Rhodococcus phage E3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 220 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M9MUF0|M9MUF0_9CAUD Uncharacterized protein OS=Rhodococcus phage E3 OX=1007869 GN=gp80 PE=4 SV=1
MM1 pKa = 7.86TDD3 pKa = 3.05THH5 pKa = 6.61LHH7 pKa = 5.36TVTITGTEE15 pKa = 4.13DD16 pKa = 3.07SPKK19 pKa = 10.13IEE21 pKa = 4.47FVCHH25 pKa = 6.34GDD27 pKa = 3.55RR28 pKa = 11.84DD29 pKa = 4.49SDD31 pKa = 3.8CHH33 pKa = 7.12SYY35 pKa = 10.64PDD37 pKa = 4.19CRR39 pKa = 11.84CEE41 pKa = 3.49RR42 pKa = 11.84WDD44 pKa = 4.48RR45 pKa = 11.84DD46 pKa = 3.66DD47 pKa = 4.94HH48 pKa = 5.6EE49 pKa = 5.44HH50 pKa = 6.93PFVPHH55 pKa = 6.1DD56 pKa = 3.95QCWMQAWFDD65 pKa = 3.9EE66 pKa = 4.82GATDD70 pKa = 5.31PMAEE74 pKa = 4.48TLDD77 pKa = 3.68EE78 pKa = 4.66CPDD81 pKa = 3.78LKK83 pKa = 11.1VGMSGPIEE91 pKa = 4.29SVFLFDD97 pKa = 4.04YY98 pKa = 10.95IEE100 pKa = 3.91WEE102 pKa = 4.23FAA104 pKa = 3.48

Molecular weight: 12.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M9MUI0|M9MUI0_9CAUD Uncharacterized protein OS=Rhodococcus phage E3 OX=1007869 GN=gp147 PE=4 SV=1
MM1 pKa = 7.95PDD3 pKa = 2.79ITTEE7 pKa = 3.95QPQRR11 pKa = 11.84SCSRR15 pKa = 11.84TALPAPIVDD24 pKa = 6.04LISQGIPLKK33 pKa = 10.77EE34 pKa = 3.9FDD36 pKa = 3.21RR37 pKa = 11.84SGRR40 pKa = 11.84VAPVLIWCAVRR51 pKa = 11.84RR52 pKa = 11.84TAASAQANGWTRR64 pKa = 11.84MQWFNHH70 pKa = 4.94VAADD74 pKa = 3.82TNILFWQLKK83 pKa = 7.76VGNPRR88 pKa = 11.84RR89 pKa = 11.84PKK91 pKa = 9.69PRR93 pKa = 11.84EE94 pKa = 3.65LKK96 pKa = 10.18KK97 pKa = 10.87VYY99 pKa = 10.19RR100 pKa = 11.84DD101 pKa = 3.75LDD103 pKa = 3.99KK104 pKa = 11.39AWDD107 pKa = 3.36ASVRR111 pKa = 11.84YY112 pKa = 9.03RR113 pKa = 11.84ASEE116 pKa = 3.99EE117 pKa = 3.64QAAPATAAEE126 pKa = 4.29VSRR129 pKa = 11.84RR130 pKa = 11.84SNAVTRR136 pKa = 11.84LVQRR140 pKa = 11.84HH141 pKa = 5.78ADD143 pKa = 3.74RR144 pKa = 11.84MQLSYY149 pKa = 10.31TEE151 pKa = 4.13RR152 pKa = 11.84QVLAFAAAEE161 pKa = 3.97TRR163 pKa = 11.84RR164 pKa = 11.84YY165 pKa = 9.63GWLNVTLPARR175 pKa = 11.84RR176 pKa = 11.84VAQAIGRR183 pKa = 11.84SAMAAHH189 pKa = 6.68KK190 pKa = 10.06VLRR193 pKa = 11.84RR194 pKa = 11.84LAEE197 pKa = 3.77QGLLVQVARR206 pKa = 11.84GRR208 pKa = 11.84AGTTVNGGSGSAAIYY223 pKa = 8.16QIPVDD228 pKa = 3.1IATFTRR234 pKa = 11.84EE235 pKa = 4.01DD236 pKa = 3.79GAEE239 pKa = 4.03QPHH242 pKa = 6.44SGPVGSLHH250 pKa = 6.55ARR252 pKa = 11.84IGTGHH257 pKa = 7.25DD258 pKa = 3.44PVAAAAGDD266 pKa = 3.81AQVGRR271 pKa = 11.84RR272 pKa = 11.84AGADD276 pKa = 3.33VPGDD280 pKa = 3.4ARR282 pKa = 11.84DD283 pKa = 3.78PGGHH287 pKa = 5.85GG288 pKa = 3.29

Molecular weight: 31.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

220

0

220

43866

40

1161

199.4

21.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.231 ± 0.218

0.889 ± 0.08

6.579 ± 0.158

6.513 ± 0.225

2.749 ± 0.104

8.015 ± 0.228

2.259 ± 0.111

4.514 ± 0.13

3.707 ± 0.116

7.812 ± 0.175

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.485 ± 0.111

3.089 ± 0.142

5.394 ± 0.17

4.076 ± 0.181

6.875 ± 0.214

6.255 ± 0.165

6.299 ± 0.23

7.427 ± 0.197

1.701 ± 0.069

3.13 ± 0.121
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski