Aedes pseudoscutellaris reovirus (isolate France) (ApRV)
Average proteome isoelectric point is 5.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q2Y0E5|VP6_APRVF Microtubule-associated protein VP6 OS=Aedes pseudoscutellaris reovirus (isolate France) OX=648170 GN=S6 PE=3 SV=1
MM1 pKa = 7.88 AFTYY5 pKa = 9.37 WDD7 pKa = 3.35 KK8 pKa = 11.12 EE9 pKa = 3.62 KK10 pKa = 11.14 RR11 pKa = 11.84 MTLKK15 pKa = 10.73 QMIQQVTINEE25 pKa = 4.16 QEE27 pKa = 4.08 NEE29 pKa = 3.83 LTHH32 pKa = 6.37 YY33 pKa = 11.04 VFTTPMSMPTFGKK46 pKa = 9.77 PMLGYY51 pKa = 10.58 VPLNEE56 pKa = 4.1 VATSKK61 pKa = 10.61 FFSNVNDD68 pKa = 3.88 FDD70 pKa = 5.41 RR71 pKa = 11.84 DD72 pKa = 3.55 NQLAMAHH79 pKa = 5.89 FQDD82 pKa = 3.94 TTITRR87 pKa = 11.84 AYY89 pKa = 10.74 NLTNSIKK96 pKa = 10.7 PGDD99 pKa = 3.81 TSLPDD104 pKa = 3.53 AEE106 pKa = 4.4 VAALKK111 pKa = 9.51 WFWKK115 pKa = 10.18 FFTSINLVRR124 pKa = 11.84 QPPMDD129 pKa = 3.58 NVLYY133 pKa = 8.94 WACQFLSSGTSFLPLEE149 pKa = 4.51 KK150 pKa = 10.37 DD151 pKa = 3.53 VEE153 pKa = 4.56 IVFSGFQGSHH163 pKa = 3.79 TCMFASLRR171 pKa = 11.84 QMNLSPILCPYY182 pKa = 9.03 YY183 pKa = 10.74 EE184 pKa = 4.58 HH185 pKa = 6.31 MTNFKK190 pKa = 7.62 TTAEE194 pKa = 3.55 IRR196 pKa = 11.84 EE197 pKa = 4.08 YY198 pKa = 11.41 VDD200 pKa = 3.84 ANEE203 pKa = 4.24 EE204 pKa = 4.2 LKK206 pKa = 11.15 SLITYY211 pKa = 9.65 LCLCTIVGLCDD222 pKa = 3.37 TFVEE226 pKa = 4.53 TRR228 pKa = 11.84 NMEE231 pKa = 3.87 TGEE234 pKa = 4.21 YY235 pKa = 7.84 VWKK238 pKa = 10.32 VSNVVSDD245 pKa = 3.52 NHH247 pKa = 6.38 TPAQNIGKK255 pKa = 9.29 FCYY258 pKa = 7.27 TTQNAKK264 pKa = 10.68 YY265 pKa = 8.58 MIQLVHH271 pKa = 6.43 VLLFPLTDD279 pKa = 3.2 NKK281 pKa = 10.92 YY282 pKa = 10.85 ADD284 pKa = 4.05 LPNYY288 pKa = 9.41 VAVITQGAINQSLPRR303 pKa = 11.84 NVVNNNEE310 pKa = 4.21 GSNSDD315 pKa = 3.65 TTTDD319 pKa = 3.68 TAPSTSGIVSGSADD333 pKa = 3.66 TVASLYY339 pKa = 10.06 PDD341 pKa = 3.19 EE342 pKa = 4.85 FKK344 pKa = 11.14 YY345 pKa = 10.59 VQSS348 pKa = 3.71
Molecular weight: 39.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.692
IPC2_protein 4.889
IPC_protein 4.825
Toseland 4.724
ProMoST 4.952
Dawson 4.8
Bjellqvist 4.94
Wikipedia 4.673
Rodwell 4.698
Grimsley 4.635
Solomon 4.8
Lehninger 4.749
Nozaki 4.914
DTASelect 5.08
Thurlkill 4.724
EMBOSS 4.711
Sillero 4.978
Patrickios 2.969
IPC_peptide 4.8
IPC2_peptide 4.978
IPC2.peptide.svr19 4.894
Protein with the highest isoelectric point:
>sp|Q2Y0F0|VP1_APRVF Putative structural protein VP1 OS=Aedes pseudoscutellaris reovirus (isolate France) OX=648170 GN=S1 PE=4 SV=1
MM1 pKa = 7.09 ATEE4 pKa = 4.11 PNEE7 pKa = 3.76 INLRR11 pKa = 11.84 MTQTRR16 pKa = 11.84 MKK18 pKa = 10.27 QIEE21 pKa = 4.38 DD22 pKa = 3.52 TTFKK26 pKa = 10.33 IRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 9.69 HH31 pKa = 6.6 DD32 pKa = 3.29 DD33 pKa = 3.22 MYY35 pKa = 11.13 TYY37 pKa = 10.67 LINDD41 pKa = 3.82 IEE43 pKa = 4.27 ATEE46 pKa = 4.5 MYY48 pKa = 9.59 PDD50 pKa = 3.63 TEE52 pKa = 4.4 TPFRR56 pKa = 11.84 MKK58 pKa = 10.69 SSLNNTYY65 pKa = 10.06 IQVKK69 pKa = 9.12 CQIPKK74 pKa = 10.74 LIMQPPLYY82 pKa = 10.51 NNDD85 pKa = 3.65 MYY87 pKa = 11.11 PLNHH91 pKa = 6.64 NSTNFLEE98 pKa = 4.85 DD99 pKa = 3.04 RR100 pKa = 11.84 PFNYY104 pKa = 10.27 LCNFDD109 pKa = 3.27 WFEE112 pKa = 3.86 FLSRR116 pKa = 11.84 SKK118 pKa = 11.32 DD119 pKa = 3.65 EE120 pKa = 5.71 LGMHH124 pKa = 6.49 YY125 pKa = 11.37 NMIRR129 pKa = 11.84 DD130 pKa = 5.0 LISMSSQTRR139 pKa = 11.84 YY140 pKa = 10.73 SNIWSNISGIIMYY153 pKa = 9.81 LRR155 pKa = 11.84 QYY157 pKa = 11.03 KK158 pKa = 10.28 RR159 pKa = 11.84 GFALRR164 pKa = 11.84 SILIRR169 pKa = 11.84 LLTYY173 pKa = 9.68 WNNFPFFDD181 pKa = 3.54 TWDD184 pKa = 3.48 GFKK187 pKa = 10.96 DD188 pKa = 3.76 VLPKK192 pKa = 10.59 TSTAWPLLFYY202 pKa = 11.11 AFMSVAFDD210 pKa = 4.09 YY211 pKa = 10.67 ICEE214 pKa = 4.42 NISEE218 pKa = 4.34 GDD220 pKa = 4.19 AIVCFQNHH228 pKa = 6.42 LEE230 pKa = 4.11 NAQISYY236 pKa = 11.06 QDD238 pKa = 3.21 TKK240 pKa = 11.35 LEE242 pKa = 4.15 KK243 pKa = 10.72 KK244 pKa = 10.35 NGYY247 pKa = 6.55 TLWLKK252 pKa = 9.43 YY253 pKa = 9.35 EE254 pKa = 4.11 CHH256 pKa = 6.37 NLRR259 pKa = 11.84 VALTPRR265 pKa = 11.84 YY266 pKa = 9.79 DD267 pKa = 3.65 YY268 pKa = 11.32 TGTVYY273 pKa = 11.08 GNFLKK278 pKa = 10.22 DD279 pKa = 3.1 TTEE282 pKa = 4.09 YY283 pKa = 10.45 IITDD287 pKa = 3.38 EE288 pKa = 4.52 FEE290 pKa = 4.06 EE291 pKa = 5.05 NEE293 pKa = 3.93 IKK295 pKa = 10.1 MKK297 pKa = 10.9 NILNPSFYY305 pKa = 10.55 KK306 pKa = 10.34 ILQQLRR312 pKa = 11.84 RR313 pKa = 11.84 EE314 pKa = 4.19 IKK316 pKa = 8.87 TVEE319 pKa = 4.34 DD320 pKa = 4.31 IIRR323 pKa = 11.84 LLTICYY329 pKa = 9.59 SARR332 pKa = 11.84 DD333 pKa = 3.51 DD334 pKa = 3.36 RR335 pKa = 11.84 TYY337 pKa = 11.33 YY338 pKa = 8.68 GTLMEE343 pKa = 4.62 LAISKK348 pKa = 10.18 AIKK351 pKa = 9.05 PQVAGSIVPKK361 pKa = 9.87 PIPTSWLQKK370 pKa = 10.07 DD371 pKa = 3.65 PKK373 pKa = 10.21 IVLSAKK379 pKa = 10.07 YY380 pKa = 9.4 PSTSFLSQMQEE391 pKa = 3.79 FYY393 pKa = 10.85 RR394 pKa = 11.84 RR395 pKa = 11.84 YY396 pKa = 8.95 YY397 pKa = 9.87 PALQRR402 pKa = 11.84 EE403 pKa = 4.34 IDD405 pKa = 3.77 VHH407 pKa = 7.22 ALSASFINFLSTASAGVGIEE427 pKa = 4.03 LPEE430 pKa = 4.44 EE431 pKa = 4.45 IINMVKK437 pKa = 10.48 DD438 pKa = 3.38 KK439 pKa = 11.17 RR440 pKa = 11.84 LLYY443 pKa = 10.28 LLKK446 pKa = 10.31 KK447 pKa = 10.61 GSGKK451 pKa = 10.12 RR452 pKa = 11.84 VLQEE456 pKa = 3.56 ALLVEE461 pKa = 5.26 RR462 pKa = 11.84 YY463 pKa = 10.0 NDD465 pKa = 4.12 LDD467 pKa = 4.44 PIINDD472 pKa = 3.63 FVRR475 pKa = 11.84 LIKK478 pKa = 10.36 IVVRR482 pKa = 11.84 KK483 pKa = 9.76 QIEE486 pKa = 3.82 RR487 pKa = 11.84 RR488 pKa = 11.84 QRR490 pKa = 11.84 GIAGIPNNVLKK501 pKa = 10.56 INQVTYY507 pKa = 10.19 EE508 pKa = 4.02 ANKK511 pKa = 9.46 PFSKK515 pKa = 10.12 IARR518 pKa = 11.84 APSHH522 pKa = 6.36 GKK524 pKa = 9.8 QSGNASDD531 pKa = 3.42 IHH533 pKa = 7.15 DD534 pKa = 4.26 LLFYY538 pKa = 7.67 TTQEE542 pKa = 4.19 DD543 pKa = 4.24 VSHH546 pKa = 7.57 IEE548 pKa = 4.18 VNGKK552 pKa = 8.74 RR553 pKa = 11.84 QMRR556 pKa = 11.84 GIVISSADD564 pKa = 3.54 VKK566 pKa = 11.45 GMDD569 pKa = 3.25 THH571 pKa = 7.62 IQINAAMNQHH581 pKa = 6.49 LGAIEE586 pKa = 3.99 ILDD589 pKa = 4.67 GIQYY593 pKa = 10.71 DD594 pKa = 3.66 VGPFRR599 pKa = 11.84 QTRR602 pKa = 11.84 AVIQDD607 pKa = 3.16 IQGNVYY613 pKa = 9.73 EE614 pKa = 4.6 RR615 pKa = 11.84 NLNGGQQAIAFGLANFSQTTGINSKK640 pKa = 10.75 YY641 pKa = 10.2 FGQIPNQEE649 pKa = 3.93 GTFPSGLITTSNHH662 pKa = 4.48 HH663 pKa = 5.42 TQMLTLLIEE672 pKa = 4.28 TAATTFTNEE681 pKa = 3.69 FGKK684 pKa = 10.76 SIAIAHH690 pKa = 6.11 LMILGDD696 pKa = 4.28 DD697 pKa = 3.94 VSLMLHH703 pKa = 6.6 GNDD706 pKa = 3.21 KK707 pKa = 10.97 DD708 pKa = 4.05 VNFFMKK714 pKa = 10.69 YY715 pKa = 9.71 LVEE718 pKa = 4.73 KK719 pKa = 10.33 FSQLGLILEE728 pKa = 4.58 RR729 pKa = 11.84 DD730 pKa = 3.41 EE731 pKa = 4.28 SRR733 pKa = 11.84 NFGVFLQQHH742 pKa = 6.09 VINGRR747 pKa = 11.84 FNGFSNRR754 pKa = 11.84 IAIFTSEE761 pKa = 4.16 DD762 pKa = 3.54 YY763 pKa = 8.9 KK764 pKa = 10.74 TRR766 pKa = 11.84 KK767 pKa = 9.14 SVQEE771 pKa = 3.77 SCTEE775 pKa = 3.88 YY776 pKa = 10.94 NALIDD781 pKa = 4.13 DD782 pKa = 4.62 VIFRR786 pKa = 11.84 TYY788 pKa = 10.56 NVRR791 pKa = 11.84 KK792 pKa = 9.4 LLQFQRR798 pKa = 11.84 IHH800 pKa = 5.82 QFVVLSKK807 pKa = 11.09 YY808 pKa = 9.85 IFRR811 pKa = 11.84 VQNYY815 pKa = 9.39 KK816 pKa = 10.14 YY817 pKa = 9.94 EE818 pKa = 4.04 KK819 pKa = 10.46 LKK821 pKa = 11.23 AKK823 pKa = 10.25 LATRR827 pKa = 11.84 LNVFEE832 pKa = 5.01 YY833 pKa = 10.71 DD834 pKa = 4.06 LKK836 pKa = 10.49 PHH838 pKa = 6.44 NDD840 pKa = 2.87 SRR842 pKa = 11.84 EE843 pKa = 3.99 NVQNQNMLRR852 pKa = 11.84 FIGIQIPYY860 pKa = 9.52 TYY862 pKa = 9.19 FQCSGGGEE870 pKa = 3.97 IPPEE874 pKa = 3.84 SFQRR878 pKa = 11.84 KK879 pKa = 9.22 DD880 pKa = 3.08 GSFTYY885 pKa = 9.68 EE886 pKa = 3.62 YY887 pKa = 10.62 SIYY890 pKa = 10.25 SPKK893 pKa = 10.71 GKK895 pKa = 9.17 WLRR898 pKa = 11.84 KK899 pKa = 8.5 FLYY902 pKa = 10.39 DD903 pKa = 3.54 ISCTIDD909 pKa = 3.29 EE910 pKa = 4.59 PKK912 pKa = 10.53 FRR914 pKa = 11.84 IDD916 pKa = 3.55 DD917 pKa = 4.48 EE918 pKa = 4.45 IMRR921 pKa = 11.84 MYY923 pKa = 11.08 NLDD926 pKa = 3.15 ICDD929 pKa = 4.97 FLIQYY934 pKa = 10.79 NMLNIQEE941 pKa = 4.7 EE942 pKa = 4.17 IRR944 pKa = 11.84 ATIIDD949 pKa = 3.85 RR950 pKa = 11.84 EE951 pKa = 4.56 LISKK955 pKa = 10.17 LAMNLEE961 pKa = 4.22 SLEE964 pKa = 4.03 NSNARR969 pKa = 11.84 MISRR973 pKa = 11.84 RR974 pKa = 11.84 ASEE977 pKa = 4.22 SLRR980 pKa = 11.84 IMGIKK985 pKa = 10.23 LPANGVYY992 pKa = 10.3 GYY994 pKa = 10.23 QINEE998 pKa = 4.07 RR999 pKa = 11.84 LVKK1002 pKa = 10.27 VLQNIQQSDD1011 pKa = 4.59 YY1012 pKa = 8.88 EE1013 pKa = 4.46 VKK1015 pKa = 10.17 MVGDD1019 pKa = 4.12 ALFTAIMEE1027 pKa = 4.61 KK1028 pKa = 10.29 FDD1030 pKa = 3.48 SHH1032 pKa = 6.18 KK1033 pKa = 10.92 VRR1035 pKa = 11.84 MEE1037 pKa = 3.84 KK1038 pKa = 10.56 GDD1040 pKa = 3.64 RR1041 pKa = 11.84 LHH1043 pKa = 6.96 NFTLDD1048 pKa = 3.52 FSDD1051 pKa = 3.62 KK1052 pKa = 10.75 SDD1054 pKa = 3.2 RR1055 pKa = 11.84 LVINKK1060 pKa = 9.43 KK1061 pKa = 9.73 MIALHH1066 pKa = 6.32 NISISKK1072 pKa = 10.29 NMAPYY1077 pKa = 10.27 SDD1079 pKa = 4.19 AWMLYY1084 pKa = 7.86 TCLNHH1089 pKa = 6.78 TYY1091 pKa = 9.97 NASSEE1096 pKa = 4.15 LSTALAHH1103 pKa = 5.96 SQGHH1107 pKa = 5.61 FKK1109 pKa = 10.8 SFQYY1113 pKa = 10.75 DD1114 pKa = 2.77 RR1115 pKa = 11.84 DD1116 pKa = 3.68 MFAEE1120 pKa = 4.34 SVKK1123 pKa = 10.22 IASKK1127 pKa = 10.53 HH1128 pKa = 5.77 GIGSLPMEE1136 pKa = 4.84 LFFEE1140 pKa = 4.74 ASNIRR1145 pKa = 11.84 DD1146 pKa = 3.59 SAQLKK1151 pKa = 8.52 WIEE1154 pKa = 4.16 AIKK1157 pKa = 10.74 YY1158 pKa = 7.23 YY1159 pKa = 10.24 IQFKK1163 pKa = 10.53 DD1164 pKa = 3.28 YY1165 pKa = 10.75 LYY1167 pKa = 9.8 PYY1169 pKa = 9.42 SINPRR1174 pKa = 11.84 RR1175 pKa = 11.84 LFFIPEE1181 pKa = 4.09 QVSSVSSLLNQDD1193 pKa = 5.03 NIPADD1198 pKa = 3.89 ANKK1201 pKa = 9.46 KK1202 pKa = 9.98 ALMFRR1207 pKa = 11.84 RR1208 pKa = 11.84 AYY1210 pKa = 10.35 AYY1212 pKa = 10.39 VLSHH1216 pKa = 7.2 PFRR1219 pKa = 11.84 ISGAKK1224 pKa = 10.13 AIFVDD1229 pKa = 5.12 DD1230 pKa = 5.1 RR1231 pKa = 11.84 ISS1233 pKa = 2.94
Molecular weight: 143.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.53
IPC2_protein 7.892
IPC_protein 7.732
Toseland 7.761
ProMoST 8.258
Dawson 8.521
Bjellqvist 8.624
Wikipedia 8.565
Rodwell 8.58
Grimsley 7.892
Solomon 8.58
Lehninger 8.551
Nozaki 8.639
DTASelect 8.478
Thurlkill 8.536
EMBOSS 8.682
Sillero 8.756
Patrickios 4.228
IPC_peptide 8.565
IPC2_peptide 7.395
IPC2.peptide.svr19 7.72
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
7194
278
1233
799.3
91.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.698 ± 0.338
1.251 ± 0.289
5.977 ± 0.185
5.518 ± 0.221
4.712 ± 0.372
3.92 ± 0.199
2.294 ± 0.327
9.077 ± 0.377
5.699 ± 0.249
9.258 ± 0.291
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.863 ± 0.207
6.909 ± 0.237
3.322 ± 0.308
4.712 ± 0.147
4.504 ± 0.324
7.353 ± 0.25
6.756 ± 0.483
6.005 ± 0.347
0.639 ± 0.082
4.532 ± 0.345
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here