Marinilabiliaceae bacterium JC017
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4913 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q8RMT9|A0A4Q8RMT9_9BACT 4-hydroxy-tetrahydrodipicolinate synthase OS=Marinilabiliaceae bacterium JC017 OX=2234116 GN=dapA PE=3 SV=1
MM1 pKa = 7.5 KK2 pKa = 10.2 ALKK5 pKa = 10.21 FGLLIALVGLFTVSCDD21 pKa = 4.4 DD22 pKa = 5.03 DD23 pKa = 6.28 DD24 pKa = 6.26 DD25 pKa = 5.12 YY26 pKa = 12.23 SLGKK30 pKa = 10.07 FIISMGTIEE39 pKa = 5.19 GDD41 pKa = 2.94 QDD43 pKa = 3.89 SYY45 pKa = 11.79 IIVTDD50 pKa = 3.17 SGDD53 pKa = 3.56 RR54 pKa = 11.84 LFPSASNVPNYY65 pKa = 9.71 PVEE68 pKa = 5.33 DD69 pKa = 4.13 DD70 pKa = 3.2 MRR72 pKa = 11.84 VWVNYY77 pKa = 8.8 TILGDD82 pKa = 3.61 ATDD85 pKa = 4.98 NEE87 pKa = 4.44 TLDD90 pKa = 3.98 YY91 pKa = 10.39 YY92 pKa = 11.49 VKK94 pKa = 10.67 INDD97 pKa = 4.06 LSDD100 pKa = 3.57 VLTKK104 pKa = 10.6 DD105 pKa = 3.05 IFQLTDD111 pKa = 3.06 ATRR114 pKa = 11.84 DD115 pKa = 3.67 SIGNDD120 pKa = 3.17 PVSISDD126 pKa = 3.12 YY127 pKa = 10.34 WIARR131 pKa = 11.84 DD132 pKa = 3.49 YY133 pKa = 11.72 LNIGFYY139 pKa = 10.82 YY140 pKa = 10.61 GGGPGYY146 pKa = 9.58 IHH148 pKa = 7.19 YY149 pKa = 10.39 INLVNDD155 pKa = 3.54 EE156 pKa = 4.82 NDD158 pKa = 3.53 PEE160 pKa = 4.54 TEE162 pKa = 4.03 DD163 pKa = 3.89 GMLILEE169 pKa = 4.9 LKK171 pKa = 9.87 HH172 pKa = 6.36 NKK174 pKa = 9.67 NNDD177 pKa = 3.26 PYY179 pKa = 11.39 NYY181 pKa = 9.96 RR182 pKa = 11.84 INNWASFDD190 pKa = 3.83 LLSIQEE196 pKa = 4.07 EE197 pKa = 4.73 GKK199 pKa = 10.71 DD200 pKa = 3.38 EE201 pKa = 4.24 VTFLLRR207 pKa = 11.84 SIGPSGEE214 pKa = 4.08 YY215 pKa = 10.19 EE216 pKa = 3.99 FEE218 pKa = 4.31 KK219 pKa = 10.8 EE220 pKa = 3.81 VTYY223 pKa = 10.5 KK224 pKa = 10.99 YY225 pKa = 11.12 GLDD228 pKa = 3.46 LL229 pKa = 4.98
Molecular weight: 26.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.706
IPC2_protein 3.872
IPC_protein 3.884
Toseland 3.668
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.834
Rodwell 3.706
Grimsley 3.567
Solomon 3.884
Lehninger 3.834
Nozaki 3.999
DTASelect 4.266
Thurlkill 3.719
EMBOSS 3.834
Sillero 4.012
Patrickios 1.252
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.893
Protein with the highest isoelectric point:
>tr|A0A4Q8RRE7|A0A4Q8RRE7_9BACT Acetate kinase OS=Marinilabiliaceae bacterium JC017 OX=2234116 GN=ackA PE=3 SV=1
MM1 pKa = 7.31 VILPRR6 pKa = 11.84 GMVILPRR13 pKa = 11.84 GMVILPRR20 pKa = 11.84 GMVILPRR27 pKa = 11.84 GMVILPRR34 pKa = 11.84 GMVILPRR41 pKa = 11.84 GMVILPRR48 pKa = 11.84 GMVILPRR55 pKa = 11.84 GTVILPRR62 pKa = 11.84 GTVILPRR69 pKa = 11.84 GTVILPRR76 pKa = 11.84 CMDD79 pKa = 3.85 ILPRR83 pKa = 11.84 GMIILPRR90 pKa = 11.84 GMNVLPNGIIILPKK104 pKa = 10.51 NFIIRR109 pKa = 3.63
Molecular weight: 12.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.369
IPC2_protein 10.789
IPC_protein 12.398
Toseland 12.559
ProMoST 13.071
Dawson 12.559
Bjellqvist 12.559
Wikipedia 13.042
Rodwell 12.106
Grimsley 12.603
Solomon 13.071
Lehninger 12.969
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.071
Sillero 12.559
Patrickios 11.842
IPC_peptide 13.071
IPC2_peptide 12.062
IPC2.peptide.svr19 9.172
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4913
0
4913
1713457
14
6519
348.8
39.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.318 ± 0.033
0.994 ± 0.012
5.52 ± 0.033
6.566 ± 0.031
4.988 ± 0.032
6.666 ± 0.038
2.033 ± 0.019
7.37 ± 0.034
7.221 ± 0.038
9.347 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.446 ± 0.018
5.583 ± 0.032
3.65 ± 0.017
3.602 ± 0.02
4.121 ± 0.026
6.342 ± 0.026
5.354 ± 0.038
6.445 ± 0.036
1.23 ± 0.015
4.162 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here