Amino acid dipepetide frequency for Leucobacter sp. HDW9C

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.537AlaAla: 17.537 ± 0.203	0.676AlaCys: 0.676 ± 0.027	6.972AlaAsp: 6.972 ± 0.099	8.184AlaGlu: 8.184 ± 0.128	3.693AlaPhe: 3.693 ± 0.054	10.875AlaGly: 10.875 ± 0.142	2.054AlaHis: 2.054 ± 0.053	5.946AlaIle: 5.946 ± 0.091	3.794AlaLys: 3.794 ± 0.08	12.168AlaLeu: 12.168 ± 0.153	2.552AlaMet: 2.552 ± 0.051	3.115AlaAsn: 3.115 ± 0.07	5.831AlaPro: 5.831 ± 0.104	4.124AlaGln: 4.124 ± 0.08	7.012AlaArg: 7.012 ± 0.107	7.493AlaSer: 7.493 ± 0.127	7.657AlaThr: 7.657 ± 0.131	9.922AlaVal: 9.922 ± 0.101	1.494AlaTrp: 1.494 ± 0.041	2.206AlaTyr: 2.206 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	0.682CysAla: 0.682 ± 0.026	0.057CysCys: 0.057 ± 0.008	0.395CysAsp: 0.395 ± 0.021	0.358CysGlu: 0.358 ± 0.022	0.181CysPhe: 0.181 ± 0.013	0.581CysGly: 0.581 ± 0.021	0.119CysHis: 0.119 ± 0.012	0.211CysIle: 0.211 ± 0.014	0.114CysLys: 0.114 ± 0.011	0.464CysLeu: 0.464 ± 0.023	0.109CysMet: 0.109 ± 0.009	0.134CysAsn: 0.134 ± 0.011	0.303CysPro: 0.303 ± 0.021	0.16CysGln: 0.16 ± 0.014	0.309CysArg: 0.309 ± 0.017	0.413CysSer: 0.413 ± 0.021	0.432CysThr: 0.432 ± 0.024	0.503CysVal: 0.503 ± 0.027	0.069CysTrp: 0.069 ± 0.008	0.12CysTyr: 0.12 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	6.967AspAla: 6.967 ± 0.099	0.267AspCys: 0.267 ± 0.018	3.06AspAsp: 3.06 ± 0.069	3.688AspGlu: 3.688 ± 0.077	1.808AspPhe: 1.808 ± 0.042	5.36AspGly: 5.36 ± 0.09	1.082AspHis: 1.082 ± 0.031	2.22AspIle: 2.22 ± 0.052	1.362AspLys: 1.362 ± 0.038	5.563AspLeu: 5.563 ± 0.075	0.852AspMet: 0.852 ± 0.03	1.318AspAsn: 1.318 ± 0.04	4.408AspPro: 4.408 ± 0.07	1.686AspGln: 1.686 ± 0.047	3.821AspArg: 3.821 ± 0.065	3.236AspSer: 3.236 ± 0.063	3.272AspThr: 3.272 ± 0.068	4.515AspVal: 4.515 ± 0.081	0.853AspTrp: 0.853 ± 0.024	1.343AspTyr: 1.343 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	7.46GluAla: 7.46 ± 0.119	0.354GluCys: 0.354 ± 0.024	2.851GluAsp: 2.851 ± 0.056	3.308GluGlu: 3.308 ± 0.089	1.993GluPhe: 1.993 ± 0.044	4.328GluGly: 4.328 ± 0.081	1.385GluHis: 1.385 ± 0.039	3.11GluIle: 3.11 ± 0.064	1.836GluLys: 1.836 ± 0.054	6.814GluLeu: 6.814 ± 0.114	1.205GluMet: 1.205 ± 0.031	1.55GluAsn: 1.55 ± 0.041	2.844GluPro: 2.844 ± 0.071	2.656GluGln: 2.656 ± 0.06	4.692GluArg: 4.692 ± 0.096	3.439GluSer: 3.439 ± 0.055	3.459GluThr: 3.459 ± 0.058	4.844GluVal: 4.844 ± 0.078	0.892GluTrp: 0.892 ± 0.034	1.251GluTyr: 1.251 ± 0.032	0.0GluXaa: 0.0 ± 0.0
Phe	4.256PheAla: 4.256 ± 0.071	0.19PheCys: 0.19 ± 0.014	2.281PheAsp: 2.281 ± 0.044	2.084PheGlu: 2.084 ± 0.048	1.105PhePhe: 1.105 ± 0.036	3.452PheGly: 3.452 ± 0.061	0.511PheHis: 0.511 ± 0.024	1.396PheIle: 1.396 ± 0.039	0.738PheLys: 0.738 ± 0.028	2.652PheLeu: 2.652 ± 0.064	0.573PheMet: 0.573 ± 0.022	0.819PheAsn: 0.819 ± 0.028	1.403PhePro: 1.403 ± 0.041	0.851PheGln: 0.851 ± 0.027	1.684PheArg: 1.684 ± 0.04	2.175PheSer: 2.175 ± 0.041	2.442PheThr: 2.442 ± 0.055	2.84PheVal: 2.84 ± 0.058	0.464PheTrp: 0.464 ± 0.022	0.73PheTyr: 0.73 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	9.872GlyAla: 9.872 ± 0.116	0.58GlyCys: 0.58 ± 0.029	4.871GlyAsp: 4.871 ± 0.076	5.208GlyGlu: 5.208 ± 0.074	3.218GlyPhe: 3.218 ± 0.053	7.473GlyGly: 7.473 ± 0.112	1.464GlyHis: 1.464 ± 0.04	4.849GlyIle: 4.849 ± 0.074	2.98GlyLys: 2.98 ± 0.068	8.283GlyLeu: 8.283 ± 0.108	1.871GlyMet: 1.871 ± 0.05	2.751GlyAsn: 2.751 ± 0.099	3.321GlyPro: 3.321 ± 0.054	2.716GlyGln: 2.716 ± 0.063	4.785GlyArg: 4.785 ± 0.089	6.43GlySer: 6.43 ± 0.109	5.971GlyThr: 5.971 ± 0.133	7.681GlyVal: 7.681 ± 0.09	1.489GlyTrp: 1.489 ± 0.044	2.145GlyTyr: 2.145 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	1.94HisAla: 1.94 ± 0.046	0.12HisCys: 0.12 ± 0.01	1.012HisAsp: 1.012 ± 0.027	1.127HisGlu: 1.127 ± 0.034	0.592HisPhe: 0.592 ± 0.024	1.606HisGly: 1.606 ± 0.045	0.436HisHis: 0.436 ± 0.023	0.685HisIle: 0.685 ± 0.027	0.41HisLys: 0.41 ± 0.02	1.869HisLeu: 1.869 ± 0.05	0.309HisMet: 0.309 ± 0.017	0.46HisAsn: 0.46 ± 0.021	1.229HisPro: 1.229 ± 0.032	0.508HisGln: 0.508 ± 0.022	1.281HisArg: 1.281 ± 0.04	1.108HisSer: 1.108 ± 0.032	1.108HisThr: 1.108 ± 0.042	1.322HisVal: 1.322 ± 0.041	0.271HisTrp: 0.271 ± 0.015	0.436HisTyr: 0.436 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.855IleAla: 6.855 ± 0.098	0.277IleCys: 0.277 ± 0.016	3.361IleAsp: 3.361 ± 0.057	3.213IleGlu: 3.213 ± 0.065	1.257IlePhe: 1.257 ± 0.036	4.498IleGly: 4.498 ± 0.074	0.667IleHis: 0.667 ± 0.03	1.964IleIle: 1.964 ± 0.053	1.092IleLys: 1.092 ± 0.035	4.094IleLeu: 4.094 ± 0.074	0.731IleMet: 0.731 ± 0.024	1.185IleAsn: 1.185 ± 0.036	2.634IlePro: 2.634 ± 0.055	1.143IleGln: 1.143 ± 0.032	2.592IleArg: 2.592 ± 0.047	2.975IleSer: 2.975 ± 0.048	3.495IleThr: 3.495 ± 0.063	4.347IleVal: 4.347 ± 0.074	0.536IleTrp: 0.536 ± 0.021	0.875IleTyr: 0.875 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	3.01LysAla: 3.01 ± 0.059	0.127LysCys: 0.127 ± 0.011	1.597LysAsp: 1.597 ± 0.047	1.389LysGlu: 1.389 ± 0.045	0.91LysPhe: 0.91 ± 0.035	2.043LysGly: 2.043 ± 0.053	0.6LysHis: 0.6 ± 0.026	1.398LysIle: 1.398 ± 0.037	1.245LysLys: 1.245 ± 0.049	2.981LysLeu: 2.981 ± 0.06	0.615LysMet: 0.615 ± 0.027	1.009LysAsn: 1.009 ± 0.043	1.939LysPro: 1.939 ± 0.049	1.114LysGln: 1.114 ± 0.033	2.022LysArg: 2.022 ± 0.045	1.754LysSer: 1.754 ± 0.041	1.917LysThr: 1.917 ± 0.054	2.329LysVal: 2.329 ± 0.06	0.381LysTrp: 0.381 ± 0.02	0.643LysTyr: 0.643 ± 0.028	0.0LysXaa: 0.0 ± 0.0
Leu	12.913LeuAla: 12.913 ± 0.133	0.533LeuCys: 0.533 ± 0.025	5.702LeuAsp: 5.702 ± 0.09	5.469LeuGlu: 5.469 ± 0.091	2.85LeuPhe: 2.85 ± 0.068	8.992LeuGly: 8.992 ± 0.126	1.63LeuHis: 1.63 ± 0.039	4.728LeuIle: 4.728 ± 0.089	2.493LeuLys: 2.493 ± 0.057	9.404LeuLeu: 9.404 ± 0.152	1.643LeuMet: 1.643 ± 0.045	2.522LeuAsn: 2.522 ± 0.052	5.209LeuPro: 5.209 ± 0.079	2.73LeuGln: 2.73 ± 0.058	6.642LeuArg: 6.642 ± 0.103	6.691LeuSer: 6.691 ± 0.092	6.522LeuThr: 6.522 ± 0.096	8.038LeuVal: 8.038 ± 0.116	1.176LeuTrp: 1.176 ± 0.041	1.585LeuTyr: 1.585 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	1.973MetAla: 1.973 ± 0.048	0.119MetCys: 0.119 ± 0.01	0.757MetAsp: 0.757 ± 0.026	0.793MetGlu: 0.793 ± 0.028	0.569MetPhe: 0.569 ± 0.023	1.366MetGly: 1.366 ± 0.042	0.364MetHis: 0.364 ± 0.019	0.947MetIle: 0.947 ± 0.03	0.626MetLys: 0.626 ± 0.027	2.103MetLeu: 2.103 ± 0.054	0.333MetMet: 0.333 ± 0.017	0.663MetAsn: 0.663 ± 0.022	1.096MetPro: 1.096 ± 0.033	0.661MetGln: 0.661 ± 0.026	1.275MetArg: 1.275 ± 0.042	1.616MetSer: 1.616 ± 0.035	1.553MetThr: 1.553 ± 0.04	1.353MetVal: 1.353 ± 0.041	0.213MetTrp: 0.213 ± 0.014	0.38MetTyr: 0.38 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	3.111AsnAla: 3.111 ± 0.058	0.167AsnCys: 0.167 ± 0.015	1.541AsnAsp: 1.541 ± 0.042	1.441AsnGlu: 1.441 ± 0.043	0.837AsnPhe: 0.837 ± 0.03	2.739AsnGly: 2.739 ± 0.079	0.442AsnHis: 0.442 ± 0.02	1.145AsnIle: 1.145 ± 0.035	0.763AsnLys: 0.763 ± 0.029	2.391AsnLeu: 2.391 ± 0.048	0.448AsnMet: 0.448 ± 0.021	0.875AsnAsn: 0.875 ± 0.04	1.988AsnPro: 1.988 ± 0.054	0.798AsnGln: 0.798 ± 0.028	1.538AsnArg: 1.538 ± 0.041	1.67AsnSer: 1.67 ± 0.045	2.014AsnThr: 2.014 ± 0.061	2.164AsnVal: 2.164 ± 0.062	0.435AsnTrp: 0.435 ± 0.02	0.747AsnTyr: 0.747 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	6.301ProAla: 6.301 ± 0.11	0.206ProCys: 0.206 ± 0.013	3.122ProAsp: 3.122 ± 0.058	4.265ProGlu: 4.265 ± 0.07	1.694ProPhe: 1.694 ± 0.045	5.074ProGly: 5.074 ± 0.099	0.927ProHis: 0.927 ± 0.033	2.222ProIle: 2.222 ± 0.048	1.778ProLys: 1.778 ± 0.048	4.665ProLeu: 4.665 ± 0.08	0.877ProMet: 0.877 ± 0.032	1.486ProAsn: 1.486 ± 0.037	1.955ProPro: 1.955 ± 0.054	1.68ProGln: 1.68 ± 0.05	2.661ProArg: 2.661 ± 0.047	3.334ProSer: 3.334 ± 0.063	3.354ProThr: 3.354 ± 0.08	4.753ProVal: 4.753 ± 0.074	0.703ProTrp: 0.703 ± 0.027	0.956ProTyr: 0.956 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	3.826GlnAla: 3.826 ± 0.064	0.147GlnCys: 0.147 ± 0.012	1.496GlnAsp: 1.496 ± 0.044	1.557GlnGlu: 1.557 ± 0.043	1.018GlnPhe: 1.018 ± 0.026	2.259GlnGly: 2.259 ± 0.053	0.736GlnHis: 0.736 ± 0.031	1.676GlnIle: 1.676 ± 0.039	0.915GlnLys: 0.915 ± 0.027	3.689GlnLeu: 3.689 ± 0.061	0.647GlnMet: 0.647 ± 0.026	0.954GlnAsn: 0.954 ± 0.031	1.691GlnPro: 1.691 ± 0.053	1.549GlnGln: 1.549 ± 0.046	2.376GlnArg: 2.376 ± 0.059	1.837GlnSer: 1.837 ± 0.043	1.806GlnThr: 1.806 ± 0.034	2.528GlnVal: 2.528 ± 0.054	0.441GlnTrp: 0.441 ± 0.022	0.722GlnTyr: 0.722 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	7.166ArgAla: 7.166 ± 0.107	0.321ArgCys: 0.321 ± 0.016	3.627ArgAsp: 3.627 ± 0.063	4.362ArgGlu: 4.362 ± 0.092	2.331ArgPhe: 2.331 ± 0.052	4.774ArgGly: 4.774 ± 0.082	1.162ArgHis: 1.162 ± 0.039	3.345ArgIle: 3.345 ± 0.062	1.803ArgLys: 1.803 ± 0.045	6.078ArgLeu: 6.078 ± 0.104	1.394ArgMet: 1.394 ± 0.038	1.522ArgAsn: 1.522 ± 0.04	2.74ArgPro: 2.74 ± 0.056	1.881ArgGln: 1.881 ± 0.043	4.572ArgArg: 4.572 ± 0.077	3.794ArgSer: 3.794 ± 0.069	3.473ArgThr: 3.473 ± 0.054	5.459ArgVal: 5.459 ± 0.098	0.99ArgTrp: 0.99 ± 0.034	1.395ArgTyr: 1.395 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	7.627SerAla: 7.627 ± 0.109	0.42SerCys: 0.42 ± 0.023	3.553SerAsp: 3.553 ± 0.071	3.772SerGlu: 3.772 ± 0.061	2.307SerPhe: 2.307 ± 0.042	6.966SerGly: 6.966 ± 0.12	1.113SerHis: 1.113 ± 0.038	2.927SerIle: 2.927 ± 0.061	1.859SerLys: 1.859 ± 0.05	5.879SerLeu: 5.879 ± 0.083	1.281SerMet: 1.281 ± 0.039	1.814SerAsn: 1.814 ± 0.049	3.225SerPro: 3.225 ± 0.059	1.936SerGln: 1.936 ± 0.042	3.751SerArg: 3.751 ± 0.064	4.198SerSer: 4.198 ± 0.076	4.107SerThr: 4.107 ± 0.094	5.263SerVal: 5.263 ± 0.092	1.015SerTrp: 1.015 ± 0.034	1.439SerTyr: 1.439 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	7.719ThrAla: 7.719 ± 0.146	0.379ThrCys: 0.379 ± 0.022	