Alistipes sp. An66

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Rikenellaceae; Alistipes; unclassified Alistipes

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2381 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y3VGZ0|A0A1Y3VGZ0_9BACT V-type ATP synthase subunit D OS=Alistipes sp. An66 OX=1965650 GN=B5G16_02905 PE=3 SV=1
MM1 pKa = 7.61KK2 pKa = 10.47KK3 pKa = 10.33FLFLGWLLAAASLTACDD20 pKa = 4.72KK21 pKa = 11.44DD22 pKa = 3.83NEE24 pKa = 4.47QEE26 pKa = 4.96DD27 pKa = 5.4GPCPVTDD34 pKa = 3.45IEE36 pKa = 4.89LPQSSEE42 pKa = 3.73QTPIEE47 pKa = 4.49PGSTIEE53 pKa = 3.65IRR55 pKa = 11.84GNGFEE60 pKa = 4.89DD61 pKa = 4.53DD62 pKa = 3.95CEE64 pKa = 3.85IWLFGASQTAGVQAEE79 pKa = 4.17ILEE82 pKa = 4.47VSASAVRR89 pKa = 11.84FTAPNLCGTQRR100 pKa = 11.84IEE102 pKa = 3.61LRR104 pKa = 11.84QNGGVWDD111 pKa = 3.62IGTLVFPEE119 pKa = 4.43EE120 pKa = 3.95EE121 pKa = 4.06MLPIEE126 pKa = 4.16ILPRR130 pKa = 11.84RR131 pKa = 11.84ISHH134 pKa = 6.63IRR136 pKa = 11.84ITYY139 pKa = 9.66PDD141 pKa = 4.43DD142 pKa = 3.95LSFVQQFAYY151 pKa = 10.51DD152 pKa = 3.67EE153 pKa = 4.17QGRR156 pKa = 11.84IASITEE162 pKa = 3.49TDD164 pKa = 3.01QQYY167 pKa = 11.1AYY169 pKa = 9.74PGTTAQDD176 pKa = 3.28EE177 pKa = 4.73VTEE180 pKa = 4.09ITYY183 pKa = 10.52EE184 pKa = 3.78SDD186 pKa = 3.15RR187 pKa = 11.84IVITEE192 pKa = 3.82PSGAQSILTLADD204 pKa = 3.15GRR206 pKa = 11.84ATAITGVAFNDD217 pKa = 4.03NYY219 pKa = 10.53PDD221 pKa = 4.93DD222 pKa = 3.8YY223 pKa = 11.84AFTYY227 pKa = 10.09DD228 pKa = 3.35ANGYY232 pKa = 9.14IATSTWTQNPDD243 pKa = 2.63KK244 pKa = 10.18TYY246 pKa = 10.54SAKK249 pKa = 10.49SLYY252 pKa = 9.82TVVNGCMTQVRR263 pKa = 11.84EE264 pKa = 4.4AEE266 pKa = 5.27DD267 pKa = 3.19SGEE270 pKa = 4.09WYY272 pKa = 10.47GGNASYY278 pKa = 11.01TNNPEE283 pKa = 3.95RR284 pKa = 11.84MNNLNIDD291 pKa = 3.91LFGISDD297 pKa = 5.36FITTTDD303 pKa = 3.32LDD305 pKa = 4.0RR306 pKa = 11.84IYY308 pKa = 11.16LVGAGGNRR316 pKa = 11.84LKK318 pKa = 10.78KK319 pKa = 10.59LPASVDD325 pKa = 3.44YY326 pKa = 11.33GEE328 pKa = 5.25NGVDD332 pKa = 3.27TYY334 pKa = 11.02RR335 pKa = 11.84YY336 pKa = 7.75TMEE339 pKa = 4.49GEE341 pKa = 4.41YY342 pKa = 10.0ISKK345 pKa = 10.21IEE347 pKa = 3.89IDD349 pKa = 3.79EE350 pKa = 4.83DD351 pKa = 4.37GEE353 pKa = 4.19PLTILEE359 pKa = 4.6IFYY362 pKa = 11.07EE363 pKa = 4.41DD364 pKa = 3.2

Molecular weight: 40.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y3V9U3|A0A1Y3V9U3_9BACT NAD metabolism ATPase/kinase OS=Alistipes sp. An66 OX=1965650 GN=B5G16_11420 PE=4 SV=1
MM1 pKa = 7.27FKK3 pKa = 10.74KK4 pKa = 10.48RR5 pKa = 11.84MLIIPNLLMSLQRR18 pKa = 11.84SLKK21 pKa = 7.86TRR23 pKa = 11.84LWLVFVLMSFTRR35 pKa = 11.84SLILIIFLMLFMIWVIFLTSLLIFLLNWRR64 pKa = 11.84NIWW67 pKa = 3.32

Molecular weight: 8.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2381

0

2381

895525

38

1604

376.1

42.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.094 ± 0.055

1.279 ± 0.018

5.715 ± 0.034

6.686 ± 0.05

4.19 ± 0.028

7.61 ± 0.038

1.806 ± 0.022

5.722 ± 0.041

4.289 ± 0.039

9.218 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.446 ± 0.023

3.912 ± 0.036

4.263 ± 0.028

3.069 ± 0.023

6.841 ± 0.054

5.753 ± 0.041

5.842 ± 0.044

6.92 ± 0.038

1.388 ± 0.02

3.958 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski