Streptococcus satellite phage Javan541
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZUY0|A0A4D5ZUY0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan541 OX=2558735 GN=JavanS541_0011 PE=4 SV=1
MM1 pKa = 6.87 ITYY4 pKa = 10.54 DD5 pKa = 3.65 EE6 pKa = 4.97 FKK8 pKa = 10.84 EE9 pKa = 4.47 AMDD12 pKa = 3.61 KK13 pKa = 11.49 GFIKK17 pKa = 10.63 GDD19 pKa = 3.66 TIQIVRR25 pKa = 11.84 KK26 pKa = 9.1 NGKK29 pKa = 8.07 IHH31 pKa = 7.71 DD32 pKa = 4.19 YY33 pKa = 10.87 VLDD36 pKa = 3.85 GEE38 pKa = 4.38 RR39 pKa = 11.84 VEE41 pKa = 4.18 PHH43 pKa = 5.66 EE44 pKa = 4.23 TLSLEE49 pKa = 4.17 KK50 pKa = 10.83 VSDD53 pKa = 3.91 IIKK56 pKa = 10.47 EE57 pKa = 4.0 LGGDD61 pKa = 3.47 NN62 pKa = 4.05
Molecular weight: 7.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.879
IPC2_protein 4.876
IPC_protein 4.711
Toseland 4.584
ProMoST 4.838
Dawson 4.685
Bjellqvist 4.825
Wikipedia 4.558
Rodwell 4.571
Grimsley 4.507
Solomon 4.673
Lehninger 4.635
Nozaki 4.8
DTASelect 4.952
Thurlkill 4.596
EMBOSS 4.584
Sillero 4.851
Patrickios 4.253
IPC_peptide 4.685
IPC2_peptide 4.838
IPC2.peptide.svr19 4.827
Protein with the highest isoelectric point:
>tr|A0A4D5ZSB1|A0A4D5ZSB1_9VIRU Helicase loader OS=Streptococcus satellite phage Javan541 OX=2558735 GN=JavanS541_0006 PE=4 SV=1
MM1 pKa = 7.78 KK2 pKa = 10.26 INEE5 pKa = 4.22 IKK7 pKa = 10.67 KK8 pKa = 9.76 KK9 pKa = 10.54 DD10 pKa = 3.3 GSTVYY15 pKa = 10.36 RR16 pKa = 11.84 ANVYY20 pKa = 10.55 LGVDD24 pKa = 3.12 AVTGKK29 pKa = 10.68 KK30 pKa = 7.89 VTTKK34 pKa = 9.54 VTGRR38 pKa = 11.84 TKK40 pKa = 10.85 KK41 pKa = 9.77 EE42 pKa = 4.15 LKK44 pKa = 9.69 TKK46 pKa = 10.1 AQQAQFDD53 pKa = 4.54 FKK55 pKa = 11.59 ANGSTRR61 pKa = 11.84 YY62 pKa = 9.61 KK63 pKa = 9.67 EE64 pKa = 4.05 AAIEE68 pKa = 4.2 TYY70 pKa = 9.95 EE71 pKa = 4.36 ALAASWWDD79 pKa = 3.44 SYY81 pKa = 11.82 KK82 pKa = 10.19 NTVKK86 pKa = 10.96 ANTRR90 pKa = 11.84 KK91 pKa = 6.82 TQKK94 pKa = 10.49 VLLNNHH100 pKa = 5.76 VLPLFGGFKK109 pKa = 10.16 LDD111 pKa = 4.12 KK112 pKa = 9.92 LTTPLIQSIMNKK124 pKa = 9.72 LANNTNTGEE133 pKa = 4.06 VGAYY137 pKa = 8.95 LHH139 pKa = 6.53 YY140 pKa = 11.13 DD141 pKa = 3.96 KK142 pKa = 11.06 IHH144 pKa = 6.04 TLNKK148 pKa = 10.13 RR149 pKa = 11.84 ILQYY153 pKa = 11.17 GVILQVIPANPADD166 pKa = 3.9 NVVLPRR172 pKa = 11.84 NTQKK176 pKa = 11.1 DD177 pKa = 3.22 KK178 pKa = 10.56 KK179 pKa = 10.89 AKK181 pKa = 9.81 VKK183 pKa = 10.6 HH184 pKa = 5.76 FNNDD188 pKa = 2.8 EE189 pKa = 4.08 LKK191 pKa = 10.82 QFLTYY196 pKa = 10.54 LDD198 pKa = 4.21 SLDD201 pKa = 3.38 NTKK204 pKa = 10.86 YY205 pKa = 8.96 NTYY208 pKa = 10.95 YY209 pKa = 10.97 DD210 pKa = 3.1 ITLYY214 pKa = 10.96 KK215 pKa = 10.3 FLLATGCRR223 pKa = 11.84 INEE226 pKa = 4.0 ALALSWSDD234 pKa = 2.86 IDD236 pKa = 4.49 LDD238 pKa = 3.79 NSVVHH243 pKa = 5.55 ITKK246 pKa = 9.14 TLNCEE251 pKa = 4.0 MEE253 pKa = 4.45 LNSPKK258 pKa = 10.49 SKK260 pKa = 10.28 SGYY263 pKa = 9.46 RR264 pKa = 11.84 DD265 pKa = 2.85 IDD267 pKa = 3.16 IDD269 pKa = 3.82 QQTATMLKK277 pKa = 9.78 RR278 pKa = 11.84 YY279 pKa = 7.54 QRR281 pKa = 11.84 KK282 pKa = 6.4 QTQEE286 pKa = 3.04 AWKK289 pKa = 10.12 LGRR292 pKa = 11.84 TEE294 pKa = 4.07 TVVFSDD300 pKa = 5.04 FIKK303 pKa = 10.47 SYY305 pKa = 9.64 PSSKK309 pKa = 10.08 AASGRR314 pKa = 11.84 LRR316 pKa = 11.84 THH318 pKa = 6.57 FKK320 pKa = 10.46 KK321 pKa = 10.74 AGVNNIGFHH330 pKa = 5.79 GFRR333 pKa = 11.84 HH334 pKa = 4.87 THH336 pKa = 6.58 ASLLLNSGIPYY347 pKa = 10.0 KK348 pKa = 10.34 EE349 pKa = 3.8 LQHH352 pKa = 6.73 RR353 pKa = 11.84 LGHH356 pKa = 5.68 STLSMTMDD364 pKa = 4.17 TYY366 pKa = 11.8 SHH368 pKa = 7.15 LSKK371 pKa = 11.01 EE372 pKa = 4.3 NAKK375 pKa = 10.31 KK376 pKa = 9.35 ATSFYY381 pKa = 10.1 EE382 pKa = 3.9 QALKK386 pKa = 10.8 SII388 pKa = 4.54
Molecular weight: 44.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.012
IPC2_protein 9.18
IPC_protein 9.048
Toseland 9.882
ProMoST 9.531
Dawson 10.087
Bjellqvist 9.721
Wikipedia 10.233
Rodwell 10.628
Grimsley 10.145
Solomon 10.101
Lehninger 10.058
Nozaki 9.867
DTASelect 9.721
Thurlkill 9.94
EMBOSS 10.277
Sillero 9.999
Patrickios 10.043
IPC_peptide 10.101
IPC2_peptide 8.17
IPC2.peptide.svr19 8.122
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15
0
15
2350
50
388
156.7
17.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.106 ± 0.452
0.511 ± 0.133
5.234 ± 0.422
8.298 ± 0.755
3.106 ± 0.365
4.468 ± 0.445
1.702 ± 0.278
6.17 ± 0.444
9.745 ± 0.652
10.511 ± 0.791
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.043 ± 0.209
6.894 ± 0.197
2.638 ± 0.273
4.085 ± 0.348
4.468 ± 0.571
5.787 ± 0.299
7.149 ± 0.542
4.809 ± 0.483
0.596 ± 0.145
4.681 ± 0.273
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here