gamma proteobacterium HdN1
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3683 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E1VMK5|E1VMK5_9GAMM Uncharacterized protein OS=gamma proteobacterium HdN1 OX=83406 GN=HDN1F_24640 PE=4 SV=1
MM1 pKa = 7.82 ASLLTLRR8 pKa = 11.84 FMMFSTFMMEE18 pKa = 4.88 DD19 pKa = 3.1 SSLSLAYY26 pKa = 10.05 RR27 pKa = 11.84 PSFAGGNSGLSFGHH41 pKa = 5.95 FQHH44 pKa = 7.25 DD45 pKa = 3.61 VAANGDD51 pKa = 3.32 ARR53 pKa = 11.84 ATFEE57 pKa = 5.21 SILEE61 pKa = 4.0 QSGLFDD67 pKa = 4.01 DD68 pKa = 5.55 QEE70 pKa = 4.9 INDD73 pKa = 4.07 IVDD76 pKa = 4.0 LASTPGVRR84 pKa = 11.84 ASDD87 pKa = 3.74 FTSSQLSRR95 pKa = 11.84 ITQALSSNLGKK106 pKa = 8.63 QTVDD110 pKa = 4.0 QRR112 pKa = 11.84 DD113 pKa = 2.89 ADD115 pKa = 4.1 YY116 pKa = 11.21 FNDD119 pKa = 3.76 TLRR122 pKa = 11.84 PWIEE126 pKa = 4.01 SALNAANQNPNGPGVFSPVNADD148 pKa = 3.46 YY149 pKa = 9.19 LTALSMLAAWVNRR162 pKa = 11.84 TGAPNLLSQYY172 pKa = 10.88 LRR174 pKa = 11.84 GQSVTLGGVTLQLNGAPSLLGFQNYY199 pKa = 7.35 LTHH202 pKa = 6.21 TQQFRR207 pKa = 11.84 PEE209 pKa = 4.16 SQGGNGEE216 pKa = 4.27 NFGNWMDD223 pKa = 5.05 RR224 pKa = 11.84 IYY226 pKa = 11.28 DD227 pKa = 3.6 AVDD230 pKa = 3.05 YY231 pKa = 10.72 GIEE234 pKa = 3.96 KK235 pKa = 9.9 TGIDD239 pKa = 4.1 DD240 pKa = 4.96 SYY242 pKa = 11.74 DD243 pKa = 3.28 IDD245 pKa = 5.68 GDD247 pKa = 4.12 GLHH250 pKa = 6.84 NDD252 pKa = 3.62 IDD254 pKa = 4.91 DD255 pKa = 5.94 DD256 pKa = 4.42 IDD258 pKa = 5.65 GDD260 pKa = 4.42 GIANFLDD267 pKa = 3.95 STPEE271 pKa = 3.51 GDD273 pKa = 3.27 IFRR276 pKa = 11.84 DD277 pKa = 3.15 IDD279 pKa = 3.59 GDD281 pKa = 4.35 GIIDD285 pKa = 4.24 SEE287 pKa = 4.3 DD288 pKa = 2.92 WDD290 pKa = 3.89 MDD292 pKa = 3.4 GDD294 pKa = 4.82 GIPNVGDD301 pKa = 3.52 STPEE305 pKa = 4.0 GEE307 pKa = 4.96 VDD309 pKa = 3.91 PEE311 pKa = 4.55 LPPTDD316 pKa = 5.04 DD317 pKa = 4.6 PEE319 pKa = 5.04 LPPTDD324 pKa = 5.04 DD325 pKa = 4.6 PEE327 pKa = 5.04 LPPTDD332 pKa = 5.04 DD333 pKa = 4.6 PEE335 pKa = 5.04 LPPTDD340 pKa = 5.04 DD341 pKa = 4.6 PEE343 pKa = 5.04 LPPTDD348 pKa = 5.04 DD349 pKa = 4.6 PEE351 pKa = 5.04 LPPTDD356 pKa = 5.56 DD357 pKa = 4.66 PDD359 pKa = 3.83 NDD361 pKa = 3.69 SDD363 pKa = 4.4 YY364 pKa = 11.32 NPPPGDD370 pKa = 4.37 LDD372 pKa = 4.8 DD373 pKa = 6.64 DD374 pKa = 5.06 GDD376 pKa = 4.72 GDD378 pKa = 4.55 LTDD381 pKa = 4.44 IDD383 pKa = 4.17 IDD385 pKa = 4.81 GDD387 pKa = 4.03 DD388 pKa = 4.57 FPNNSDD394 pKa = 4.71 DD395 pKa = 6.57 DD396 pKa = 4.78 PYY398 pKa = 11.75 DD399 pKa = 3.38 WNNPLPQPDD408 pKa = 3.64 TDD410 pKa = 4.11 GDD412 pKa = 4.11 GFPDD416 pKa = 4.71 HH417 pKa = 7.21 VDD419 pKa = 3.74 DD420 pKa = 5.34 YY421 pKa = 11.29 PWGPDD426 pKa = 3.12 GPADD430 pKa = 3.83 MDD432 pKa = 4.5 HH433 pKa = 7.7 DD434 pKa = 4.58 GLPDD438 pKa = 4.75 DD439 pKa = 5.41 GSPIPLPPPPPPPPPGPTPSSPLVLDD465 pKa = 4.2 LDD467 pKa = 4.3 GDD469 pKa = 4.23 GVEE472 pKa = 4.6 TVGADD477 pKa = 2.9 SDD479 pKa = 3.98 IYY481 pKa = 10.85 FDD483 pKa = 3.78 HH484 pKa = 7.52 SGDD487 pKa = 3.75 GFSEE491 pKa = 4.12 LSGFVSPDD499 pKa = 3.48 DD500 pKa = 4.07 GLLALDD506 pKa = 4.09 RR507 pKa = 11.84 NGDD510 pKa = 3.6 GFINSGQEE518 pKa = 3.57 LFGNFTILQDD528 pKa = 3.46 GTRR531 pKa = 11.84 AANGFAALQEE541 pKa = 4.27 MDD543 pKa = 3.87 TNHH546 pKa = 7.06 DD547 pKa = 3.92 GVVDD551 pKa = 4.26 GQDD554 pKa = 3.24 GGFSALRR561 pKa = 11.84 VFRR564 pKa = 11.84 DD565 pKa = 3.42 SNQNGVTDD573 pKa = 4.45 DD574 pKa = 4.61 GEE576 pKa = 4.67 LLTLEE581 pKa = 4.24 QAGVTSLSLGYY592 pKa = 9.13 TSQSYY597 pKa = 10.92 VDD599 pKa = 3.89 AEE601 pKa = 4.59 GNEE604 pKa = 3.87 HH605 pKa = 6.1 RR606 pKa = 11.84 QVGSYY611 pKa = 6.99 TTTSGQSLALTDD623 pKa = 3.24 VWFDD627 pKa = 4.0 SNPTLSRR634 pKa = 11.84 EE635 pKa = 4.12 EE636 pKa = 4.38 EE637 pKa = 3.91 ISVSEE642 pKa = 5.48 DD643 pKa = 2.87 ISLLPNATGYY653 pKa = 8.06 GTAHH657 pKa = 7.06 SLHH660 pKa = 5.79 QAIARR665 pKa = 11.84 DD666 pKa = 3.73 EE667 pKa = 4.2 TGEE670 pKa = 4.15 LLSLVQQFVSSTSRR684 pKa = 11.84 TEE686 pKa = 3.63 RR687 pKa = 11.84 LALVEE692 pKa = 4.68 EE693 pKa = 4.85 ILYY696 pKa = 10.42 SWTGQEE702 pKa = 3.41 GDD704 pKa = 3.45 FRR706 pKa = 11.84 QFYY709 pKa = 10.12 QSPIDD714 pKa = 3.52 ARR716 pKa = 11.84 KK717 pKa = 9.8 IGALEE722 pKa = 3.73 AFYY725 pKa = 10.19 GYY727 pKa = 10.86 NVDD730 pKa = 3.7 RR731 pKa = 11.84 PRR733 pKa = 11.84 GSGQQYY739 pKa = 8.36 SRR741 pKa = 11.84 MYY743 pKa = 10.23 EE744 pKa = 4.18 GYY746 pKa = 10.37 FEE748 pKa = 4.84 NLVNGVFYY756 pKa = 10.48 EE757 pKa = 4.83 LSAQSYY763 pKa = 8.47 LKK765 pKa = 10.64 PFFAKK770 pKa = 10.63 VSWSYY775 pKa = 10.99 NAEE778 pKa = 3.83 SDD780 pKa = 3.39 VWEE783 pKa = 4.76 GNFSRR788 pKa = 11.84 VVKK791 pKa = 10.65 DD792 pKa = 4.17 LFDD795 pKa = 4.18 FAQANPAEE803 pKa = 4.44 AQSLLSDD810 pKa = 4.35 FAQSVLGVNVYY821 pKa = 10.51 NRR823 pKa = 11.84 TNIDD827 pKa = 3.55 SLLATIDD834 pKa = 4.36 LFVQSDD840 pKa = 4.89 LISAYY845 pKa = 10.55 SPEE848 pKa = 4.21 AVSLVLAATMEE859 pKa = 4.22 NVIEE863 pKa = 4.31 EE864 pKa = 4.2 NGVIYY869 pKa = 10.75 GDD871 pKa = 3.73 AGNNYY876 pKa = 9.98 LFGLDD881 pKa = 4.98 GNDD884 pKa = 3.37 QLSGNAGNDD893 pKa = 3.38 VLDD896 pKa = 4.72 GGTGDD901 pKa = 4.42 DD902 pKa = 3.91 VLIGGSGNDD911 pKa = 3.13 QFRR914 pKa = 11.84 FGVGYY919 pKa = 9.77 GHH921 pKa = 7.46 DD922 pKa = 3.5 RR923 pKa = 11.84 VRR925 pKa = 11.84 TQDD928 pKa = 3.46 TVTGRR933 pKa = 11.84 YY934 pKa = 9.22 DD935 pKa = 3.33 VISIVGGITAADD947 pKa = 3.52 VSITRR952 pKa = 11.84 QADD955 pKa = 3.54 DD956 pKa = 4.14 LVISILGTDD965 pKa = 3.26 DD966 pKa = 3.3 VLRR969 pKa = 11.84 VEE971 pKa = 4.63 GHH973 pKa = 6.39 FSQEE977 pKa = 4.37 GASHH981 pKa = 6.73 SYY983 pKa = 10.14 VDD985 pKa = 5.19 AIVFSDD991 pKa = 3.51 GTTINVGVSEE1001 pKa = 4.37 FNNINVSSQVISDD1014 pKa = 4.05 ADD1016 pKa = 3.79 NEE1018 pKa = 4.29 IHH1020 pKa = 6.12 GTSDD1024 pKa = 3.2 NDD1026 pKa = 4.09 VINALAGNDD1035 pKa = 3.41 QVFGKK1040 pKa = 8.97 TGDD1043 pKa = 3.74 DD1044 pKa = 4.19 EE1045 pKa = 4.67 ILGGAGNDD1053 pKa = 4.1 AISGDD1058 pKa = 3.63 GGADD1062 pKa = 3.59 RR1063 pKa = 11.84 IFGGTGNDD1071 pKa = 3.35 QLSGGTEE1078 pKa = 3.97 DD1079 pKa = 3.89 DD1080 pKa = 3.61 VLYY1083 pKa = 11.06 GEE1085 pKa = 5.16 SGHH1088 pKa = 6.67 DD1089 pKa = 3.69 TLSGDD1094 pKa = 3.56 AGNDD1098 pKa = 3.02 ILVGSTGDD1106 pKa = 3.61 DD1107 pKa = 3.75 LLIGGAGNDD1116 pKa = 2.54 IYY1118 pKa = 11.13 LFNVGDD1124 pKa = 4.06 GLDD1127 pKa = 3.74 VIDD1130 pKa = 5.56 NRR1132 pKa = 11.84 GSSNDD1137 pKa = 3.69 DD1138 pKa = 3.11 DD1139 pKa = 4.13 VLRR1142 pKa = 11.84 LGEE1145 pKa = 4.59 GIRR1148 pKa = 11.84 SQDD1151 pKa = 3.14 VKK1153 pKa = 10.82 LWRR1156 pKa = 11.84 SGNNLVIEE1164 pKa = 4.58 VNSGTDD1170 pKa = 3.43 EE1171 pKa = 4.31 ISVSNYY1177 pKa = 9.02 FSPNAPSIDD1186 pKa = 3.42 RR1187 pKa = 11.84 IIFQDD1192 pKa = 3.33 AGSEE1196 pKa = 4.08 LSEE1199 pKa = 3.89 ITSAQIEE1206 pKa = 4.85 VIVKK1210 pKa = 10.06 SATEE1214 pKa = 4.19 GSDD1217 pKa = 3.43 QLHH1220 pKa = 5.6 GTAGIDD1226 pKa = 4.08 VFDD1229 pKa = 4.83 GLNGDD1234 pKa = 3.77 DD1235 pKa = 4.41 TIYY1238 pKa = 11.05 GHH1240 pKa = 7.51 DD1241 pKa = 4.26 GNDD1244 pKa = 3.51 TLSGSGGNDD1253 pKa = 2.95 TLMGGYY1259 pKa = 10.67 ANDD1262 pKa = 3.46 VLRR1265 pKa = 11.84 GGEE1268 pKa = 4.49 GNDD1271 pKa = 3.65 SLNGEE1276 pKa = 4.48 NGNDD1280 pKa = 3.67 TLDD1283 pKa = 3.73 GGVGDD1288 pKa = 5.42 DD1289 pKa = 4.28 YY1290 pKa = 11.78 LSGGAGSDD1298 pKa = 3.45 TYY1300 pKa = 11.37 LIDD1303 pKa = 4.26 ANSGSDD1309 pKa = 3.71 VIQDD1313 pKa = 3.41 YY1314 pKa = 10.73 DD1315 pKa = 3.59 ATGNDD1320 pKa = 3.32 YY1321 pKa = 11.52 DD1322 pKa = 4.21 KK1323 pKa = 11.6 VSFGSGITAQNLTFSRR1339 pKa = 11.84 KK1340 pKa = 9.67 NNDD1343 pKa = 2.52 VCVTVNYY1350 pKa = 10.85 NGDD1353 pKa = 3.17 QSSFVIANGFANAASMVDD1371 pKa = 4.08 GFWFSNGWYY1380 pKa = 8.58 LTGTSALAHH1389 pKa = 6.81 AAANWNGLDD1398 pKa = 3.47 SAEE1401 pKa = 4.04 VMNGYY1406 pKa = 9.69 SGNDD1410 pKa = 3.44 TIYY1413 pKa = 11.3 GNGGDD1418 pKa = 4.45 DD1419 pKa = 4.78 NINGGDD1425 pKa = 3.72 GSDD1428 pKa = 3.58 VLVGGHH1434 pKa = 6.27 GNDD1437 pKa = 3.86 YY1438 pKa = 9.68 LTGGAGRR1445 pKa = 11.84 DD1446 pKa = 3.45 TYY1448 pKa = 11.23 RR1449 pKa = 11.84 FDD1451 pKa = 4.02 IGDD1454 pKa = 3.61 GHH1456 pKa = 6.78 DD1457 pKa = 4.13 VISGSTNLNEE1467 pKa = 4.84 DD1468 pKa = 3.62 RR1469 pKa = 11.84 LEE1471 pKa = 4.49 LGSTTSLGNVGFRR1484 pKa = 11.84 RR1485 pKa = 11.84 VGDD1488 pKa = 3.69 NLVVYY1493 pKa = 10.21 LDD1495 pKa = 3.97 SNSTITVQSYY1505 pKa = 8.89 FSANDD1510 pKa = 3.42 PARR1513 pKa = 11.84 ALSSIRR1519 pKa = 11.84 LGDD1522 pKa = 3.47 GTVLSRR1528 pKa = 11.84 SDD1530 pKa = 3.68 VIANLPRR1537 pKa = 11.84 TGTSQADD1544 pKa = 4.02 AITLIGEE1551 pKa = 4.18 VDD1553 pKa = 3.91 DD1554 pKa = 4.87 SVSGLDD1560 pKa = 3.78 GDD1562 pKa = 5.08 DD1563 pKa = 4.7 SIAAMGGNDD1572 pKa = 3.78 TVNGNAGNDD1581 pKa = 3.48 RR1582 pKa = 11.84 LDD1584 pKa = 3.56 GGFGNDD1590 pKa = 2.87 IVVGGDD1596 pKa = 3.53 GQDD1599 pKa = 3.32 TLIGGTGSDD1608 pKa = 3.93 TLQGGYY1614 pKa = 10.85 GSDD1617 pKa = 3.19 TYY1619 pKa = 11.22 RR1620 pKa = 11.84 FNLGDD1625 pKa = 3.6 GQDD1628 pKa = 3.6 RR1629 pKa = 11.84 IEE1631 pKa = 4.33 EE1632 pKa = 4.13 NMASGAGTDD1641 pKa = 3.11 RR1642 pKa = 11.84 DD1643 pKa = 3.56 RR1644 pKa = 11.84 IVFGTGISEE1653 pKa = 4.07 NDD1655 pKa = 4.43 LIFSLAGRR1663 pKa = 11.84 DD1664 pKa = 3.55 LVITIGSGGDD1674 pKa = 3.15 QIVISNSYY1682 pKa = 10.77 ASDD1685 pKa = 3.16 NYY1687 pKa = 10.32 RR1688 pKa = 11.84 YY1689 pKa = 10.02 RR1690 pKa = 11.84 IEE1692 pKa = 4.08 EE1693 pKa = 4.28 LEE1695 pKa = 4.16 FADD1698 pKa = 4.02 GAVVNVATLLEE1709 pKa = 4.4 NNLVVGSYY1717 pKa = 10.42 VADD1720 pKa = 3.5 QLYY1723 pKa = 10.3 GADD1726 pKa = 3.82 GTNDD1730 pKa = 3.77 YY1731 pKa = 11.02 IGGADD1736 pKa = 5.26 GDD1738 pKa = 4.12 DD1739 pKa = 3.83 TLNGLTGNDD1748 pKa = 3.36 RR1749 pKa = 11.84 LMGGAGNDD1757 pKa = 3.43 TLMGGDD1763 pKa = 4.49 GNDD1766 pKa = 3.37 EE1767 pKa = 4.06 LAGGVGNDD1775 pKa = 3.98 SLQGSYY1781 pKa = 11.38 GSDD1784 pKa = 2.95 TYY1786 pKa = 11.31 RR1787 pKa = 11.84 FNQGDD1792 pKa = 3.78 GQDD1795 pKa = 3.51 WIEE1798 pKa = 4.85 EE1799 pKa = 4.15 YY1800 pKa = 9.91 MASNGTDD1807 pKa = 2.68 IDD1809 pKa = 4.32 RR1810 pKa = 11.84 IVFGAGIAVNDD1821 pKa = 4.06 LTLSTSGNHH1830 pKa = 6.04 LVITVGTNGDD1840 pKa = 3.8 SLTINNWYY1848 pKa = 8.81 TSSTYY1853 pKa = 10.11 RR1854 pKa = 11.84 VEE1856 pKa = 4.31 TFEE1859 pKa = 4.99 LADD1862 pKa = 3.82 GTVLTAQSLLEE1873 pKa = 3.78 GRR1875 pKa = 11.84 MVSGTEE1881 pKa = 3.45 TDD1883 pKa = 3.28 DD1884 pKa = 3.57 TVRR1887 pKa = 11.84 GNEE1890 pKa = 4.12 TYY1892 pKa = 11.06 NDD1894 pKa = 3.51 QLAGGGGNDD1903 pKa = 3.14 NLYY1906 pKa = 10.84 GYY1908 pKa = 10.02 GGNDD1912 pKa = 3.45 SLDD1915 pKa = 3.59 GGAGNDD1921 pKa = 3.67 TLSGEE1926 pKa = 4.4 EE1927 pKa = 4.74 GNDD1930 pKa = 3.47 TLIGGTGNDD1939 pKa = 4.04 TLQGSYY1945 pKa = 11.31 GSDD1948 pKa = 3.03 TYY1950 pKa = 11.26 RR1951 pKa = 11.84 FNLGYY1956 pKa = 10.0 GQDD1959 pKa = 3.64 RR1960 pKa = 11.84 IEE1962 pKa = 5.18 EE1963 pKa = 4.22 YY1964 pKa = 9.82 MASNGTDD1971 pKa = 2.89 VDD1973 pKa = 4.89 RR1974 pKa = 11.84 IAFGAGITANDD1985 pKa = 3.78 LALSVSGNNLVITVGTNGDD2004 pKa = 3.8 SLTINNWYY2012 pKa = 10.32 NSASYY2017 pKa = 10.03 RR2018 pKa = 11.84 VEE2020 pKa = 3.99 EE2021 pKa = 4.61 FEE2023 pKa = 5.37 LADD2026 pKa = 3.72 GTLLNAQSLLEE2037 pKa = 3.9 GRR2039 pKa = 11.84 GVSGSEE2045 pKa = 3.88 VAEE2048 pKa = 4.42 TIHH2051 pKa = 6.96 GNNSYY2056 pKa = 11.12 NDD2058 pKa = 3.47 QLAGRR2063 pKa = 11.84 GGNDD2067 pKa = 2.97 TLYY2070 pKa = 10.98 GYY2072 pKa = 11.05 AGDD2075 pKa = 4.88 DD2076 pKa = 3.96 LLDD2079 pKa = 4.44 GGTGDD2084 pKa = 4.24 DD2085 pKa = 3.97 TLSGAEE2091 pKa = 4.53 GNDD2094 pKa = 3.5 TLIGGVGNDD2103 pKa = 4.02 TLQGSYY2109 pKa = 11.31 GSDD2112 pKa = 3.03 TYY2114 pKa = 11.29 RR2115 pKa = 11.84 FNLGDD2120 pKa = 3.6 GQDD2123 pKa = 3.64 RR2124 pKa = 11.84 IEE2126 pKa = 5.24 EE2127 pKa = 4.18 YY2128 pKa = 9.82 MASNGTDD2135 pKa = 2.68 IDD2137 pKa = 4.32 RR2138 pKa = 11.84 IVFGAGITANDD2149 pKa = 3.78 LALSVSGNHH2158 pKa = 6.02 LVITIGNTGDD2168 pKa = 3.71 SLTINNWYY2176 pKa = 10.22 SSSTYY2181 pKa = 9.64 RR2182 pKa = 11.84 VEE2184 pKa = 4.22 EE2185 pKa = 4.14 FEE2187 pKa = 4.84 LADD2190 pKa = 3.69 GTVLNAQSLLEE2201 pKa = 3.87 GRR2203 pKa = 11.84 VVSGSEE2209 pKa = 3.87 SAEE2212 pKa = 3.99 TIHH2215 pKa = 7.16 GNNSYY2220 pKa = 11.12 NDD2222 pKa = 3.47 QLAGRR2227 pKa = 11.84 GGNDD2231 pKa = 2.97 TLYY2234 pKa = 10.98 GYY2236 pKa = 11.05 AGDD2239 pKa = 5.1 DD2240 pKa = 3.99 LLDD2243 pKa = 4.2 SGTGDD2248 pKa = 3.57 DD2249 pKa = 4.21 TLSGAEE2255 pKa = 4.66 GNDD2258 pKa = 3.51 SLIGGAGNDD2267 pKa = 3.67 ILQGSYY2273 pKa = 11.35 GSDD2276 pKa = 3.03 TYY2278 pKa = 11.29 RR2279 pKa = 11.84 FNLGDD2284 pKa = 3.6 GQDD2287 pKa = 3.64 RR2288 pKa = 11.84 IEE2290 pKa = 5.24 EE2291 pKa = 4.18 YY2292 pKa = 9.82 MASNGTDD2299 pKa = 2.68 IDD2301 pKa = 4.32 RR2302 pKa = 11.84 IVFGAGIFANDD2313 pKa = 3.57 LALSVSGNNLVITVGNSGDD2332 pKa = 3.74 SLTINNWYY2340 pKa = 10.11 SSSNYY2345 pKa = 9.01 RR2346 pKa = 11.84 VEE2348 pKa = 4.25 EE2349 pKa = 4.09 FEE2351 pKa = 4.83 LADD2354 pKa = 3.56 GTVLNTQSLLEE2365 pKa = 4.15 GFVVAGTDD2373 pKa = 3.8 AADD2376 pKa = 3.3 TVYY2379 pKa = 11.23 GNNAYY2384 pKa = 10.43 NDD2386 pKa = 3.61 QLAGGGGNDD2395 pKa = 3.14 NLYY2398 pKa = 10.88 GYY2400 pKa = 10.84 GGDD2403 pKa = 3.9 DD2404 pKa = 3.87 RR2405 pKa = 11.84 LNGGADD2411 pKa = 3.49 NDD2413 pKa = 4.5 TLSGAEE2419 pKa = 4.86 GNDD2422 pKa = 3.53 TLNGGIGNDD2431 pKa = 3.87 TLQGSYY2437 pKa = 11.28 GSDD2440 pKa = 3.28 TYY2442 pKa = 11.36 CFNLGDD2448 pKa = 3.86 GQDD2451 pKa = 3.64 RR2452 pKa = 11.84 IEE2454 pKa = 5.24 EE2455 pKa = 4.18 YY2456 pKa = 9.82 MASNGTDD2463 pKa = 2.68 IDD2465 pKa = 4.32 RR2466 pKa = 11.84 IVFGAGIAANDD2477 pKa = 3.81 LALSVSGNNLVITVGSNGDD2496 pKa = 3.57 SLTISNWYY2504 pKa = 9.54 SSSNYY2509 pKa = 9.01 RR2510 pKa = 11.84 VEE2512 pKa = 4.68 EE2513 pKa = 4.09 FEE2515 pKa = 5.83 LDD2517 pKa = 3.83 DD2518 pKa = 4.13 GTVLNAQSLLEE2529 pKa = 3.89 GHH2531 pKa = 5.94 VVSGNDD2537 pKa = 4.35 LANTLYY2543 pKa = 11.22 GNDD2546 pKa = 4.12 VVADD2550 pKa = 3.75 WLGGGGGNDD2559 pKa = 3.21 TLYY2562 pKa = 10.79 GYY2564 pKa = 10.16 SGNDD2568 pKa = 3.26 MLDD2571 pKa = 3.36 GGVGNDD2577 pKa = 3.72 TLVGGIGNDD2586 pKa = 3.44 TYY2588 pKa = 11.74 VVDD2591 pKa = 4.01 SLSDD2595 pKa = 3.54 VVTEE2599 pKa = 4.49 SASQGIDD2606 pKa = 3.2 TVNSSITWVLGTNLEE2621 pKa = 4.0 NLTLFGNGAINGTGNTLNNIITGNDD2646 pKa = 3.22 ADD2648 pKa = 3.98 NVLTGGRR2655 pKa = 11.84 GSDD2658 pKa = 3.36 VLIGRR2663 pKa = 11.84 KK2664 pKa = 10.06 GNDD2667 pKa = 3.11 TYY2669 pKa = 11.78 VVDD2672 pKa = 5.38 DD2673 pKa = 3.79 INYY2676 pKa = 9.8 QIVEE2680 pKa = 4.25 AEE2682 pKa = 4.11 AEE2684 pKa = 4.35 GTDD2687 pKa = 3.87 VVQSSLTWTLAQNVEE2702 pKa = 4.03 NLTLTGTTAINGTGNQQANSLIGNSASNRR2731 pKa = 11.84 LEE2733 pKa = 3.87 GLEE2736 pKa = 4.34 GNDD2739 pKa = 3.9 VLDD2742 pKa = 4.61 GGAGADD2748 pKa = 3.54 AMVGGLGDD2756 pKa = 4.63 DD2757 pKa = 3.4 IYY2759 pKa = 11.5 YY2760 pKa = 10.78 LEE2762 pKa = 4.26 NTADD2766 pKa = 3.9 KK2767 pKa = 10.88 VIEE2770 pKa = 4.02 LAQQGMDD2777 pKa = 3.81 TIYY2780 pKa = 11.23 SKK2782 pKa = 10.93 FSYY2785 pKa = 9.96 TLVDD2789 pKa = 3.3 NAEE2792 pKa = 4.04 NLVLQSGLFAFNATGNALDD2811 pKa = 3.86 NTITGNDD2818 pKa = 3.66 LYY2820 pKa = 11.75 GNIIDD2825 pKa = 4.75 GKK2827 pKa = 10.36 AGADD2831 pKa = 3.13 TMMGLGGGDD2840 pKa = 3.41 TYY2842 pKa = 11.17 IVDD2845 pKa = 3.66 NANDD2849 pKa = 3.61 QVIEE2853 pKa = 4.11 NVNAGTDD2860 pKa = 3.55 LVRR2863 pKa = 11.84 ASVTFSLSDD2872 pKa = 3.4 NVEE2875 pKa = 4.02 NLVLTGASAISGNGNQLNNQITGNSAANTLDD2906 pKa = 3.46 GKK2908 pKa = 10.7 EE2909 pKa = 4.22 GTDD2912 pKa = 3.27 ILTGGAGNDD2921 pKa = 3.44 AYY2923 pKa = 11.61 LMGRR2927 pKa = 11.84 GYY2929 pKa = 11.21 GSDD2932 pKa = 3.34 TIVEE2936 pKa = 4.02 NDD2938 pKa = 3.22 ALANTDD2944 pKa = 3.54 VLQFGQDD2951 pKa = 2.88 IAADD2955 pKa = 3.63 QLWFQRR2961 pKa = 11.84 VNADD2965 pKa = 3.91 LEE2967 pKa = 4.29 VSIIGTSDD2975 pKa = 2.89 KK2976 pKa = 10.43 VTISNWYY2983 pKa = 8.86 GGQANHH2989 pKa = 6.03 VEE2991 pKa = 4.23 QFTTAGGLSLSDD3003 pKa = 3.74 ASVDD3007 pKa = 3.54 ALVNAMAAFAPPAPGQVTLPQEE3029 pKa = 4.28 YY3030 pKa = 8.69 QQQLNGVIAANWRR3043 pKa = 3.6
Molecular weight: 319.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.49
IPC_protein 3.567
Toseland 3.312
ProMoST 3.745
Dawson 3.567
Bjellqvist 3.719
Wikipedia 3.528
Rodwell 3.376
Grimsley 3.223
Solomon 3.567
Lehninger 3.528
Nozaki 3.668
DTASelect 3.986
Thurlkill 3.376
EMBOSS 3.528
Sillero 3.694
Patrickios 1.38
IPC_peptide 3.567
IPC2_peptide 3.668
IPC2.peptide.svr19 3.662
Protein with the highest isoelectric point:
>tr|E1VP80|E1VP80_9GAMM Thiolase OS=gamma proteobacterium HdN1 OX=83406 GN=HDN1F_30390 PE=3 SV=1
MM1 pKa = 8.23 RR2 pKa = 11.84 DD3 pKa = 3.14 RR4 pKa = 11.84 LSWPKK9 pKa = 9.94 IYY11 pKa = 10.33 YY12 pKa = 9.64 VPSSGQVLKK21 pKa = 9.99 ATNIAPNKK29 pKa = 8.54 SQHH32 pKa = 5.11 LHH34 pKa = 5.42 ASVRR38 pKa = 11.84 GRR40 pKa = 11.84 RR41 pKa = 11.84 CAPPVCSALGQKK53 pKa = 7.39 WHH55 pKa = 7.26 RR56 pKa = 11.84 ATSGQGNEE64 pKa = 4.17 RR65 pKa = 11.84 FLSLGRR71 pKa = 11.84 FFQGFSVSLCCHH83 pKa = 6.74 CGASISRR90 pKa = 11.84 EE91 pKa = 3.7 VSAFSALRR99 pKa = 11.84 KK100 pKa = 9.68 NNHH103 pKa = 4.64 FQFWGGLYY111 pKa = 10.17 LQLVQWGAGVSS122 pKa = 3.51
Molecular weight: 13.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.706
IPC_protein 10.452
Toseland 10.599
ProMoST 10.292
Dawson 10.716
Bjellqvist 10.452
Wikipedia 10.921
Rodwell 10.965
Grimsley 10.774
Solomon 10.818
Lehninger 10.774
Nozaki 10.643
DTASelect 10.423
Thurlkill 10.613
EMBOSS 10.994
Sillero 10.657
Patrickios 10.716
IPC_peptide 10.818
IPC2_peptide 9.809
IPC2.peptide.svr19 8.41
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3683
0
3683
1343173
34
5494
364.7
40.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.328 ± 0.054
1.009 ± 0.015
5.215 ± 0.032
5.99 ± 0.039
3.869 ± 0.027
7.282 ± 0.044
2.346 ± 0.022
5.428 ± 0.03
4.117 ± 0.035
10.644 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.353 ± 0.019
3.513 ± 0.027
4.622 ± 0.029
4.491 ± 0.03
6.254 ± 0.038
6.262 ± 0.035
5.262 ± 0.038
6.908 ± 0.033
1.386 ± 0.018
2.72 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here