Stenotrophomonas phage SMA6

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Scuticavirus; Stenotrophomonas virus SMA6

Average proteome isoelectric point is 7.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S0F2M9|S0F2M9_9VIRU DNA-Binding protein G5P OS=Stenotrophomonas phage SMA6 OX=1346893 PE=3 SV=1
MM1 pKa = 7.49AVLIPACRR9 pKa = 11.84EE10 pKa = 3.76ADD12 pKa = 3.65LDD14 pKa = 4.2TAAGTCTAVIWIPQPALLPEE34 pKa = 4.54LPIEE38 pKa = 4.4DD39 pKa = 3.96AQAIGAKK46 pKa = 9.34IALLWAVAYY55 pKa = 8.25VFRR58 pKa = 11.84LIRR61 pKa = 11.84KK62 pKa = 9.49KK63 pKa = 10.32IEE65 pKa = 3.68QSS67 pKa = 3.03

Molecular weight: 7.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0F2N7|S0F2N7_9VIRU TrbP-related OS=Stenotrophomonas phage SMA6 OX=1346893 PE=4 SV=1
MM1 pKa = 8.27RR2 pKa = 11.84DD3 pKa = 3.23RR4 pKa = 11.84KK5 pKa = 9.38LTGPWAGFSFKK16 pKa = 10.38GGRR19 pKa = 11.84LVTPEE24 pKa = 3.7GRR26 pKa = 11.84EE27 pKa = 4.6LEE29 pKa = 4.44PQDD32 pKa = 5.12LAWLSLTAAQAQEE45 pKa = 3.98WRR47 pKa = 11.84RR48 pKa = 11.84MMEE51 pKa = 3.64SSRR54 pKa = 11.84AIDD57 pKa = 3.49KK58 pKa = 9.97PRR60 pKa = 11.84NPLSFNAASVVNLSDD75 pKa = 4.63ALAQRR80 pKa = 11.84RR81 pKa = 11.84KK82 pKa = 9.78KK83 pKa = 10.42RR84 pKa = 11.84SPGAMAGPDD93 pKa = 4.15AEE95 pKa = 4.71PPAAVLPVPGPKK107 pKa = 9.2RR108 pKa = 11.84RR109 pKa = 11.84QRR111 pKa = 11.84VV112 pKa = 3.04

Molecular weight: 12.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

2181

67

630

218.1

23.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.517 ± 1.284

2.843 ± 0.871

4.585 ± 0.446

4.814 ± 0.865

3.026 ± 0.587

10.271 ± 1.572

1.788 ± 0.315

3.576 ± 0.704

3.897 ± 0.375

8.666 ± 1.325

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.155 ± 0.332

2.43 ± 0.529

6.052 ± 0.795

3.897 ± 0.495

7.84 ± 0.666

5.731 ± 0.593

4.448 ± 0.616

6.694 ± 0.829

2.522 ± 0.307

2.247 ± 0.416
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski