Ferrimonas sp. IMCC35002
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4149 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V5NYR8|A0A4V5NYR8_9GAMM HD domain-containing protein OS=Ferrimonas sp. IMCC35002 OX=2569539 GN=FCL42_08205 PE=4 SV=1
MM1 pKa = 7.73 HH2 pKa = 7.23 YY3 pKa = 8.38 NTSEE7 pKa = 4.01 LCDD10 pKa = 4.33 LYY12 pKa = 11.31 AEE14 pKa = 4.2 QVDD17 pKa = 4.32 VVEE20 pKa = 5.02 PMFTNFGGCDD30 pKa = 3.14 SFGGEE35 pKa = 4.45 LQTIKK40 pKa = 10.73 CFEE43 pKa = 4.75 DD44 pKa = 3.25 SVLIAEE50 pKa = 4.55 TLEE53 pKa = 3.79 QDD55 pKa = 3.49 GTGKK59 pKa = 10.29 VLLVDD64 pKa = 4.04 GGGSLRR70 pKa = 11.84 RR71 pKa = 11.84 ALIDD75 pKa = 3.37 AKK77 pKa = 10.14 MAEE80 pKa = 4.61 LAAQNNWEE88 pKa = 3.95 GLIVYY93 pKa = 9.28 GSVRR97 pKa = 11.84 EE98 pKa = 3.88 VDD100 pKa = 3.47 ALVDD104 pKa = 3.44 IDD106 pKa = 5.15 IGIQALASIPVGAYY120 pKa = 6.73 QTGEE124 pKa = 4.11 GAVDD128 pKa = 3.49 VPVNFGGVTFLPEE141 pKa = 3.5 DD142 pKa = 4.26 HH143 pKa = 7.27 IYY145 pKa = 11.27 ADD147 pKa = 3.48 STGIILSPDD156 pKa = 3.9 PLDD159 pKa = 4.64 LDD161 pKa = 3.92
Molecular weight: 17.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.694
IPC_protein 3.681
Toseland 3.465
ProMoST 3.795
Dawson 3.668
Bjellqvist 3.872
Wikipedia 3.605
Rodwell 3.503
Grimsley 3.376
Solomon 3.656
Lehninger 3.617
Nozaki 3.795
DTASelect 4.012
Thurlkill 3.528
EMBOSS 3.617
Sillero 3.795
Patrickios 0.947
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.759
Protein with the highest isoelectric point:
>tr|A0A4U1BK45|A0A4U1BK45_9GAMM Arginine N-succinyltransferase OS=Ferrimonas sp. IMCC35002 OX=2569539 GN=astA PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 9.37 RR14 pKa = 11.84 NHH16 pKa = 5.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.32 AGRR28 pKa = 11.84 QILARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.4 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4149
0
4149
1391800
24
5040
335.5
37.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.409 ± 0.045
1.062 ± 0.014
5.601 ± 0.037
6.031 ± 0.032
3.763 ± 0.024
7.35 ± 0.04
2.33 ± 0.022
5.356 ± 0.033
4.453 ± 0.035
11.029 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.655 ± 0.021
3.758 ± 0.027
4.239 ± 0.023
5.385 ± 0.041
4.936 ± 0.03
6.429 ± 0.031
5.138 ± 0.028
6.846 ± 0.037
1.387 ± 0.016
2.844 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here