Acetobacter aceti NBRC 14818

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Acetobacter; Acetobacter subgen. Acetobacter; Acetobacter aceti

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3146 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D6MUB1|A0A0D6MUB1_ACEAC Orotate phosphoribosyltransferase OS=Acetobacter aceti NBRC 14818 OX=887700 GN=pyrE PE=3 SV=1
MM1 pKa = 7.64SNLIADD7 pKa = 3.95IWNIGRR13 pKa = 11.84NIEE16 pKa = 3.97TSLIQSADD24 pKa = 3.47DD25 pKa = 3.73FLNDD29 pKa = 3.69TGINQIWSSLAALPSNILDD48 pKa = 3.33NTTPNNLLTFLNDD61 pKa = 3.21DD62 pKa = 3.67TAIPVPTHH70 pKa = 5.92QNLNQTPTVSQFLNAANTEE89 pKa = 4.16YY90 pKa = 11.42ALTGTPNGMSPFLVNGQQLAVIDD113 pKa = 4.35EE114 pKa = 4.44ASGMSAKK121 pKa = 10.15VWVTDD126 pKa = 3.32NDD128 pKa = 3.5QVIIAFQGTGGGFNAITNPAAVATGFLSDD157 pKa = 3.59VQIWNQSVSQAQKK170 pKa = 10.07DD171 pKa = 3.88ALSFTHH177 pKa = 5.96YY178 pKa = 10.49VVDD181 pKa = 3.93MAQKK185 pKa = 10.53QGIDD189 pKa = 3.36TNNIFVTGHH198 pKa = 5.54SLGGIEE204 pKa = 4.51ASYY207 pKa = 10.83VAQQTGLGGIAFEE220 pKa = 4.26PTGIPSSTTAKK231 pKa = 10.67GDD233 pKa = 3.42GSNFASVVTYY243 pKa = 10.23GDD245 pKa = 3.48PVGEE249 pKa = 4.29YY250 pKa = 10.97ANDD253 pKa = 3.72TMPGSSFVTSMPVGAAGDD271 pKa = 3.95FNHH274 pKa = 6.37YY275 pKa = 9.47GQVVMLGNPADD286 pKa = 4.1NAPLAAAVSNWNASSIGNVGEE307 pKa = 4.31LLSAASFAGYY317 pKa = 9.74HH318 pKa = 6.15LPGTQAADD326 pKa = 3.35MGITMTPYY334 pKa = 10.55VSLGDD339 pKa = 3.6TTHH342 pKa = 5.81YY343 pKa = 10.72QSGAVFNVGNDD354 pKa = 3.61SIAQLLTDD362 pKa = 3.62NADD365 pKa = 3.85RR366 pKa = 11.84TSSLASS372 pKa = 3.36

Molecular weight: 38.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D6MS39|A0A0D6MS39_ACEAC SnoaL-like domain-containing protein OS=Acetobacter aceti NBRC 14818 OX=887700 GN=Abac_003_080 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.54GFRR19 pKa = 11.84ARR21 pKa = 11.84TEE23 pKa = 4.29TVGGRR28 pKa = 11.84RR29 pKa = 11.84ILANRR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.5GRR39 pKa = 11.84KK40 pKa = 8.72RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3146

0

3146

1000029

40

3579

317.9

34.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.204 ± 0.056

1.023 ± 0.014

5.525 ± 0.033

5.509 ± 0.044

3.651 ± 0.03

8.306 ± 0.048

2.281 ± 0.02

5.191 ± 0.031

3.293 ± 0.036

10.268 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.551 ± 0.019

2.821 ± 0.033

5.352 ± 0.037

3.317 ± 0.025

6.776 ± 0.042

6.202 ± 0.04

5.808 ± 0.038

7.314 ± 0.035

1.39 ± 0.019

2.216 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski