Amino acid dipepetide frequency for Bacillus sp. AFS040349

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.732AlaAla: 4.732 ± 0.076	0.54AlaCys: 0.54 ± 0.02	2.923AlaAsp: 2.923 ± 0.046	4.214AlaGlu: 4.214 ± 0.061	2.976AlaPhe: 2.976 ± 0.047	4.545AlaGly: 4.545 ± 0.063	1.145AlaHis: 1.145 ± 0.03	5.638AlaIle: 5.638 ± 0.069	4.488AlaLys: 4.488 ± 0.072	6.411AlaLeu: 6.411 ± 0.079	1.725AlaMet: 1.725 ± 0.041	2.829AlaAsn: 2.829 ± 0.046	1.774AlaPro: 1.774 ± 0.034	2.023AlaGln: 2.023 ± 0.041	2.192AlaArg: 2.192 ± 0.041	3.88AlaSer: 3.88 ± 0.064	3.339AlaThr: 3.339 ± 0.05	4.721AlaVal: 4.721 ± 0.062	0.544AlaTrp: 0.544 ± 0.022	2.092AlaTyr: 2.092 ± 0.035	0.0AlaXaa: 0.0 ± 0.0
Cys	0.399CysAla: 0.399 ± 0.022	0.12CysCys: 0.12 ± 0.01	0.389CysAsp: 0.389 ± 0.018	0.502CysGlu: 0.502 ± 0.021	0.353CysPhe: 0.353 ± 0.017	0.631CysGly: 0.631 ± 0.023	0.195CysHis: 0.195 ± 0.013	0.566CysIle: 0.566 ± 0.021	0.425CysLys: 0.425 ± 0.019	0.73CysLeu: 0.73 ± 0.025	0.187CysMet: 0.187 ± 0.012	0.317CysAsn: 0.317 ± 0.017	0.337CysPro: 0.337 ± 0.016	0.252CysGln: 0.252 ± 0.014	0.274CysArg: 0.274 ± 0.015	0.553CysSer: 0.553 ± 0.022	0.373CysThr: 0.373 ± 0.017	0.423CysVal: 0.423 ± 0.017	0.068CysTrp: 0.068 ± 0.006	0.266CysTyr: 0.266 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	2.907AspAla: 2.907 ± 0.046	0.403AspCys: 0.403 ± 0.019	2.516AspAsp: 2.516 ± 0.054	4.551AspGlu: 4.551 ± 0.061	2.393AspPhe: 2.393 ± 0.04	3.152AspGly: 3.152 ± 0.059	1.207AspHis: 1.207 ± 0.03	4.169AspIle: 4.169 ± 0.065	3.252AspLys: 3.252 ± 0.046	5.154AspLeu: 5.154 ± 0.065	1.307AspMet: 1.307 ± 0.031	1.833AspAsn: 1.833 ± 0.037	1.988AspPro: 1.988 ± 0.039	2.191AspGln: 2.191 ± 0.042	2.121AspArg: 2.121 ± 0.037	2.797AspSer: 2.797 ± 0.048	2.422AspThr: 2.422 ± 0.041	3.733AspVal: 3.733 ± 0.048	0.592AspTrp: 0.592 ± 0.02	2.178AspTyr: 2.178 ± 0.045	0.0AspXaa: 0.0 ± 0.0
Glu	4.997GluAla: 4.997 ± 0.065	0.429GluCys: 0.429 ± 0.017	3.997GluAsp: 3.997 ± 0.059	7.42GluGlu: 7.42 ± 0.115	2.845GluPhe: 2.845 ± 0.046	4.414GluGly: 4.414 ± 0.062	1.577GluHis: 1.577 ± 0.037	5.943GluIle: 5.943 ± 0.073	7.018GluLys: 7.018 ± 0.088	7.397GluLeu: 7.397 ± 0.076	2.28GluMet: 2.28 ± 0.041	4.115GluAsn: 4.115 ± 0.06	1.818GluPro: 1.818 ± 0.041	3.507GluGln: 3.507 ± 0.068	3.297GluArg: 3.297 ± 0.058	3.766GluSer: 3.766 ± 0.055	3.829GluThr: 3.829 ± 0.054	5.227GluVal: 5.227 ± 0.068	0.781GluTrp: 0.781 ± 0.023	2.459GluTyr: 2.459 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	2.701PheAla: 2.701 ± 0.051	0.36PheCys: 0.36 ± 0.016	2.395PheAsp: 2.395 ± 0.039	2.915PheGlu: 2.915 ± 0.05	2.476PhePhe: 2.476 ± 0.058	3.101PheGly: 3.101 ± 0.051	1.072PheHis: 1.072 ± 0.029	4.233PheIle: 4.233 ± 0.078	2.604PheLys: 2.604 ± 0.045	4.798PheLeu: 4.798 ± 0.074	1.137PheMet: 1.137 ± 0.034	2.021PheAsn: 2.021 ± 0.039	1.625PhePro: 1.625 ± 0.037	1.733PheGln: 1.733 ± 0.038	1.478PheArg: 1.478 ± 0.031	3.361PheSer: 3.361 ± 0.051	2.614PheThr: 2.614 ± 0.04	3.119PheVal: 3.119 ± 0.05	0.456PheTrp: 0.456 ± 0.02	1.752PheTyr: 1.752 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	4.381GlyAla: 4.381 ± 0.071	0.603GlyCys: 0.603 ± 0.021	3.08GlyAsp: 3.08 ± 0.045	4.395GlyGlu: 4.395 ± 0.059	3.341GlyPhe: 3.341 ± 0.055	4.474GlyGly: 4.474 ± 0.072	1.297GlyHis: 1.297 ± 0.031	5.723GlyIle: 5.723 ± 0.076	4.873GlyLys: 4.873 ± 0.064	6.238GlyLeu: 6.238 ± 0.078	1.918GlyMet: 1.918 ± 0.041	2.766GlyAsn: 2.766 ± 0.058	1.583GlyPro: 1.583 ± 0.037	2.101GlyGln: 2.101 ± 0.038	2.393GlyArg: 2.393 ± 0.046	3.83GlySer: 3.83 ± 0.052	3.763GlyThr: 3.763 ± 0.063	4.842GlyVal: 4.842 ± 0.065	0.732GlyTrp: 0.732 ± 0.025	2.777GlyTyr: 2.777 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	1.223HisAla: 1.223 ± 0.029	0.19HisCys: 0.19 ± 0.012	1.082HisAsp: 1.082 ± 0.029	1.509HisGlu: 1.509 ± 0.031	1.074HisPhe: 1.074 ± 0.028	1.291HisGly: 1.291 ± 0.03	0.684HisHis: 0.684 ± 0.026	1.543HisIle: 1.543 ± 0.03	1.156HisLys: 1.156 ± 0.028	2.157HisLeu: 2.157 ± 0.041	0.486HisMet: 0.486 ± 0.019	0.851HisAsn: 0.851 ± 0.024	1.062HisPro: 1.062 ± 0.029	0.872HisGln: 0.872 ± 0.024	0.832HisArg: 0.832 ± 0.022	1.33HisSer: 1.33 ± 0.031	1.04HisThr: 1.04 ± 0.029	1.366HisVal: 1.366 ± 0.034	0.194HisTrp: 0.194 ± 0.012	0.979HisTyr: 0.979 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.391IleAla: 5.391 ± 0.069	0.664IleCys: 0.664 ± 0.025	4.502IleAsp: 4.502 ± 0.054	6.234IleGlu: 6.234 ± 0.076	3.464IlePhe: 3.464 ± 0.054	5.964IleGly: 5.964 ± 0.079	1.712IleHis: 1.712 ± 0.036	6.308IleIle: 6.308 ± 0.085	5.298IleLys: 5.298 ± 0.063	7.379IleLeu: 7.379 ± 0.089	1.861IleMet: 1.861 ± 0.033	3.8IleAsn: 3.8 ± 0.06	3.35IlePro: 3.35 ± 0.052	3.019IleGln: 3.019 ± 0.052	2.932IleArg: 2.932 ± 0.056	5.691IleSer: 5.691 ± 0.066	4.687IleThr: 4.687 ± 0.05	5.633IleVal: 5.633 ± 0.068	0.65IleTrp: 0.65 ± 0.023	2.647IleTyr: 2.647 ± 0.046	0.0IleXaa: 0.0 ± 0.0
Lys	4.374LysAla: 4.374 ± 0.063	0.353LysCys: 0.353 ± 0.016	4.103LysAsp: 4.103 ± 0.052	7.32LysGlu: 7.32 ± 0.073	2.078LysPhe: 2.078 ± 0.042	4.616LysGly: 4.616 ± 0.062	1.425LysHis: 1.425 ± 0.035	5.104LysIle: 5.104 ± 0.056	6.28LysLys: 6.28 ± 0.061	6.351LysLeu: 6.351 ± 0.076	2.216LysMet: 2.216 ± 0.04	3.828LysAsn: 3.828 ± 0.052	2.226LysPro: 2.226 ± 0.043	3.431LysGln: 3.431 ± 0.052	3.276LysArg: 3.276 ± 0.049	3.893LysSer: 3.893 ± 0.057	3.749LysThr: 3.749 ± 0.05	4.932LysVal: 4.932 ± 0.064	0.792LysTrp: 0.792 ± 0.025	2.364LysTyr: 2.364 ± 0.041	0.0LysXaa: 0.0 ± 0.0
Leu	6.584LeuAla: 6.584 ± 0.079	0.689LeuCys: 0.689 ± 0.024	4.817LeuAsp: 4.817 ± 0.059	6.786LeuGlu: 6.786 ± 0.084	4.986LeuPhe: 4.986 ± 0.077	6.093LeuGly: 6.093 ± 0.079	2.006LeuHis: 2.006 ± 0.04	7.509LeuIle: 7.509 ± 0.088	7.067LeuLys: 7.067 ± 0.076	10.25LeuLeu: 10.25 ± 0.111	2.387LeuMet: 2.387 ± 0.045	4.671LeuAsn: 4.671 ± 0.053	3.643LeuPro: 3.643 ± 0.049	3.696LeuGln: 3.696 ± 0.056	3.352LeuArg: 3.352 ± 0.056	7.32LeuSer: 7.32 ± 0.081	5.89LeuThr: 5.89 ± 0.069	6.248LeuVal: 6.248 ± 0.075	0.793LeuTrp: 0.793 ± 0.025	3.274LeuTyr: 3.274 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	1.728MetAla: 1.728 ± 0.033	0.149MetCys: 0.149 ± 0.011	1.303MetAsp: 1.303 ± 0.033	1.859MetGlu: 1.859 ± 0.038	1.137MetPhe: 1.137 ± 0.032	1.625MetGly: 1.625 ± 0.034	0.384MetHis: 0.384 ± 0.017	2.311MetIle: 2.311 ± 0.035	2.569MetLys: 2.569 ± 0.043	2.49MetLeu: 2.49 ± 0.04	0.865MetMet: 0.865 ± 0.024	1.671MetAsn: 1.671 ± 0.033	0.862MetPro: 0.862 ± 0.028	0.794MetGln: 0.794 ± 0.026	0.954MetArg: 0.954 ± 0.027	1.708MetSer: 1.708 ± 0.036	1.573MetThr: 1.573 ± 0.029	1.69MetVal: 1.69 ± 0.03	0.168MetTrp: 0.168 ± 0.01	0.743MetTyr: 0.743 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	2.576AsnAla: 2.576 ± 0.045	0.339AsnCys: 0.339 ± 0.016	2.439AsnAsp: 2.439 ± 0.043	4.157AsnGlu: 4.157 ± 0.059	1.714AsnPhe: 1.714 ± 0.039	3.419AsnGly: 3.419 ± 0.065	1.125AsnHis: 1.125 ± 0.026	3.747AsnIle: 3.747 ± 0.054	3.447AsnLys: 3.447 ± 0.058	4.124AsnLeu: 4.124 ± 0.054	1.309AsnMet: 1.309 ± 0.026	2.332AsnAsn: 2.332 ± 0.049	2.105AsnPro: 2.105 ± 0.042	2.161AsnGln: 2.161 ± 0.039	2.056AsnArg: 2.056 ± 0.038	2.871AsnSer: 2.871 ± 0.053	2.401AsnThr: 2.401 ± 0.045	3.216AsnVal: 3.216 ± 0.047	0.551AsnTrp: 0.551 ± 0.022	1.685AsnTyr: 1.685 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	1.967ProAla: 1.967 ± 0.04	0.229ProCys: 0.229 ± 0.012	1.881ProAsp: 1.881 ± 0.045	2.787ProGlu: 2.787 ± 0.044	1.879ProPhe: 1.879 ± 0.038	2.001ProGly: 2.001 ± 0.043	0.763ProHis: 0.763 ± 0.022	2.893ProIle: 2.893 ± 0.047	2.131ProLys: 2.131 ± 0.044	3.287ProLeu: 3.287 ± 0.051	0.732ProMet: 0.732 ± 0.021	1.738ProAsn: 1.738 ± 0.042	0.97ProPro: 0.97 ± 0.035	1.054ProGln: 1.054 ± 0.03	1.032ProArg: 1.032 ± 0.027	2.287ProSer: 2.287 ± 0.041	2.019ProThr: 2.019 ± 0.041	2.628ProVal: 2.628 ± 0.041	0.336ProTrp: 0.336 ± 0.017	1.411ProTyr: 1.411 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	2.596GlnAla: 2.596 ± 0.047	0.198GlnCys: 0.198 ± 0.014	1.769GlnAsp: 1.769 ± 0.036	2.986GlnGlu: 2.986 ± 0.05	1.671GlnPhe: 1.671 ± 0.031	2.038GlnGly: 2.038 ± 0.037	0.877GlnHis: 0.877 ± 0.025	2.738GlnIle: 2.738 ± 0.049	2.935GlnLys: 2.935 ± 0.053	4.264GlnLeu: 4.264 ± 0.058	1.069GlnMet: 1.069 ± 0.028	1.794GlnAsn: 1.794 ± 0.039	1.192GlnPro: 1.192 ± 0.034	2.024GlnGln: 2.024 ± 0.057	1.405GlnArg: 1.405 ± 0.032	2.395GlnSer: 2.395 ± 0.045	2.073GlnThr: 2.073 ± 0.036	2.422GlnVal: 2.422 ± 0.046	0.374GlnTrp: 0.374 ± 0.017	1.456GlnTyr: 1.456 ± 0.036	0.0GlnXaa: 0.0 ± 0.0
Arg	2.171ArgAla: 2.171 ± 0.041	0.259ArgCys: 0.259 ± 0.015	1.9ArgAsp: 1.9 ± 0.041	3.034ArgGlu: 3.034 ± 0.056	1.829ArgPhe: 1.829 ± 0.038	2.11ArgGly: 2.11 ± 0.037	0.72ArgHis: 0.72 ± 0.023	2.857ArgIle: 2.857 ± 0.05	3.204ArgLys: 3.204 ± 0.053	3.638ArgLeu: 3.638 ± 0.054	1.14ArgMet: 1.14 ± 0.027	1.952ArgAsn: 1.952 ± 0.042	1.149ArgPro: 1.149 ± 0.028	1.361ArgGln: 1.361 ± 0.037	1.674ArgArg: 1.674 ± 0.033	2.174ArgSer: 2.174 ± 0.042	1.967ArgThr: 1.967 ± 0.039	2.539ArgVal: 2.539 ± 0.045	0.374ArgTrp: 0.374 ± 0.015	1.508ArgTyr: 1.508 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	3.518SerAla: 3.518 ± 0.057	0.441SerCys: 0.441 ± 0.021	2.973SerAsp: 2.973 ± 0.053	4.306SerGlu: 4.306 ± 0.055	3.554SerPhe: 3.554 ± 0.052	4.204SerGly: 4.204 ± 0.061	1.305SerHis: 1.305 ± 0.032	5.786SerIle: 5.786 ± 0.066	4.283SerLys: 4.283 ± 0.059	6.687SerLeu: 6.687 ± 0.079	1.741SerMet: 1.741 ± 0.033	2.886SerAsn: 2.886 ± 0.046	2.081SerPro: 2.081 ± 0.041	2.226SerGln: 2.226 ± 0.04	2.218SerArg: 2.218 ± 0.042	4.547SerSer: 4.547 ± 0.064	3.6SerThr: 3.6 ± 0.055	4.307SerVal: 4.307 ± 0.058	0.633SerTrp: 0.633 ± 0.02	2.509SerTyr: 2.509 ± 0.039	0.0SerXaa: 0.0 ± 0.0
Thr	3.488ThrAla: 3.488 ± 0.055	0.418ThrCys: 0.418 ± 0.018	2.633ThrAsp: 2.633 ± 0.041	3.657ThrGlu: 3.657 ± 0.056	2.764ThrPhe: 2.764 ± 0.051	3.821ThrGly: 3.821 ± 0.056	1.037ThrHis: 1.037 ± 0.026	4.957ThrIle: 4.957 ± 0.066	3.707ThrLys: 3.707 ± 0.051	5.383ThrLeu: 5.383 ± 0.069	1.276ThrMet: 1.276 ± 0.034	2.841ThrAsn: 2.841 ± 0.046	2.137ThrPro: 2.137 ± 0.038	1.537ThrGln: 1.537 ± 0.035	1.743ThrArg: 1.743 ± 0.038	3.585ThrSer: 3.585 ± 0.06	3.07ThrThr: 3.07 ± 0.051	4.116ThrVal: 4.116 ± 0.057	0.55ThrTrp: 0.55 ± 0.022	2.131ThrTyr: 2.131 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	4.552ValAla: 4.552 ± 0.07	0.565ValCys: 0.565 ± 0.021	3.713ValAsp: 3.713 ± 0.053	5.131ValGlu: 5.131 ± 0.072	3.021ValPhe: 3.021 ± 0.047	4.528ValGly: 4.528 ± 0.069	1.31ValHis: 1.31 ± 0.037	5.709ValIle: 5.709 ± 0.062	4.909ValLys: 4.909 ± 0.055	6.543ValLeu: 6.543 ± 0.077	1.783ValMet: 1.783 ± 0.035	3.34ValAsn: 3.34 ± 0.05	2.496ValPro: 2.496 ± 0.046	2.328ValGln: 2.328 ± 0.043	2.414ValArg: 2.414 ± 0.048	4.802ValSer: 4.802 ± 0.066	4.109ValThr: 4.109 ± 0.054	5.046ValVal: 5.046 ± 0.072	0.602ValTrp: 0.602 ± 0.02	2.196ValTyr: 2.196 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	0.537TrpAla: 0.537 ± 0.021	0.088TrpCys: 0.088 ± 0.007	0.501TrpAsp: 0.501 ± 0.021	0.668TrpGlu: 0.668 ± 0.025	0.473TrpPhe: 0.473 ± 0.019	0.634TrpGly: 0.634 ± 0.021	0.195TrpHis: 0.195 ± 0.011	0.839TrpIle: 0.839 ± 0.026	0.726TrpLys: 0.726 ± 0.022	1.065TrpLeu: 1.065 ± 0.028	0.294TrpMet: 0.294 ± 0.016	0.56TrpAsn: 0.56 ± 0.019	0.211TrpPro: 0.211 ± 0.014	0.335TrpGln: 0.335 ± 0.017	0.363TrpArg: 0.363 ± 0.017	0.606TrpSer: 0.606 ± 0.021	0.491TrpThr: 0.491 ± 0.02	0.651TrpVal: 0.651 ± 0.024	0.13TrpTrp: 0.13 ± 0.011	0.344TrpTyr: 0.344 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.908TyrAla: 1.908 ± 0.037	0.324TyrCys: 0.324 ± 0.018	1.949TyrAsp: 1.949 ± 0.036	2.683TyrGlu: 2.683 ± 0.048	1.923TyrPhe: 1.923 ± 0.041	2.449TyrGly: 2.449 ± 0.052	0.885TyrHis: 0.885 ± 0.025	2.614TyrIle: 2.614 ± 0.045	2.335TyrLys: 2.335 ± 0.038	3.715TyrLeu: 3.715 ± 0.056	0.868TyrMet: 0.868 ± 0.021	1.628TyrAsn: 1.628 ± 0.036	1.416TyrPro: 1.416 ± 0.033	1.633TyrGln: 1.633 ± 0.03	1.556TyrArg: 1.556 ± 0.035	2.41TyrSer: 2.41 ± 0.04	1.846TyrThr: 1.846 ± 0.038	2.235TyrVal: 2.235 ± 0.031	0.394TyrTrp: 0.394 ± 0.016	1.531TyrTyr: 1.531 ± 0.038	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5011 proteins (1419903 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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