Bacillus sp. AFS040349

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5011 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2C1KAW4|A0A2C1KAW4_9BACI G-D-S-L family lipolytic protein OS=Bacillus sp. AFS040349 OX=2033502 GN=COD11_14085 PE=4 SV=1
MM1 pKa = 7.37YY2 pKa = 10.45LPIIEE7 pKa = 4.97CYY9 pKa = 9.75MIIFLFIEE17 pKa = 4.47SYY19 pKa = 10.22VIQRR23 pKa = 11.84VAEE26 pKa = 4.12LEE28 pKa = 3.88MDD30 pKa = 3.44VTLGYY35 pKa = 10.85FEE37 pKa = 4.39MVEE40 pKa = 4.04NLEE43 pKa = 4.24VDD45 pKa = 3.69CSSKK49 pKa = 10.82QEE51 pKa = 4.16EE52 pKa = 4.33FTLLAFSEE60 pKa = 4.71NEE62 pKa = 3.96DD63 pKa = 3.68CSTSEE68 pKa = 3.72VSGPAIVPTISVLDD82 pKa = 4.37DD83 pKa = 3.85GALEE87 pKa = 4.27LMNSLEE93 pKa = 4.09QEE95 pKa = 4.26VMEE98 pKa = 5.29LNQGDD103 pKa = 4.13NLDD106 pKa = 3.91NQEE109 pKa = 4.34DD110 pKa = 5.72YY111 pKa = 11.31YY112 pKa = 11.53VYY114 pKa = 11.03SDD116 pKa = 4.13ADD118 pKa = 4.07CPPEE122 pKa = 5.89LDD124 pKa = 3.69GQNDD128 pKa = 3.15EE129 pKa = 4.5SQWVKK134 pKa = 10.79LNSVEE139 pKa = 4.33EE140 pKa = 4.24EE141 pKa = 3.68EE142 pKa = 5.82DD143 pKa = 3.82YY144 pKa = 11.74LSMSDD149 pKa = 4.71LNGEE153 pKa = 4.54NSDD156 pKa = 3.47QQLNIDD162 pKa = 4.46SYY164 pKa = 11.32SQFSTSKK171 pKa = 10.38ISDD174 pKa = 3.4NMEE177 pKa = 4.15GLQQWTVKK185 pKa = 9.98VVGMEE190 pKa = 3.72DD191 pKa = 3.64AYY193 pKa = 10.3IHH195 pKa = 6.49VSDD198 pKa = 5.21GQRR201 pKa = 11.84IWLNVGIEE209 pKa = 4.09KK210 pKa = 10.45ARR212 pKa = 11.84KK213 pKa = 7.44ITNNDD218 pKa = 2.46ILAIDD223 pKa = 3.51VMKK226 pKa = 10.72NGKK229 pKa = 7.91EE230 pKa = 4.02VEE232 pKa = 4.05VLRR235 pKa = 11.84IIKK238 pKa = 10.24LEE240 pKa = 4.06SGVNEE245 pKa = 5.08DD246 pKa = 3.69YY247 pKa = 11.18LIPDD251 pKa = 3.56EE252 pKa = 4.31EE253 pKa = 5.6SYY255 pKa = 11.18FGVEE259 pKa = 4.09DD260 pKa = 3.91SKK262 pKa = 11.99VAMM265 pKa = 4.85

Molecular weight: 30.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2C1KAB3|A0A2C1KAB3_9BACI MarR family transcriptional regulator OS=Bacillus sp. AFS040349 OX=2033502 GN=COD11_15180 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 9.59VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.48VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.94VLSAA44 pKa = 4.11

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5011

0

5011

1419903

18

1878

283.4

31.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.273 ± 0.035

0.736 ± 0.011

5.087 ± 0.028

7.711 ± 0.04

4.595 ± 0.027

6.511 ± 0.034

2.092 ± 0.017

8.119 ± 0.045

7.271 ± 0.031

9.806 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.658 ± 0.017

4.777 ± 0.028

3.403 ± 0.022

3.828 ± 0.029

3.756 ± 0.028

6.381 ± 0.029

5.485 ± 0.024

6.909 ± 0.03

0.971 ± 0.013

3.63 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski