Vibrio phage pVa-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V1FCA5|A0A1V1FCA5_9CAUD Uncharacterized protein OS=Vibrio phage pVa-1 OX=1897751 GN=pVa1_0004 PE=4 SV=1
MM1 pKa = 7.15FHH3 pKa = 6.9TSPKK7 pKa = 9.89AIEE10 pKa = 4.0IGTINKK16 pKa = 9.85YY17 pKa = 10.16GVAGDD22 pKa = 4.4CLFFSDD28 pKa = 5.83DD29 pKa = 3.73IYY31 pKa = 11.26IMTASRR37 pKa = 11.84TCYY40 pKa = 10.29VYY42 pKa = 10.58EE43 pKa = 5.15ADD45 pKa = 4.1FDD47 pKa = 4.47CVRR50 pKa = 11.84ASQLYY55 pKa = 9.96DD56 pKa = 3.54DD57 pKa = 5.78EE58 pKa = 6.77IIADD62 pKa = 3.57IARR65 pKa = 11.84YY66 pKa = 9.43FDD68 pKa = 3.66VDD70 pKa = 3.16EE71 pKa = 4.6STAEE75 pKa = 4.03SLLDD79 pKa = 3.35GTQNEE84 pKa = 4.29WCLDD88 pKa = 3.67DD89 pKa = 5.49FDD91 pKa = 6.54CEE93 pKa = 5.18GEE95 pKa = 4.17NSWWLQGKK103 pKa = 9.96RR104 pKa = 11.84GEE106 pKa = 4.64CAVKK110 pKa = 9.78MGYY113 pKa = 9.76DD114 pKa = 3.25GCEE117 pKa = 4.25DD118 pKa = 3.79EE119 pKa = 6.04DD120 pKa = 4.1EE121 pKa = 4.34QGTVYY126 pKa = 10.25IVPMAGRR133 pKa = 11.84EE134 pKa = 4.22SEE136 pKa = 4.29LKK138 pKa = 10.7LVEE141 pKa = 4.43KK142 pKa = 10.58KK143 pKa = 10.61

Molecular weight: 16.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U9GMQ0|A0A1U9GMQ0_9CAUD Uncharacterized protein OS=Vibrio phage pVa-1 OX=1897751 GN=pVa1_0045 PE=4 SV=1
MM1 pKa = 7.32SCAIKK6 pKa = 9.8TKK8 pKa = 10.24NDD10 pKa = 3.68NIHH13 pKa = 5.93TPRR16 pKa = 11.84DD17 pKa = 3.38FRR19 pKa = 11.84GPRR22 pKa = 11.84NVFVNGNKK30 pKa = 9.64IKK32 pKa = 10.48FVVYY36 pKa = 10.76ADD38 pKa = 3.63TKK40 pKa = 10.11KK41 pKa = 11.1GIVKK45 pKa = 7.46YY46 pKa = 9.88HH47 pKa = 5.54PHH49 pKa = 5.85PVRR52 pKa = 11.84RR53 pKa = 11.84KK54 pKa = 7.87RR55 pKa = 11.84GSNVVYY61 pKa = 10.12TRR63 pKa = 11.84TLKK66 pKa = 11.14GLVTVEE72 pKa = 5.25FIRR75 pKa = 5.3

Molecular weight: 8.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

16280

37

1176

180.9

20.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.967 ± 0.421

1.394 ± 0.173

5.86 ± 0.251

6.572 ± 0.235

3.649 ± 0.202

7.039 ± 0.258

1.769 ± 0.187

6.658 ± 0.179

6.757 ± 0.336

7.703 ± 0.312

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.764 ± 0.141

5.117 ± 0.189

3.802 ± 0.273

4.091 ± 0.281

4.662 ± 0.2

7.746 ± 0.257

5.418 ± 0.266

6.186 ± 0.284

1.505 ± 0.106

3.342 ± 0.203
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski