Streptomyces laurentii

Taxonomy: cellular organisms; Bacteria;

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7440 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160NW43|A0A160NW43_STRLU Alkyl hydroperoxide reductase OS=Streptomyces laurentii OX=39478 GN=SLA_1990 PE=4 SV=1
MM1 pKa = 8.08DD2 pKa = 5.69APTDD6 pKa = 3.76SDD8 pKa = 3.82VSGDD12 pKa = 3.59ARR14 pKa = 11.84VEE16 pKa = 3.83ALSPDD21 pKa = 3.51RR22 pKa = 11.84TVVYY26 pKa = 10.42YY27 pKa = 10.72DD28 pKa = 3.51AADD31 pKa = 3.87EE32 pKa = 4.37VLHH35 pKa = 5.92PTLVLGFGEE44 pKa = 4.19LCIVKK49 pKa = 9.98VSNDD53 pKa = 3.48DD54 pKa = 3.16DD55 pKa = 3.95WYY57 pKa = 10.1MGSRR61 pKa = 11.84NDD63 pKa = 5.1DD64 pKa = 3.05GSAICWSIYY73 pKa = 10.21ADD75 pKa = 3.7LYY77 pKa = 10.0EE78 pKa = 4.61ALRR81 pKa = 11.84GLL83 pKa = 4.04

Molecular weight: 9.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A169NSJ5|A0A169NSJ5_STRLU Cold shock protein scoF OS=Streptomyces laurentii OX=39478 GN=SLA_4364 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.46GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7440

0

7440

2333776

39

6066

313.7

33.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.104 ± 0.05

0.789 ± 0.008

5.98 ± 0.024

5.607 ± 0.03

2.648 ± 0.016

9.694 ± 0.028

2.236 ± 0.013

2.981 ± 0.02

2.089 ± 0.026

10.207 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.71 ± 0.012

1.651 ± 0.016

6.48 ± 0.029

2.55 ± 0.016

8.276 ± 0.033

4.854 ± 0.022

6.306 ± 0.033

8.311 ± 0.026

1.493 ± 0.013

2.034 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski