Leucobacter sp. HDW9C

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leucobacter

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3116 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A6G7XHF1|A0A6G7XHF1_9MICO Metal-sensitive transcriptional regulator OS=Leucobacter sp. HDW9C OX=2714935 GN=G7068_12390 PE=3 SV=1
MM1 pKa = 7.81AEE3 pKa = 4.02RR4 pKa = 11.84MNEE7 pKa = 3.73GSALFAPDD15 pKa = 4.5IVSIWIGIFADD26 pKa = 4.58EE27 pKa = 4.39SALRR31 pKa = 11.84AYY33 pKa = 10.56LEE35 pKa = 4.1PVYY38 pKa = 10.6TDD40 pKa = 4.73DD41 pKa = 4.9GDD43 pKa = 3.76TRR45 pKa = 11.84SDD47 pKa = 3.26FRR49 pKa = 11.84ADD51 pKa = 3.42FDD53 pKa = 4.57IDD55 pKa = 3.97YY56 pKa = 11.03DD57 pKa = 4.56DD58 pKa = 5.85DD59 pKa = 3.83FAEE62 pKa = 5.39ADD64 pKa = 3.43LSTNLVEE71 pKa = 5.17QLPQHH76 pKa = 6.68SYY78 pKa = 11.36GSTIDD83 pKa = 3.41AAALADD89 pKa = 3.28IRR91 pKa = 11.84RR92 pKa = 11.84YY93 pKa = 9.74PSANALLLLYY103 pKa = 10.64NIDD106 pKa = 3.85ASAFQNHH113 pKa = 7.38PGRR116 pKa = 11.84MTLLGTYY123 pKa = 9.18AYY125 pKa = 10.91SPTTPDD131 pKa = 3.02LSRR134 pKa = 11.84YY135 pKa = 7.72YY136 pKa = 11.15

Molecular weight:
15.18 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A6G7XG20|A0A6G7XG20_9MICO SulP family inorganic anion transporter OS=Leucobacter sp. HDW9C OX=2714935 GN=G7068_10390 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.5VHH17 pKa = 5.46GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.45GRR40 pKa = 11.84SKK42 pKa = 10.13LTAA45 pKa = 4.04

Molecular weight:
5.18 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3116

0

3116

1090697

18

5174

350.0

37.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.331 ± 0.056

0.571 ± 0.011

5.668 ± 0.039

6.027 ± 0.054

3.265 ± 0.031

8.796 ± 0.055

1.783 ± 0.023

4.779 ± 0.036

2.902 ± 0.041

9.747 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.876 ± 0.021

2.725 ± 0.035

5.201 ± 0.033

3.187 ± 0.025

6.219 ± 0.066

6.445 ± 0.041

6.547 ± 0.08

8.476 ± 0.056

1.4 ± 0.02

2.054 ± 0.017

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski