Amino acid dipepetide frequency for Vibrio phage pVa-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.757AlaAla: 6.757 ± 0.895
0.614AlaCys: 0.614 ± 0.204
5.283AlaAsp: 5.283 ± 0.591
4.177AlaGlu: 4.177 ± 0.563
2.826AlaPhe: 2.826 ± 0.425
5.529AlaGly: 5.529 ± 0.736
0.983AlaHis: 0.983 ± 0.228
6.757AlaIle: 6.757 ± 0.828
6.696AlaLys: 6.696 ± 0.64
6.573AlaLeu: 6.573 ± 0.695
3.071AlaMet: 3.071 ± 0.497
3.993AlaAsn: 3.993 ± 0.364
2.089AlaPro: 2.089 ± 0.374
3.071AlaGln: 3.071 ± 0.492
3.133AlaArg: 3.133 ± 0.432
5.774AlaSer: 5.774 ± 0.915
3.931AlaThr: 3.931 ± 0.451
4.73AlaVal: 4.73 ± 0.527
1.167AlaTrp: 1.167 ± 0.247
2.519AlaTyr: 2.519 ± 0.386
0.0AlaXaa: 0.0 ± 0.0
Cys
0.614CysAla: 0.614 ± 0.207
0.43CysCys: 0.43 ± 0.173
1.106CysAsp: 1.106 ± 0.306
1.106CysGlu: 1.106 ± 0.287
0.246CysPhe: 0.246 ± 0.13
1.167CysGly: 1.167 ± 0.331
0.43CysHis: 0.43 ± 0.185
0.614CysIle: 0.614 ± 0.175
0.614CysLys: 0.614 ± 0.209
0.983CysLeu: 0.983 ± 0.276
0.43CysMet: 0.43 ± 0.152
0.43CysAsn: 0.43 ± 0.173
0.246CysPro: 0.246 ± 0.132
0.43CysGln: 0.43 ± 0.175
0.86CysArg: 0.86 ± 0.244
1.044CysSer: 1.044 ± 0.265
1.044CysThr: 1.044 ± 0.244
0.86CysVal: 0.86 ± 0.282
0.614CysTrp: 0.614 ± 0.218
0.676CysTyr: 0.676 ± 0.206
0.0CysXaa: 0.0 ± 0.0
Asp
4.546AspAla: 4.546 ± 0.608
0.86AspCys: 0.86 ± 0.317
4.054AspAsp: 4.054 ± 0.505
4.177AspGlu: 4.177 ± 0.686
2.764AspPhe: 2.764 ± 0.416
5.406AspGly: 5.406 ± 0.636
0.86AspHis: 0.86 ± 0.239
3.809AspIle: 3.809 ± 0.426
3.379AspLys: 3.379 ± 0.461
4.177AspLeu: 4.177 ± 0.435
1.413AspMet: 1.413 ± 0.265
2.887AspAsn: 2.887 ± 0.452
2.15AspPro: 2.15 ± 0.375
2.334AspGln: 2.334 ± 0.484
2.641AspArg: 2.641 ± 0.383
3.993AspSer: 3.993 ± 0.586
2.641AspThr: 2.641 ± 0.433
2.764AspVal: 2.764 ± 0.421
1.29AspTrp: 1.29 ± 0.241
2.457AspTyr: 2.457 ± 0.364
0.0AspXaa: 0.0 ± 0.0
Glu
4.546GluAla: 4.546 ± 0.698
0.799GluCys: 0.799 ± 0.246
2.887GluAsp: 2.887 ± 0.408
3.686GluGlu: 3.686 ± 0.567
3.071GluPhe: 3.071 ± 0.426
3.133GluGly: 3.133 ± 0.399
1.351GluHis: 1.351 ± 0.329
4.976GluIle: 4.976 ± 0.591
3.624GluLys: 3.624 ± 0.579
7.187GluLeu: 7.187 ± 0.738
2.58GluMet: 2.58 ± 0.47
3.133GluAsn: 3.133 ± 0.487
2.826GluPro: 2.826 ± 0.856
4.116GluGln: 4.116 ± 0.663
3.01GluArg: 3.01 ± 0.441
5.959GluSer: 5.959 ± 0.584
2.15GluThr: 2.15 ± 0.332
3.993GluVal: 3.993 ± 0.616
1.106GluTrp: 1.106 ± 0.261
1.597GluTyr: 1.597 ± 0.258
0.0GluXaa: 0.0 ± 0.0
Phe
2.641PheAla: 2.641 ± 0.389
0.491PheCys: 0.491 ± 0.165
2.887PheAsp: 2.887 ± 0.474
2.273PheGlu: 2.273 ± 0.35
1.29PhePhe: 1.29 ± 0.298
3.256PheGly: 3.256 ± 0.569
0.491PheHis: 0.491 ± 0.169
1.72PheIle: 1.72 ± 0.396
2.826PheLys: 2.826 ± 0.404
2.887PheLeu: 2.887 ± 0.494
0.737PheMet: 0.737 ± 0.221
2.027PheAsn: 2.027 ± 0.322
1.106PhePro: 1.106 ± 0.268
0.799PheGln: 0.799 ± 0.197
1.843PheArg: 1.843 ± 0.362
2.764PheSer: 2.764 ± 0.385
2.519PheThr: 2.519 ± 0.41
2.15PheVal: 2.15 ± 0.355
0.614PheTrp: 0.614 ± 0.215
1.167PheTyr: 1.167 ± 0.291
0.0PheXaa: 0.0 ± 0.0
Gly
5.897GlyAla: 5.897 ± 0.758
0.983GlyCys: 0.983 ± 0.257
3.809GlyAsp: 3.809 ± 0.644
4.239GlyGlu: 4.239 ± 0.486
2.703GlyPhe: 2.703 ± 0.403
5.897GlyGly: 5.897 ± 0.752
0.921GlyHis: 0.921 ± 0.247
4.853GlyIle: 4.853 ± 0.702
4.3GlyLys: 4.3 ± 0.593
5.283GlyLeu: 5.283 ± 0.625
2.027GlyMet: 2.027 ± 0.393
3.194GlyAsn: 3.194 ± 0.438
1.229GlyPro: 1.229 ± 0.241
2.396GlyGln: 2.396 ± 0.361
2.764GlyArg: 2.764 ± 0.33
4.976GlySer: 4.976 ± 0.6
4.607GlyThr: 4.607 ± 0.583
6.081GlyVal: 6.081 ± 0.664
1.413GlyTrp: 1.413 ± 0.267
2.826GlyTyr: 2.826 ± 0.471
0.0GlyXaa: 0.0 ± 0.0
His
1.106HisAla: 1.106 ± 0.34
0.553HisCys: 0.553 ± 0.18
1.167HisAsp: 1.167 ± 0.322
1.29HisGlu: 1.29 ± 0.38
0.737HisPhe: 0.737 ± 0.207
1.106HisGly: 1.106 ± 0.291
0.737HisHis: 0.737 ± 0.344
1.106HisIle: 1.106 ± 0.24
1.167HisLys: 1.167 ± 0.282
1.413HisLeu: 1.413 ± 0.293
0.43HisMet: 0.43 ± 0.251
0.614HisAsn: 0.614 ± 0.197
0.921HisPro: 0.921 ± 0.283
0.799HisGln: 0.799 ± 0.216
0.86HisArg: 0.86 ± 0.345
0.86HisSer: 0.86 ± 0.25
0.86HisThr: 0.86 ± 0.236
1.29HisVal: 1.29 ± 0.283
0.307HisTrp: 0.307 ± 0.128
0.369HisTyr: 0.369 ± 0.154
0.0HisXaa: 0.0 ± 0.0
Ile
6.45IleAla: 6.45 ± 0.753
1.229IleCys: 1.229 ± 0.287
4.3IleAsp: 4.3 ± 0.637
5.221IleGlu: 5.221 ± 0.573
1.72IlePhe: 1.72 ± 0.343
5.221IleGly: 5.221 ± 0.677
0.983IleHis: 0.983 ± 0.272
3.194IleIle: 3.194 ± 0.424
5.713IleLys: 5.713 ± 0.606
3.379IleLeu: 3.379 ± 0.439
0.983IleMet: 0.983 ± 0.268
4.3IleAsn: 4.3 ± 0.542
2.396IlePro: 2.396 ± 0.364
2.273IleGln: 2.273 ± 0.453
2.887IleArg: 2.887 ± 0.429
6.143IleSer: 6.143 ± 0.74
3.624IleThr: 3.624 ± 0.374
3.501IleVal: 3.501 ± 0.448
0.676IleTrp: 0.676 ± 0.185
2.396IleTyr: 2.396 ± 0.349
0.0IleXaa: 0.0 ± 0.0
Lys
7.31LysAla: 7.31 ± 1.0
1.106LysCys: 1.106 ± 0.332
2.949LysAsp: 2.949 ± 0.47
4.976LysGlu: 4.976 ± 0.638
2.519LysPhe: 2.519 ± 0.432
4.054LysGly: 4.054 ± 0.51
1.29LysHis: 1.29 ± 0.279
3.01LysIle: 3.01 ± 0.402
4.73LysLys: 4.73 ± 0.781
5.037LysLeu: 5.037 ± 0.595
1.904LysMet: 1.904 ± 0.359
3.071LysAsn: 3.071 ± 0.382
3.379LysPro: 3.379 ± 0.584
3.501LysGln: 3.501 ± 0.549
3.747LysArg: 3.747 ± 0.654
4.976LysSer: 4.976 ± 0.545
3.931LysThr: 3.931 ± 0.452
3.317LysVal: 3.317 ± 0.583
0.921LysTrp: 0.921 ± 0.244
1.843LysTyr: 1.843 ± 0.321
0.0LysXaa: 0.0 ± 0.0
Leu
6.143LeuAla: 6.143 ± 0.718
0.983LeuCys: 0.983 ± 0.252
4.3LeuAsp: 4.3 ± 0.441
5.467LeuGlu: 5.467 ± 0.623
2.089LeuPhe: 2.089 ± 0.342
4.054LeuGly: 4.054 ± 0.502
1.474LeuHis: 1.474 ± 0.311
5.529LeuIle: 5.529 ± 0.604
5.467LeuLys: 5.467 ± 0.585
5.16LeuLeu: 5.16 ± 0.49
2.211LeuMet: 2.211 ± 0.376
4.239LeuAsn: 4.239 ± 0.646
3.317LeuPro: 3.317 ± 0.541
2.58LeuGln: 2.58 ± 0.496
3.44LeuArg: 3.44 ± 0.474
6.634LeuSer: 6.634 ± 0.708
5.037LeuThr: 5.037 ± 0.467
5.221LeuVal: 5.221 ± 0.725
1.044LeuTrp: 1.044 ± 0.206
2.641LeuTyr: 2.641 ± 0.377
0.0LeuXaa: 0.0 ± 0.0
Met
2.211MetAla: 2.211 ± 0.356
0.246MetCys: 0.246 ± 0.116
1.29MetAsp: 1.29 ± 0.293
1.597MetGlu: 1.597 ± 0.356
0.86MetPhe: 0.86 ± 0.258
0.983MetGly: 0.983 ± 0.241
0.369MetHis: 0.369 ± 0.158
1.781MetIle: 1.781 ± 0.364
2.396MetLys: 2.396 ± 0.503
2.334MetLeu: 2.334 ± 0.381
0.737MetMet: 0.737 ± 0.229
1.659MetAsn: 1.659 ± 0.342
1.659MetPro: 1.659 ± 0.311
1.351MetGln: 1.351 ± 0.26
1.229MetArg: 1.229 ± 0.247
2.949MetSer: 2.949 ± 0.416
1.536MetThr: 1.536 ± 0.356
1.597MetVal: 1.597 ± 0.284
0.43MetTrp: 0.43 ± 0.187
0.43MetTyr: 0.43 ± 0.14
0.0MetXaa: 0.0 ± 0.0
Asn
4.423AsnAla: 4.423 ± 0.568
0.737AsnCys: 0.737 ± 0.287
2.764AsnAsp: 2.764 ± 0.495
2.764AsnGlu: 2.764 ± 0.368
1.351AsnPhe: 1.351 ± 0.252
4.607AsnGly: 4.607 ± 0.533
1.351AsnHis: 1.351 ± 0.339
2.949AsnIle: 2.949 ± 0.458
4.054AsnLys: 4.054 ± 0.527
3.256AsnLeu: 3.256 ± 0.507
1.413AsnMet: 1.413 ± 0.244
3.501AsnAsn: 3.501 ± 0.569
2.089AsnPro: 2.089 ± 0.368
2.519AsnGln: 2.519 ± 0.382
2.703AsnArg: 2.703 ± 0.489
2.764AsnSer: 2.764 ± 0.425
1.781AsnThr: 1.781 ± 0.33
3.563AsnVal: 3.563 ± 0.459
0.921AsnTrp: 0.921 ± 0.209
1.659AsnTyr: 1.659 ± 0.296
0.0AsnXaa: 0.0 ± 0.0
Pro
3.071ProAla: 3.071 ± 0.399
0.614ProCys: 0.614 ± 0.191
2.334ProAsp: 2.334 ± 0.367
3.87ProGlu: 3.87 ± 0.757
1.167ProPhe: 1.167 ± 0.191
1.536ProGly: 1.536 ± 0.312
0.799ProHis: 0.799 ± 0.253
2.211ProIle: 2.211 ± 0.46
1.843ProLys: 1.843 ± 0.429
2.703ProLeu: 2.703 ± 0.507
1.351ProMet: 1.351 ± 0.294
1.72ProAsn: 1.72 ± 0.326
1.536ProPro: 1.536 ± 0.386
1.413ProGln: 1.413 ± 0.312
1.659ProArg: 1.659 ± 0.368
3.931ProSer: 3.931 ± 0.536
2.457ProThr: 2.457 ± 0.419
2.396ProVal: 2.396 ± 0.331
0.307ProTrp: 0.307 ± 0.113
1.106ProTyr: 1.106 ± 0.247
0.0ProXaa: 0.0 ± 0.0
Gln
3.317GlnAla: 3.317 ± 0.532
0.369GlnCys: 0.369 ± 0.154
1.351GlnAsp: 1.351 ± 0.269
2.58GlnGlu: 2.58 ± 0.471
1.843GlnPhe: 1.843 ± 0.322
2.089GlnGly: 2.089 ± 0.39
0.491GlnHis: 0.491 ± 0.163
3.01GlnIle: 3.01 ± 0.555
2.396GlnLys: 2.396 ± 0.374
4.484GlnLeu: 4.484 ± 0.573
1.536GlnMet: 1.536 ± 0.332
2.089GlnAsn: 2.089 ± 0.401
1.536GlnPro: 1.536 ± 0.372
2.519GlnGln: 2.519 ± 0.68
1.72GlnArg: 1.72 ± 0.352
3.317GlnSer: 3.317 ± 0.432
1.781GlnThr: 1.781 ± 0.294
2.089GlnVal: 2.089 ± 0.346
0.491GlnTrp: 0.491 ± 0.139
1.904GlnTyr: 1.904 ± 0.341
0.0GlnXaa: 0.0 ± 0.0
Arg
3.317ArgAla: 3.317 ± 0.404
0.123ArgCys: 0.123 ± 0.093
2.334ArgAsp: 2.334 ± 0.292
3.256ArgGlu: 3.256 ± 0.486
2.334ArgPhe: 2.334 ± 0.431
2.273ArgGly: 2.273 ± 0.359
0.86ArgHis: 0.86 ± 0.252
4.3ArgIle: 4.3 ± 0.526
3.44ArgLys: 3.44 ± 0.512
4.853ArgLeu: 4.853 ± 0.566
1.597ArgMet: 1.597 ± 0.317
2.15ArgAsn: 2.15 ± 0.404
1.351ArgPro: 1.351 ± 0.401
1.229ArgGln: 1.229 ± 0.346
2.396ArgArg: 2.396 ± 0.46
3.686ArgSer: 3.686 ± 0.446
2.211ArgThr: 2.211 ± 0.356
2.703ArgVal: 2.703 ± 0.527
0.43ArgTrp: 0.43 ± 0.145
1.72ArgTyr: 1.72 ± 0.423
0.0ArgXaa: 0.0 ± 0.0
Ser
5.959SerAla: 5.959 ± 0.829
0.983SerCys: 0.983 ± 0.317
4.423SerAsp: 4.423 ± 0.617
5.529SerGlu: 5.529 ± 0.43
3.624SerPhe: 3.624 ± 0.479
7.31SerGly: 7.31 ± 0.743
1.044SerHis: 1.044 ± 0.221
5.959SerIle: 5.959 ± 0.839
4.607SerLys: 4.607 ± 0.577
5.221SerLeu: 5.221 ± 0.593
1.351SerMet: 1.351 ± 0.263
3.993SerAsn: 3.993 ± 0.474
3.01SerPro: 3.01 ± 0.511
3.133SerGln: 3.133 ± 0.518
3.686SerArg: 3.686 ± 0.551
5.59SerSer: 5.59 ± 0.776
3.686SerThr: 3.686 ± 0.533
4.73SerVal: 4.73 ± 0.581
0.737SerTrp: 0.737 ± 0.237
2.887SerTyr: 2.887 ± 0.387
0.0SerXaa: 0.0 ± 0.0
Thr
4.239ThrAla: 4.239 ± 0.532
0.676ThrCys: 0.676 ± 0.235
3.686ThrAsp: 3.686 ± 0.475
2.519ThrGlu: 2.519 ± 0.345
1.781ThrPhe: 1.781 ± 0.479
5.344ThrGly: 5.344 ± 0.502
0.983ThrHis: 0.983 ± 0.259
3.686ThrIle: 3.686 ± 0.502
3.01ThrLys: 3.01 ± 0.52
4.607ThrLeu: 4.607 ± 0.554
1.351ThrMet: 1.351 ± 0.317
2.641ThrAsn: 2.641 ± 0.484
2.826ThrPro: 2.826 ± 0.493
2.273ThrGln: 2.273 ± 0.382
2.15ThrArg: 2.15 ± 0.451
3.317ThrSer: 3.317 ± 0.594
2.826ThrThr: 2.826 ± 0.57
3.931ThrVal: 3.931 ± 0.686
0.553ThrTrp: 0.553 ± 0.195
1.781ThrTyr: 1.781 ± 0.38
0.0ThrXaa: 0.0 ± 0.0
Val
4.116ValAla: 4.116 ± 0.607
0.737ValCys: 0.737 ± 0.235
4.791ValAsp: 4.791 ± 0.591
4.054ValGlu: 4.054 ± 0.556
1.904ValPhe: 1.904 ± 0.308
4.669ValGly: 4.669 ± 0.579
1.106ValHis: 1.106 ± 0.272
3.686ValIle: 3.686 ± 0.504
4.73ValLys: 4.73 ± 0.553
3.624ValLeu: 3.624 ± 0.398
1.597ValMet: 1.597 ± 0.313
3.563ValAsn: 3.563 ± 0.396
2.334ValPro: 2.334 ± 0.378
1.659ValGln: 1.659 ± 0.352
3.194ValArg: 3.194 ± 0.438
4.669ValSer: 4.669 ± 0.533
4.484ValThr: 4.484 ± 0.576
3.747ValVal: 3.747 ± 0.509
0.921ValTrp: 0.921 ± 0.236
2.273ValTyr: 2.273 ± 0.351
0.0ValXaa: 0.0 ± 0.0
Trp
0.921TrpAla: 0.921 ± 0.222
0.43TrpCys: 0.43 ± 0.161
0.737TrpAsp: 0.737 ± 0.236
1.229TrpGlu: 1.229 ± 0.308
0.553TrpPhe: 0.553 ± 0.197
0.676TrpGly: 0.676 ± 0.238
0.369TrpHis: 0.369 ± 0.198
0.921TrpIle: 0.921 ± 0.248
0.983TrpLys: 0.983 ± 0.269
1.351TrpLeu: 1.351 ± 0.285
0.184TrpMet: 0.184 ± 0.097
0.614TrpAsn: 0.614 ± 0.211
0.737TrpPro: 0.737 ± 0.19
0.86TrpGln: 0.86 ± 0.193
0.799TrpArg: 0.799 ± 0.16
0.921TrpSer: 0.921 ± 0.245
0.86TrpThr: 0.86 ± 0.258
0.983TrpVal: 0.983 ± 0.262
0.491TrpTrp: 0.491 ± 0.21
0.43TrpTyr: 0.43 ± 0.164
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.089TyrAla: 2.089 ± 0.349
0.983TyrCys: 0.983 ± 0.24
2.15TyrAsp: 2.15 ± 0.394
1.72TyrGlu: 1.72 ± 0.352
1.106TyrPhe: 1.106 ± 0.258
2.089TyrGly: 2.089 ± 0.391
0.799TyrHis: 0.799 ± 0.248
2.519TyrIle: 2.519 ± 0.373
1.597TyrLys: 1.597 ± 0.267
2.519TyrLeu: 2.519 ± 0.388
0.676TyrMet: 0.676 ± 0.256
1.351TyrAsn: 1.351 ± 0.276
1.29TyrPro: 1.29 ± 0.281
1.659TyrGln: 1.659 ± 0.282
1.904TyrArg: 1.904 ± 0.37
3.194TyrSer: 3.194 ± 0.446
2.211TyrThr: 2.211 ± 0.404
2.211TyrVal: 2.211 ± 0.464
0.614TyrTrp: 0.614 ± 0.191
0.737TyrTyr: 0.737 ± 0.208
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 90 proteins (16280 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski