Kibdelosporangium aridum
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11140 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W1ZGX4|A0A1W1ZGX4_KIBAR Putative baseplate assembly protein OS=Kibdelosporangium aridum OX=2030 GN=SAMN05661093_00061 PE=4 SV=1
MM1 pKa = 7.12 STTFATFVYY10 pKa = 10.27 LLSTLSVDD18 pKa = 4.24 PSPKK22 pKa = 10.24 QKK24 pKa = 10.98 DD25 pKa = 3.34 KK26 pKa = 11.38 VSAHH30 pKa = 5.36 FRR32 pKa = 11.84 IQVSHH37 pKa = 5.41 MRR39 pKa = 11.84 TWFRR43 pKa = 11.84 TAGLVAVVCTPLMFVAGPGYY63 pKa = 10.7 AATEE67 pKa = 3.97 VDD69 pKa = 3.45 KK70 pKa = 11.41 SYY72 pKa = 8.28 TTAVLKK78 pKa = 9.78 NTDD81 pKa = 2.76 NRR83 pKa = 11.84 LNVLAGAGSAHH94 pKa = 7.93 DD95 pKa = 3.69 IRR97 pKa = 11.84 IDD99 pKa = 3.95 LVDD102 pKa = 3.54 NNYY105 pKa = 10.03 IVTDD109 pKa = 3.87 TGNLLITAAPCEE121 pKa = 4.1 RR122 pKa = 11.84 LTNPEE127 pKa = 4.13 RR128 pKa = 11.84 VSCPLPVSGITVEE141 pKa = 4.37 AGEE144 pKa = 5.0 LNDD147 pKa = 3.91 TVQLSDD153 pKa = 3.48 SVTVPSTLIGGAGNDD168 pKa = 3.97 RR169 pKa = 11.84 LDD171 pKa = 3.85 GGAGNDD177 pKa = 3.84 SLFGGAGNDD186 pKa = 3.78 GLLGGAGDD194 pKa = 4.54 DD195 pKa = 3.98 YY196 pKa = 11.87 LVGEE200 pKa = 4.99 AGNDD204 pKa = 3.45 ALLGGTDD211 pKa = 3.7 NDD213 pKa = 4.12 YY214 pKa = 11.49 LDD216 pKa = 4.37 SGVGNDD222 pKa = 3.77 YY223 pKa = 11.29 LFGGDD228 pKa = 4.71 GYY230 pKa = 11.41 DD231 pKa = 3.63 GLLGGAGDD239 pKa = 4.54 DD240 pKa = 3.98 YY241 pKa = 11.83 LVGEE245 pKa = 4.58 AGGDD249 pKa = 3.67 GLLGGDD255 pKa = 3.97 GNDD258 pKa = 3.77 YY259 pKa = 11.1 LDD261 pKa = 4.91 GGAEE265 pKa = 3.99 TDD267 pKa = 4.05 YY268 pKa = 11.69 LDD270 pKa = 4.73 GGAGNDD276 pKa = 3.33 RR277 pKa = 11.84 LFGSTGDD284 pKa = 4.16 DD285 pKa = 3.37 YY286 pKa = 11.83 LFSRR290 pKa = 11.84 DD291 pKa = 3.31 GGAGNDD297 pKa = 4.11 SVDD300 pKa = 3.9 GGPGTDD306 pKa = 3.6 TCDD309 pKa = 3.75 ADD311 pKa = 4.81 PGDD314 pKa = 4.08 TVINCPP320 pKa = 3.9
Molecular weight: 32.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.668
IPC2_protein 3.656
IPC_protein 3.706
Toseland 3.465
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.706
Rodwell 3.528
Grimsley 3.363
Solomon 3.719
Lehninger 3.681
Nozaki 3.834
DTASelect 4.164
Thurlkill 3.528
EMBOSS 3.706
Sillero 3.834
Patrickios 1.252
IPC_peptide 3.706
IPC2_peptide 3.808
IPC2.peptide.svr19 3.759
Protein with the highest isoelectric point:
>tr|A0A1W2DH51|A0A1W2DH51_KIBAR Uncharacterized protein OS=Kibdelosporangium aridum OX=2030 GN=SAMN05661093_03305 PE=4 SV=1
MM1 pKa = 7.53 SKK3 pKa = 10.53 GKK5 pKa = 8.66 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 AKK17 pKa = 8.7 THH19 pKa = 5.15 GFRR22 pKa = 11.84 LRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AILSARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 9.66 GRR42 pKa = 11.84 SEE44 pKa = 3.94 LSAA47 pKa = 4.73
Molecular weight: 5.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.455
IPC2_protein 10.979
IPC_protein 12.574
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.369
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.119
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11140
0
11140
3722341
39
6946
334.1
36.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.358 ± 0.032
0.797 ± 0.007
6.009 ± 0.02
5.307 ± 0.024
3.017 ± 0.014
8.677 ± 0.022
2.207 ± 0.012
4.03 ± 0.018
2.489 ± 0.019
10.179 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.952 ± 0.01
2.366 ± 0.016
5.736 ± 0.023
3.233 ± 0.013
7.393 ± 0.023
5.46 ± 0.016
6.239 ± 0.022
8.782 ± 0.022
1.605 ± 0.009
2.162 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here