Flavobacterium kingsejongi
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3535 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S1LL23|A0A2S1LL23_9FLAO NAD(P)-dependent oxidoreductase OS=Flavobacterium kingsejongi OX=1678728 GN=FK004_03965 PE=3 SV=1
MM1 pKa = 7.25 TLANALQLIPSFWDD15 pKa = 3.35 PEE17 pKa = 4.22 EE18 pKa = 4.24 PFPFGKK24 pKa = 9.78 NDD26 pKa = 3.43 HH27 pKa = 6.76 SNHH30 pKa = 5.2 IQRR33 pKa = 11.84 LQEE36 pKa = 3.98 EE37 pKa = 4.75 FKK39 pKa = 11.29 DD40 pKa = 3.98 DD41 pKa = 4.49 LPDD44 pKa = 3.82 SLLEE48 pKa = 4.16 YY49 pKa = 10.42 IRR51 pKa = 11.84 TAVPDD56 pKa = 3.57 EE57 pKa = 5.11 DD58 pKa = 4.57 VDD60 pKa = 4.31 FTNVGSTITLYY71 pKa = 11.24 GIDD74 pKa = 3.73 RR75 pKa = 11.84 LKK77 pKa = 10.93 YY78 pKa = 8.94 LQPGYY83 pKa = 11.08 NYY85 pKa = 10.03 DD86 pKa = 4.1 EE87 pKa = 5.15 KK88 pKa = 10.83 SQSPIEE94 pKa = 4.4 SDD96 pKa = 2.65 WKK98 pKa = 11.03 ASFFVIGDD106 pKa = 3.57 EE107 pKa = 4.84 DD108 pKa = 4.58 GDD110 pKa = 4.68 PILIDD115 pKa = 3.66 LQEE118 pKa = 4.9 PEE120 pKa = 5.04 DD121 pKa = 4.73 GILVLEE127 pKa = 4.86 HH128 pKa = 6.96 GAGSWEE134 pKa = 4.13 YY135 pKa = 11.07 GEE137 pKa = 5.25 SRR139 pKa = 11.84 AATIGQFLLCSAAQHH154 pKa = 5.23 QMLMADD160 pKa = 4.35 EE161 pKa = 5.2 DD162 pKa = 5.14 PIVDD166 pKa = 5.03 DD167 pKa = 4.45 EE168 pKa = 6.44 DD169 pKa = 4.75 GFCLHH174 pKa = 7.21 PEE176 pKa = 3.79 VANWYY181 pKa = 8.3 FPKK184 pKa = 9.48 MKK186 pKa = 9.48 EE187 pKa = 4.22 WAGHH191 pKa = 6.2 YY192 pKa = 9.45 YY193 pKa = 10.01 PEE195 pKa = 4.25 WCSVFDD201 pKa = 3.98 NSS203 pKa = 5.2
Molecular weight: 23.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.778
IPC2_protein 3.961
IPC_protein 3.961
Toseland 3.757
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.846
Rodwell 3.783
Grimsley 3.656
Solomon 3.923
Lehninger 3.884
Nozaki 4.037
DTASelect 4.253
Thurlkill 3.795
EMBOSS 3.859
Sillero 4.075
Patrickios 1.914
IPC_peptide 3.923
IPC2_peptide 4.05
IPC2.peptide.svr19 3.943
Protein with the highest isoelectric point:
>tr|A0A2S1LUD7|A0A2S1LUD7_9FLAO Cyclic nucleotide-binding protein OS=Flavobacterium kingsejongi OX=1678728 GN=FK004_16115 PE=4 SV=1
MM1 pKa = 8.29 DD2 pKa = 3.49 IRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 TGSRR10 pKa = 11.84 TLFLDD15 pKa = 4.25 KK16 pKa = 9.37 MTEE19 pKa = 4.09 KK20 pKa = 10.57 INARR24 pKa = 11.84 MNQRR28 pKa = 11.84 DD29 pKa = 3.54 EE30 pKa = 4.06
Molecular weight: 3.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.31
IPC2_protein 9.414
IPC_protein 10.292
Toseland 10.95
ProMoST 10.906
Dawson 10.965
Bjellqvist 10.73
Wikipedia 11.242
Rodwell 11.023
Grimsley 10.994
Solomon 11.257
Lehninger 11.199
Nozaki 10.921
DTASelect 10.745
Thurlkill 10.921
EMBOSS 11.374
Sillero 10.921
Patrickios 10.906
IPC_peptide 11.257
IPC2_peptide 9.648
IPC2.peptide.svr19 9.015
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3535
0
3535
1176805
30
4271
332.9
37.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.223 ± 0.052
0.767 ± 0.018
5.256 ± 0.035
6.178 ± 0.047
5.019 ± 0.043
6.469 ± 0.046
1.794 ± 0.021
7.748 ± 0.046
7.298 ± 0.076
9.235 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.241 ± 0.026
5.792 ± 0.044
3.642 ± 0.035
3.638 ± 0.025
3.455 ± 0.028
6.23 ± 0.031
6.493 ± 0.099
6.299 ± 0.042
1.007 ± 0.017
4.215 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here