Amino acid dipepetide frequency for Stenotrophomonas phage SMA6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.89AlaAla: 17.89 ± 4.69
3.211AlaCys: 3.211 ± 1.283
3.211AlaAsp: 3.211 ± 1.052
7.339AlaGlu: 7.339 ± 2.099
4.587AlaPhe: 4.587 ± 3.559
10.092AlaGly: 10.092 ± 1.493
1.835AlaHis: 1.835 ± 0.536
6.422AlaIle: 6.422 ± 2.493
7.798AlaLys: 7.798 ± 1.606
11.927AlaLeu: 11.927 ± 2.49
4.128AlaMet: 4.128 ± 1.621
0.0AlaAsn: 0.0 ± 0.0
6.881AlaPro: 6.881 ± 1.883
7.798AlaGln: 7.798 ± 1.789
7.339AlaArg: 7.339 ± 1.732
5.963AlaSer: 5.963 ± 1.729
3.211AlaThr: 3.211 ± 1.239
9.174AlaVal: 9.174 ± 2.565
2.752AlaTrp: 2.752 ± 1.095
3.67AlaTyr: 3.67 ± 1.654
0.0AlaXaa: 0.0 ± 0.0
Cys
4.587CysAla: 4.587 ± 1.746
0.917CysCys: 0.917 ± 0.681
1.376CysAsp: 1.376 ± 1.021
1.835CysGlu: 1.835 ± 0.743
0.459CysPhe: 0.459 ± 0.34
0.917CysGly: 0.917 ± 0.681
0.0CysHis: 0.0 ± 0.0
0.0CysIle: 0.0 ± 0.0
1.835CysLys: 1.835 ± 0.9
2.752CysLeu: 2.752 ± 0.667
0.459CysMet: 0.459 ± 0.364
0.917CysAsn: 0.917 ± 0.681
2.752CysPro: 2.752 ± 1.344
0.917CysGln: 0.917 ± 0.517
3.211CysArg: 3.211 ± 1.073
1.835CysSer: 1.835 ± 0.966
2.752CysThr: 2.752 ± 1.022
0.917CysVal: 0.917 ± 0.45
0.0CysTrp: 0.0 ± 0.0
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
7.798AspAla: 7.798 ± 1.706
1.376AspCys: 1.376 ± 0.672
1.835AspAsp: 1.835 ± 0.627
2.294AspGlu: 2.294 ± 0.777
0.917AspPhe: 0.917 ± 0.499
5.963AspGly: 5.963 ± 2.348
1.376AspHis: 1.376 ± 0.544
0.459AspIle: 0.459 ± 0.571
0.917AspLys: 0.917 ± 0.9
4.587AspLeu: 4.587 ± 2.167
0.459AspMet: 0.459 ± 0.364
1.376AspAsn: 1.376 ± 1.021
2.752AspPro: 2.752 ± 1.097
1.376AspGln: 1.376 ± 0.803
3.67AspArg: 3.67 ± 1.369
1.835AspSer: 1.835 ± 0.521
1.376AspThr: 1.376 ± 0.813
3.67AspVal: 3.67 ± 0.819
0.459AspTrp: 0.459 ± 0.43
1.376AspTyr: 1.376 ± 0.972
0.0AspXaa: 0.0 ± 0.0
Glu
5.046GluAla: 5.046 ± 2.344
1.376GluCys: 1.376 ± 0.688
2.294GluAsp: 2.294 ± 1.09
2.752GluGlu: 2.752 ± 1.092
0.917GluPhe: 0.917 ± 1.005
6.422GluGly: 6.422 ± 1.832
0.459GluHis: 0.459 ± 0.364
1.835GluIle: 1.835 ± 0.908
3.67GluLys: 3.67 ± 1.506
4.587GluLeu: 4.587 ± 1.867
0.459GluMet: 0.459 ± 0.592
0.459GluAsn: 0.459 ± 0.43
2.294GluPro: 2.294 ± 1.385
2.752GluGln: 2.752 ± 0.905
4.587GluArg: 4.587 ± 1.992
1.376GluSer: 1.376 ± 0.906
0.917GluThr: 0.917 ± 0.613
4.587GluVal: 4.587 ± 2.322
0.459GluTrp: 0.459 ± 0.614
0.917GluTyr: 0.917 ± 0.565
0.0GluXaa: 0.0 ± 0.0
Phe
5.046PheAla: 5.046 ± 1.425
0.459PheCys: 0.459 ± 0.43
0.917PheAsp: 0.917 ± 0.626
0.917PheGlu: 0.917 ± 0.565
0.0PhePhe: 0.0 ± 0.0
4.587PheGly: 4.587 ± 1.1
0.0PheHis: 0.0 ± 0.0
1.376PheIle: 1.376 ± 0.585
0.917PheLys: 0.917 ± 0.675
1.376PheLeu: 1.376 ± 1.133
0.0PheMet: 0.0 ± 0.0
0.917PheAsn: 0.917 ± 0.825
0.917PhePro: 0.917 ± 0.59
0.459PheGln: 0.459 ± 0.561
4.128PheArg: 4.128 ± 1.973
1.835PheSer: 1.835 ± 0.7
1.376PheThr: 1.376 ± 0.453
3.67PheVal: 3.67 ± 1.348
0.0PheTrp: 0.0 ± 0.0
1.376PheTyr: 1.376 ± 0.572
0.0PheXaa: 0.0 ± 0.0
Gly
9.633GlyAla: 9.633 ± 1.596
3.67GlyCys: 3.67 ± 1.757
4.128GlyAsp: 4.128 ± 1.409
5.046GlyGlu: 5.046 ± 1.147
3.211GlyPhe: 3.211 ± 1.34
12.385GlyGly: 12.385 ± 3.944
2.294GlyHis: 2.294 ± 1.162
1.835GlyIle: 1.835 ± 0.77
4.128GlyLys: 4.128 ± 1.491
9.633GlyLeu: 9.633 ± 1.709
0.917GlyMet: 0.917 ± 0.562
5.046GlyAsn: 5.046 ± 2.0
5.046GlyPro: 5.046 ± 1.595
6.881GlyGln: 6.881 ± 2.513
7.798GlyArg: 7.798 ± 2.915
6.422GlySer: 6.422 ± 1.42
5.505GlyThr: 5.505 ± 2.059
3.211GlyVal: 3.211 ± 1.319
2.294GlyTrp: 2.294 ± 1.047
3.211GlyTyr: 3.211 ± 1.703
0.0GlyXaa: 0.0 ± 0.0
His
2.752HisAla: 2.752 ± 0.559
0.0HisCys: 0.0 ± 0.0
0.917HisAsp: 0.917 ± 0.499
0.0HisGlu: 0.0 ± 0.0
0.0HisPhe: 0.0 ± 0.0
0.917HisGly: 0.917 ± 0.876
0.0HisHis: 0.0 ± 0.0
0.0HisIle: 0.0 ± 0.0
0.459HisLys: 0.459 ± 0.43
2.752HisLeu: 2.752 ± 0.958
0.459HisMet: 0.459 ± 0.43
0.917HisAsn: 0.917 ± 0.709
0.459HisPro: 0.459 ± 0.43
0.917HisGln: 0.917 ± 0.681
2.752HisArg: 2.752 ± 0.906
0.459HisSer: 0.459 ± 0.592
0.917HisThr: 0.917 ± 0.59
2.752HisVal: 2.752 ± 1.001
0.459HisTrp: 0.459 ± 0.364
0.0HisTyr: 0.0 ± 0.0
0.0HisXaa: 0.0 ± 0.0
Ile
4.587IleAla: 4.587 ± 1.19
0.917IleCys: 0.917 ± 0.565
4.587IleAsp: 4.587 ± 1.642
1.835IleGlu: 1.835 ± 1.636
1.376IlePhe: 1.376 ± 0.76
2.752IleGly: 2.752 ± 1.096
0.0IleHis: 0.0 ± 0.0
1.376IleIle: 1.376 ± 0.627
1.376IleLys: 1.376 ± 0.76
1.835IleLeu: 1.835 ± 1.064
0.459IleMet: 0.459 ± 0.34
0.917IleAsn: 0.917 ± 0.565
2.294IlePro: 2.294 ± 1.654
0.917IleGln: 0.917 ± 0.681
3.211IleArg: 3.211 ± 1.344
1.376IleSer: 1.376 ± 0.664
0.917IleThr: 0.917 ± 0.684
3.67IleVal: 3.67 ± 1.598
0.917IleTrp: 0.917 ± 0.702
0.459IleTyr: 0.459 ± 0.508
0.0IleXaa: 0.0 ± 0.0
Lys
3.67LysAla: 3.67 ± 1.062
2.294LysCys: 2.294 ± 1.356
1.835LysAsp: 1.835 ± 0.866
1.376LysGlu: 1.376 ± 0.76
0.917LysPhe: 0.917 ± 1.005
4.128LysGly: 4.128 ± 2.373
1.376LysHis: 1.376 ± 0.999
1.376LysIle: 1.376 ± 1.545
4.128LysLys: 4.128 ± 1.394
2.294LysLeu: 2.294 ± 1.059
0.917LysMet: 0.917 ± 0.433
1.376LysAsn: 1.376 ± 0.672
1.376LysPro: 1.376 ± 0.864
0.917LysGln: 0.917 ± 0.45
2.294LysArg: 2.294 ± 1.599
3.211LysSer: 3.211 ± 0.6
2.294LysThr: 2.294 ± 0.863
1.376LysVal: 1.376 ± 0.544
1.835LysTrp: 1.835 ± 0.818
0.0LysTyr: 0.0 ± 0.0
0.0LysXaa: 0.0 ± 0.0
Leu
11.468LeuAla: 11.468 ± 3.841
1.835LeuCys: 1.835 ± 0.87
6.881LeuAsp: 6.881 ± 2.588
2.752LeuGlu: 2.752 ± 0.994
1.835LeuPhe: 1.835 ± 0.697
5.046LeuGly: 5.046 ± 1.77
0.917LeuHis: 0.917 ± 0.499
3.67LeuIle: 3.67 ± 1.644
1.376LeuLys: 1.376 ± 0.721
10.55LeuLeu: 10.55 ± 2.743
2.752LeuMet: 2.752 ± 0.945
1.835LeuAsn: 1.835 ± 0.536
6.422LeuPro: 6.422 ± 1.9
2.294LeuGln: 2.294 ± 0.741
5.046LeuArg: 5.046 ± 1.446
6.422LeuSer: 6.422 ± 2.302
5.046LeuThr: 5.046 ± 1.379
6.881LeuVal: 6.881 ± 2.311
2.294LeuTrp: 2.294 ± 1.418
1.376LeuTyr: 1.376 ± 0.857
0.0LeuXaa: 0.0 ± 0.0
Met
4.128MetAla: 4.128 ± 0.862
0.459MetCys: 0.459 ± 0.34
0.0MetAsp: 0.0 ± 0.0
3.211MetGlu: 3.211 ± 0.907
0.0MetPhe: 0.0 ± 0.0
1.376MetGly: 1.376 ± 0.453
0.917MetHis: 0.917 ± 0.613
0.917MetIle: 0.917 ± 0.739
0.459MetLys: 0.459 ± 0.503
2.294MetLeu: 2.294 ± 0.938
0.917MetMet: 0.917 ± 0.675
0.0MetAsn: 0.0 ± 0.0
2.294MetPro: 2.294 ± 0.934
0.459MetGln: 0.459 ± 0.34
1.835MetArg: 1.835 ± 1.021
0.459MetSer: 0.459 ± 0.364
0.917MetThr: 0.917 ± 0.59
0.917MetVal: 0.917 ± 0.609
0.0MetTrp: 0.0 ± 0.0
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
0.917AsnAla: 0.917 ± 0.622
0.459AsnCys: 0.459 ± 0.43
0.459AsnAsp: 0.459 ± 0.34
2.294AsnGlu: 2.294 ± 0.694
0.459AsnPhe: 0.459 ± 0.43
5.046AsnGly: 5.046 ± 2.0
0.0AsnHis: 0.0 ± 0.0
0.917AsnIle: 0.917 ± 0.517
0.917AsnLys: 0.917 ± 0.728
1.376AsnLeu: 1.376 ± 1.226
0.0AsnMet: 0.0 ± 0.0
0.459AsnAsn: 0.459 ± 0.364
1.835AsnPro: 1.835 ± 0.543
0.459AsnGln: 0.459 ± 0.364
0.917AsnArg: 0.917 ± 0.565
1.835AsnSer: 1.835 ± 1.0
2.752AsnThr: 2.752 ± 1.064
1.376AsnVal: 1.376 ± 1.021
1.376AsnTrp: 1.376 ± 0.572
0.459AsnTyr: 0.459 ± 0.364
0.0AsnXaa: 0.0 ± 0.0
Pro
5.505ProAla: 5.505 ± 1.674
0.459ProCys: 0.459 ± 0.34
3.211ProAsp: 3.211 ± 1.588
3.67ProGlu: 3.67 ± 1.264
2.294ProPhe: 2.294 ± 1.241
8.716ProGly: 8.716 ± 1.09
0.459ProHis: 0.459 ± 0.592
3.211ProIle: 3.211 ± 0.995
0.459ProLys: 0.459 ± 0.614
5.046ProLeu: 5.046 ± 1.76
1.376ProMet: 1.376 ± 0.827
1.835ProAsn: 1.835 ± 0.866
4.128ProPro: 4.128 ± 1.766
2.294ProGln: 2.294 ± 1.135
5.046ProArg: 5.046 ± 1.053
5.505ProSer: 5.505 ± 1.503
0.459ProThr: 0.459 ± 0.364
3.211ProVal: 3.211 ± 1.121
2.752ProTrp: 2.752 ± 1.221
0.917ProTyr: 0.917 ± 0.595
0.0ProXaa: 0.0 ± 0.0
Gln
4.128GlnAla: 4.128 ± 1.476
1.835GlnCys: 1.835 ± 0.966
2.294GlnAsp: 2.294 ± 0.849
1.376GlnGlu: 1.376 ± 0.906
0.0GlnPhe: 0.0 ± 0.0
5.505GlnGly: 5.505 ± 2.352
0.459GlnHis: 0.459 ± 0.364
0.459GlnIle: 0.459 ± 0.34
0.459GlnLys: 0.459 ± 0.43
1.835GlnLeu: 1.835 ± 0.827
0.459GlnMet: 0.459 ± 0.503
0.0GlnAsn: 0.0 ± 0.0
4.587GlnPro: 4.587 ± 1.259
2.294GlnGln: 2.294 ± 0.777
4.587GlnArg: 4.587 ± 1.083
2.752GlnSer: 2.752 ± 0.863
1.376GlnThr: 1.376 ± 0.534
1.835GlnVal: 1.835 ± 0.627
1.835GlnTrp: 1.835 ± 0.991
0.917GlnTyr: 0.917 ± 0.595
0.0GlnXaa: 0.0 ± 0.0
Arg
5.963ArgAla: 5.963 ± 1.803
0.917ArgCys: 0.917 ± 0.433
3.67ArgAsp: 3.67 ± 1.097
4.128ArgGlu: 4.128 ± 1.919
2.294ArgPhe: 2.294 ± 1.679
7.339ArgGly: 7.339 ± 1.861
4.128ArgHis: 4.128 ± 0.895
4.128ArgIle: 4.128 ± 0.994
3.211ArgLys: 3.211 ± 1.338
5.505ArgLeu: 5.505 ± 1.607
1.376ArgMet: 1.376 ± 0.589
2.294ArgAsn: 2.294 ± 0.797
5.505ArgPro: 5.505 ± 1.45
2.294ArgGln: 2.294 ± 1.122
5.505ArgArg: 5.505 ± 2.121
7.798ArgSer: 7.798 ± 2.05
3.211ArgThr: 3.211 ± 0.886
6.422ArgVal: 6.422 ± 2.148
2.294ArgTrp: 2.294 ± 0.715
0.459ArgTyr: 0.459 ± 0.571
0.0ArgXaa: 0.0 ± 0.0
Ser
10.092SerAla: 10.092 ± 1.847
1.376SerCys: 1.376 ± 0.688
4.128SerAsp: 4.128 ± 1.026
1.376SerGlu: 1.376 ± 0.544
3.67SerPhe: 3.67 ± 1.392
3.67SerGly: 3.67 ± 2.322
0.917SerHis: 0.917 ± 0.59
0.917SerIle: 0.917 ± 0.876
2.752SerLys: 2.752 ± 1.231
4.587SerLeu: 4.587 ± 1.458
1.835SerMet: 1.835 ± 0.711
0.917SerAsn: 0.917 ± 0.45
3.67SerPro: 3.67 ± 0.882
2.294SerGln: 2.294 ± 1.335
3.67SerArg: 3.67 ± 0.824
3.211SerSer: 3.211 ± 0.68
1.835SerThr: 1.835 ± 0.881
2.752SerVal: 2.752 ± 1.677
1.376SerTrp: 1.376 ± 0.798
2.294SerTyr: 2.294 ± 0.774
0.0SerXaa: 0.0 ± 0.0
Thr
7.798ThrAla: 7.798 ± 2.281
3.67ThrCys: 3.67 ± 2.232
0.459ThrAsp: 0.459 ± 0.34
1.835ThrGlu: 1.835 ± 0.521
1.835ThrPhe: 1.835 ± 1.025
6.881ThrGly: 6.881 ± 1.672
0.459ThrHis: 0.459 ± 0.34
1.835ThrIle: 1.835 ± 0.97
1.376ThrLys: 1.376 ± 0.628
1.835ThrLeu: 1.835 ± 0.697
0.917ThrMet: 0.917 ± 0.626
1.376ThrAsn: 1.376 ± 0.813
3.67ThrPro: 3.67 ± 1.237
1.835ThrGln: 1.835 ± 1.061
2.294ThrArg: 2.294 ± 0.89
0.917ThrSer: 0.917 ± 0.728
2.294ThrThr: 2.294 ± 1.412
1.835ThrVal: 1.835 ± 0.543
0.459ThrTrp: 0.459 ± 0.34
0.917ThrTyr: 0.917 ± 0.681
0.0ThrXaa: 0.0 ± 0.0
Val
9.633ValAla: 9.633 ± 2.659
2.294ValCys: 2.294 ± 0.805
2.752ValAsp: 2.752 ± 0.834
2.294ValGlu: 2.294 ± 0.821
3.67ValPhe: 3.67 ± 1.203
5.505ValGly: 5.505 ± 1.751
1.835ValHis: 1.835 ± 0.536
2.294ValIle: 2.294 ± 1.028
1.376ValLys: 1.376 ± 0.737
7.798ValLeu: 7.798 ± 2.32
2.294ValMet: 2.294 ± 0.859
1.376ValAsn: 1.376 ± 0.589
2.752ValPro: 2.752 ± 1.316
0.917ValGln: 0.917 ± 0.563
6.422ValArg: 6.422 ± 1.371
2.294ValSer: 2.294 ± 1.227
2.752ValThr: 2.752 ± 1.214
4.128ValVal: 4.128 ± 1.387
1.835ValTrp: 1.835 ± 0.774
2.752ValTyr: 2.752 ± 1.024
0.0ValXaa: 0.0 ± 0.0
Trp
3.211TrpAla: 3.211 ± 1.301
0.917TrpCys: 0.917 ± 0.533
0.459TrpAsp: 0.459 ± 0.34
0.0TrpGlu: 0.0 ± 0.0
0.917TrpPhe: 0.917 ± 0.433
2.752TrpGly: 2.752 ± 0.92
0.459TrpHis: 0.459 ± 0.592
2.294TrpIle: 2.294 ± 0.891
0.459TrpLys: 0.459 ± 0.364
2.752TrpLeu: 2.752 ± 1.266
0.459TrpMet: 0.459 ± 0.43
1.376TrpAsn: 1.376 ± 0.453
0.459TrpPro: 0.459 ± 0.364
0.459TrpGln: 0.459 ± 0.34
2.752TrpArg: 2.752 ± 1.457
0.917TrpSer: 0.917 ± 0.645
2.294TrpThr: 2.294 ± 0.915
1.376TrpVal: 1.376 ± 0.58
0.917TrpTrp: 0.917 ± 0.681
0.0TrpTyr: 0.0 ± 0.0
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.376TyrAla: 1.376 ± 0.68
0.0TyrCys: 0.0 ± 0.0
0.459TyrAsp: 0.459 ± 0.571
1.835TyrGlu: 1.835 ± 1.262
0.917TyrPhe: 0.917 ± 0.681
2.752TyrGly: 2.752 ± 1.004
0.0TyrHis: 0.0 ± 0.0
0.459TyrIle: 0.459 ± 0.364
0.917TyrLys: 0.917 ± 0.499
1.376TyrLeu: 1.376 ± 0.857
0.459TyrMet: 0.459 ± 0.364
0.917TyrAsn: 0.917 ± 0.59
0.459TyrPro: 0.459 ± 0.364
0.459TyrGln: 0.459 ± 0.508
1.376TyrArg: 1.376 ± 1.092
0.917TyrSer: 0.917 ± 0.45
2.294TyrThr: 2.294 ± 0.919
3.211TyrVal: 3.211 ± 1.422
0.917TyrTrp: 0.917 ± 0.517
1.376TyrTyr: 1.376 ± 0.704
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 10 proteins (2181 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski