Amino acid dipepetide frequency for Novosphingobium sp. PC22D

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.951AlaAla: 17.951 ± 0.167
1.208AlaCys: 1.208 ± 0.03
7.47AlaAsp: 7.47 ± 0.076
8.175AlaGlu: 8.175 ± 0.105
4.487AlaPhe: 4.487 ± 0.059
11.185AlaGly: 11.185 ± 0.108
2.339AlaHis: 2.339 ± 0.044
6.832AlaIle: 6.832 ± 0.074
3.797AlaLys: 3.797 ± 0.065
13.861AlaLeu: 13.861 ± 0.126
4.019AlaMet: 4.019 ± 0.064
3.049AlaAsn: 3.049 ± 0.048
5.963AlaPro: 5.963 ± 0.076
4.329AlaGln: 4.329 ± 0.059
10.448AlaArg: 10.448 ± 0.113
6.933AlaSer: 6.933 ± 0.07
5.96AlaThr: 5.96 ± 0.07
8.466AlaVal: 8.466 ± 0.097
1.787AlaTrp: 1.787 ± 0.037
2.605AlaTyr: 2.605 ± 0.039
0.0AlaXaa: 0.0 ± 0.0
Cys
1.15CysAla: 1.15 ± 0.028
0.109CysCys: 0.109 ± 0.01
0.618CysAsp: 0.618 ± 0.021
0.616CysGlu: 0.616 ± 0.021
0.322CysPhe: 0.322 ± 0.014
0.951CysGly: 0.951 ± 0.029
0.256CysHis: 0.256 ± 0.019
0.365CysIle: 0.365 ± 0.017
0.2CysLys: 0.2 ± 0.013
0.785CysLeu: 0.785 ± 0.024
0.179CysMet: 0.179 ± 0.011
0.227CysAsn: 0.227 ± 0.011
0.471CysPro: 0.471 ± 0.018
0.231CysGln: 0.231 ± 0.014
0.651CysArg: 0.651 ± 0.023
0.48CysSer: 0.48 ± 0.021
0.428CysThr: 0.428 ± 0.017
0.634CysVal: 0.634 ± 0.021
0.135CysTrp: 0.135 ± 0.011
0.2CysTyr: 0.2 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
7.66AspAla: 7.66 ± 0.07
0.588AspCys: 0.588 ± 0.021
3.519AspAsp: 3.519 ± 0.054
4.016AspGlu: 4.016 ± 0.056
2.314AspPhe: 2.314 ± 0.048
5.544AspGly: 5.544 ± 0.068
1.251AspHis: 1.251 ± 0.031
2.779AspIle: 2.779 ± 0.051
1.756AspLys: 1.756 ± 0.036
6.034AspLeu: 6.034 ± 0.073
1.449AspMet: 1.449 ± 0.034
1.322AspAsn: 1.322 ± 0.039
3.978AspPro: 3.978 ± 0.049
1.616AspGln: 1.616 ± 0.031
4.749AspArg: 4.749 ± 0.065
2.433AspSer: 2.433 ± 0.041
2.659AspThr: 2.659 ± 0.049
4.109AspVal: 4.109 ± 0.054
1.183AspTrp: 1.183 ± 0.031
1.66AspTyr: 1.66 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
8.897GluAla: 8.897 ± 0.095
0.434GluCys: 0.434 ± 0.019
3.369GluAsp: 3.369 ± 0.05
3.425GluGlu: 3.425 ± 0.06
1.835GluPhe: 1.835 ± 0.04
5.146GluGly: 5.146 ± 0.071
1.289GluHis: 1.289 ± 0.032
3.327GluIle: 3.327 ± 0.049
2.086GluLys: 2.086 ± 0.044
5.51GluLeu: 5.51 ± 0.057
1.532GluMet: 1.532 ± 0.037
1.498GluAsn: 1.498 ± 0.036
3.053GluPro: 3.053 ± 0.05
2.212GluGln: 2.212 ± 0.044
5.496GluArg: 5.496 ± 0.082
2.612GluSer: 2.612 ± 0.042
3.586GluThr: 3.586 ± 0.051
4.006GluVal: 4.006 ± 0.056
0.888GluTrp: 0.888 ± 0.027
1.118GluTyr: 1.118 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
5.095PheAla: 5.095 ± 0.06
0.359PheCys: 0.359 ± 0.016
2.817PheAsp: 2.817 ± 0.049
2.359PheGlu: 2.359 ± 0.039
1.285PhePhe: 1.285 ± 0.037
3.675PheGly: 3.675 ± 0.061
0.737PheHis: 0.737 ± 0.021
1.378PheIle: 1.378 ± 0.034
0.848PheLys: 0.848 ± 0.021
3.114PheLeu: 3.114 ± 0.049
0.721PheMet: 0.721 ± 0.022
0.904PheAsn: 0.904 ± 0.028
1.519PhePro: 1.519 ± 0.037
0.862PheGln: 0.862 ± 0.024
2.198PheArg: 2.198 ± 0.041
2.064PheSer: 2.064 ± 0.04
1.945PheThr: 1.945 ± 0.036
2.779PheVal: 2.779 ± 0.045
0.58PheTrp: 0.58 ± 0.022
0.946PheTyr: 0.946 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
10.031GlyAla: 10.031 ± 0.087
0.89GlyCys: 0.89 ± 0.026
4.86GlyAsp: 4.86 ± 0.068
5.64GlyGlu: 5.64 ± 0.068
3.622GlyPhe: 3.622 ± 0.046
7.978GlyGly: 7.978 ± 0.111
1.775GlyHis: 1.775 ± 0.035
4.326GlyIle: 4.326 ± 0.056
3.346GlyLys: 3.346 ± 0.055
8.659GlyLeu: 8.659 ± 0.083
2.392GlyMet: 2.392 ± 0.039
2.171GlyAsn: 2.171 ± 0.047
3.866GlyPro: 3.866 ± 0.057
2.708GlyGln: 2.708 ± 0.039
6.351GlyArg: 6.351 ± 0.079
4.868GlySer: 4.868 ± 0.08
4.815GlyThr: 4.815 ± 0.072
5.918GlyVal: 5.918 ± 0.067
1.56GlyTrp: 1.56 ± 0.035
2.431GlyTyr: 2.431 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
2.466HisAla: 2.466 ± 0.045
0.247HisCys: 0.247 ± 0.014
1.247HisAsp: 1.247 ± 0.028
1.208HisGlu: 1.208 ± 0.03
0.866HisPhe: 0.866 ± 0.022
1.946HisGly: 1.946 ± 0.038
0.559HisHis: 0.559 ± 0.019
0.819HisIle: 0.819 ± 0.025
0.471HisLys: 0.471 ± 0.019
1.876HisLeu: 1.876 ± 0.037
0.463HisMet: 0.463 ± 0.019
0.437HisAsn: 0.437 ± 0.018
1.226HisPro: 1.226 ± 0.03
0.526HisGln: 0.526 ± 0.021
1.511HisArg: 1.511 ± 0.031
0.905HisSer: 0.905 ± 0.026
0.744HisThr: 0.744 ± 0.022
1.489HisVal: 1.489 ± 0.033
0.377HisTrp: 0.377 ± 0.017
0.575HisTyr: 0.575 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
7.609IleAla: 7.609 ± 0.075
0.453IleCys: 0.453 ± 0.017
3.841IleAsp: 3.841 ± 0.052
3.882IleGlu: 3.882 ± 0.056
1.488IlePhe: 1.488 ± 0.034
4.765IleGly: 4.765 ± 0.055
0.852IleHis: 0.852 ± 0.025
1.704IleIle: 1.704 ± 0.035
1.166IleLys: 1.166 ± 0.029
3.767IleLeu: 3.767 ± 0.057
0.873IleMet: 0.873 ± 0.028
1.204IleAsn: 1.204 ± 0.031
2.172IlePro: 2.172 ± 0.04
1.059IleGln: 1.059 ± 0.029
3.053IleArg: 3.053 ± 0.045
2.597IleSer: 2.597 ± 0.043
2.318IleThr: 2.318 ± 0.042
4.092IleVal: 4.092 ± 0.055
0.62IleTrp: 0.62 ± 0.02
1.005IleTyr: 1.005 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
3.915LysAla: 3.915 ± 0.055
0.171LysCys: 0.171 ± 0.01
1.551LysAsp: 1.551 ± 0.036
1.282LysGlu: 1.282 ± 0.035
0.827LysPhe: 0.827 ± 0.028
2.536LysGly: 2.536 ± 0.047
0.534LysHis: 0.534 ± 0.02
1.396LysIle: 1.396 ± 0.032
0.919LysLys: 0.919 ± 0.028
3.057LysLeu: 3.057 ± 0.054
0.673LysMet: 0.673 ± 0.023
0.632LysAsn: 0.632 ± 0.022
1.841LysPro: 1.841 ± 0.036
0.888LysGln: 0.888 ± 0.025
2.263LysArg: 2.263 ± 0.041
1.627LysSer: 1.627 ± 0.033
1.603LysThr: 1.603 ± 0.031
2.225LysVal: 2.225 ± 0.04
0.375LysTrp: 0.375 ± 0.018
0.585LysTyr: 0.585 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
14.647LeuAla: 14.647 ± 0.121
0.895LeuCys: 0.895 ± 0.029
6.369LeuAsp: 6.369 ± 0.068
5.572LeuGlu: 5.572 ± 0.069
3.483LeuPhe: 3.483 ± 0.055
8.508LeuGly: 8.508 ± 0.081
1.877LeuHis: 1.877 ± 0.035
4.385LeuIle: 4.385 ± 0.059
2.804LeuLys: 2.804 ± 0.048
9.205LeuLeu: 9.205 ± 0.11
2.051LeuMet: 2.051 ± 0.037
2.144LeuAsn: 2.144 ± 0.041
5.315LeuPro: 5.315 ± 0.065
2.329LeuGln: 2.329 ± 0.048
7.15LeuArg: 7.15 ± 0.081
5.923LeuSer: 5.923 ± 0.06
5.236LeuThr: 5.236 ± 0.066
7.513LeuVal: 7.513 ± 0.081
1.223LeuTrp: 1.223 ± 0.033
1.865LeuTyr: 1.865 ± 0.035
0.0LeuXaa: 0.0 ± 0.0
Met
3.298MetAla: 3.298 ± 0.05
0.181MetCys: 0.181 ± 0.011
1.169MetAsp: 1.169 ± 0.029
1.158MetGlu: 1.158 ± 0.027
0.688MetPhe: 0.688 ± 0.021
1.876MetGly: 1.876 ± 0.038
0.486MetHis: 0.486 ± 0.018
1.292MetIle: 1.292 ± 0.029
0.86MetLys: 0.86 ± 0.025
2.62MetLeu: 2.62 ± 0.042
0.662MetMet: 0.662 ± 0.022
0.687MetAsn: 0.687 ± 0.021
1.526MetPro: 1.526 ± 0.029
0.754MetGln: 0.754 ± 0.023
1.91MetArg: 1.91 ± 0.037
1.503MetSer: 1.503 ± 0.027
1.75MetThr: 1.75 ± 0.037
1.607MetVal: 1.607 ± 0.038
0.212MetTrp: 0.212 ± 0.012
0.256MetTyr: 0.256 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.104AsnAla: 3.104 ± 0.049
0.252AsnCys: 0.252 ± 0.013
1.338AsnAsp: 1.338 ± 0.032
1.189AsnGlu: 1.189 ± 0.029
0.953AsnPhe: 0.953 ± 0.026
2.201AsnGly: 2.201 ± 0.047
0.446AsnHis: 0.446 ± 0.019
1.112AsnIle: 1.112 ± 0.031
0.59AsnLys: 0.59 ± 0.022
2.387AsnLeu: 2.387 ± 0.043
0.533AsnMet: 0.533 ± 0.019
0.584AsnAsn: 0.584 ± 0.024
1.697AsnPro: 1.697 ± 0.032
0.67AsnGln: 0.67 ± 0.024
1.781AsnArg: 1.781 ± 0.037
1.177AsnSer: 1.177 ± 0.031
1.093AsnThr: 1.093 ± 0.028
1.814AsnVal: 1.814 ± 0.038
0.432AsnTrp: 0.432 ± 0.019
0.664AsnTyr: 0.664 ± 0.025
0.0AsnXaa: 0.0 ± 0.0
Pro
6.577ProAla: 6.577 ± 0.083
0.363ProCys: 0.363 ± 0.017
3.866ProAsp: 3.866 ± 0.056
4.121ProGlu: 4.121 ± 0.055
1.844ProPhe: 1.844 ± 0.036
4.983ProGly: 4.983 ± 0.071
1.041ProHis: 1.041 ± 0.028
2.356ProIle: 2.356 ± 0.039
1.364ProLys: 1.364 ± 0.032
4.88ProLeu: 4.88 ± 0.069
1.253ProMet: 1.253 ± 0.031
1.138ProAsn: 1.138 ± 0.028
2.738ProPro: 2.738 ± 0.062
1.641ProGln: 1.641 ± 0.03
3.225ProArg: 3.225 ± 0.049
2.791ProSer: 2.791 ± 0.041
2.321ProThr: 2.321 ± 0.073
4.155ProVal: 4.155 ± 0.064
0.681ProTrp: 0.681 ± 0.024
1.074ProTyr: 1.074 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
3.946GlnAla: 3.946 ± 0.061
0.275GlnCys: 0.275 ± 0.016
1.425GlnAsp: 1.425 ± 0.031
1.411GlnGlu: 1.411 ± 0.033
1.11GlnPhe: 1.11 ± 0.03
2.411GlnGly: 2.411 ± 0.04
0.543GlnHis: 0.543 ± 0.02
1.598GlnIle: 1.598 ± 0.033
0.81GlnLys: 0.81 ± 0.024
2.867GlnLeu: 2.867 ± 0.049
0.783GlnMet: 0.783 ± 0.025
0.7GlnAsn: 0.7 ± 0.024
1.601GlnPro: 1.601 ± 0.035
1.047GlnGln: 1.047 ± 0.038
2.288GlnArg: 2.288 ± 0.048
1.661GlnSer: 1.661 ± 0.035
1.52GlnThr: 1.52 ± 0.033
2.279GlnVal: 2.279 ± 0.043
0.436GlnTrp: 0.436 ± 0.017
0.624GlnTyr: 0.624 ± 0.02
0.0GlnXaa: 0.0 ± 0.0
Arg
8.882ArgAla: 8.882 ± 0.102
0.567ArgCys: 0.567 ± 0.023
4.499ArgAsp: 4.499 ± 0.064
5.038ArgGlu: 5.038 ± 0.073
3.231ArgPhe: 3.231 ± 0.046
5.257ArgGly: 5.257 ± 0.07
1.775ArgHis: 1.775 ± 0.039
4.258ArgIle: 4.258 ± 0.058
2.424ArgLys: 2.424 ± 0.04
8.102ArgLeu: 8.102 ± 0.097
1.945ArgMet: 1.945 ± 0.04
1.868ArgAsn: 1.868 ± 0.035
3.616ArgPro: 3.616 ± 0.055
2.442ArgGln: 2.442 ± 0.04
5.914ArgArg: 5.914 ± 0.087
3.85ArgSer: 3.85 ± 0.053
3.473ArgThr: 3.473 ± 0.048
4.766ArgVal: 4.766 ± 0.063
1.174ArgTrp: 1.174 ± 0.033
1.923ArgTyr: 1.923 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
6.435SerAla: 6.435 ± 0.082
0.442SerCys: 0.442 ± 0.019
3.318SerAsp: 3.318 ± 0.058
3.097SerGlu: 3.097 ± 0.045
2.026SerPhe: 2.026 ± 0.042
5.627SerGly: 5.627 ± 0.07
1.063SerHis: 1.063 ± 0.025
2.49SerIle: 2.49 ± 0.047
1.356SerLys: 1.356 ± 0.032
5.35SerLeu: 5.35 ± 0.071
1.272SerMet: 1.272 ± 0.029
1.317SerAsn: 1.317 ± 0.035
2.924SerPro: 2.924 ± 0.048
1.644SerGln: 1.644 ± 0.032
3.926SerArg: 3.926 ± 0.051
2.881SerSer: 2.881 ± 0.057
2.508SerThr: 2.508 ± 0.049
3.652SerVal: 3.652 ± 0.058
0.792SerTrp: 0.792 ± 0.024
1.382SerTyr: 1.382 ± 0.033
0.0SerXaa: 0.0 ± 0.0
Thr
5.834ThrAla: 5.834 ± 0.072
0.457ThrCys: 0.457 ± 0.02
2.898ThrAsp: 2.898 ± 0.046
2.507ThrGlu: 2.507 ± 0.044
1.841ThrPhe: 1.841 ± 0.03
5.161ThrGly: 5.161 ± 0.067
0.917ThrHis: 0.917 ± 0.025
2.896ThrIle: 2.896 ± 0.047
1.259ThrLys: 1.259 ± 0.033
5.386ThrLeu: 5.386 ± 0.06
1.279ThrMet: 1.279 ± 0.031
1.17ThrAsn: 1.17 ± 0.03
3.12ThrPro: 3.12 ± 0.074
1.335ThrGln: 1.335 ± 0.029
3.548ThrArg: 3.548 ± 0.054
2.716ThrSer: 2.716 ± 0.045
2.459ThrThr: 2.459 ± 0.045
3.979ThrVal: 3.979 ± 0.06
0.677ThrTrp: 0.677 ± 0.021
1.2ThrTyr: 1.2 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
9.292ValAla: 9.292 ± 0.093
0.663ValCys: 0.663 ± 0.021
4.108ValAsp: 4.108 ± 0.055
4.663ValGlu: 4.663 ± 0.055
2.462ValPhe: 2.462 ± 0.042
5.397ValGly: 5.397 ± 0.067
1.361ValHis: 1.361 ± 0.029
3.641ValIle: 3.641 ± 0.055
1.824ValLys: 1.824 ± 0.041
7.023ValLeu: 7.023 ± 0.074
1.644ValMet: 1.644 ± 0.035
1.932ValAsn: 1.932 ± 0.039
3.981ValPro: 3.981 ± 0.058
1.778ValGln: 1.778 ± 0.033
5.228ValArg: 5.228 ± 0.057
4.306ValSer: 4.306 ± 0.05
4.301ValThr: 4.301 ± 0.053
5.38ValVal: 5.38 ± 0.076
0.946ValTrp: 0.946 ± 0.028
1.42ValTyr: 1.42 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
1.333TrpAla: 1.333 ± 0.033
0.16TrpCys: 0.16 ± 0.009
0.802TrpAsp: 0.802 ± 0.025
0.634TrpGlu: 0.634 ± 0.023
0.626TrpPhe: 0.626 ± 0.02
0.969TrpGly: 0.969 ± 0.024
0.369TrpHis: 0.369 ± 0.015
0.766TrpIle: 0.766 ± 0.02
0.465TrpLys: 0.465 ± 0.02
1.84TrpLeu: 1.84 ± 0.041
0.355TrpMet: 0.355 ± 0.016
0.438TrpAsn: 0.438 ± 0.017
0.73TrpPro: 0.73 ± 0.021
0.627TrpGln: 0.627 ± 0.019
1.413TrpArg: 1.413 ± 0.03
0.963TrpSer: 0.963 ± 0.027
0.846TrpThr: 0.846 ± 0.025
0.829TrpVal: 0.829 ± 0.026
0.242TrpTrp: 0.242 ± 0.014
0.289TrpTyr: 0.289 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.741TyrAla: 2.741 ± 0.043
0.294TyrCys: 0.294 ± 0.013
1.536TyrAsp: 1.536 ± 0.037
1.326TyrGlu: 1.326 ± 0.031
0.872TyrPhe: 0.872 ± 0.025
2.093TyrGly: 2.093 ± 0.037
0.488TyrHis: 0.488 ± 0.019
0.799TyrIle: 0.799 ± 0.025
0.557TyrLys: 0.557 ± 0.022
2.174TyrLeu: 2.174 ± 0.045
0.418TyrMet: 0.418 ± 0.018
0.595TyrAsn: 0.595 ± 0.025
1.076TyrPro: 1.076 ± 0.028
0.636TyrGln: 0.636 ± 0.021
1.901TyrArg: 1.901 ± 0.031
1.21TyrSer: 1.21 ± 0.035
1.073TyrThr: 1.073 ± 0.03
1.658TyrVal: 1.658 ± 0.037
0.376TyrTrp: 0.376 ± 0.017
0.661TyrTyr: 0.661 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4593 proteins (1478106 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski