Nocardia panacis
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6591 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3A4KBV5|A0A3A4KBV5_9NOCA Alpha/beta hydrolase OS=Nocardia panacis OX=2340916 GN=D5S18_27705 PE=3 SV=1
MM1 pKa = 7.2 NRR3 pKa = 11.84 IPCYY7 pKa = 10.57 SEE9 pKa = 4.33 FGQVSEE15 pKa = 4.55 IDD17 pKa = 3.47 NLAEE21 pKa = 4.13 RR22 pKa = 11.84 LQGDD26 pKa = 3.65 AYY28 pKa = 11.05 DD29 pKa = 4.04 PVNVPVEE36 pKa = 4.32 CTLTVYY42 pKa = 10.61 VADD45 pKa = 5.07 DD46 pKa = 4.13 EE47 pKa = 5.25 LDD49 pKa = 3.5 EE50 pKa = 4.48 LLPVIMEE57 pKa = 4.74 DD58 pKa = 4.7 LGIDD62 pKa = 3.39 KK63 pKa = 10.64 DD64 pKa = 4.37 GYY66 pKa = 9.66 SAAAGRR72 pKa = 11.84 TVTLGLRR79 pKa = 11.84 DD80 pKa = 3.22 ISSRR84 pKa = 11.84 SEE86 pKa = 3.77 PNGVYY91 pKa = 9.84 QGLIDD96 pKa = 3.69 QYY98 pKa = 10.55 EE99 pKa = 4.44 SQSGADD105 pKa = 3.25
Molecular weight: 11.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.719
IPC_protein 3.681
Toseland 3.478
ProMoST 3.795
Dawson 3.681
Bjellqvist 3.884
Wikipedia 3.617
Rodwell 3.516
Grimsley 3.389
Solomon 3.656
Lehninger 3.617
Nozaki 3.808
DTASelect 4.012
Thurlkill 3.541
EMBOSS 3.63
Sillero 3.808
Patrickios 0.846
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.767
Protein with the highest isoelectric point:
>tr|A0A3A4K6F3|A0A3A4K6F3_9NOCA MerR family transcriptional regulator OS=Nocardia panacis OX=2340916 GN=D5S18_32875 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.76 KK15 pKa = 9.54 KK16 pKa = 9.72 HH17 pKa = 5.63 RR18 pKa = 11.84 KK19 pKa = 7.56 LLRR22 pKa = 11.84 RR23 pKa = 11.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 10.11 LGKK33 pKa = 9.87
Molecular weight: 4.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6591
0
6591
2131699
24
17293
323.4
34.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.671 ± 0.045
0.785 ± 0.011
5.949 ± 0.026
5.49 ± 0.038
2.965 ± 0.017
8.862 ± 0.031
2.24 ± 0.015
4.252 ± 0.022
1.928 ± 0.023
10.285 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.862 ± 0.013
2.028 ± 0.016
5.866 ± 0.028
2.846 ± 0.017
8.086 ± 0.031
5.076 ± 0.019
5.981 ± 0.023
8.264 ± 0.035
1.468 ± 0.015
2.097 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here