Colorado tick fever virus (strain USA/Florio N-7180) (CTFV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Coltivirus; Colorado tick fever coltivirus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9ENL0|VP6_CTFVL Uncharacterized protein VP6 OS=Colorado tick fever virus (strain USA/Florio N-7180) OX=648168 PE=4 SV=1
MM1 pKa = 7.75AARR4 pKa = 11.84LPIDD8 pKa = 3.7AFGMSVIQQNGLKK21 pKa = 10.15VYY23 pKa = 9.95TILPTSNLSNLSDD36 pKa = 3.76QISQNKK42 pKa = 9.35LYY44 pKa = 10.61AVSEE48 pKa = 4.15RR49 pKa = 11.84HH50 pKa = 5.93VRR52 pKa = 11.84EE53 pKa = 3.42LDD55 pKa = 3.27QRR57 pKa = 11.84DD58 pKa = 3.25KK59 pKa = 11.95GKK61 pKa = 10.51VKK63 pKa = 10.37KK64 pKa = 10.27IKK66 pKa = 10.62FIVTSKK72 pKa = 10.94SVDD75 pKa = 3.29YY76 pKa = 10.47EE77 pKa = 4.0NWFGPDD83 pKa = 3.02TDD85 pKa = 4.17TEE87 pKa = 4.29IIQYY91 pKa = 10.43LEE93 pKa = 3.89EE94 pKa = 5.81GGLYY98 pKa = 9.56HH99 pKa = 7.68ALYY102 pKa = 9.99NACKK106 pKa = 8.3EE107 pKa = 4.34QKK109 pKa = 8.84STPSFVASPSNPAPVSLKK127 pKa = 10.41QKK129 pKa = 10.16EE130 pKa = 4.18ILSNWKK136 pKa = 9.95LGTIVEE142 pKa = 4.96FCQAVGLNRR151 pKa = 11.84TPEE154 pKa = 4.12EE155 pKa = 3.87PWRR158 pKa = 11.84QLARR162 pKa = 11.84SYY164 pKa = 11.59GLIFISEE171 pKa = 4.18VGALASTHH179 pKa = 5.34VASLDD184 pKa = 3.21ADD186 pKa = 3.9LAKK189 pKa = 10.54FEE191 pKa = 4.18QRR193 pKa = 11.84EE194 pKa = 4.0QRR196 pKa = 11.84WMKK199 pKa = 9.57MVDD202 pKa = 3.48YY203 pKa = 10.44KK204 pKa = 11.35EE205 pKa = 4.81SFAHH209 pKa = 7.23LGTDD213 pKa = 4.01FNRR216 pKa = 11.84YY217 pKa = 8.52AFCPLIPPPCPEE229 pKa = 4.65DD230 pKa = 3.71SEE232 pKa = 4.4EE233 pKa = 4.85AILIHH238 pKa = 5.99GSWVRR243 pKa = 11.84SEE245 pKa = 4.05EE246 pKa = 3.94HH247 pKa = 7.19DD248 pKa = 3.48GGLFVLFKK256 pKa = 10.54RR257 pKa = 11.84VTIMDD262 pKa = 4.01RR263 pKa = 11.84HH264 pKa = 4.75QVMGARR270 pKa = 11.84APSEE274 pKa = 4.21DD275 pKa = 4.25EE276 pKa = 4.67EE277 pKa = 4.52EE278 pKa = 4.72SSDD281 pKa = 3.7PTEE284 pKa = 4.24GSMDD288 pKa = 3.54EE289 pKa = 4.35VTHH292 pKa = 4.65QTEE295 pKa = 4.34TLSLDD300 pKa = 4.1SIPPPDD306 pKa = 4.55FSRR309 pKa = 11.84APVIVTGMTSAKK321 pKa = 10.48ASTSYY326 pKa = 11.14SRR328 pKa = 11.84DD329 pKa = 3.47EE330 pKa = 4.3PPEE333 pKa = 4.51DD334 pKa = 3.28EE335 pKa = 4.65GNRR338 pKa = 11.84ASARR342 pKa = 11.84PKK344 pKa = 10.48SSPCDD349 pKa = 3.21TCTDD353 pKa = 4.03DD354 pKa = 5.89SSLLQSLLSTDD365 pKa = 2.8WVTSVSTPLHH375 pKa = 6.47GPTLPIDD382 pKa = 3.68AAKK385 pKa = 10.17DD386 pKa = 3.34LSVASEE392 pKa = 4.4SEE394 pKa = 4.62DD395 pKa = 3.54EE396 pKa = 4.43VSMSQSDD403 pKa = 4.51APPEE407 pKa = 3.83TAMDD411 pKa = 3.54VSANHH416 pKa = 6.83KK417 pKa = 10.24PNTDD421 pKa = 3.52SAHH424 pKa = 6.49CAPSPLPQRR433 pKa = 11.84ARR435 pKa = 11.84KK436 pKa = 9.03IRR438 pKa = 11.84PTEE441 pKa = 3.38ARR443 pKa = 11.84RR444 pKa = 11.84RR445 pKa = 11.84AEE447 pKa = 3.75RR448 pKa = 11.84EE449 pKa = 3.67KK450 pKa = 11.01RR451 pKa = 11.84KK452 pKa = 10.12LKK454 pKa = 10.11PYY456 pKa = 7.91YY457 pKa = 10.09RR458 pKa = 11.84SMEE461 pKa = 4.05EE462 pKa = 4.36CFVPACDD469 pKa = 3.22IEE471 pKa = 4.72SYY473 pKa = 8.39RR474 pKa = 11.84TSVIVQPLPQNVLPFPGTQLTRR496 pKa = 11.84EE497 pKa = 4.09TVLRR501 pKa = 11.84KK502 pKa = 9.92ALVRR506 pKa = 11.84EE507 pKa = 4.5CEE509 pKa = 3.93RR510 pKa = 11.84VAPSQAPDD518 pKa = 3.69PEE520 pKa = 4.37EE521 pKa = 5.24LLDD524 pKa = 3.95SDD526 pKa = 4.18TVKK529 pKa = 10.27ICEE532 pKa = 3.97GRR534 pKa = 11.84AKK536 pKa = 10.65VMYY539 pKa = 10.0EE540 pKa = 4.31SIPSHH545 pKa = 5.63LRR547 pKa = 11.84GVEE550 pKa = 3.93IEE552 pKa = 4.43SVAPEE557 pKa = 4.13SNEE560 pKa = 3.42VSVIYY565 pKa = 9.06PRR567 pKa = 11.84PQISMPDD574 pKa = 3.37NFSLGNEE581 pKa = 4.07EE582 pKa = 3.88EE583 pKa = 4.4RR584 pKa = 11.84IRR586 pKa = 11.84TYY588 pKa = 10.02VRR590 pKa = 11.84KK591 pKa = 8.22RR592 pKa = 11.84TIMEE596 pKa = 4.28RR597 pKa = 11.84EE598 pKa = 3.92SGGYY602 pKa = 10.56AMLRR606 pKa = 11.84PGYY609 pKa = 9.49GAEE612 pKa = 4.03ALEE615 pKa = 4.09HH616 pKa = 5.83ARR618 pKa = 11.84YY619 pKa = 9.9VSAAGVPIPEE629 pKa = 4.79LRR631 pKa = 11.84GQVLRR636 pKa = 11.84SRR638 pKa = 11.84ASRR641 pKa = 11.84EE642 pKa = 3.85MMSQTDD648 pKa = 3.79LRR650 pKa = 11.84LLEE653 pKa = 4.69LMMPVVNVPPEE664 pKa = 4.33GVDD667 pKa = 3.27KK668 pKa = 11.25DD669 pKa = 3.74LLAVYY674 pKa = 9.46KK675 pKa = 9.79ATVMDD680 pKa = 4.35ILTGG684 pKa = 3.64

Molecular weight: 76.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9ENK8|VP8_CTFVL Uncharacterized protein VP8 OS=Colorado tick fever virus (strain USA/Florio N-7180) OX=648168 PE=4 SV=1
MM1 pKa = 7.78LKK3 pKa = 10.3RR4 pKa = 11.84PAYY7 pKa = 10.39YY8 pKa = 10.52NIFLLPTGRR17 pKa = 11.84RR18 pKa = 11.84LAKK21 pKa = 9.72TYY23 pKa = 10.99NILEE27 pKa = 4.14NLITLPKK34 pKa = 9.5TDD36 pKa = 2.93WKK38 pKa = 10.87LYY40 pKa = 7.44EE41 pKa = 4.46TIEE44 pKa = 4.51QKK46 pKa = 10.68LKK48 pKa = 10.86WMIQEE53 pKa = 4.15YY54 pKa = 10.2RR55 pKa = 11.84SDD57 pKa = 2.93WRR59 pKa = 11.84DD60 pKa = 2.98RR61 pKa = 11.84LSHH64 pKa = 6.12HH65 pKa = 6.23EE66 pKa = 4.05RR67 pKa = 11.84YY68 pKa = 9.62GMKK71 pKa = 9.98VRR73 pKa = 11.84LTYY76 pKa = 10.32RR77 pKa = 11.84SEE79 pKa = 4.09SEE81 pKa = 3.91RR82 pKa = 11.84TKK84 pKa = 11.01LEE86 pKa = 3.84EE87 pKa = 5.08KK88 pKa = 9.6ITDD91 pKa = 3.66LVISHH96 pKa = 6.51GKK98 pKa = 8.98KK99 pKa = 9.74ASTVHH104 pKa = 5.51FCTRR108 pKa = 11.84EE109 pKa = 4.02SYY111 pKa = 10.23KK112 pKa = 10.84GRR114 pKa = 11.84VRR116 pKa = 11.84ANQILILLEE125 pKa = 4.0PEE127 pKa = 4.06GLGEE131 pKa = 4.43VEE133 pKa = 4.16GLTVRR138 pKa = 11.84AEE140 pKa = 4.36GNWMSLVTHH149 pKa = 6.75CLFILGSTLTCFGFVDD165 pKa = 4.42PARR168 pKa = 11.84GCRR171 pKa = 11.84FNLLPYY177 pKa = 10.38IKK179 pKa = 9.3TLHH182 pKa = 6.85PNDD185 pKa = 3.49KK186 pKa = 9.98WLLDD190 pKa = 3.97LQASWRR196 pKa = 11.84LEE198 pKa = 3.96YY199 pKa = 10.7GVSRR203 pKa = 11.84SIEE206 pKa = 3.83EE207 pKa = 3.82GALYY211 pKa = 10.62DD212 pKa = 5.02FFAEE216 pKa = 4.14SHH218 pKa = 5.24TLYY221 pKa = 10.78AVRR224 pKa = 11.84TWPGCEE230 pKa = 3.89KK231 pKa = 10.95YY232 pKa = 10.73LGALEE237 pKa = 4.71AFVGRR242 pKa = 11.84LAVLPAQVLTSKK254 pKa = 10.31EE255 pKa = 3.64HH256 pKa = 6.91DD257 pKa = 3.87FQSKK261 pKa = 10.11LMSKK265 pKa = 10.1AKK267 pKa = 9.04KK268 pKa = 8.68TGFHH272 pKa = 5.57YY273 pKa = 10.44LYY275 pKa = 9.9YY276 pKa = 10.58LVSFTMKK283 pKa = 10.14TSLTDD288 pKa = 4.3RR289 pKa = 11.84IVKK292 pKa = 9.69EE293 pKa = 3.68ALATVKK299 pKa = 10.22VFSFVVGRR307 pKa = 11.84DD308 pKa = 3.33HH309 pKa = 7.27LPAVGVYY316 pKa = 10.18SGEE319 pKa = 4.21DD320 pKa = 3.41FSKK323 pKa = 10.68QFLSMAVGTMDD334 pKa = 3.25TPSRR338 pKa = 11.84YY339 pKa = 10.11AITIVSGMQVDD350 pKa = 3.92VDD352 pKa = 4.16VKK354 pKa = 10.83SVTGIASFKK363 pKa = 10.88DD364 pKa = 3.94GTQHH368 pKa = 6.91TIVDD372 pKa = 4.82EE373 pKa = 4.64ILLQPARR380 pKa = 11.84LVLLGRR386 pKa = 11.84KK387 pKa = 9.36GGGKK391 pKa = 9.5SRR393 pKa = 11.84LSKK396 pKa = 10.41IFSEE400 pKa = 4.33LGYY403 pKa = 10.91NVLDD407 pKa = 3.51SDD409 pKa = 4.36TYY411 pKa = 11.39GKK413 pKa = 10.52VLTLVADD420 pKa = 4.33RR421 pKa = 11.84GEE423 pKa = 4.89DD424 pKa = 3.82GLDD427 pKa = 3.42DD428 pKa = 4.22ALKK431 pKa = 10.61KK432 pKa = 10.08FVRR435 pKa = 11.84LTPDD439 pKa = 2.54EE440 pKa = 4.52RR441 pKa = 11.84KK442 pKa = 9.55AVPSIFEE449 pKa = 4.1TEE451 pKa = 3.68MDD453 pKa = 3.79RR454 pKa = 11.84LCEE457 pKa = 4.13VFGSRR462 pKa = 11.84GLRR465 pKa = 11.84PYY467 pKa = 10.64AQRR470 pKa = 11.84CQQQAGRR477 pKa = 11.84LHH479 pKa = 6.28WEE481 pKa = 4.39LYY483 pKa = 10.42AAFQEE488 pKa = 5.12FYY490 pKa = 10.94DD491 pKa = 3.5RR492 pKa = 11.84TIRR495 pKa = 11.84VITPDD500 pKa = 3.25KK501 pKa = 10.9FRR503 pKa = 11.84FAYY506 pKa = 9.78FAEE509 pKa = 4.34LEE511 pKa = 4.21RR512 pKa = 11.84GGFDD516 pKa = 4.95DD517 pKa = 5.29NGLTFSPEE525 pKa = 3.57LKK527 pKa = 9.33TVVFVHH533 pKa = 6.31SMPEE537 pKa = 3.66LFEE540 pKa = 5.74AMGGCVAEE548 pKa = 4.2IVPTHH553 pKa = 5.11STRR556 pKa = 11.84LAILLRR562 pKa = 11.84GQGLSVNAEE571 pKa = 3.89LHH573 pKa = 6.08LHH575 pKa = 6.41DD576 pKa = 6.11FYY578 pKa = 12.03VALNQNGARR587 pKa = 11.84KK588 pKa = 9.73VSLGWLVHH596 pKa = 6.38ALNEE600 pKa = 4.11LLKK603 pKa = 10.22MRR605 pKa = 5.02

Molecular weight: 68.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

9623

185

1435

740.2

83.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.596 ± 0.317

1.59 ± 0.141

5.528 ± 0.15

6.318 ± 0.253

4.417 ± 0.265

7.139 ± 0.394

2.39 ± 0.149

4.666 ± 0.285

3.938 ± 0.297

9.664 ± 0.338

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.92 ± 0.221

3.315 ± 0.159

4.728 ± 0.389

3.648 ± 0.223

7.212 ± 0.331

6.453 ± 0.425

5.414 ± 0.275

8.074 ± 0.281

1.611 ± 0.156

3.367 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski