Cucurbit yellow stunting disorder virus
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8JUU9|Q8JUU9_9CLOS 1b (Fragment) OS=Cucurbit yellow stunting disorder virus OX=51330 PE=4 SV=2
MM1 pKa = 7.34 LADD4 pKa = 3.91 EE5 pKa = 5.33 FGLAIIIALLIITLGVVCAYY25 pKa = 9.83 IGSCVYY31 pKa = 10.92 NHH33 pKa = 6.45 FFSDD37 pKa = 3.08 RR38 pKa = 11.84 DD39 pKa = 3.51 INIEE43 pKa = 3.9 EE44 pKa = 4.85 GEE46 pKa = 4.35 VSS48 pKa = 3.22
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.914
IPC2_protein 4.024
IPC_protein 3.783
Toseland 3.63
ProMoST 3.973
Dawson 3.77
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.643
Grimsley 3.567
Solomon 3.719
Lehninger 3.681
Nozaki 3.935
DTASelect 4.037
Thurlkill 3.719
EMBOSS 3.732
Sillero 3.91
Patrickios 0.172
IPC_peptide 3.719
IPC2_peptide 3.872
IPC2.peptide.svr19 3.822
Protein with the highest isoelectric point:
>tr|Q808I9|Q808I9_9CLOS p22 OS=Cucurbit yellow stunting disorder virus OX=51330 PE=4 SV=1
MM1 pKa = 7.33 YY2 pKa = 10.48 KK3 pKa = 10.25 NFSTIIFQFVCSRR16 pKa = 11.84 VKK18 pKa = 9.99 LWRR21 pKa = 11.84 RR22 pKa = 11.84 LVWILVLLSLLSAVMLMVLL41 pKa = 4.49
Molecular weight: 4.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.289
IPC2_protein 10.072
IPC_protein 11.052
Toseland 11.14
ProMoST 11.155
Dawson 11.199
Bjellqvist 11.008
Wikipedia 11.491
Rodwell 11.345
Grimsley 11.242
Solomon 11.462
Lehninger 11.403
Nozaki 11.14
DTASelect 11.008
Thurlkill 11.14
EMBOSS 11.579
Sillero 11.155
Patrickios 11.199
IPC_peptide 11.462
IPC2_peptide 10.335
IPC2.peptide.svr19 8.342
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13
0
13
5126
41
1977
394.3
45.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.824 ± 0.382
2.107 ± 0.248
6.535 ± 0.381
5.638 ± 0.333
5.638 ± 0.653
4.78 ± 0.396
1.99 ± 0.259
6.867 ± 0.317
8.369 ± 0.477
9.657 ± 0.638
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.653 ± 0.293
6.535 ± 0.446
3.102 ± 0.241
2.614 ± 0.256
4.76 ± 0.367
7.452 ± 0.393
5.17 ± 0.404
6.964 ± 0.314
0.702 ± 0.169
4.643 ± 0.43
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here