Stenotrophomonas phage vB_SmaS_DLP_5
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 149 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D2W2X7|A0A2D2W2X7_9CAUD Uncharacterized protein OS=Stenotrophomonas phage vB_SmaS_DLP_5 OX=2044561 GN=DLP05_070 PE=4 SV=1
MM1 pKa = 7.6 SDD3 pKa = 3.78 EE4 pKa = 3.95 GDD6 pKa = 3.05 MWVAYY11 pKa = 10.17 AHH13 pKa = 6.29 TYY15 pKa = 9.67 EE16 pKa = 4.12 EE17 pKa = 4.66 AVAKK21 pKa = 10.08 SLPNFPDD28 pKa = 3.53 GGRR31 pKa = 11.84 VEE33 pKa = 5.04 SVDD36 pKa = 3.23 RR37 pKa = 11.84 DD38 pKa = 3.57 ARR40 pKa = 11.84 LDD42 pKa = 3.59 KK43 pKa = 10.86 YY44 pKa = 11.45 SPGPAPMWEE53 pKa = 4.29 LLALAYY59 pKa = 9.93 EE60 pKa = 4.87 WVCLNCGALVNEE72 pKa = 4.82 DD73 pKa = 3.92 LFDD76 pKa = 5.15 AEE78 pKa = 5.04 EE79 pKa = 3.93 EE80 pKa = 4.46 RR81 pKa = 11.84 EE82 pKa = 4.24 LKK84 pKa = 10.55 PVFDD88 pKa = 6.46 DD89 pKa = 4.89 DD90 pKa = 3.87 MQLVFCCPEE99 pKa = 3.7 CAIEE103 pKa = 4.05 YY104 pKa = 9.83 EE105 pKa = 4.23 RR106 pKa = 11.84 EE107 pKa = 3.75 QRR109 pKa = 11.84 ATFHH113 pKa = 6.72
Molecular weight: 12.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.851
IPC2_protein 4.101
IPC_protein 4.037
Toseland 3.859
ProMoST 4.151
Dawson 3.999
Bjellqvist 4.151
Wikipedia 3.884
Rodwell 3.872
Grimsley 3.77
Solomon 3.986
Lehninger 3.948
Nozaki 4.113
DTASelect 4.266
Thurlkill 3.884
EMBOSS 3.897
Sillero 4.151
Patrickios 1.926
IPC_peptide 3.999
IPC2_peptide 4.139
IPC2.peptide.svr19 4.047
Protein with the highest isoelectric point:
>tr|A0A2D2W2R2|A0A2D2W2R2_9CAUD Uncharacterized protein OS=Stenotrophomonas phage vB_SmaS_DLP_5 OX=2044561 GN=DLP05_122 PE=4 SV=1
MM1 pKa = 6.98 ATRR4 pKa = 11.84 RR5 pKa = 11.84 APVKK9 pKa = 10.02 TPTNVKK15 pKa = 10.59 APTEE19 pKa = 4.13 TVNQKK24 pKa = 9.65 VKK26 pKa = 10.62 PGLLKK31 pKa = 10.83 ASSNTNQRR39 pKa = 11.84 SSTPSIRR46 pKa = 11.84 ANTTRR51 pKa = 11.84 TTTTKK56 pKa = 10.9
Molecular weight: 6.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.239
IPC2_protein 10.511
IPC_protein 12.018
Toseland 12.208
ProMoST 12.676
Dawson 12.223
Bjellqvist 12.193
Wikipedia 12.676
Rodwell 12.149
Grimsley 12.252
Solomon 12.691
Lehninger 12.588
Nozaki 12.208
DTASelect 12.193
Thurlkill 12.208
EMBOSS 12.705
Sillero 12.208
Patrickios 11.901
IPC_peptide 12.691
IPC2_peptide 11.667
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
149
0
149
30569
32
1251
205.2
22.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.895 ± 0.469
1.063 ± 0.102
5.692 ± 0.128
7.04 ± 0.21
3.68 ± 0.134
7.727 ± 0.281
1.937 ± 0.137
4.662 ± 0.145
6.909 ± 0.287
8.322 ± 0.235
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.301 ± 0.117
3.909 ± 0.162
4.439 ± 0.151
4.105 ± 0.203
4.789 ± 0.174
5.558 ± 0.186
5.793 ± 0.176
7.586 ± 0.188
1.472 ± 0.095
3.121 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here