Leucobacter sp. HDW9C
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3116 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G7XHF1|A0A6G7XHF1_9MICO Metal-sensitive transcriptional regulator OS=Leucobacter sp. HDW9C OX=2714935 GN=G7068_12390 PE=3 SV=1
MM1 pKa = 7.81 AEE3 pKa = 4.02 RR4 pKa = 11.84 MNEE7 pKa = 3.73 GSALFAPDD15 pKa = 4.5 IVSIWIGIFADD26 pKa = 4.58 EE27 pKa = 4.39 SALRR31 pKa = 11.84 AYY33 pKa = 10.56 LEE35 pKa = 4.1 PVYY38 pKa = 10.6 TDD40 pKa = 4.73 DD41 pKa = 4.9 GDD43 pKa = 3.76 TRR45 pKa = 11.84 SDD47 pKa = 3.26 FRR49 pKa = 11.84 ADD51 pKa = 3.42 FDD53 pKa = 4.57 IDD55 pKa = 3.97 YY56 pKa = 11.03 DD57 pKa = 4.56 DD58 pKa = 5.85 DD59 pKa = 3.83 FAEE62 pKa = 5.39 ADD64 pKa = 3.43 LSTNLVEE71 pKa = 5.17 QLPQHH76 pKa = 6.68 SYY78 pKa = 11.36 GSTIDD83 pKa = 3.41 AAALADD89 pKa = 3.28 IRR91 pKa = 11.84 RR92 pKa = 11.84 YY93 pKa = 9.74 PSANALLLLYY103 pKa = 10.64 NIDD106 pKa = 3.85 ASAFQNHH113 pKa = 7.38 PGRR116 pKa = 11.84 MTLLGTYY123 pKa = 9.18 AYY125 pKa = 10.91 SPTTPDD131 pKa = 3.02 LSRR134 pKa = 11.84 YY135 pKa = 7.72 YY136 pKa = 11.15
Molecular weight: 15.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.723
IPC2_protein 3.897
IPC_protein 3.884
Toseland 3.668
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.859
Rodwell 3.719
Grimsley 3.567
Solomon 3.884
Lehninger 3.846
Nozaki 4.012
DTASelect 4.291
Thurlkill 3.732
EMBOSS 3.859
Sillero 4.012
Patrickios 1.036
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.895
Protein with the highest isoelectric point:
>tr|A0A6G7XG20|A0A6G7XG20_9MICO SulP family inorganic anion transporter OS=Leucobacter sp. HDW9C OX=2714935 GN=G7068_10390 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.5 VHH17 pKa = 5.46 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AIVAARR35 pKa = 11.84 RR36 pKa = 11.84 GKK38 pKa = 10.45 GRR40 pKa = 11.84 SKK42 pKa = 10.13 LTAA45 pKa = 4.04
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3116
0
3116
1090697
18
5174
350.0
37.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.331 ± 0.056
0.571 ± 0.011
5.668 ± 0.039
6.027 ± 0.054
3.265 ± 0.031
8.796 ± 0.055
1.783 ± 0.023
4.779 ± 0.036
2.902 ± 0.041
9.747 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.876 ± 0.021
2.725 ± 0.035
5.201 ± 0.033
3.187 ± 0.025
6.219 ± 0.066
6.445 ± 0.041
6.547 ± 0.08
8.476 ± 0.056
1.4 ± 0.02
2.054 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here