97 HH281 pKa = 7.13 NLLCGDD287 pKa = 4.49 KK288 pKa = 10.39 SAKK291 pKa = 9.51 IPKK294 pKa = 7.78 TNKK297 pKa = 9.47 RR298 pKa = 11.84 VRR300 pKa = 11.84 DD301 pKa = 4.09 CIIKK305 pKa = 9.92 YY306 pKa = 10.28 SKK308 pKa = 10.42 SIYY311 pKa = 9.82 KK312 pKa = 8.12 QTACINFSWIRR323 pKa = 11.84 LILIALLIYY332 pKa = 9.71 FPIRR336 pKa = 11.84 WLVNKK341 pKa = 6.89 TTKK344 pKa = 10.24 PLFLWYY350 pKa = 10.16 DD351 pKa = 3.47 LMGLITYY358 pKa = 7.79 PVLLLINCLWKK369 pKa = 10.73 YY370 pKa = 10.8 FPLKK374 pKa = 10.56 CSNCGNLCIVTHH386 pKa = 6.83 EE387 pKa = 4.23 CTKK390 pKa = 10.65 VCICNKK396 pKa = 9.76 SKK398 pKa = 10.84 ASKK401 pKa = 9.28 EE402 pKa = 4.09 HH403 pKa = 6.89 SSEE406 pKa = 4.12 CPILSKK412 pKa = 10.84 EE413 pKa = 4.31 ADD415 pKa = 3.19 HH416 pKa = 7.78 DD417 pKa = 4.85 YY418 pKa = 11.53 NKK420 pKa = 10.73 HH421 pKa = 5.34 KK422 pKa = 7.35 WTSMEE427 pKa = 3.86 WFHH430 pKa = 7.65 LIVNTKK436 pKa = 10.66 LSLSLLKK443 pKa = 10.42 FVTEE447 pKa = 3.8 ILIGLVILSQMPMSMAQTTQCLSGCFYY474 pKa = 11.5 VPGCPFLVTSKK485 pKa = 10.89 FEE487 pKa = 4.07 KK488 pKa = 10.5 CSEE491 pKa = 4.27 KK492 pKa = 10.76 DD493 pKa = 3.04 QCYY496 pKa = 10.93 CNVKK500 pKa = 9.39 EE501 pKa = 4.39 DD502 pKa = 4.58 KK503 pKa = 10.43 IIEE506 pKa = 4.64 SIFGTNIVIEE516 pKa = 4.57 GPNDD520 pKa = 3.85 CIEE523 pKa = 4.21 NQNCIARR530 pKa = 11.84 PSIDD534 pKa = 3.83 NLIKK538 pKa = 10.66 CRR540 pKa = 11.84 LGCEE544 pKa = 3.97 YY545 pKa = 11.34 LDD547 pKa = 3.94 LFRR550 pKa = 11.84 NKK552 pKa = 9.74 PLYY555 pKa = 10.78 NGFSDD560 pKa = 3.99 YY561 pKa = 10.76 TGSSLGLTSVGLYY574 pKa = 6.99 EE575 pKa = 5.1 AKK577 pKa = 10.36 RR578 pKa = 11.84 LRR580 pKa = 11.84 NGIIDD585 pKa = 3.57 SYY587 pKa = 11.19 NRR589 pKa = 11.84 QGKK592 pKa = 9.0 ISGMVAGDD600 pKa = 3.75 SLNKK604 pKa = 10.31 NEE606 pKa = 4.13 TSIPEE611 pKa = 4.38 NILPRR616 pKa = 11.84 QSLIFDD622 pKa = 3.65 SVVDD626 pKa = 3.86 GKK628 pKa = 10.61 YY629 pKa = 10.2 RR630 pKa = 11.84 YY631 pKa = 9.37 MIEE634 pKa = 3.82 QSLLGGGGTIFMLNDD649 pKa = 3.37 KK650 pKa = 10.19 TSEE653 pKa = 4.02 TAKK656 pKa = 10.75 KK657 pKa = 9.2 FVIYY661 pKa = 9.85 IKK663 pKa = 10.68 SVGIHH668 pKa = 5.25 YY669 pKa = 7.95 EE670 pKa = 3.82 VSEE673 pKa = 4.82 KK674 pKa = 10.2 YY675 pKa = 6.08 TTAPIQSTHH684 pKa = 5.09 TDD686 pKa = 4.13 FYY688 pKa = 10.5 STCTGNCDD696 pKa = 3.29 TCRR699 pKa = 11.84 KK700 pKa = 8.9 NQALTGFQDD709 pKa = 3.98 FCVTPTSYY717 pKa = 9.64 WGCEE721 pKa = 3.99 EE722 pKa = 4.13 AWCFAINEE730 pKa = 4.33 GATCGFCRR738 pKa = 11.84 NIYY741 pKa = 11.16 DD742 pKa = 3.19 MDD744 pKa = 3.57 KK745 pKa = 10.63 SYY747 pKa = 11.13 RR748 pKa = 11.84 IYY750 pKa = 11.05 SVLKK754 pKa = 7.88 STIVADD760 pKa = 3.74 VCISGILGGQCSRR773 pKa = 11.84 ITEE776 pKa = 4.1 EE777 pKa = 3.99 VPYY780 pKa = 10.8 EE781 pKa = 4.0 NTLFQADD788 pKa = 3.65 IQADD792 pKa = 3.72 LHH794 pKa = 6.74 NDD796 pKa = 4.41 GITIGEE802 pKa = 4.79 LIAHH806 pKa = 6.91 GPDD809 pKa = 2.87 SHH811 pKa = 8.27 IYY813 pKa = 9.69 SGNIANLNDD822 pKa = 3.64 PVKK825 pKa = 10.45 MFGHH829 pKa = 6.71 PQLTHH834 pKa = 6.86 DD835 pKa = 4.72 GVPIFTKK842 pKa = 9.49 KK843 pKa = 8.73 TLEE846 pKa = 4.3 GDD848 pKa = 3.78 DD849 pKa = 4.42 MSWDD853 pKa = 3.46 CAAIGKK859 pKa = 10.08 KK860 pKa = 10.03 SVTIKK865 pKa = 9.08 TCGYY869 pKa = 7.19 DD870 pKa = 3.12 TYY872 pKa = 11.1 RR873 pKa = 11.84 FRR875 pKa = 11.84 SGLEE879 pKa = 3.83 QISDD883 pKa = 3.53 IPVSFKK889 pKa = 11.02 DD890 pKa = 4.1 FSSFFLAKK898 pKa = 10.23 SFSLGKK904 pKa = 10.49 LKK906 pKa = 10.28 MVVDD910 pKa = 5.16 LPSDD914 pKa = 4.11 LFKK917 pKa = 11.04 VAPKK921 pKa = 10.12 KK922 pKa = 10.48 PSITSTSLNCNGCLLCGQGLSCLLEE947 pKa = 4.18 FFSDD951 pKa = 3.84 LTFSTAISIDD961 pKa = 3.38 ACSLSTYY968 pKa = 10.34 QLAVKK973 pKa = 10.24 KK974 pKa = 10.77 GSNKK978 pKa = 9.93 YY979 pKa = 9.98 NITMFCSANPDD990 pKa = 3.51 KK991 pKa = 11.19 KK992 pKa = 11.22 KK993 pKa = 8.28 MTLYY997 pKa = 10.5 PEE999 pKa = 4.64 GNPDD1003 pKa = 2.79 ISVEE1007 pKa = 4.08 VLVNNVIVEE1016 pKa = 4.15 EE1017 pKa = 4.41 PEE1019 pKa = 4.43 NIIDD1023 pKa = 4.1 QNDD1026 pKa = 3.55 EE1027 pKa = 4.09 YY1028 pKa = 11.62 AHH1030 pKa = 7.07 EE1031 pKa = 4.17 EE1032 pKa = 3.71 QQYY1035 pKa = 11.29 NSDD1038 pKa = 3.28 SSAWGFWDD1046 pKa = 4.25 YY1047 pKa = 11.26 IKK1049 pKa = 11.24 SPFNFIASYY1058 pKa = 10.19 FGSFFDD1064 pKa = 4.18 TIRR1067 pKa = 11.84 VVLLIAFIFLVTYY1080 pKa = 8.97 FCSILTSICKK1090 pKa = 10.1 GYY1092 pKa = 10.85 VKK1094 pKa = 10.71 NEE1096 pKa = 4.1 SYY1098 pKa = 11.01 KK1099 pKa = 10.61 SRR1101 pKa = 11.84 SKK1103 pKa = 10.74 IEE1105 pKa = 5.15 DD1106 pKa = 3.66 DD1107 pKa = 4.89 DD1108 pKa = 4.19 EE1109 pKa = 5.95 PEE1111 pKa = 4.02 IKK1113 pKa = 10.62 APMLMKK1119 pKa = 10.14 DD1120 pKa = 2.78 TMTRR1124 pKa = 11.84 RR1125 pKa = 11.84 RR1126 pKa = 11.84 PPMDD1130 pKa = 3.92 FSHH1133 pKa = 6.98 LVV1135 pKa = 3.07
Molecular weight: 127.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.713
IPC2_protein 5.931
IPC_protein 5.957
Toseland 6.046
ProMoST 6.326
Dawson 6.249
Bjellqvist 6.287
Wikipedia 6.249
Rodwell 6.237
Grimsley 6.097
Solomon 6.249
Lehninger 6.249
Nozaki 6.547
DTASelect 6.678
Thurlkill 6.693
EMBOSS 6.649
Sillero 6.634
Patrickios 3.592
IPC_peptide 6.262
IPC2_peptide 6.517
IPC2.peptide.svr19 6.561
Protein with the highest isoelectric point:
>sp|P26002|NSS_TSWV1 Non-structural protein NS-S OS=Tomato spotted wilt virus (strain Brazilian Br-01) OX=36413 GN=NSS PE=3 SV=1
MM1 pKa = 7.46 SKK3 pKa = 10.67 VKK5 pKa = 9.03 LTKK8 pKa = 10.26 EE9 pKa = 4.24 SIVALLTQGKK19 pKa = 9.34 DD20 pKa = 3.27 LEE22 pKa = 4.41 FEE24 pKa = 4.0 EE25 pKa = 4.91 DD26 pKa = 3.4 QNLVAFNFKK35 pKa = 9.02 TFCLEE40 pKa = 4.82 NIDD43 pKa = 4.24 QIKK46 pKa = 9.75 KK47 pKa = 9.55 MSVISCLTFLKK58 pKa = 10.47 NRR60 pKa = 11.84 QSIMKK65 pKa = 9.42 VIKK68 pKa = 10.61 QSDD71 pKa = 3.68 FTFGKK76 pKa = 8.34 ITIKK80 pKa = 9.65 KK81 pKa = 7.41 TSDD84 pKa = 3.43 RR85 pKa = 11.84 IGGTDD90 pKa = 3.05 MTFRR94 pKa = 11.84 RR95 pKa = 11.84 LDD97 pKa = 3.1 SLIRR101 pKa = 11.84 VRR103 pKa = 11.84 LVEE106 pKa = 3.92 EE107 pKa = 4.2 TGNSEE112 pKa = 4.09 NLNTIKK118 pKa = 10.74 SKK120 pKa = 10.47 IASHH124 pKa = 6.64 PLIQAYY130 pKa = 9.08 GLPLDD135 pKa = 4.27 DD136 pKa = 4.82 AKK138 pKa = 10.88 SVRR141 pKa = 11.84 LAIMLGGSLPLIASVDD157 pKa = 3.86 SFEE160 pKa = 4.77 MISVVLAIYY169 pKa = 10.45 QDD171 pKa = 3.56 AKK173 pKa = 11.32 YY174 pKa = 9.92 KK175 pKa = 10.82 DD176 pKa = 3.89 LGIDD180 pKa = 3.32 PKK182 pKa = 11.13 KK183 pKa = 10.89 YY184 pKa = 8.9 DD185 pKa = 3.46 TKK187 pKa = 10.41 EE188 pKa = 3.85 ALGKK192 pKa = 10.3 VCTVLKK198 pKa = 10.78 SKK200 pKa = 10.62 AFEE203 pKa = 4.09 MNEE206 pKa = 3.92 DD207 pKa = 3.39 QVKK210 pKa = 10.35 KK211 pKa = 9.85 GKK213 pKa = 9.96 EE214 pKa = 3.56 YY215 pKa = 10.95 AAILSSSNPNAKK227 pKa = 9.99 GSVAMEE233 pKa = 4.65 HH234 pKa = 6.45 YY235 pKa = 10.83 SEE237 pKa = 4.38 TLNKK241 pKa = 9.16 FYY243 pKa = 11.18 EE244 pKa = 4.16 MFGVKK249 pKa = 9.56 KK250 pKa = 9.59 QAKK253 pKa = 8.86 LAEE256 pKa = 4.27 LAA258 pKa = 4.02
Molecular weight: 28.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.51
IPC2_protein 8.361
IPC_protein 8.244
Toseland 9.136
ProMoST 8.829
Dawson 9.37
Bjellqvist 9.092
Wikipedia 9.472
Rodwell 9.78
Grimsley 9.385
Solomon 9.443
Lehninger 9.414
Nozaki 9.311
DTASelect 9.033
Thurlkill 9.253
EMBOSS 9.56
Sillero 9.355
Patrickios 5.194
IPC_peptide 9.443
IPC2_peptide 7.585
IPC2.peptide.svr19 7.719
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
5033
258
2875
1006.6
114.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.093 ± 0.48
2.205 ± 0.597
5.682 ± 0.356
6.994 ± 0.737
4.391 ± 0.263
4.331 ± 0.525
1.669 ± 0.22
8.146 ± 0.257
8.782 ± 0.59
9.974 ± 0.533
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.782 ± 0.343
6.279 ± 0.44
3.04 ± 0.587
2.662 ± 0.359
3.715 ± 0.546
9.338 ± 0.578
5.762 ± 0.392
5.424 ± 0.379
0.854 ± 0.197
3.874 ± 0.356
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here