Marinobacter mobilis
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3442 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H3DCN1|A0A1H3DCN1_9ALTE DNA-binding transcriptional response regulator NtrC family contains REC AAA-type ATPase and a Fis-type DNA-binding domains OS=Marinobacter mobilis OX=488533 GN=SAMN04487960_11226 PE=4 SV=1
MM1 pKa = 7.09 QNSAALWTLPLALTLAACNVDD22 pKa = 2.82 ISTTEE27 pKa = 3.88 VTPGQPDD34 pKa = 3.81 SEE36 pKa = 4.76 TPTPVKK42 pKa = 10.71 DD43 pKa = 3.67 LDD45 pKa = 3.86 SDD47 pKa = 4.32 GVADD51 pKa = 4.77 SADD54 pKa = 3.44 NCLLVPNADD63 pKa = 3.47 QANSNGDD70 pKa = 3.87 ALGDD74 pKa = 3.71 ACDD77 pKa = 3.47 IDD79 pKa = 4.2 TPVVEE84 pKa = 5.1 GKK86 pKa = 9.91 YY87 pKa = 10.19 QLTIYY92 pKa = 10.38 HH93 pKa = 6.62 EE94 pKa = 4.74 LGSEE98 pKa = 4.16 YY99 pKa = 10.78 LDD101 pKa = 3.87 AASGVCVSATDD112 pKa = 3.49 SPFTVDD118 pKa = 3.53 VLVKK122 pKa = 10.24 GSQVFLSEE130 pKa = 4.06 EE131 pKa = 3.83 GGEE134 pKa = 3.9 RR135 pKa = 11.84 RR136 pKa = 11.84 FYY138 pKa = 11.26 GIADD142 pKa = 3.4 TSGNLEE148 pKa = 4.34 VFTHH152 pKa = 6.89 PEE154 pKa = 3.79 GQFAATNGYY163 pKa = 9.43 FSEE166 pKa = 4.44 GTRR169 pKa = 11.84 RR170 pKa = 11.84 ITFDD174 pKa = 3.98 YY175 pKa = 10.97 SDD177 pKa = 3.51 QQSNLDD183 pKa = 3.61 GTVSCNIAARR193 pKa = 11.84 VEE195 pKa = 4.55 ANFNYY200 pKa = 8.24 ATSLIAPAAMVPPGLSPGDD219 pKa = 3.22 TFYY222 pKa = 10.94 IVFVTSAGTVANLSVDD238 pKa = 3.38 AFNLFVNDD246 pKa = 3.53 VADD249 pKa = 4.42 GSSLKK254 pKa = 10.14 GTDD257 pKa = 3.55 NGDD260 pKa = 4.01 LRR262 pKa = 11.84 WKK264 pKa = 10.54 AILGHH269 pKa = 7.42 DD270 pKa = 4.4 DD271 pKa = 3.4 GTIQGEE277 pKa = 4.24 NLFSVGSTSPIYY289 pKa = 10.47 NLNGDD294 pKa = 3.91 RR295 pKa = 11.84 LANNASDD302 pKa = 4.09 FFNGGMLNPIEE313 pKa = 4.15 YY314 pKa = 10.26 DD315 pKa = 3.31 EE316 pKa = 5.09 TGTSLGDD323 pKa = 3.43 VNVSVGLTQTGSNLLRR339 pKa = 11.84 QGDD342 pKa = 3.84 NTLGGNDD349 pKa = 3.88 FMGDD353 pKa = 2.94 GCSRR357 pKa = 11.84 GRR359 pKa = 11.84 SGQINKK365 pKa = 9.32 PAFAASISGDD375 pKa = 4.0 DD376 pKa = 4.14 FCDD379 pKa = 3.34 STPYY383 pKa = 10.27 PLYY386 pKa = 10.59 SVSPLLEE393 pKa = 4.24 VPSAPP398 pKa = 4.25
Molecular weight: 41.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.686
IPC2_protein 3.719
IPC_protein 3.757
Toseland 3.528
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.706
Rodwell 3.579
Grimsley 3.439
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.151
Thurlkill 3.579
EMBOSS 3.719
Sillero 3.884
Patrickios 0.998
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.784
Protein with the highest isoelectric point:
>tr|A0A1H2WHE6|A0A1H2WHE6_9ALTE Amino acid ABC transporter substrate-binding protein PAAT family (TC 3.A.1.3.-) OS=Marinobacter mobilis OX=488533 GN=SAMN04487960_104170 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 KK13 pKa = 7.97 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 8.88 ILARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.4 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3442
0
3442
1144372
29
2741
332.5
36.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.075 ± 0.046
0.975 ± 0.012
5.893 ± 0.027
6.272 ± 0.037
3.678 ± 0.028
7.849 ± 0.035
2.269 ± 0.022
5.071 ± 0.029
3.152 ± 0.028
10.945 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.489 ± 0.018
3.23 ± 0.024
4.795 ± 0.031
4.3 ± 0.031
6.609 ± 0.037
5.991 ± 0.025
5.119 ± 0.029
7.309 ± 0.034
1.369 ± 0.016
2.611 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here