3.523ThrAsp: 3.523 ± 0.099	3.564ThrGlu: 3.564 ± 0.062	2.1ThrPhe: 2.1 ± 0.05	6.225ThrGly: 6.225 ± 0.12	1.108ThrHis: 1.108 ± 0.032	3.256ThrIle: 3.256 ± 0.068	1.831ThrLys: 1.831 ± 0.042	6.356ThrLeu: 6.356 ± 0.102	1.046ThrMet: 1.046 ± 0.032	1.857ThrAsn: 1.857 ± 0.062	4.287ThrPro: 4.287 ± 0.095	1.908ThrGln: 1.908 ± 0.041	3.465ThrArg: 3.465 ± 0.059	4.013ThrSer: 4.013 ± 0.082	4.131ThrThr: 4.131 ± 0.099	6.48ThrVal: 6.48 ± 0.2	0.855ThrTrp: 0.855 ± 0.031	1.368ThrTyr: 1.368 ± 0.059	0.0ThrXaa: 0.0 ± 0.0
Val	10.423ValAla: 10.423 ± 0.124	0.531ValCys: 0.531 ± 0.023	4.924ValAsp: 4.924 ± 0.083	4.724ValGlu: 4.724 ± 0.072	2.886ValPhe: 2.886 ± 0.068	6.474ValGly: 6.474 ± 0.086	1.434ValHis: 1.434 ± 0.04	4.275ValIle: 4.275 ± 0.065	2.382ValLys: 2.382 ± 0.076	8.488ValLeu: 8.488 ± 0.116	1.499ValMet: 1.499 ± 0.04	2.191ValAsn: 2.191 ± 0.052	4.261ValPro: 4.261 ± 0.07	2.521ValGln: 2.521 ± 0.05	5.04ValArg: 5.04 ± 0.076	5.929ValSer: 5.929 ± 0.12	6.507ValThr: 6.507 ± 0.187	7.64ValVal: 7.64 ± 0.114	1.109ValTrp: 1.109 ± 0.036	1.517ValTyr: 1.517 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	1.43TrpAla: 1.43 ± 0.04	0.097TrpCys: 0.097 ± 0.009	0.741TrpAsp: 0.741 ± 0.028	0.61TrpGlu: 0.61 ± 0.028	0.555TrpPhe: 0.555 ± 0.026	1.029TrpGly: 1.029 ± 0.038	0.291TrpHis: 0.291 ± 0.016	0.768TrpIle: 0.768 ± 0.031	0.403TrpLys: 0.403 ± 0.02	1.448TrpLeu: 1.448 ± 0.044	0.44TrpMet: 0.44 ± 0.026	0.477TrpAsn: 0.477 ± 0.021	0.646TrpPro: 0.646 ± 0.027	0.602TrpGln: 0.602 ± 0.026	1.009TrpArg: 1.009 ± 0.032	0.967TrpSer: 0.967 ± 0.032	0.762TrpThr: 0.762 ± 0.025	1.167TrpVal: 1.167 ± 0.04	0.302TrpTrp: 0.302 ± 0.018	0.259TrpTyr: 0.259 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.238TyrAla: 2.238 ± 0.046	0.147TyrCys: 0.147 ± 0.011	1.294TyrAsp: 1.294 ± 0.05	1.158TyrGlu: 1.158 ± 0.03	0.744TyrPhe: 0.744 ± 0.03	1.845TyrGly: 1.845 ± 0.041	0.303TyrHis: 0.303 ± 0.018	0.722TyrIle: 0.722 ± 0.027	0.6TyrLys: 0.6 ± 0.028	2.083TyrLeu: 2.083 ± 0.049	0.335TyrMet: 0.335 ± 0.018	0.551TyrAsn: 0.551 ± 0.023	1.004TyrPro: 1.004 ± 0.034	0.645TyrGln: 0.645 ± 0.025	1.659TyrArg: 1.659 ± 0.05	1.38TyrSer: 1.38 ± 0.039	1.486TyrThr: 1.486 ± 0.063	1.608TyrVal: 1.608 ± 0.04	0.314TyrTrp: 0.314 ± 0.016	0.422TyrTyr: 0.422 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3116 proteins (1090697 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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