Kytococcus sp. HMSC28H12

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Kytococcaceae; Kytococcus; unclassified Kytococcus

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1956 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1F1K7L6|A0A1F1K7L6_9MICO Polyisoprenoid-binding protein OS=Kytococcus sp. HMSC28H12 OX=1581067 GN=HMPREF3099_07985 PE=4 SV=1
MM1 pKa = 8.47DD2 pKa = 4.23YY3 pKa = 10.75PQDD6 pKa = 3.59LLYY9 pKa = 10.41TDD11 pKa = 2.83KK12 pKa = 11.52HH13 pKa = 4.75EE14 pKa = 4.39WLRR17 pKa = 11.84EE18 pKa = 4.09AGDD21 pKa = 3.56GVYY24 pKa = 9.95RR25 pKa = 11.84VGITDD30 pKa = 3.62YY31 pKa = 11.47AQDD34 pKa = 3.5ALGDD38 pKa = 3.99VVFVTLPAVGEE49 pKa = 4.3TFSRR53 pKa = 11.84DD54 pKa = 3.21DD55 pKa = 3.28AAGEE59 pKa = 4.24VEE61 pKa = 4.44STKK64 pKa = 10.72SVSDD68 pKa = 3.72VYY70 pKa = 11.46SPLSGEE76 pKa = 3.75ITAVNDD82 pKa = 3.91ALDD85 pKa = 3.92ATPEE89 pKa = 4.16NVNSDD94 pKa = 3.77PYY96 pKa = 10.07GEE98 pKa = 4.19GWIFEE103 pKa = 4.4IKK105 pKa = 10.32VSEE108 pKa = 4.18SSEE111 pKa = 4.41VEE113 pKa = 4.03DD114 pKa = 5.23LLDD117 pKa = 4.14AEE119 pKa = 5.63AYY121 pKa = 7.94TATLDD126 pKa = 3.4

Molecular weight: 13.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1F1JYC4|A0A1F1JYC4_9MICO Serine/threonine protein kinase OS=Kytococcus sp. HMSC28H12 OX=1581067 GN=HMPREF3099_09635 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84ARR15 pKa = 11.84KK16 pKa = 8.24HH17 pKa = 4.6GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.36GRR40 pKa = 11.84AKK42 pKa = 10.3ISAA45 pKa = 3.71

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1956

0

1956

613955

37

1629

313.9

33.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.287 ± 0.067

0.707 ± 0.014

6.063 ± 0.048

6.347 ± 0.061

2.598 ± 0.035

9.568 ± 0.046

2.39 ± 0.026

2.806 ± 0.04

1.914 ± 0.046

10.117 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.075 ± 0.025

1.625 ± 0.029

5.631 ± 0.044

3.03 ± 0.031

7.575 ± 0.057

4.9 ± 0.042

6.252 ± 0.036

9.788 ± 0.055

1.629 ± 0.029

1.698 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski