Apis mellifera associated microvirus 36
Average proteome isoelectric point is 7.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S8UUC2|A0A3S8UUC2_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 36 OX=2494765 PE=4 SV=1
MM1 pKa = 7.38 NLFNQVQIRR10 pKa = 11.84 RR11 pKa = 11.84 PKK13 pKa = 9.89 RR14 pKa = 11.84 NKK16 pKa = 9.61 FDD18 pKa = 3.91 LSHH21 pKa = 6.29 EE22 pKa = 4.44 KK23 pKa = 10.49 KK24 pKa = 10.77 LSFNMGDD31 pKa = 5.32 LIPIMCQEE39 pKa = 5.36 IIPGDD44 pKa = 3.59 NFRR47 pKa = 11.84 VNTEE51 pKa = 3.59 TFMRR55 pKa = 11.84 LAPMIAPVMHH65 pKa = 6.62 RR66 pKa = 11.84 VNVYY70 pKa = 5.97 THH72 pKa = 6.06 YY73 pKa = 10.7 FFVPTRR79 pKa = 11.84 IIWDD83 pKa = 3.62 EE84 pKa = 3.92 FQDD87 pKa = 4.81 FITGGRR93 pKa = 11.84 EE94 pKa = 4.23 GTSMPISPFFGQSTVEE110 pKa = 3.98 NARR113 pKa = 11.84 GSLLDD118 pKa = 4.0 YY119 pKa = 10.19 MGLPLWPSGSSNDD132 pKa = 3.68 TQISVLPLRR141 pKa = 11.84 AYY143 pKa = 7.91 QTVWDD148 pKa = 4.8 EE149 pKa = 4.1 YY150 pKa = 11.32 FRR152 pKa = 11.84 DD153 pKa = 3.81 QNLTPSLDD161 pKa = 3.13 ISKK164 pKa = 10.86 ASGVMPADD172 pKa = 3.6 AEE174 pKa = 4.3 LFKK177 pKa = 11.18 LLEE180 pKa = 3.95 LRR182 pKa = 11.84 KK183 pKa = 9.69 RR184 pKa = 11.84 AWEE187 pKa = 3.7 KK188 pKa = 11.28 DD189 pKa = 3.61 YY190 pKa = 9.27 FTSALPWSQRR200 pKa = 11.84 GNEE203 pKa = 4.17 ILLPMTPEE211 pKa = 3.55 VNYY214 pKa = 10.57 RR215 pKa = 11.84 PTSDD219 pKa = 3.46 VYY221 pKa = 11.68 YY222 pKa = 10.74 EE223 pKa = 5.75 DD224 pKa = 3.57 GTQANNSSWGIGEE237 pKa = 4.36 GPAGEE242 pKa = 4.74 DD243 pKa = 3.49 GKK245 pKa = 10.79 IVPTPGANILQMRR258 pKa = 11.84 IEE260 pKa = 4.9 NIDD263 pKa = 3.83 SIDD266 pKa = 3.92 NVTTTINDD274 pKa = 2.95 LRR276 pKa = 11.84 RR277 pKa = 11.84 AVRR280 pKa = 11.84 LQEE283 pKa = 3.92 WLEE286 pKa = 3.87 KK287 pKa = 9.97 NARR290 pKa = 11.84 GGARR294 pKa = 11.84 YY295 pKa = 9.32 IEE297 pKa = 4.53 QILHH301 pKa = 6.04 HH302 pKa = 6.67 FGVTSSDD309 pKa = 3.08 ARR311 pKa = 11.84 LQRR314 pKa = 11.84 PEE316 pKa = 3.75 YY317 pKa = 10.49 LGGGKK322 pKa = 9.57 QPVVISEE329 pKa = 4.08 VLQTSEE335 pKa = 4.55 TLSTPQGTMAGHH347 pKa = 6.45 GVSAGTSNGFNRR359 pKa = 11.84 SFEE362 pKa = 3.89 EE363 pKa = 3.49 HH364 pKa = 6.83 GYY366 pKa = 10.35 VIGVMSVLPRR376 pKa = 11.84 TAYY379 pKa = 9.97 QDD381 pKa = 3.59 GVNKK385 pKa = 9.2 MWSRR389 pKa = 11.84 TDD391 pKa = 2.89 KK392 pKa = 10.86 FDD394 pKa = 4.08 YY395 pKa = 10.05 FWPEE399 pKa = 3.44 LANIGEE405 pKa = 4.22 QEE407 pKa = 4.22 VKK409 pKa = 10.43 IKK411 pKa = 10.35 EE412 pKa = 4.33 LYY414 pKa = 10.67 LNPTGAQTNDD424 pKa = 2.76 TFGYY428 pKa = 7.52 QSRR431 pKa = 11.84 YY432 pKa = 10.33 AEE434 pKa = 4.25 YY435 pKa = 10.17 KK436 pKa = 9.33 YY437 pKa = 10.4 MPSTVHH443 pKa = 6.88 GDD445 pKa = 3.03 FRR447 pKa = 11.84 TNLDD451 pKa = 3.39 YY452 pKa = 11.19 WHH454 pKa = 7.1 MGRR457 pKa = 11.84 KK458 pKa = 9.43 FSTEE462 pKa = 3.42 PVLNEE467 pKa = 4.43 DD468 pKa = 5.84 FITADD473 pKa = 3.14 PTHH476 pKa = 7.26 RR477 pKa = 11.84 IFAVTDD483 pKa = 3.8 PDD485 pKa = 3.47 EE486 pKa = 4.78 HH487 pKa = 8.32 KK488 pKa = 10.75 LYY490 pKa = 10.33 CQLYY494 pKa = 9.09 HH495 pKa = 7.31 RR496 pKa = 11.84 IDD498 pKa = 3.64 ALRR501 pKa = 11.84 PMPYY505 pKa = 10.05 FGTPTLL511 pKa = 3.89
Molecular weight: 58.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.362
IPC2_protein 5.359
IPC_protein 5.359
Toseland 5.575
ProMoST 5.601
Dawson 5.474
Bjellqvist 5.55
Wikipedia 5.41
Rodwell 5.423
Grimsley 5.639
Solomon 5.474
Lehninger 5.448
Nozaki 5.664
DTASelect 5.842
Thurlkill 5.677
EMBOSS 5.639
Sillero 5.753
Patrickios 4.05
IPC_peptide 5.486
IPC2_peptide 5.753
IPC2.peptide.svr19 5.729
Protein with the highest isoelectric point:
>tr|A0A3Q8U647|A0A3Q8U647_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 36 OX=2494765 PE=4 SV=1
MM1 pKa = 6.59 YY2 pKa = 10.35 QVGVVLKK9 pKa = 7.92 FAPYY13 pKa = 10.67 SPFNPTSCRR22 pKa = 11.84 PTSQRR27 pKa = 11.84 HH28 pKa = 4.75 AATLIPRR35 pKa = 11.84 GVGATIGRR43 pKa = 11.84 RR44 pKa = 11.84 WAVDD48 pKa = 3.27 RR49 pKa = 11.84 GRR51 pKa = 11.84 KK52 pKa = 8.8 IKK54 pKa = 9.62 PCKK57 pKa = 9.65 GFKK60 pKa = 9.85 IGLKK64 pKa = 9.9 TNTLPLGAPGRR75 pKa = 11.84 TNATCHH81 pKa = 6.68 DD82 pKa = 4.13 GYY84 pKa = 11.5 GRR86 pKa = 11.84 DD87 pKa = 4.25 GLNPWALEE95 pKa = 3.94 APLPLNGII103 pKa = 4.31
Molecular weight: 11.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.78
IPC_protein 10.482
Toseland 10.687
ProMoST 10.35
Dawson 10.789
Bjellqvist 10.496
Wikipedia 10.979
Rodwell 11.096
Grimsley 10.847
Solomon 10.891
Lehninger 10.862
Nozaki 10.701
DTASelect 10.482
Thurlkill 10.687
EMBOSS 11.082
Sillero 10.73
Patrickios 10.862
IPC_peptide 10.891
IPC2_peptide 9.648
IPC2.peptide.svr19 8.443
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1270
103
511
254.0
28.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.402 ± 1.387
0.551 ± 0.261
4.803 ± 0.655
6.929 ± 1.267
3.78 ± 0.708
7.087 ± 0.836
2.835 ± 0.909
5.433 ± 0.534
6.772 ± 2.171
7.638 ± 0.242
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.913 ± 0.424
6.063 ± 1.023
5.591 ± 0.939
4.252 ± 0.714
6.378 ± 0.638
5.118 ± 0.497
6.457 ± 0.502
4.016 ± 0.681
1.732 ± 0.341
4.252 ± 0.388
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here