Amino acid dipepetide frequency for Kytococcus sp. HMSC28H12

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.381AlaAla: 19.381 ± 0.242
0.977AlaCys: 0.977 ± 0.045
8.014AlaAsp: 8.014 ± 0.131
9.046AlaGlu: 9.046 ± 0.161
3.306AlaPhe: 3.306 ± 0.082
13.271AlaGly: 13.271 ± 0.174
2.842AlaHis: 2.842 ± 0.076
3.22AlaIle: 3.22 ± 0.076
2.367AlaLys: 2.367 ± 0.079
14.126AlaLeu: 14.126 ± 0.203
3.29AlaMet: 3.29 ± 0.067
1.746AlaAsn: 1.746 ± 0.053
6.647AlaPro: 6.647 ± 0.137
4.098AlaGln: 4.098 ± 0.087
9.655AlaArg: 9.655 ± 0.153
6.103AlaSer: 6.103 ± 0.115
7.807AlaThr: 7.807 ± 0.125
12.595AlaVal: 12.595 ± 0.155
2.293AlaTrp: 2.293 ± 0.073
2.088AlaTyr: 2.088 ± 0.065
0.0AlaXaa: 0.0 ± 0.0
Cys
0.821CysAla: 0.821 ± 0.034
0.077CysCys: 0.077 ± 0.012
0.471CysAsp: 0.471 ± 0.03
0.391CysGlu: 0.391 ± 0.027
0.199CysPhe: 0.199 ± 0.021
0.857CysGly: 0.857 ± 0.042
0.192CysHis: 0.192 ± 0.019
0.192CysIle: 0.192 ± 0.017
0.114CysLys: 0.114 ± 0.014
0.635CysLeu: 0.635 ± 0.032
0.14CysMet: 0.14 ± 0.015
0.143CysAsn: 0.143 ± 0.015
0.437CysPro: 0.437 ± 0.029
0.181CysGln: 0.181 ± 0.018
0.48CysArg: 0.48 ± 0.031
0.417CysSer: 0.417 ± 0.027
0.513CysThr: 0.513 ± 0.039
0.601CysVal: 0.601 ± 0.029
0.107CysTrp: 0.107 ± 0.014
0.098CysTyr: 0.098 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
8.693AspAla: 8.693 ± 0.134
0.347AspCys: 0.347 ± 0.023
3.785AspAsp: 3.785 ± 0.1
4.513AspGlu: 4.513 ± 0.103
1.552AspPhe: 1.552 ± 0.054
5.803AspGly: 5.803 ± 0.121
1.699AspHis: 1.699 ± 0.064
1.521AspIle: 1.521 ± 0.064
0.972AspLys: 0.972 ± 0.035
6.794AspLeu: 6.794 ± 0.115
0.958AspMet: 0.958 ± 0.037
0.769AspAsn: 0.769 ± 0.041
4.502AspPro: 4.502 ± 0.097
1.629AspGln: 1.629 ± 0.05
4.619AspArg: 4.619 ± 0.089
2.241AspSer: 2.241 ± 0.062
2.994AspThr: 2.994 ± 0.069
5.355AspVal: 5.355 ± 0.087
0.894AspTrp: 0.894 ± 0.034
0.985AspTyr: 0.985 ± 0.045
0.0AspXaa: 0.0 ± 0.0
Glu
8.725GluAla: 8.725 ± 0.156
0.394GluCys: 0.394 ± 0.023
3.515GluAsp: 3.515 ± 0.083
4.005GluGlu: 4.005 ± 0.091
1.494GluPhe: 1.494 ± 0.054
5.684GluGly: 5.684 ± 0.11
1.907GluHis: 1.907 ± 0.061
1.885GluIle: 1.885 ± 0.067
1.661GluLys: 1.661 ± 0.065
6.557GluLeu: 6.557 ± 0.117
1.2GluMet: 1.2 ± 0.046
1.16GluAsn: 1.16 ± 0.05
3.249GluPro: 3.249 ± 0.084
2.666GluGln: 2.666 ± 0.065
5.51GluArg: 5.51 ± 0.108
2.476GluSer: 2.476 ± 0.07
2.761GluThr: 2.761 ± 0.072
6.673GluVal: 6.673 ± 0.118
0.816GluTrp: 0.816 ± 0.035
1.129GluTyr: 1.129 ± 0.043
0.0GluXaa: 0.0 ± 0.0
Phe
3.29PheAla: 3.29 ± 0.085
0.205PheCys: 0.205 ± 0.017
1.883PheAsp: 1.883 ± 0.057
1.508PheGlu: 1.508 ± 0.056
0.808PhePhe: 0.808 ± 0.044
2.859PheGly: 2.859 ± 0.071
0.669PheHis: 0.669 ± 0.034
0.689PheIle: 0.689 ± 0.036
0.414PheLys: 0.414 ± 0.027
2.432PheLeu: 2.432 ± 0.065
0.443PheMet: 0.443 ± 0.03
0.497PheAsn: 0.497 ± 0.032
1.197PhePro: 1.197 ± 0.047
0.637PheGln: 0.637 ± 0.036
1.531PheArg: 1.531 ± 0.051
1.49PheSer: 1.49 ± 0.047
1.992PheThr: 1.992 ± 0.058
2.487PheVal: 2.487 ± 0.058
0.454PheTrp: 0.454 ± 0.03
0.498PheTyr: 0.498 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
11.346GlyAla: 11.346 ± 0.153
0.801GlyCys: 0.801 ± 0.039
5.546GlyAsp: 5.546 ± 0.106
6.07GlyGlu: 6.07 ± 0.108
2.689GlyPhe: 2.689 ± 0.08
8.602GlyGly: 8.602 ± 0.153
2.363GlyHis: 2.363 ± 0.066
3.552GlyIle: 3.552 ± 0.079
2.166GlyLys: 2.166 ± 0.066
9.869GlyLeu: 9.869 ± 0.159
2.293GlyMet: 2.293 ± 0.056
1.534GlyAsn: 1.534 ± 0.047
4.647GlyPro: 4.647 ± 0.104
3.072GlyGln: 3.072 ± 0.069
7.52GlyArg: 7.52 ± 0.128
5.136GlySer: 5.136 ± 0.102
6.056GlyThr: 6.056 ± 0.101
8.628GlyVal: 8.628 ± 0.129
1.814GlyTrp: 1.814 ± 0.06
1.977GlyTyr: 1.977 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
2.754HisAla: 2.754 ± 0.067
0.181HisCys: 0.181 ± 0.017
1.45HisAsp: 1.45 ± 0.056
1.505HisGlu: 1.505 ± 0.052
0.546HisPhe: 0.546 ± 0.033
2.409HisGly: 2.409 ± 0.063
0.805HisHis: 0.805 ± 0.041
0.515HisIle: 0.515 ± 0.031
0.34HisLys: 0.34 ± 0.024
2.762HisLeu: 2.762 ± 0.08
0.34HisMet: 0.34 ± 0.023
0.362HisAsn: 0.362 ± 0.024
1.88HisPro: 1.88 ± 0.058
0.634HisGln: 0.634 ± 0.032
2.135HisArg: 2.135 ± 0.063
0.979HisSer: 0.979 ± 0.038
1.409HisThr: 1.409 ± 0.041
2.142HisVal: 2.142 ± 0.065
0.383HisTrp: 0.383 ± 0.026
0.37HisTyr: 0.37 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
4.005IleAla: 4.005 ± 0.1
0.241IleCys: 0.241 ± 0.018
2.14IleAsp: 2.14 ± 0.066
2.065IleGlu: 2.065 ± 0.071
0.674IlePhe: 0.674 ± 0.037
3.303IleGly: 3.303 ± 0.088
0.562IleHis: 0.562 ± 0.032
0.891IleIle: 0.891 ± 0.044
0.601IleLys: 0.601 ± 0.037
2.026IleLeu: 2.026 ± 0.078
0.463IleMet: 0.463 ± 0.026
0.598IleAsn: 0.598 ± 0.032
1.422IlePro: 1.422 ± 0.056
0.637IleGln: 0.637 ± 0.032
1.762IleArg: 1.762 ± 0.053
1.389IleSer: 1.389 ± 0.052
2.036IleThr: 2.036 ± 0.055
2.525IleVal: 2.525 ± 0.076
0.305IleTrp: 0.305 ± 0.02
0.419IleTyr: 0.419 ± 0.027
0.0IleXaa: 0.0 ± 0.0
Lys
2.622LysAla: 2.622 ± 0.088
0.09LysCys: 0.09 ± 0.013
1.311LysAsp: 1.311 ± 0.057
1.166LysGlu: 1.166 ± 0.056
0.393LysPhe: 0.393 ± 0.027
1.896LysGly: 1.896 ± 0.073
0.433LysHis: 0.433 ± 0.027
0.704LysIle: 0.704 ± 0.039
0.754LysLys: 0.754 ± 0.052
1.375LysLeu: 1.375 ± 0.056
0.383LysMet: 0.383 ± 0.024
0.48LysAsn: 0.48 ± 0.027
1.011LysPro: 1.011 ± 0.044
0.678LysGln: 0.678 ± 0.043
1.27LysArg: 1.27 ± 0.051
0.941LysSer: 0.941 ± 0.041
1.018LysThr: 1.018 ± 0.046
2.02LysVal: 2.02 ± 0.06
0.23LysTrp: 0.23 ± 0.021
0.368LysTyr: 0.368 ± 0.025
0.0LysXaa: 0.0 ± 0.0
Leu
14.706LeuAla: 14.706 ± 0.198
0.726LeuCys: 0.726 ± 0.036
6.207LeuAsp: 6.207 ± 0.107
5.898LeuGlu: 5.898 ± 0.122
2.362LeuPhe: 2.362 ± 0.072
9.888LeuGly: 9.888 ± 0.168
2.189LeuHis: 2.189 ± 0.062
2.13LeuIle: 2.13 ± 0.07
1.658LeuLys: 1.658 ± 0.06
10.665LeuLeu: 10.665 ± 0.181
1.774LeuMet: 1.774 ± 0.059
1.536LeuAsn: 1.536 ± 0.052
5.927LeuPro: 5.927 ± 0.108
2.653LeuGln: 2.653 ± 0.077
7.641LeuArg: 7.641 ± 0.127
5.044LeuSer: 5.044 ± 0.087
6.5LeuThr: 6.5 ± 0.119
10.776LeuVal: 10.776 ± 0.164
1.476LeuTrp: 1.476 ± 0.053
1.409LeuTyr: 1.409 ± 0.048
0.0LeuXaa: 0.0 ± 0.0
Met
2.867MetAla: 2.867 ± 0.072
0.132MetCys: 0.132 ± 0.016
1.093MetAsp: 1.093 ± 0.038
1.06MetGlu: 1.06 ± 0.049
0.495MetPhe: 0.495 ± 0.03
1.881MetGly: 1.881 ± 0.062
0.386MetHis: 0.386 ± 0.027
0.49MetIle: 0.49 ± 0.031
0.433MetLys: 0.433 ± 0.025
2.062MetLeu: 2.062 ± 0.051
0.404MetMet: 0.404 ± 0.029
0.448MetAsn: 0.448 ± 0.025
1.253MetPro: 1.253 ± 0.042
0.632MetGln: 0.632 ± 0.031
1.505MetArg: 1.505 ± 0.05
1.323MetSer: 1.323 ± 0.051
1.705MetThr: 1.705 ± 0.053
2.052MetVal: 2.052 ± 0.057
0.213MetTrp: 0.213 ± 0.019
0.314MetTyr: 0.314 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
2.047AsnAla: 2.047 ± 0.064
0.143AsnCys: 0.143 ± 0.017
0.873AsnAsp: 0.873 ± 0.046
0.766AsnGlu: 0.766 ± 0.032
0.445AsnPhe: 0.445 ± 0.031
1.51AsnGly: 1.51 ± 0.051
0.376AsnHis: 0.376 ± 0.026
0.58AsnIle: 0.58 ± 0.032
0.365AsnLys: 0.365 ± 0.03
1.694AsnLeu: 1.694 ± 0.056
0.303AsnMet: 0.303 ± 0.021
0.375AsnAsn: 0.375 ± 0.027
1.399AsnPro: 1.399 ± 0.05
0.477AsnGln: 0.477 ± 0.032
1.111AsnArg: 1.111 ± 0.039
0.761AsnSer: 0.761 ± 0.034
0.995AsnThr: 0.995 ± 0.039
1.44AsnVal: 1.44 ± 0.051
0.231AsnTrp: 0.231 ± 0.02
0.358AsnTyr: 0.358 ± 0.023
0.0AsnXaa: 0.0 ± 0.0
Pro
7.652ProAla: 7.652 ± 0.131
0.279ProCys: 0.279 ± 0.021
3.829ProAsp: 3.829 ± 0.083
4.515ProGlu: 4.515 ± 0.093
1.459ProPhe: 1.459 ± 0.048
5.965ProGly: 5.965 ± 0.098
1.391ProHis: 1.391 ± 0.049
1.169ProIle: 1.169 ± 0.045
0.928ProLys: 0.928 ± 0.04
4.727ProLeu: 4.727 ± 0.098
1.169ProMet: 1.169 ± 0.041
0.77ProAsn: 0.77 ± 0.04
2.334ProPro: 2.334 ± 0.074
1.901ProGln: 1.901 ± 0.051
3.665ProArg: 3.665 ± 0.09
3.062ProSer: 3.062 ± 0.084
3.798ProThr: 3.798 ± 0.099
5.657ProVal: 5.657 ± 0.09
1.111ProTrp: 1.111 ± 0.051
0.925ProTyr: 0.925 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
3.912GlnAla: 3.912 ± 0.076
0.165GlnCys: 0.165 ± 0.017
1.575GlnAsp: 1.575 ± 0.053
1.878GlnGlu: 1.878 ± 0.057
0.757GlnPhe: 0.757 ± 0.038
2.549GlnGly: 2.549 ± 0.073
0.783GlnHis: 0.783 ± 0.034
0.886GlnIle: 0.886 ± 0.043
0.671GlnLys: 0.671 ± 0.037
2.958GlnLeu: 2.958 ± 0.077
0.661GlnMet: 0.661 ± 0.031
0.503GlnAsn: 0.503 ± 0.028
1.784GlnPro: 1.784 ± 0.057
1.324GlnGln: 1.324 ± 0.051
2.772GlnArg: 2.772 ± 0.075
1.194GlnSer: 1.194 ± 0.043
1.384GlnThr: 1.384 ± 0.049
3.481GlnVal: 3.481 ± 0.079
0.511GlnTrp: 0.511 ± 0.03
0.551GlnTyr: 0.551 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
9.317ArgAla: 9.317 ± 0.126
0.521ArgCys: 0.521 ± 0.033
4.126ArgAsp: 4.126 ± 0.091
5.061ArgGlu: 5.061 ± 0.1
1.994ArgPhe: 1.994 ± 0.058
5.917ArgGly: 5.917 ± 0.104
1.946ArgHis: 1.946 ± 0.06
2.689ArgIle: 2.689 ± 0.073
1.404ArgLys: 1.404 ± 0.051
7.538ArgLeu: 7.538 ± 0.119
2.021ArgMet: 2.021 ± 0.051
1.225ArgAsn: 1.225 ± 0.049
4.103ArgPro: 4.103 ± 0.083
2.347ArgGln: 2.347 ± 0.064
7.321ArgArg: 7.321 ± 0.142
3.842ArgSer: 3.842 ± 0.084
5.101ArgThr: 5.101 ± 0.089
6.364ArgVal: 6.364 ± 0.119
1.497ArgTrp: 1.497 ± 0.05
1.42ArgTyr: 1.42 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
6.0SerAla: 6.0 ± 0.098
0.34SerCys: 0.34 ± 0.027
2.526SerAsp: 2.526 ± 0.067
2.534SerGlu: 2.534 ± 0.065
1.455SerPhe: 1.455 ± 0.05
5.518SerGly: 5.518 ± 0.101
1.003SerHis: 1.003 ± 0.042
1.446SerIle: 1.446 ± 0.061
0.899SerLys: 0.899 ± 0.048
4.593SerLeu: 4.593 ± 0.085
1.236SerMet: 1.236 ± 0.045
0.798SerAsn: 0.798 ± 0.038
3.06SerPro: 3.06 ± 0.088
1.402SerGln: 1.402 ± 0.049
3.482SerArg: 3.482 ± 0.077
3.036SerSer: 3.036 ± 0.091
3.66SerThr: 3.66 ± 0.078
4.259SerVal: 4.259 ± 0.088
0.855SerTrp: 0.855 ± 0.041
0.893SerTyr: 0.893 ± 0.039
0.0SerXaa: 0.0 ± 0.0
Thr
7.861ThrAla: 7.861 ± 0.129
0.472ThrCys: 0.472 ± 0.03
3.973ThrAsp: 3.973 ± 0.091
3.448ThrGlu: 3.448 ± 0.07
1.74ThrPhe: 1.74 ± 0.06
6.528ThrGly: 6.528 ± 0.106
1.394ThrHis: 1.394 ± 0.045
1.842ThrIle: 1.842 ± 0.058
1.082ThrLys: 1.082 ± 0.052
5.97ThrLeu: 5.97 ± 0.097
1.158ThrMet: 1.158 ± 0.045
1.034ThrAsn: 1.034 ± 0.043
4.271ThrPro: 4.271 ± 0.091
1.702ThrGln: 1.702 ± 0.05
3.992ThrArg: 3.992 ± 0.068
3.194ThrSer: 3.194 ± 0.074
4.377ThrThr: 4.377 ± 0.103
6.137ThrVal: 6.137 ± 0.108
1.093ThrTrp: 1.093 ± 0.048
1.257ThrTyr: 1.257 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
12.885ValAla: 12.885 ± 0.153
0.668ValCys: 0.668 ± 0.036
6.382ValAsp: 6.382 ± 0.106
6.155ValGlu: 6.155 ± 0.128
2.481ValPhe: 2.481 ± 0.076
8.12ValGly: 8.12 ± 0.135
2.321ValHis: 2.321 ± 0.067
2.842ValIle: 2.842 ± 0.079
1.679ValLys: 1.679 ± 0.061
10.665ValLeu: 10.665 ± 0.168
1.86ValMet: 1.86 ± 0.062
1.64ValAsn: 1.64 ± 0.053
5.486ValPro: 5.486 ± 0.095
2.616ValGln: 2.616 ± 0.068
7.126ValArg: 7.126 ± 0.118
4.565ValSer: 4.565 ± 0.089
6.377ValThr: 6.377 ± 0.103
11.475ValVal: 11.475 ± 0.185
1.23ValTrp: 1.23 ± 0.051
1.308ValTyr: 1.308 ± 0.044
0.0ValXaa: 0.0 ± 0.0
Trp
1.964TrpAla: 1.964 ± 0.063
0.163TrpCys: 0.163 ± 0.016
0.798TrpAsp: 0.798 ± 0.039
0.836TrpGlu: 0.836 ± 0.04
0.595TrpPhe: 0.595 ± 0.032
1.37TrpGly: 1.37 ± 0.047
0.329TrpHis: 0.329 ± 0.024
0.423TrpIle: 0.423 ± 0.028
0.261TrpLys: 0.261 ± 0.02
1.788TrpLeu: 1.788 ± 0.064
0.425TrpMet: 0.425 ± 0.023
0.334TrpAsn: 0.334 ± 0.023
0.811TrpPro: 0.811 ± 0.039
0.578TrpGln: 0.578 ± 0.028
1.295TrpArg: 1.295 ± 0.048
0.914TrpSer: 0.914 ± 0.044
0.981TrpThr: 0.981 ± 0.038
1.611TrpVal: 1.611 ± 0.061
0.544TrpTrp: 0.544 ± 0.034
0.267TrpTyr: 0.267 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.025TyrAla: 2.025 ± 0.056
0.143TyrCys: 0.143 ± 0.015
1.13TyrAsp: 1.13 ± 0.041
1.047TyrGlu: 1.047 ± 0.048
0.542TyrPhe: 0.542 ± 0.031
1.753TyrGly: 1.753 ± 0.055
0.306TyrHis: 0.306 ± 0.025
0.396TyrIle: 0.396 ± 0.027
0.373TyrLys: 0.373 ± 0.028
1.93TyrLeu: 1.93 ± 0.061
0.225TyrMet: 0.225 ± 0.021
0.296TyrAsn: 0.296 ± 0.023
0.888TyrPro: 0.888 ± 0.04
0.437TyrGln: 0.437 ± 0.03
1.36TyrArg: 1.36 ± 0.049
0.891TyrSer: 0.891 ± 0.045
1.06TyrThr: 1.06 ± 0.043
1.603TyrVal: 1.603 ± 0.057
0.228TyrTrp: 0.228 ± 0.019
0.34TyrTyr: 0.34 ± 0.029
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1956 proteins (613955 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski