Nitratireductor basaltis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Nitratireductor

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3449 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A084UBW2|A0A084UBW2_9RHIZ Uncharacterized protein OS=Nitratireductor basaltis OX=472175 GN=EL18_01483 PE=3 SV=1
MM1 pKa = 7.83KK2 pKa = 10.24YY3 pKa = 10.99LLMTAASAVFLAGSAFAQTSPDD25 pKa = 3.25TDD27 pKa = 3.7TNDD30 pKa = 3.6NNMTDD35 pKa = 3.88MMPGIDD41 pKa = 4.32VSNAMIDD48 pKa = 3.69GNKK51 pKa = 7.68ITGVTVTSNQPAYY64 pKa = 10.84VVIHH68 pKa = 6.31NEE70 pKa = 4.29GEE72 pKa = 4.33GAPPASLGHH81 pKa = 5.68MRR83 pKa = 11.84VEE85 pKa = 4.35PGEE88 pKa = 4.08TSDD91 pKa = 5.79LSIEE95 pKa = 4.4ADD97 pKa = 3.7GEE99 pKa = 4.23LDD101 pKa = 3.55PASGITLMLHH111 pKa = 6.6YY112 pKa = 7.52EE113 pKa = 4.28TNDD116 pKa = 2.95NNTYY120 pKa = 11.11DD121 pKa = 4.37FGPGSTDD128 pKa = 2.33VDD130 pKa = 4.41TPAMGADD137 pKa = 3.81GPINVPLQQ145 pKa = 3.41

Molecular weight: 15.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A084U6A4|A0A084U6A4_9RHIZ Alkylated DNA repair protein OS=Nitratireductor basaltis OX=472175 GN=EL18_02741 PE=4 SV=1
MM1 pKa = 7.79RR2 pKa = 11.84SLRR5 pKa = 11.84GLFVAALAVFAASAANASPFIGNLAAGAVQQAVQQVLSVPVRR47 pKa = 11.84RR48 pKa = 11.84PKK50 pKa = 10.83LQMPSLTTAQMRR62 pKa = 11.84VEE64 pKa = 4.16AQCLSLGIYY73 pKa = 10.12HH74 pKa = 6.76EE75 pKa = 4.68ARR77 pKa = 11.84GEE79 pKa = 4.24SIEE82 pKa = 4.08GQAAVAQVILNRR94 pKa = 11.84ARR96 pKa = 11.84SKK98 pKa = 10.66SYY100 pKa = 9.73PSSICGVIYY109 pKa = 10.72QNAYY113 pKa = 9.29KK114 pKa = 10.76LNRR117 pKa = 11.84CQFSFTCDD125 pKa = 2.83RR126 pKa = 11.84VSDD129 pKa = 4.41FPRR132 pKa = 11.84DD133 pKa = 3.31EE134 pKa = 4.38KK135 pKa = 10.74SWRR138 pKa = 11.84RR139 pKa = 11.84AIKK142 pKa = 10.37LSEE145 pKa = 4.28ALLCANCSHH154 pKa = 7.49AEE156 pKa = 3.88RR157 pKa = 11.84SVLKK161 pKa = 10.71SIAPFHH167 pKa = 6.78RR168 pKa = 11.84ATHH171 pKa = 4.76YY172 pKa = 9.58HH173 pKa = 5.17ATYY176 pKa = 10.09VRR178 pKa = 11.84PVWARR183 pKa = 11.84KK184 pKa = 8.97LRR186 pKa = 11.84LVGRR190 pKa = 11.84IGTHH194 pKa = 5.73IFFEE198 pKa = 4.44SDD200 pKa = 2.7RR201 pKa = 11.84VLRR204 pKa = 11.84RR205 pKa = 11.84MM206 pKa = 4.07

Molecular weight: 22.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3449

0

3449

1074602

30

2861

311.6

33.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.774 ± 0.06

0.796 ± 0.01

5.709 ± 0.043

6.695 ± 0.046

3.867 ± 0.03

8.429 ± 0.039

2.042 ± 0.019

5.459 ± 0.032

3.601 ± 0.03

9.914 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.713 ± 0.023

2.838 ± 0.028

4.779 ± 0.03

3.2 ± 0.025

6.765 ± 0.044

5.592 ± 0.028

5.079 ± 0.032

7.272 ± 0.031

1.232 ± 0.015

2.245 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski