Amino acid dipepetide frequency for gamma proteobacterium HdN1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.193AlaAla: 11.193 ± 0.148
1.188AlaCys: 1.188 ± 0.034
4.935AlaAsp: 4.935 ± 0.059
6.241AlaGlu: 6.241 ± 0.073
3.691AlaPhe: 3.691 ± 0.057
7.595AlaGly: 7.595 ± 0.091
2.26AlaHis: 2.26 ± 0.045
5.757AlaIle: 5.757 ± 0.071
4.262AlaLys: 4.262 ± 0.068
12.177AlaLeu: 12.177 ± 0.14
2.706AlaMet: 2.706 ± 0.046
3.602AlaAsn: 3.602 ± 0.065
4.448AlaPro: 4.448 ± 0.071
4.578AlaGln: 4.578 ± 0.091
6.246AlaArg: 6.246 ± 0.085
6.73AlaSer: 6.73 ± 0.066
5.296AlaThr: 5.296 ± 0.068
6.474AlaVal: 6.474 ± 0.081
1.445AlaTrp: 1.445 ± 0.04
2.456AlaTyr: 2.456 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
1.002CysAla: 1.002 ± 0.029
0.131CysCys: 0.131 ± 0.01
0.533CysAsp: 0.533 ± 0.02
0.623CysGlu: 0.623 ± 0.022
0.433CysPhe: 0.433 ± 0.018
0.927CysGly: 0.927 ± 0.026
0.27CysHis: 0.27 ± 0.016
0.564CysIle: 0.564 ± 0.021
0.404CysLys: 0.404 ± 0.019
0.981CysLeu: 0.981 ± 0.031
0.217CysMet: 0.217 ± 0.014
0.337CysAsn: 0.337 ± 0.018
0.456CysPro: 0.456 ± 0.021
0.352CysGln: 0.352 ± 0.017
0.574CysArg: 0.574 ± 0.02
0.616CysSer: 0.616 ± 0.021
0.519CysThr: 0.519 ± 0.021
0.71CysVal: 0.71 ± 0.025
0.136CysTrp: 0.136 ± 0.011
0.305CysTyr: 0.305 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
5.809AspAla: 5.809 ± 0.068
0.508AspCys: 0.508 ± 0.021
2.579AspAsp: 2.579 ± 0.056
3.277AspGlu: 3.277 ± 0.066
2.225AspPhe: 2.225 ± 0.046
3.925AspGly: 3.925 ± 0.078
1.288AspHis: 1.288 ± 0.04
2.904AspIle: 2.904 ± 0.048
1.968AspLys: 1.968 ± 0.039
5.366AspLeu: 5.366 ± 0.074
1.103AspMet: 1.103 ± 0.032
1.57AspAsn: 1.57 ± 0.038
2.465AspPro: 2.465 ± 0.045
2.202AspGln: 2.202 ± 0.064
3.157AspArg: 3.157 ± 0.05
2.816AspSer: 2.816 ± 0.052
2.808AspThr: 2.808 ± 0.062
3.684AspVal: 3.684 ± 0.06
0.844AspTrp: 0.844 ± 0.031
1.653AspTyr: 1.653 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
5.911GluAla: 5.911 ± 0.076
0.491GluCys: 0.491 ± 0.018
2.749GluAsp: 2.749 ± 0.048
3.224GluGlu: 3.224 ± 0.063
2.161GluPhe: 2.161 ± 0.039
4.014GluGly: 4.014 ± 0.059
1.48GluHis: 1.48 ± 0.033
3.86GluIle: 3.86 ± 0.06
3.077GluLys: 3.077 ± 0.061
6.197GluLeu: 6.197 ± 0.088
1.5GluMet: 1.5 ± 0.034
2.286GluAsn: 2.286 ± 0.046
2.304GluPro: 2.304 ± 0.048
3.259GluGln: 3.259 ± 0.052
4.417GluArg: 4.417 ± 0.072
3.452GluSer: 3.452 ± 0.053
3.131GluThr: 3.131 ± 0.049
3.887GluVal: 3.887 ± 0.064
0.854GluTrp: 0.854 ± 0.029
1.642GluTyr: 1.642 ± 0.041
0.0GluXaa: 0.0 ± 0.0
Phe
3.784PheAla: 3.784 ± 0.053
0.521PheCys: 0.521 ± 0.019
2.429PheAsp: 2.429 ± 0.04
2.255PheGlu: 2.255 ± 0.053
1.698PhePhe: 1.698 ± 0.043
3.233PheGly: 3.233 ± 0.054
0.901PheHis: 0.901 ± 0.027
1.882PheIle: 1.882 ± 0.037
1.33PheLys: 1.33 ± 0.033
3.661PheLeu: 3.661 ± 0.064
0.88PheMet: 0.88 ± 0.029
1.46PheAsn: 1.46 ± 0.034
1.572PhePro: 1.572 ± 0.037
1.46PheGln: 1.46 ± 0.034
2.164PheArg: 2.164 ± 0.048
3.009PheSer: 3.009 ± 0.051
2.065PheThr: 2.065 ± 0.04
2.657PheVal: 2.657 ± 0.051
0.553PheTrp: 0.553 ± 0.022
1.171PheTyr: 1.171 ± 0.03
0.0PheXaa: 0.0 ± 0.0
Gly
6.817GlyAla: 6.817 ± 0.09
0.841GlyCys: 0.841 ± 0.026
3.703GlyAsp: 3.703 ± 0.074
4.378GlyGlu: 4.378 ± 0.061
3.296GlyPhe: 3.296 ± 0.051
5.519GlyGly: 5.519 ± 0.088
1.633GlyHis: 1.633 ± 0.036
4.445GlyIle: 4.445 ± 0.061
3.735GlyLys: 3.735 ± 0.06
7.241GlyLeu: 7.241 ± 0.12
2.048GlyMet: 2.048 ± 0.048
2.676GlyAsn: 2.676 ± 0.087
2.098GlyPro: 2.098 ± 0.043
2.844GlyGln: 2.844 ± 0.055
4.314GlyArg: 4.314 ± 0.065
4.269GlySer: 4.269 ± 0.071
3.677GlyThr: 3.677 ± 0.067
5.735GlyVal: 5.735 ± 0.069
1.161GlyTrp: 1.161 ± 0.037
2.391GlyTyr: 2.391 ± 0.044
0.0GlyXaa: 0.0 ± 0.0
His
2.298HisAla: 2.298 ± 0.04
0.319HisCys: 0.319 ± 0.014
1.165HisAsp: 1.165 ± 0.041
1.28HisGlu: 1.28 ± 0.034
1.132HisPhe: 1.132 ± 0.032
1.738HisGly: 1.738 ± 0.039
0.707HisHis: 0.707 ± 0.022
1.156HisIle: 1.156 ± 0.033
0.81HisLys: 0.81 ± 0.031
2.513HisLeu: 2.513 ± 0.042
0.471HisMet: 0.471 ± 0.019
0.721HisAsn: 0.721 ± 0.022
1.366HisPro: 1.366 ± 0.033
1.095HisGln: 1.095 ± 0.024
1.45HisArg: 1.45 ± 0.034
1.389HisSer: 1.389 ± 0.03
1.176HisThr: 1.176 ± 0.03
1.35HisVal: 1.35 ± 0.032
0.45HisTrp: 0.45 ± 0.019
0.876HisTyr: 0.876 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
6.054IleAla: 6.054 ± 0.069
0.599IleCys: 0.599 ± 0.022
3.167IleAsp: 3.167 ± 0.057
3.634IleGlu: 3.634 ± 0.058
1.841IlePhe: 1.841 ± 0.044
4.197IleGly: 4.197 ± 0.062
1.266IleHis: 1.266 ± 0.031
2.487IleIle: 2.487 ± 0.053
2.215IleLys: 2.215 ± 0.061
4.643IleLeu: 4.643 ± 0.071
1.02IleMet: 1.02 ± 0.029
2.07IleAsn: 2.07 ± 0.046
2.718IlePro: 2.718 ± 0.048
2.192IleGln: 2.192 ± 0.043
3.487IleArg: 3.487 ± 0.058
3.783IleSer: 3.783 ± 0.069
3.304IleThr: 3.304 ± 0.053
3.748IleVal: 3.748 ± 0.066
0.525IleTrp: 0.525 ± 0.019
1.326IleTyr: 1.326 ± 0.034
0.0IleXaa: 0.0 ± 0.0
Lys
4.333LysAla: 4.333 ± 0.074
0.26LysCys: 0.26 ± 0.015
2.038LysAsp: 2.038 ± 0.046
2.371LysGlu: 2.371 ± 0.047
1.185LysPhe: 1.185 ± 0.029
2.977LysGly: 2.977 ± 0.069
0.931LysHis: 0.931 ± 0.031
2.357LysIle: 2.357 ± 0.045
2.064LysLys: 2.064 ± 0.051
4.189LysLeu: 4.189 ± 0.06
1.001LysMet: 1.001 ± 0.029
1.555LysAsn: 1.555 ± 0.039
2.26LysPro: 2.26 ± 0.053
1.913LysGln: 1.913 ± 0.041
2.697LysArg: 2.697 ± 0.06
2.478LysSer: 2.478 ± 0.049
2.42LysThr: 2.42 ± 0.047
2.806LysVal: 2.806 ± 0.051
0.409LysTrp: 0.409 ± 0.018
0.922LysTyr: 0.922 ± 0.028
0.0LysXaa: 0.0 ± 0.0
Leu
11.774LeuAla: 11.774 ± 0.103
1.101LeuCys: 1.101 ± 0.033
5.783LeuAsp: 5.783 ± 0.074
6.252LeuGlu: 6.252 ± 0.074
3.967LeuPhe: 3.967 ± 0.065
7.617LeuGly: 7.617 ± 0.091
2.32LeuHis: 2.32 ± 0.048
5.317LeuIle: 5.317 ± 0.075
4.393LeuLys: 4.393 ± 0.07
11.305LeuLeu: 11.305 ± 0.151
2.437LeuMet: 2.437 ± 0.046
3.635LeuAsn: 3.635 ± 0.059
5.553LeuPro: 5.553 ± 0.086
4.757LeuGln: 4.757 ± 0.07
6.836LeuArg: 6.836 ± 0.091
7.14LeuSer: 7.14 ± 0.108
5.458LeuThr: 5.458 ± 0.123
6.951LeuVal: 6.951 ± 0.095
1.372LeuTrp: 1.372 ± 0.039
2.475LeuTyr: 2.475 ± 0.04
0.0LeuXaa: 0.0 ± 0.0
Met
2.464MetAla: 2.464 ± 0.048
0.172MetCys: 0.172 ± 0.012
1.202MetAsp: 1.202 ± 0.036
1.251MetGlu: 1.251 ± 0.031
0.725MetPhe: 0.725 ± 0.021
1.68MetGly: 1.68 ± 0.041
0.491MetHis: 0.491 ± 0.02
1.215MetIle: 1.215 ± 0.031
1.021MetLys: 1.021 ± 0.03
2.614MetLeu: 2.614 ± 0.047
0.613MetMet: 0.613 ± 0.024
0.943MetAsn: 0.943 ± 0.025
1.323MetPro: 1.323 ± 0.035
1.148MetGln: 1.148 ± 0.033
1.553MetArg: 1.553 ± 0.035
1.528MetSer: 1.528 ± 0.035
1.292MetThr: 1.292 ± 0.032
1.674MetVal: 1.674 ± 0.043
0.183MetTrp: 0.183 ± 0.013
0.437MetTyr: 0.437 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
3.868AsnAla: 3.868 ± 0.064
0.347AsnCys: 0.347 ± 0.015
1.935AsnAsp: 1.935 ± 0.065
1.859AsnGlu: 1.859 ± 0.039
1.266AsnPhe: 1.266 ± 0.033
2.791AsnGly: 2.791 ± 0.071
0.875AsnHis: 0.875 ± 0.022
1.814AsnIle: 1.814 ± 0.058
1.228AsnLys: 1.228 ± 0.032
3.549AsnLeu: 3.549 ± 0.054
0.748AsnMet: 0.748 ± 0.025
1.261AsnAsn: 1.261 ± 0.039
2.137AsnPro: 2.137 ± 0.038
1.541AsnGln: 1.541 ± 0.035
2.142AsnArg: 2.142 ± 0.046
2.03AsnSer: 2.03 ± 0.04
2.069AsnThr: 2.069 ± 0.045
2.264AsnVal: 2.264 ± 0.044
0.449AsnTrp: 0.449 ± 0.016
0.958AsnTyr: 0.958 ± 0.032
0.0AsnXaa: 0.0 ± 0.0
Pro
4.863ProAla: 4.863 ± 0.075
0.364ProCys: 0.364 ± 0.016
2.679ProAsp: 2.679 ± 0.048
3.629ProGlu: 3.629 ± 0.065
1.827ProPhe: 1.827 ± 0.039
2.774ProGly: 2.774 ± 0.047
1.034ProHis: 1.034 ± 0.031
2.48ProIle: 2.48 ± 0.046
1.901ProLys: 1.901 ± 0.039
4.805ProLeu: 4.805 ± 0.069
1.141ProMet: 1.141 ± 0.031
1.693ProAsn: 1.693 ± 0.037
2.059ProPro: 2.059 ± 0.048
1.892ProGln: 1.892 ± 0.043
2.342ProArg: 2.342 ± 0.04
3.018ProSer: 3.018 ± 0.055
2.403ProThr: 2.403 ± 0.047
3.435ProVal: 3.435 ± 0.054
0.715ProTrp: 0.715 ± 0.024
1.17ProTyr: 1.17 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
4.906GlnAla: 4.906 ± 0.077
0.361GlnCys: 0.361 ± 0.016
1.834GlnAsp: 1.834 ± 0.04
2.511GlnGlu: 2.511 ± 0.045
1.581GlnPhe: 1.581 ± 0.034
2.925GlnGly: 2.925 ± 0.047
1.122GlnHis: 1.122 ± 0.029
2.528GlnIle: 2.528 ± 0.06
1.965GlnLys: 1.965 ± 0.044
4.842GlnLeu: 4.842 ± 0.077
1.024GlnMet: 1.024 ± 0.033
1.46GlnAsn: 1.46 ± 0.038
2.076GlnPro: 2.076 ± 0.038
2.745GlnGln: 2.745 ± 0.076
3.293GlnArg: 3.293 ± 0.062
2.455GlnSer: 2.455 ± 0.045
2.231GlnThr: 2.231 ± 0.042
3.114GlnVal: 3.114 ± 0.088
0.742GlnTrp: 0.742 ± 0.023
1.193GlnTyr: 1.193 ± 0.037
0.0GlnXaa: 0.0 ± 0.0
Arg
5.746ArgAla: 5.746 ± 0.081
0.605ArgCys: 0.605 ± 0.025
3.272ArgAsp: 3.272 ± 0.052
4.15ArgGlu: 4.15 ± 0.069
2.856ArgPhe: 2.856 ± 0.056
3.628ArgGly: 3.628 ± 0.052
1.601ArgHis: 1.601 ± 0.036
3.942ArgIle: 3.942 ± 0.06
2.805ArgLys: 2.805 ± 0.052
6.882ArgLeu: 6.882 ± 0.091
1.622ArgMet: 1.622 ± 0.03
2.35ArgAsn: 2.35 ± 0.046
2.408ArgPro: 2.408 ± 0.044
2.744ArgGln: 2.744 ± 0.05
3.812ArgArg: 3.812 ± 0.062
3.632ArgSer: 3.632 ± 0.048
2.988ArgThr: 2.988 ± 0.045
4.179ArgVal: 4.179 ± 0.06
1.056ArgTrp: 1.056 ± 0.037
2.265ArgTyr: 2.265 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
6.779SerAla: 6.779 ± 0.102
0.555SerCys: 0.555 ± 0.023
3.216SerAsp: 3.216 ± 0.065
3.648SerGlu: 3.648 ± 0.049
2.43SerPhe: 2.43 ± 0.041
5.223SerGly: 5.223 ± 0.075
1.406SerHis: 1.406 ± 0.033
3.226SerIle: 3.226 ± 0.052
2.364SerLys: 2.364 ± 0.05
6.724SerLeu: 6.724 ± 0.078
1.426SerMet: 1.426 ± 0.034
2.061SerAsn: 2.061 ± 0.039
2.726SerPro: 2.726 ± 0.05
2.656SerGln: 2.656 ± 0.048
3.646SerArg: 3.646 ± 0.048
4.066SerSer: 4.066 ± 0.071
3.475SerThr: 3.475 ± 0.077
4.535SerVal: 4.535 ± 0.063
0.783SerTrp: 0.783 ± 0.024
1.677SerTyr: 1.677 ± 0.047
0.0SerXaa: 0.0 ± 0.0
Thr
5.127ThrAla: 5.127 ± 0.097
0.465ThrCys: 0.465 ± 0.019
2.696ThrAsp: 2.696 ± 0.067
2.898ThrGlu: 2.898 ± 0.054
1.834ThrPhe: 1.834 ± 0.035
4.288ThrGly: 4.288 ± 0.069
1.348ThrHis: 1.348 ± 0.036
2.634ThrIle: 2.634 ± 0.048
1.567ThrLys: 1.567 ± 0.042
6.555ThrLeu: 6.555 ± 0.075
0.995ThrMet: 0.995 ± 0.028
1.581ThrAsn: 1.581 ± 0.041
3.276ThrPro: 3.276 ± 0.055
2.518ThrGln: 2.518 ± 0.053
3.157ThrArg: 3.157 ± 0.049
3.175ThrSer: 3.175 ± 0.067
2.882ThrThr: 2.882 ± 0.081
3.632ThrVal: 3.632 ± 0.059
0.652ThrTrp: 0.652 ± 0.022
1.345ThrTyr: 1.345 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
7.103ValAla: 7.103 ± 0.089
0.753ValCys: 0.753 ± 0.023
4.048ValAsp: 4.048 ± 0.063
4.314ValGlu: 4.314 ± 0.069
2.645ValPhe: 2.645 ± 0.043
4.878ValGly: 4.878 ± 0.067
1.47ValHis: 1.47 ± 0.033
3.794ValIle: 3.794 ± 0.062
2.642ValLys: 2.642 ± 0.05
7.307ValLeu: 7.307 ± 0.084
1.689ValMet: 1.689 ± 0.039
2.433ValAsn: 2.433 ± 0.042
3.075ValPro: 3.075 ± 0.058
2.662ValGln: 2.662 ± 0.049
4.23ValArg: 4.23 ± 0.061
4.515ValSer: 4.515 ± 0.062
3.534ValThr: 3.534 ± 0.056
5.465ValVal: 5.465 ± 0.073
0.831ValTrp: 0.831 ± 0.027
1.693ValTyr: 1.693 ± 0.038
0.0ValXaa: 0.0 ± 0.0
Trp
1.048TrpAla: 1.048 ± 0.028
0.17TrpCys: 0.17 ± 0.011
0.614TrpAsp: 0.614 ± 0.025
0.662TrpGlu: 0.662 ± 0.024
0.627TrpPhe: 0.627 ± 0.021
0.847TrpGly: 0.847 ± 0.028
0.355TrpHis: 0.355 ± 0.017
0.71TrpIle: 0.71 ± 0.026
0.56TrpLys: 0.56 ± 0.023
1.948TrpLeu: 1.948 ± 0.044
0.374TrpMet: 0.374 ± 0.018
0.473TrpAsn: 0.473 ± 0.021
0.573TrpPro: 0.573 ± 0.023
0.933TrpGln: 0.933 ± 0.031
0.986TrpArg: 0.986 ± 0.029
0.749TrpSer: 0.749 ± 0.028
0.548TrpThr: 0.548 ± 0.019
1.026TrpVal: 1.026 ± 0.028
0.254TrpTrp: 0.254 ± 0.012
0.411TrpTyr: 0.411 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.4TyrAla: 2.4 ± 0.043
0.339TyrCys: 0.339 ± 0.016
1.572TyrAsp: 1.572 ± 0.051
1.441TyrGlu: 1.441 ± 0.039
1.266TyrPhe: 1.266 ± 0.037
2.049TyrGly: 2.049 ± 0.041
0.701TyrHis: 0.701 ± 0.023
1.203TyrIle: 1.203 ± 0.03
0.854TyrLys: 0.854 ± 0.029
2.945TyrLeu: 2.945 ± 0.046
0.515TyrMet: 0.515 ± 0.022
0.965TyrAsn: 0.965 ± 0.033
1.33TyrPro: 1.33 ± 0.033
1.42TyrGln: 1.42 ± 0.039
2.04TyrArg: 2.04 ± 0.041
1.77TyrSer: 1.77 ± 0.044
1.347TyrThr: 1.347 ± 0.034
1.756TyrVal: 1.756 ± 0.041
0.451TyrTrp: 0.451 ± 0.02
0.838TyrTyr: 0.838 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3683 proteins (1343173 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski