Amino acid dipepetide frequency for Streptomyces laurentii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.891AlaAla: 22.891 ± 0.178	1.08AlaCys: 1.08 ± 0.024	8.732AlaAsp: 8.732 ± 0.065	9.068AlaGlu: 9.068 ± 0.094	3.524AlaPhe: 3.524 ± 0.039	14.323AlaGly: 14.323 ± 0.097	2.954AlaHis: 2.954 ± 0.039	3.28AlaIle: 3.28 ± 0.042	2.796AlaLys: 2.796 ± 0.046	14.845AlaLeu: 14.845 ± 0.107	2.533AlaMet: 2.533 ± 0.032	1.789AlaAsn: 1.789 ± 0.031	8.174AlaPro: 8.174 ± 0.093	3.5AlaGln: 3.5 ± 0.049	11.171AlaArg: 11.171 ± 0.088	5.848AlaSer: 5.848 ± 0.052	7.351AlaThr: 7.351 ± 0.064	12.286AlaVal: 12.286 ± 0.105	1.933AlaTrp: 1.933 ± 0.034	2.964AlaTyr: 2.964 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	1.164CysAla: 1.164 ± 0.024	0.102CysCys: 0.102 ± 0.007	0.449CysAsp: 0.449 ± 0.015	0.368CysGlu: 0.368 ± 0.014	0.225CysPhe: 0.225 ± 0.009	0.993CysGly: 0.993 ± 0.024	0.196CysHis: 0.196 ± 0.009	0.129CysIle: 0.129 ± 0.008	0.106CysLys: 0.106 ± 0.007	0.743CysLeu: 0.743 ± 0.019	0.131CysMet: 0.131 ± 0.008	0.12CysAsn: 0.12 ± 0.008	0.551CysPro: 0.551 ± 0.017	0.155CysGln: 0.155 ± 0.007	0.621CysArg: 0.621 ± 0.018	0.407CysSer: 0.407 ± 0.014	0.465CysThr: 0.465 ± 0.013	0.695CysVal: 0.695 ± 0.017	0.125CysTrp: 0.125 ± 0.007	0.142CysTyr: 0.142 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	7.978AspAla: 7.978 ± 0.068	0.431AspCys: 0.431 ± 0.015	3.508AspAsp: 3.508 ± 0.046	3.884AspGlu: 3.884 ± 0.05	1.585AspPhe: 1.585 ± 0.029	6.615AspGly: 6.615 ± 0.065	1.408AspHis: 1.408 ± 0.027	1.82AspIle: 1.82 ± 0.032	1.182AspLys: 1.182 ± 0.027	6.1AspLeu: 6.1 ± 0.052	0.786AspMet: 0.786 ± 0.019	0.875AspAsn: 0.875 ± 0.021	4.719AspPro: 4.719 ± 0.052	1.516AspGln: 1.516 ± 0.03	5.089AspArg: 5.089 ± 0.06	2.379AspSer: 2.379 ± 0.028	3.481AspThr: 3.481 ± 0.039	4.4AspVal: 4.4 ± 0.049	1.004AspTrp: 1.004 ± 0.022	1.036AspTyr: 1.036 ± 0.025	0.0AspXaa: 0.0 ± 0.0
Glu	7.568GluAla: 7.568 ± 0.081	0.351GluCys: 0.351 ± 0.013	2.834GluAsp: 2.834 ± 0.034	3.479GluGlu: 3.479 ± 0.055	1.425GluPhe: 1.425 ± 0.023	4.31GluGly: 4.31 ± 0.048	1.403GluHis: 1.403 ± 0.025	2.226GluIle: 2.226 ± 0.034	1.38GluLys: 1.38 ± 0.034	6.492GluLeu: 6.492 ± 0.059	0.859GluMet: 0.859 ± 0.019	0.986GluAsn: 0.986 ± 0.021	3.523GluPro: 3.523 ± 0.055	2.011GluGln: 2.011 ± 0.035	5.662GluArg: 5.662 ± 0.062	2.523GluSer: 2.523 ± 0.036	3.056GluThr: 3.056 ± 0.038	4.189GluVal: 4.189 ± 0.051	0.744GluTrp: 0.744 ± 0.017	1.05GluTyr: 1.05 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	3.671PheAla: 3.671 ± 0.04	0.264PheCys: 0.264 ± 0.01	1.893PheAsp: 1.893 ± 0.03	1.386PheGlu: 1.386 ± 0.026	0.863PhePhe: 0.863 ± 0.024	2.96PheGly: 2.96 ± 0.037	0.584PheHis: 0.584 ± 0.014	0.626PheIle: 0.626 ± 0.018	0.478PheLys: 0.478 ± 0.014	2.586PheLeu: 2.586 ± 0.041	0.391PheMet: 0.391 ± 0.013	0.502PheAsn: 0.502 ± 0.017	1.393PhePro: 1.393 ± 0.026	0.663PheGln: 0.663 ± 0.017	1.847PheArg: 1.847 ± 0.031	1.373PheSer: 1.373 ± 0.025	1.957PheThr: 1.957 ± 0.027	2.13PheVal: 2.13 ± 0.034	0.396PheTrp: 0.396 ± 0.014	0.521PheTyr: 0.521 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	11.806GlyAla: 11.806 ± 0.09	0.885GlyCys: 0.885 ± 0.021	5.311GlyAsp: 5.311 ± 0.053	5.129GlyGlu: 5.129 ± 0.05	2.87GlyPhe: 2.87 ± 0.038	9.262GlyGly: 9.262 ± 0.099	2.348GlyHis: 2.348 ± 0.035	3.313GlyIle: 3.313 ± 0.036	2.354GlyLys: 2.354 ± 0.039	9.664GlyLeu: 9.664 ± 0.08	1.94GlyMet: 1.94 ± 0.035	1.709GlyAsn: 1.709 ± 0.035	5.864GlyPro: 5.864 ± 0.056	2.587GlyGln: 2.587 ± 0.034	8.205GlyArg: 8.205 ± 0.057	5.28GlySer: 5.28 ± 0.056	6.941GlyThr: 6.941 ± 0.069	7.466GlyVal: 7.466 ± 0.062	1.736GlyTrp: 1.736 ± 0.029	2.269GlyTyr: 2.269 ± 0.033	0.0GlyXaa: 0.0 ± 0.0
His	2.767HisAla: 2.767 ± 0.039	0.199HisCys: 0.199 ± 0.01	1.334HisAsp: 1.334 ± 0.024	1.203HisGlu: 1.203 ± 0.024	0.607HisPhe: 0.607 ± 0.019	2.396HisGly: 2.396 ± 0.032	0.678HisHis: 0.678 ± 0.02	0.609HisIle: 0.609 ± 0.016	0.337HisLys: 0.337 ± 0.013	2.299HisLeu: 2.299 ± 0.034	0.331HisMet: 0.331 ± 0.013	0.34HisAsn: 0.34 ± 0.013	1.869HisPro: 1.869 ± 0.032	0.625HisGln: 0.625 ± 0.016	2.052HisArg: 2.052 ± 0.032	0.935HisSer: 0.935 ± 0.022	1.308HisThr: 1.308 ± 0.022	1.661HisVal: 1.661 ± 0.029	0.344HisTrp: 0.344 ± 0.011	0.461HisTyr: 0.461 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	4.598IleAla: 4.598 ± 0.05	0.279IleCys: 0.279 ± 0.011	2.082IleAsp: 2.082 ± 0.035	1.854IleGlu: 1.854 ± 0.028	0.623IlePhe: 0.623 ± 0.016	3.36IleGly: 3.36 ± 0.045	0.588IleHis: 0.588 ± 0.015	0.747IleIle: 0.747 ± 0.021	0.665IleLys: 0.665 ± 0.018	2.367IleLeu: 2.367 ± 0.033	0.411IleMet: 0.411 ± 0.015	0.599IleAsn: 0.599 ± 0.02	1.731IlePro: 1.731 ± 0.028	0.689IleGln: 0.689 ± 0.018	2.258IleArg: 2.258 ± 0.03	1.429IleSer: 1.429 ± 0.026	2.084IleThr: 2.084 ± 0.03	2.635IleVal: 2.635 ± 0.04	0.35IleTrp: 0.35 ± 0.013	0.46IleTyr: 0.46 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	2.981LysAla: 2.981 ± 0.05	0.114LysCys: 0.114 ± 0.008	1.302LysAsp: 1.302 ± 0.026	1.157LysGlu: 1.157 ± 0.027	0.409LysPhe: 0.409 ± 0.015	1.78LysGly: 1.78 ± 0.034	0.405LysHis: 0.405 ± 0.014	0.852LysIle: 0.852 ± 0.022	0.835LysLys: 0.835 ± 0.027	1.86LysLeu: 1.86 ± 0.03	0.375LysMet: 0.375 ± 0.012	0.556LysAsn: 0.556 ± 0.017	1.381LysPro: 1.381 ± 0.034	0.665LysGln: 0.665 ± 0.017	1.403LysArg: 1.403 ± 0.025	1.087LysSer: 1.087 ± 0.025	1.354LysThr: 1.354 ± 0.029	1.747LysVal: 1.747 ± 0.035	0.24LysTrp: 0.24 ± 0.01	0.39LysTyr: 0.39 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	15.551LeuAla: 15.551 ± 0.105	0.785LeuCys: 0.785 ± 0.017	6.765LeuAsp: 6.765 ± 0.061	4.323LeuGlu: 4.323 ± 0.049	2.578LeuPhe: 2.578 ± 0.038	9.309LeuGly: 9.309 ± 0.074	2.149LeuHis: 2.149 ± 0.029	3.038LeuIle: 3.038 ± 0.04	1.964LeuLys: 1.964 ± 0.036	11.218LeuLeu: 11.218 ± 0.102	1.599LeuMet: 1.599 ± 0.025	1.533LeuAsn: 1.533 ± 0.024	6.64LeuPro: 6.64 ± 0.061	1.983LeuGln: 1.983 ± 0.034	8.71LeuArg: 8.71 ± 0.077	5.038LeuSer: 5.038 ± 0.056	6.957LeuThr: 6.957 ± 0.066	8.8LeuVal: 8.8 ± 0.073	1.289LeuTrp: 1.289 ± 0.027	1.838LeuTyr: 1.838 ± 0.03	0.0LeuXaa: 0.0 ± 0.0
Met	2.22MetAla: 2.22 ± 0.034	0.134MetCys: 0.134 ± 0.008	0.9MetAsp: 0.9 ± 0.021	0.745MetGlu: 0.745 ± 0.019	0.416MetPhe: 0.416 ± 0.014	1.271MetGly: 1.271 ± 0.021	0.321MetHis: 0.321 ± 0.012	0.671MetIle: 0.671 ± 0.019	0.442MetLys: 0.442 ± 0.015	1.655MetLeu: 1.655 ± 0.03	0.272MetMet: 0.272 ± 0.012	0.446MetAsn: 0.446 ± 0.016	1.157MetPro: 1.157 ± 0.024	0.397MetGln: 0.397 ± 0.015	1.425MetArg: 1.425 ± 0.025	1.277MetSer: 1.277 ± 0.027	1.576MetThr: 1.576 ± 0.026	1.241MetVal: 1.241 ± 0.024	0.212MetTrp: 0.212 ± 0.01	0.327MetTyr: 0.327 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.118AsnAla: 2.118 ± 0.039	0.149AsnCys: 0.149 ± 0.008	0.882AsnAsp: 0.882 ± 0.02	0.768AsnGlu: 0.768 ± 0.021	0.434AsnPhe: 0.434 ± 0.014	1.843AsnGly: 1.843 ± 0.036	0.385AsnHis: 0.385 ± 0.015	0.574AsnIle: 0.574 ± 0.018	0.392AsnLys: 0.392 ± 0.015	1.537AsnLeu: 1.537 ± 0.027	0.285AsnMet: 0.285 ± 0.01	0.401AsnAsn: 0.401 ± 0.017	1.315AsnPro: 1.315 ± 0.023	0.471AsnGln: 0.471 ± 0.015	1.2AsnArg: 1.2 ± 0.027	0.833AsnSer: 0.833 ± 0.021	1.058AsnThr: 1.058 ± 0.028	1.252AsnVal: 1.252 ± 0.029	0.258AsnTrp: 0.258 ± 0.012	0.351AsnTyr: 0.351 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	10.264ProAla: 10.264 ± 0.1	0.387ProCys: 0.387 ± 0.014	4.692ProAsp: 4.692 ± 0.055	4.582ProGlu: 4.582 ± 0.051	1.544ProPhe: 1.544 ± 0.024	7.383ProGly: 7.383 ± 0.085	1.384ProHis: 1.384 ± 0.024	1.183ProIle: 1.183 ± 0.021	1.225ProLys: 1.225 ± 0.026	5.599ProLeu: 5.599 ± 0.053	1.013ProMet: 1.013 ± 0.022	0.851ProAsn: 0.851 ± 0.02	3.922ProPro: 3.922 ± 0.073	1.606ProGln: 1.606 ± 0.035	4.502ProArg: 4.502 ± 0.051	3.249ProSer: 3.249 ± 0.045	3.261ProThr: 3.261 ± 0.046	5.823ProVal: 5.823 ± 0.065	0.891ProTrp: 0.891 ± 0.02	1.445ProTyr: 1.445 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	3.705GlnAla: 3.705 ± 0.052	0.162GlnCys: 0.162 ± 0.008	1.349GlnAsp: 1.349 ± 0.026	1.357GlnGlu: 1.357 ± 0.025	0.584GlnPhe: 0.584 ± 0.014	2.283GlnGly: 2.283 ± 0.038	0.568GlnHis: 0.568 ± 0.016	0.964GlnIle: 0.964 ± 0.021	0.585GlnLys: 0.585 ± 0.019	2.601GlnLeu: 2.601 ± 0.036	0.434GlnMet: 0.434 ± 0.013	0.459GlnAsn: 0.459 ± 0.015	1.604GlnPro: 1.604 ± 0.031	1.085GlnGln: 1.085 ± 0.033	2.219GlnArg: 2.219 ± 0.029	1.172GlnSer: 1.172 ± 0.028	1.237GlnThr: 1.237 ± 0.026	2.13GlnVal: 2.13 ± 0.034	0.455GlnTrp: 0.455 ± 0.013	0.544GlnTyr: 0.544 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	10.602ArgAla: 10.602 ± 0.088	0.634ArgCys: 0.634 ± 0.018	4.477ArgAsp: 4.477 ± 0.053	4.708ArgGlu: 4.708 ± 0.052	2.323ArgPhe: 2.323 ± 0.037	6.151ArgGly: 6.151 ± 0.06	2.186ArgHis: 2.186 ± 0.035	3.261ArgIle: 3.261 ± 0.038	1.617ArgLys: 1.617 ± 0.028	9.045ArgLeu: 9.045 ± 0.072	1.817ArgMet: 1.817 ± 0.03	1.27ArgAsn: 1.27 ± 0.025	5.6ArgPro: 5.6 ± 0.055	2.184ArgGln: 2.184 ± 0.03	8.05ArgArg: 8.05 ± 0.082	3.868ArgSer: 3.868 ± 0.042	5.724ArgThr: 5.724 ± 0.053	6.12ArgVal: 6.12 ± 0.056	1.366ArgTrp: 1.366 ± 0.025	1.757ArgTyr: 1.757 ± 0.026	0.0ArgXaa: 0.0 ± 0.0
Ser	6.618SerAla: 6.618 ± 0.063	0.422SerCys: 0.422 ± 0.012	2.493SerAsp: 2.493 ± 0.034	2.208SerGlu: 2.208 ± 0.037	1.501SerPhe: 1.501 ± 0.03	5.903SerGly: 5.903 ± 0.05	0.923SerHis: 0.923 ± 0.02	1.281SerIle: 1.281 ± 0.025	0.95SerLys: 0.95 ± 0.024	4.633SerLeu: 4.633 ± 0.039	1.064SerMet: 1.064 ± 0.02	0.79SerAsn: 0.79 ± 0.021	3.291SerPro: 3.291 ± 0.042	1.079SerGln: 1.079 ± 0.026	3.635SerArg: 3.635 ± 0.041	2.728SerSer: 2.728 ± 0.042	2.977SerThr: 2.977 ± 0.046	4.052SerVal: 4.052 ± 0.042	0.86SerTrp: 0.86 ± 0.019	1.128SerTyr: 1.128 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	9.403ThrAla: 9.403 ± 0.074	0.443ThrCys: 0.443 ± 0.014	3.659ThrAsp: 3.659 ± 0.04	3.347ThrGlu: 3.347 ± 0.041	1.525ThrPhe: 1.525 ± 0.026	7.176ThrGly: 7.176 ± 0.067	1.243ThrHis: 1.243 ± 0.025	1.682ThrIle: 1.682 ± 0.028	1.14ThrLys: 1.14 ± 0.024	5.855ThrLeu: 5.855 ± 0.052	0.911ThrMet: 0.911 ± 0.022	0.943ThrAsn: 0.943 ± 0.03	4.602ThrPro: 4.602 ± 0.052	1.201ThrGln: 1.201 ± 0.025	4.284ThrArg: 4.284 ± 0.049	3.129ThrSer: 3.129 ± 0.045	4.158ThrThr: 4.158 ± 0.075	6.095ThrVal: 6.095 ± 0.059	0.912ThrTrp: 0.912 ± 0.022	1.353ThrTyr: 1.353 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	10.645ValAla: 10.645 ± 0.068	0.738ValCys: 0.738 ± 0.02	4.854ValAsp: 4.854 ± 0.049	4.498ValGlu: 4.498 ± 0.049	2.337ValPhe: 2.337 ± 0.036	6.252ValGly: 6.252 ± 0.057	1.92ValHis: 1.92 ± 0.031	2.55ValIle: 2.55 ± 0.038	1.702ValLys: 1.702 ± 0.032	9.298ValLeu: 9.298 ± 0.081	1.404ValMet: 1.404 ± 0.025	1.574ValAsn: 1.574 ± 0.028	5.587ValPro: 5.587 ± 0.058	1.931ValGln: 1.931 ± 0.027	7.307ValArg: 7.307 ± 0.061	4.179ValSer: 4.179 ± 0.042	5.821ValThr: 5.821 ± 0.067	7.781ValVal: 7.781 ± 0.074	1.144ValTrp: 1.144 ± 0.023	1.588ValTyr: 1.588 ± 0.027	0.0ValXaa: 0.0 ± 0.0
Trp	1.668TrpAla: 1.668 ± 0.029	0.162TrpCys: 0.162 ± 0.01	0.816TrpAsp: 0.816 ± 0.019	0.723TrpGlu: 0.723 ± 0.018	0.499TrpPhe: 0.499 ± 0.017	1.054TrpGly: 1.054 ± 0.023	0.352TrpHis: 0.352 ± 0.012	0.557TrpIle: 0.557 ± 0.015	0.351TrpLys: 0.351 ± 0.013	1.682TrpLeu: 1.682 ± 0.029	0.291TrpMet: 0.291 ± 0.011	0.406TrpAsn: 0.406 ± 0.015	0.822TrpPro: 0.822 ± 0.019	0.566TrpGln: 0.566 ± 0.017	1.358TrpArg: 1.358 ± 0.027	0.935TrpSer: 0.935 ± 0.023	1.072TrpThr: 1.072 ± 0.025	0.956TrpVal: 0.956 ± 0.019	0.337TrpTrp: 0.337 ± 0.014	0.325TrpTyr: 0.325 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.828TyrAla: 2.828 ± 0.032	0.166TyrCys: 0.166 ± 0.008	1.464TyrAsp: 1.464 ± 0.037	1.286TyrGlu: 1.286 ± 0.023	0.611TyrPhe: 0.611 ± 0.017	2.316TyrGly: 2.316 ± 0.036	0.362TyrHis: 0.362 ± 0.013	0.442TyrIle: 0.442 ± 0.016	0.392TyrLys: 0.392 ± 0.013	1.988TyrLeu: 1.988 ± 0.027	0.255TyrMet: 0.255 ± 0.011	0.361TyrAsn: 0.361 ± 0.015	1.057TyrPro: 1.057 ± 0.022	0.584TyrGln: 0.584 ± 0.016	1.759TyrArg: 1.759 ± 0.03	0.867TyrSer: 0.867 ± 0.021	1.222TyrThr: 1.222 ± 0.024	1.651TyrVal: 1.651 ± 0.029	0.337TyrTrp: 0.337 ± 0.013	0.391TyrTyr: 0.391 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7440 proteins (2333776 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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